FastQCFastQC Report
Fri 17 Jun 2016
SRR1527226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691744
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG98270.580879849433484No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC91600.5414530803714982No Hit
GTCGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG81390.4811011595134962No Hit
GTCGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT58940.348397866343844No Hit
GTCGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC56030.331196682240339No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT52740.31174929540166835No Hit
GTCGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC47520.2808935630922882No Hit
GTCGGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG45650.26983988121134167No Hit
GTCGGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG35460.20960618154992716No Hit
GTCGGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA35300.20866041197722587No Hit
GTCGGTGGGTCATTCAGATTGCCATCATCTCTATCTTCAAGTCCTATCCAAC35270.20848308018234438No Hit
GTCGGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT34780.2055866608659466No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC34540.20416800650689465No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC28920.17094785026576126No Hit
GTCGGTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC27500.16255414530803716No Hit
GTCGGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG27300.16137193334216052No Hit
GTCGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG26690.15776618684623678No Hit
GTCGGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC25820.15262356479467343No Hit
GTCGGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT25210.1490178182987497No Hit
GTCGGTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC24850.14688983676017175No Hit
GTCGGTGGGCTGTAGGTGTCTGTGTGGGTGTGGGTGTGCTATAGGTATCTGT24480.1447027446233No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA24120.14257476308472203No Hit
GTCGGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT23350.138023247016097No Hit
GTCGGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC23170.13695925624680805No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23140.13678192445192652No Hit
GTCGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA21780.12874288308396542No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT21150.12501891539145402No Hit
GTCGGTGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC19990.11816208598936954No Hit
GTCGGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA19030.1124874685531617No Hit
GTCGGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA17870.10563063915107723No Hit
GTCGGTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG17290.10220222445003499No Hit
GTCGGTGGGGTTCCCCTGTTTTGAGTATTTCTGTCCCTGGAATAACCTTTTA17180.10155200786880285No Hit
GTCGGTGGGATCTTACAGTTACCTCATTTTTCCCATGTATGTATTTGAGAAA17010.10054712769780771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC551.8189894E-1246.00000440
ACGACAC351.0199619E-746.00000438
GATAACG253.4178447E-546.019
TTTACGG253.4178447E-546.013
CAACGAA301.8619285E-646.020
ACGCATC206.3123036E-446.040
CGTGCAA301.8619285E-646.040
TACGAAT206.3123036E-446.023
CGTACTC253.4178447E-546.021
CGTAAGA206.3123036E-446.017
CGTAACC206.3123036E-446.027
ATAACGT206.3123036E-446.015
ATAACGC405.6134013E-946.039
ATAACGA253.4178447E-546.020
CGCACTA301.8619285E-646.033
ACGTAAT301.8619285E-646.020
TATACGT206.3123036E-446.034
TCGAAAC206.3123036E-446.030
ATCGATT206.3123036E-446.012
GTACGAC253.4178447E-546.010
Produced by FastQC (version 0.11.5)