##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527226_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1691744
Sequences flagged as poor quality	0
Sequence length	52
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64228216562317	31.0	31.0	33.0	31.0	33.0
2	31.54123023341593	31.0	31.0	33.0	30.0	34.0
3	32.4491926674485	33.0	31.0	34.0	31.0	34.0
4	36.32401592675961	37.0	37.0	37.0	35.0	37.0
5	36.3457177918172	37.0	37.0	37.0	35.0	37.0
6	33.210647710291866	35.0	32.0	35.0	31.0	35.0
7	35.46904496188549	35.0	35.0	37.0	35.0	37.0
8	36.463087795789434	37.0	37.0	37.0	35.0	37.0
9	38.63163871129438	39.0	39.0	39.0	38.0	39.0
10	38.089157106512566	39.0	38.0	39.0	37.0	39.0
11	37.50600327236272	39.0	37.0	39.0	35.0	39.0
12	36.98178743355969	39.0	37.0	39.0	34.0	39.0
13	36.8360656222218	39.0	37.0	39.0	33.0	39.0
14	37.72737364518509	40.0	37.0	40.0	33.0	40.0
15	37.82962374921974	40.0	37.0	40.0	33.0	40.0
16	37.883912696010746	40.0	37.0	40.0	33.0	40.0
17	37.73901725083701	40.0	37.0	40.0	33.0	40.0
18	37.68713883424442	40.0	37.0	40.0	33.0	40.0
19	37.66303530557815	40.0	37.0	40.0	33.0	40.0
20	37.57174253314923	40.0	37.0	40.0	33.0	40.0
21	33.32781082717007	38.0	33.0	40.0	10.0	40.0
22	33.946708249002214	38.0	33.0	40.0	16.0	40.0
23	35.88466635613899	38.0	34.0	40.0	30.0	40.0
24	36.69385261599864	38.0	35.0	40.0	31.0	40.0
25	36.96430133637241	39.0	35.0	40.0	32.0	40.0
26	37.01100284676642	39.0	35.0	40.0	32.0	40.0
27	36.94332534946186	39.0	35.0	40.0	32.0	40.0
28	36.80166739175667	38.0	35.0	40.0	32.0	40.0
29	36.68957005315225	38.0	35.0	40.0	31.0	40.0
30	36.615194142849035	38.0	35.0	40.0	31.0	40.0
31	36.42021014999906	38.0	35.0	40.0	31.0	40.0
32	36.39003418957005	38.0	35.0	40.0	31.0	40.0
33	36.015131130951254	38.0	35.0	40.0	30.0	40.0
34	35.69274961223447	38.0	34.0	40.0	29.0	40.0
35	35.59381620387009	38.0	34.0	40.0	29.0	40.0
36	35.53737267577127	38.0	34.0	40.0	29.0	40.0
37	35.372337067546866	37.0	34.0	40.0	29.0	40.0
38	35.28178140427866	37.0	34.0	40.0	29.0	40.0
39	35.01616733973935	37.0	33.0	39.0	28.0	40.0
40	30.271518622202887	35.0	28.0	39.0	8.0	40.0
41	30.944875820455103	35.0	27.0	39.0	12.0	40.0
42	32.52549794768003	35.0	29.0	38.0	22.0	40.0
43	33.36501503773621	35.0	31.0	38.0	25.0	40.0
44	34.406552646263265	35.0	33.0	39.0	27.0	40.0
45	34.77142049860972	35.0	33.0	39.0	28.0	40.0
46	34.848855973480624	35.0	34.0	39.0	29.0	40.0
47	34.78817657990807	35.0	34.0	39.0	29.0	40.0
48	34.586101088580776	35.0	33.0	39.0	29.0	40.0
49	34.41580404600223	35.0	33.0	39.0	28.0	40.0
50	34.35316927383812	35.0	33.0	39.0	28.0	40.0
51	34.18493696445798	35.0	33.0	38.0	28.0	40.0
52	33.721032260200126	35.0	33.0	37.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	5.0
15	20.0
16	68.0
17	113.0
18	274.0
19	528.0
20	928.0
21	1604.0
22	2786.0
23	4352.0
24	6364.0
25	9310.0
26	12386.0
27	16909.0
28	22764.0
29	31162.0
30	42807.0
31	60078.0
32	79991.0
33	111335.0
34	186080.0
35	199233.0
36	244009.0
37	285349.0
38	338533.0
39	34752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.3609553218454	0.6009774528533869	0.032983713847958084	0.005083511453269526
8	99.62807611553521	0.31943367317986643	0.02754553880492557	0.024944672479996974
9	98.6219546219759	0.919110692870789	0.19760673009627935	0.2613279550570299
10	66.44687375867744	21.73242523691528	4.20672394877712	7.6139770556301665
11	33.90394764219645	25.328950479505174	19.377045226700968	21.390056651597405
12	29.229777082111713	16.83262952314298	27.961854748709026	25.975738646036277
13	22.554121663797833	15.524630204097074	31.97658747422778	29.944660657877314
14	23.775346624548398	16.0686841507935	33.10359014129797	27.05237908336013
15	24.994975599145025	16.333736073543044	29.986924735657404	28.684363591654527
16	30.355479316019444	16.73403304518887	25.889141619535817	27.02134601925587
17	31.589294834206594	16.77416914143038	23.685557625740064	27.95097839862296
18	29.641364178031665	19.123106096430664	23.70908364386101	27.52644608167666
19	30.40891529687707	18.7518915391454	22.589824465167307	28.249368698810223
20	30.36753787807139	19.20095475438364	22.721700209960847	27.709807157584127
21	36.03429360470615	17.556379688652658	23.818674693097776	22.59065201354342
22	32.642231921614616	16.588620973386046	24.371595229538276	26.397551875461062
23	31.932786520892048	18.489263150925908	22.514517562940963	27.063432765241075
24	32.46430902075018	18.17059791552386	22.369933039514255	26.9951600242117
25	30.164670304726954	17.87333071670418	23.16993587682297	28.79206310174589
26	28.056727258970625	18.2373337809976	23.804488149507254	29.901450810524523
27	30.04142470728432	16.9733127470823	23.58908912932453	29.39617341630885
28	30.64021506800083	19.251612537121456	21.845326479656496	28.262845915221213
29	31.327139330773452	19.259178693703067	21.719598237085517	27.694083738437968
30	29.855817428641686	18.440024022547146	23.893213157546295	27.810945391264873
31	33.56293860063934	19.19137883746004	19.584050541925965	27.661632019974654
32	34.65920375659674	19.79732158057011	20.297988348118864	25.245486314714285
33	35.24469423269714	18.440910681521554	21.23589621124709	25.078498874534212
34	30.827063669207632	20.596496869502715	25.442856602417386	23.133582858872266
35	29.840389562486997	22.681386781924452	24.30976554372293	23.168458111865625
36	31.904295212514423	22.546141733028165	20.304608735127776	25.244954319329636
37	31.54478455369134	23.11638167476876	19.97577647681919	25.363057294720715
38	32.771920574271284	22.04393808992377	20.22947916469631	24.95466217110863
39	32.46330414057919	22.159026424801862	19.494911759698866	25.882757674920082
40	30.299324247640303	25.88334878090302	19.80813882005788	24.009188151398796
41	29.109959899370118	21.529380331775965	22.133431535740634	27.227228233113284
42	26.810971399928125	19.814936538861673	23.956284165925815	29.417807895284394
43	26.68057342009193	17.895201638072898	26.092304745871715	29.331920195963455
44	26.922572209506875	18.68551033726143	24.55743895057408	29.834478502657614
45	28.970458887396678	18.965635462575896	24.782413887680406	27.28149176234702
46	28.55822157489549	20.729141052074073	23.139434808103353	27.57320256492708
47	26.98493389070687	20.384703595815914	25.22000964684964	27.410352866627573
48	30.037878071386686	19.671238674409366	24.97789263623811	25.31299061796584
49	28.466541036941763	19.92937465715853	24.812205629220497	26.791878676679215
50	25.91355429663117	21.138127281669096	24.790571150244954	28.15774727145478
51	25.932174135093728	21.04384587739043	24.337665746117615	28.686314241398225
52	27.6151710897157	20.632968108650008	24.159210849868536	27.59264995176575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	3.5
12	6.0
13	320.5
14	873.0
15	1111.0
16	1343.0
17	1575.0
18	1462.0
19	1349.0
20	1274.5
21	1200.0
22	1743.0
23	2286.0
24	2602.5
25	2919.0
26	2806.0
27	2693.0
28	3574.0
29	4455.0
30	5156.5
31	5858.0
32	6479.0
33	7100.0
34	9650.0
35	12200.0
36	13212.5
37	14225.0
38	16479.0
39	20002.0
40	21271.0
41	22496.5
42	23722.0
43	26918.0
44	30114.0
45	32714.0
46	35314.0
47	34031.5
48	32749.0
49	36875.5
50	41002.0
51	45574.5
52	50147.0
53	56281.0
54	62415.0
55	68525.5
56	74636.0
57	84354.0
58	94072.0
59	113804.0
60	133536.0
61	155522.5
62	177509.0
63	172350.5
64	172898.0
65	178604.0
66	162000.5
67	145397.0
68	127749.5
69	110102.0
70	91517.0
71	72932.0
72	63120.5
73	53309.0
74	50184.0
75	47059.0
76	41869.5
77	36680.0
78	27228.0
79	17776.0
80	12220.0
81	6664.0
82	4168.0
83	1672.0
84	1280.0
85	888.0
86	674.0
87	460.0
88	280.0
89	70.0
90	40.0
91	34.0
92	28.0
93	16.0
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1691744.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.079858981165685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.74363653581915	6.605019615224098
2	9.2879124182333	1.6866586997395188
3	3.8744794140074355	1.055391801138509
4	2.0178163557567608	0.7328595184064414
5	1.1494899569429702	0.5218603354654192
6	0.739094294432846	0.40265231803406354
7	0.4967895227440151	0.31575451668853816
8	0.3582737138245347	0.2602459838548229
9	0.24381239102276658	0.19924039155127926
>10	3.6353314708728246	8.904695591178232
>50	2.6287128635624035	17.475768578428436
>100	2.649652823784238	42.54309593479408
>500	0.1159176369422966	7.253056116816108
>1k	0.05459346772121066	9.062907957710939
>5k	0.004487134333250192	2.9807926409695136
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	9827	0.580879849433484	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	9160	0.5414530803714982	No Hit
GTCGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	8139	0.4811011595134962	No Hit
GTCGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5894	0.348397866343844	No Hit
GTCGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5603	0.331196682240339	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5274	0.31174929540166835	No Hit
GTCGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4752	0.2808935630922882	No Hit
GTCGGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	4565	0.26983988121134167	No Hit
GTCGGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3546	0.20960618154992716	No Hit
GTCGGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	3530	0.20866041197722587	No Hit
GTCGGTGGGTCATTCAGATTGCCATCATCTCTATCTTCAAGTCCTATCCAAC	3527	0.20848308018234438	No Hit
GTCGGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	3478	0.2055866608659466	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	3454	0.20416800650689465	No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2892	0.17094785026576126	No Hit
GTCGGTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	2750	0.16255414530803716	No Hit
GTCGGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2730	0.16137193334216052	No Hit
GTCGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2669	0.15776618684623678	No Hit
GTCGGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2582	0.15262356479467343	No Hit
GTCGGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	2521	0.1490178182987497	No Hit
GTCGGTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	2485	0.14688983676017175	No Hit
GTCGGTGGGCTGTAGGTGTCTGTGTGGGTGTGGGTGTGCTATAGGTATCTGT	2448	0.1447027446233	No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2412	0.14257476308472203	No Hit
GTCGGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	2335	0.138023247016097	No Hit
GTCGGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2317	0.13695925624680805	No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2314	0.13678192445192652	No Hit
GTCGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	2178	0.12874288308396542	No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2115	0.12501891539145402	No Hit
GTCGGTGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	1999	0.11816208598936954	No Hit
GTCGGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1903	0.1124874685531617	No Hit
GTCGGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1787	0.10563063915107723	No Hit
GTCGGTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	1729	0.10220222445003499	No Hit
GTCGGTGGGGTTCCCCTGTTTTGAGTATTTCTGTCCCTGGAATAACCTTTTA	1718	0.10155200786880285	No Hit
GTCGGTGGGATCTTACAGTTACCTCATTTTTCCCATGTATGTATTTGAGAAA	1701	0.10054712769780771	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.911059829383169E-5	0.0	0.0	0.0	0.0
8	1.1822119658766338E-4	0.0	0.0	0.0	0.0
9	2.3644239317532675E-4	0.0	0.0	0.0	0.0
10	0.0024235345300470993	0.0	0.0	0.0	0.0
11	0.005319953846444852	0.0	0.0	0.0	0.0
12	0.007920820171373447	0.0	0.0	0.0	0.0
13	0.010167022906539052	0.0	0.0	0.0	0.0
14	0.01288611042805531	0.0	0.0	0.0	0.0
15	0.017555847693268012	0.0	0.0	0.0	0.0
16	0.02305313333459436	0.0	0.0	0.0	0.0
17	0.02577222085611062	0.0	0.0	0.0	0.0
18	0.03008729453156033	0.0	0.0	0.0	0.0
19	0.03475703179677304	0.0	0.0	0.0	0.0
20	0.03765345111317079	0.0	0.0	0.0	0.0
21	0.04190941419032667	0.0	0.0	0.0	0.0
22	0.046047156070894886	0.0	0.0	0.0	0.0
23	0.048825354190704974	0.0	0.0	0.0	0.0
24	0.05343598085762385	0.0	0.0	0.0	0.0
25	0.05751461213989824	0.0	0.0	0.0	0.0
26	0.060056367866533	0.0	0.0	0.0	0.0
27	0.06271634478975542	0.0	0.0	0.0	0.0
28	0.0673269714566743	0.0	0.0	0.0	0.0
29	0.07164204513212401	0.0	0.0	0.0	0.0
30	0.07583889761098606	0.0	0.0	0.0	0.0
31	0.08098151966254942	0.0	0.0	0.0	0.0
32	0.09085298957761931	0.0	0.0	0.0	0.0
33	0.09587739043259501	0.0	0.0	0.0	0.0
34	0.10261599863809182	0.0	0.0	0.0	0.0
35	0.1102412658179961	0.0	0.0	0.0	0.0
36	0.11757098000643124	0.0	0.0	0.0	0.0
37	0.12360026103240207	0.0	0.0	0.0	0.0
38	0.12880199368225925	0.0	0.0	0.0	0.0
39	0.13477216410993625	0.0	0.0	0.0	0.0
40	0.14340231146083568	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAC	55	1.8189894E-12	46.000004	40
ACGACAC	35	1.0199619E-7	46.000004	38
GATAACG	25	3.4178447E-5	46.0	19
TTTACGG	25	3.4178447E-5	46.0	13
CAACGAA	30	1.8619285E-6	46.0	20
ACGCATC	20	6.3123036E-4	46.0	40
CGTGCAA	30	1.8619285E-6	46.0	40
TACGAAT	20	6.3123036E-4	46.0	23
CGTACTC	25	3.4178447E-5	46.0	21
CGTAAGA	20	6.3123036E-4	46.0	17
CGTAACC	20	6.3123036E-4	46.0	27
ATAACGT	20	6.3123036E-4	46.0	15
ATAACGC	40	5.6134013E-9	46.0	39
ATAACGA	25	3.4178447E-5	46.0	20
CGCACTA	30	1.8619285E-6	46.0	33
ACGTAAT	30	1.8619285E-6	46.0	20
TATACGT	20	6.3123036E-4	46.0	34
TCGAAAC	20	6.3123036E-4	46.0	30
ATCGATT	20	6.3123036E-4	46.0	12
GTACGAC	25	3.4178447E-5	46.0	10
>>END_MODULE