SRR1527227_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527227_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1744277
Sequences flagged as poor quality	0
Sequence length	52
%GC	60

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	10047	0.57599796362619	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	9629	0.5520338799399407	No Hit
GTCGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	8179	0.46890488150677906	No Hit
GTCGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5664	0.32471906698305375	No Hit
GTCGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5549	0.31812607745214777	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5385	0.3087239010776385	No Hit
GTCGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	5049	0.2894609055786438	No Hit
GTCGGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	4735	0.2714591776420832	No Hit
GTCGGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	4029	0.2309839549566955	No Hit
GTCGGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3867	0.22169643926968022	No Hit
GTCGGTGGGTCATTCAGATTGCCATCATCTCTATCTTCAAGTCCTATCCAAC	3694	0.21177828980144783	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	3613	0.20713453195794015	No Hit
GTCGGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	3611	0.20701987127044616	No Hit
GTCGGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2983	0.17101641539732507	No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2940	0.16855121061620373	No Hit
GTCGGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2938	0.1684365499287097	No Hit
GTCGGTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	2904	0.16648731824131144	No Hit
GTCGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2902	0.16637265755381744	No Hit
GTCGGTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	2604	0.14928821511720902	No Hit
GTCGGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	2572	0.14745364411730474	No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2442	0.14000069943019372	No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2401	0.13765015533656638	No Hit
GTCGGTGGGCTGTAGGTGTCTGTGTGGGTGTGGGTGTGCTATAGGTATCTGT	2398	0.13747816430532536	No Hit
GTCGGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	2368	0.1357582539929151	No Hit
GTCGGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2301	0.13191712096186559	No Hit
GTCGGTGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	2201	0.1261840865871648	No Hit
GTCGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	2180	0.12498014936847761	No Hit
GTCGGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	2008	0.11511933024399221	No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1999	0.11460335715026913	No Hit
GTCGGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1894	0.10858367105683328	No Hit
GTCGGTGGGGTTCCCCTGTTTTGAGTATTTCTGTCCCTGGAATAACCTTTTA	1805	0.10348127046334957	No Hit
GTCGGTGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA	1751	0.10038543190101112	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAA	40	5.6134013E-9	46.000004	15
GATAACG	20	6.3123566E-4	46.000004	19
TAGCGTA	20	6.3123566E-4	46.000004	24
CGTTATG	40	5.6134013E-9	46.000004	45
ATCCGGT	40	5.6134013E-9	46.000004	16
CGGATTA	20	6.3123566E-4	46.000004	17
CGTACTA	20	6.3123566E-4	46.000004	33
TTACGGG	20	6.3123566E-4	46.000004	14
ATAACGA	20	6.3123566E-4	46.000004	20
ATACGTC	20	6.3123566E-4	46.000004	22
AGCTATC	20	6.3123566E-4	46.000004	16
ATTGCGA	20	6.3123566E-4	46.000004	10
TATACCG	20	6.3123566E-4	46.000004	13
CCGTTTA	20	6.3123566E-4	46.000004	32
TACGTCT	20	6.3123566E-4	46.000004	23
TACCGTT	20	6.3123566E-4	46.000004	15
TACCGAC	40	5.6134013E-9	46.000004	29
CGGAATA	45	3.110472E-10	46.0	32
AACGTAA	25	3.417889E-5	46.0	19
ATAGGGT	25	3.417889E-5	46.0	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content