FastQCFastQC Report
Fri 17 Jun 2016
SRR1527224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80946
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGATTGTTGTTCCTCCTATGGGGCTTCAAACCCCTTCAGTTCCTT12141.4997652756158426No Hit
CTGTGTGGGTCCTGTTGAAAAGCCATGTTTAAGATTTAATTTGCCAAAATAA7800.9636053665406566No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGG5820.7189978504187977No Hit
CTGTGTGGGCATGGCCTTTCCCTTCTGGTCCCCCCTCATCCACATATTGTGC4710.5818693944110889No Hit
CTGTGTGGGGAGTGCAGTGCTCTTGTGGGATTTCCACATCTTTTAGATCATA3600.4447409384033801No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAA3450.4262100659699059No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAA3320.41014997652756163No Hit
CTGTGTGGGATGGCTCGTGTGCAGCGTAGATCTATCACATGTGGAAGTGTGT3090.3817359721295679No Hit
CTGTGTTTGTGGGAGATGGTTCTTTTTTTGATTCTAATAAATGAGCTGTGTT3030.3743236231561782No Hit
CTGTGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG3030.3743236231561782No Hit
CTGTGTGGGAGGATTTGTTTTTTCCCACATAAGTTCAAGGCTAGCTTCCACC2940.3632050996960937No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGACAGCTGCGCAGTTCTGTCAG2890.35702814221826895No Hit
CTGTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT2880.355792750722704No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGTGCAGTCCTCTGTGTGGGGCTCTCTT2880.355792750722704No Hit
CTGTGTGGGCTCTTCTTATTTGGCCAGCACTCCCCATCCACCTGTCTTAACA2820.3483804017493144No Hit
CTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2720.3360264867936649No Hit
CTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.3187310058557557No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGA2500.30884787389123614No Hit
CTGTGTGGGCAGGCCGTACAGCGAGTATTCGAGGAAGTTGGCGTCGGTGTTT2420.29896474192671657No Hit
CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.28661082697106716No Hit
CTGTGTGGGATGAGCCACGGAGGCAATGGTATTTTTGCTACACTAATCAGAA2290.2829046524843723No Hit
CTGTGTGGGGGCTGGGTCGGAAAAGCTGCCTCACTGAGCTGTCCCGCTCCTC2290.2829046524843723No Hit
CTGTGTGGGGATGATGAAGATGCCACAGTTAACAAGATTGCGCTGGAGCTGT2280.28166926098880735No Hit
CTGTGTGGGGTCACTGAAGTGGTGGGTGATAGACATCACCCTGGAGATGTGT2270.2804338694932424No Hit
CTGTGTGGGCCTCTCAGCCTGGGGTCCCCACCCCTAGAAGACAGAGGAACTG2200.27178612902428784No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGAAGAAAAAAAGTACTTAAAAAGT2200.27178612902428784No Hit
CTGTGTGGGATCAAAACTGCTTTCATCCCGTTTTTATTAATATTTATTAGTG2050.2532552565908136No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2010.24831369060855385No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAAAA2000.2470782991129889No Hit
CTGTGTGGGAGTGCATTGCTCTGTGTGGGGCTTGCACTGGAACTGGGACAGG1960.24213673313072914No Hit
CTGTGTGGGGTGCAGTGCCTGTGTGGGGTGCAGTGCTCTGTGTGGGGAAAAA1960.24213673313072914No Hit
CTGTGTGGGGAAGGACTGTGGCCTCTTCTGAGGGGCAGTGGACCTGGCAGGA1910.2359597756529044No Hit
CTGTGTGGGAGTAGGGGTGAGAGGTGGGCTGCAGACAGTGAGCCTGGTGGCA1900.23472438415733946No Hit
CTGTGTGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTC1890.23348899266177453No Hit
CTGTGTGGGGTGCAGTGCTCTGCATGAGAACAACCCACGTGGGCATGGCCAC1890.23348899266177453No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTGTGTGGGAAG1870.23101820967064463No Hit
CTGTGTGGGGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACAT1850.22854742667951472No Hit
CTGTGTGGGATCAGGCTTTCCAATGACTTTCAGTGGTGGAAAATTTACTGAG1830.22607664368838484No Hit
CTGTGTGGGTGTTTTGTTTGTGTGTTTTTCAAAGATACCATGATCATTCATC1780.21989968621056014No Hit
CTGTGTGGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGG1760.2174289032194302No Hit
CTGTGTGGGAAGTGCTGTGATTAAAGTCATGCGCCACCCCTGCCCGGCCATA1640.20260420527265088No Hit
CTGTGTGGGATTTGAATGCTCCAATTTACTAGACTGCACCTTACAGTAAACA1640.20260420527265088No Hit
CTGTGTGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCG1630.20136881377708593No Hit
CTGTGTGGGAAACTAACCGACTCACTGGGAGACGATGCCAGGGGGTCTAGAA1630.20136881377708593No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGCAGTGCTCTGTGTGGGGAAAAAAA1610.19889803078595608No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGCAGTGATCTGTGTGGGCAGTGCTCTGT1580.19519185629926122No Hit
CTGTGTGGGGTGCTCTGTGTGGGCATACTACTTACAATAAGCTATTGTGCTA1570.19395646480369627No Hit
CTGTGTGGGGCTTTCTGCTGGGTGCCGGCTGCCTGCTGTCGAGATGGGCAAG1570.19395646480369627No Hit
CTGTGTGGGGGGTGGCCGAGTCGCCGCAGCTGTAGCAGCCGCCGCGAAGATG1570.19395646480369627No Hit
CTGTGTGGGGTTGTCCTCCTGCTGGCCAAGCGGCCTCTTAGTAGTGGTGGGT1570.19395646480369627No Hit
CTGTGTGGGGAGCTGGGGCTTCGAGTCCTGGGCTGCAAAGCCAGTTCTGTGC1560.19272107330813135No Hit
CTGTGTGGGGGGCATTTGCGTCTCTATGGTTGTCAGAGGTGGGCGGCATTCT1560.19272107330813135No Hit
CTGTGTGGTGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGC1550.1914856818125664No Hit
CTGTGTGGGATGTCTACAGAATAAAGTCATCATATCTCAAAAAAAAAAAAAA1520.18777950732587156No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGCAAAAAAAAAAAAAAAAAA1490.18407333283917673No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGATGCTGAGTGAACTTAGCTGTG1470.18160254984804686No Hit
CTGTGTGGGATTAACCTAAATGTAGCTCCTTAGCGCTAATCTAGGAACCGCC1450.17913176685691695No Hit
CTGTGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1440.177896375361352No Hit
CTGTGTTTGAAGCTTGCCGGTAGTGTTGCTTTGAGTTACAGTATAGCCTGCG1440.177896375361352No Hit
CTGTGTGGGGTGCTCTGTGAGGACTGCTATCAGTTCAAGGCTAGCCTGCCTT1440.177896375361352No Hit
CTGTGTGGATTTTTCTTGCAGACAATAGATCCAGACTGCAGACTGGCCCTCT1410.1741902008746572No Hit
CTGTGTGGGAGGACGACTGGGGCGCCTGGCAGAAGTTCACGGCTAGTGCGGG1410.1741902008746572No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAGAGTGCAGTGCTCTGTGTGGG1390.1717194178835273No Hit
CTGTGTGGGCCACCGGGTCCGGCTGGTCGGTGGCCTGCAAGGACATTGCCGA1350.1667778519012675No Hit
CTGTGTGGGATGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.16554246040570259No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGTATCGCTCTCTGTGGGGAAAAAAA1340.16554246040570259No Hit
CTGTGTGGCGTGGGGGCCTCTATATATTGTACATGTCACTGAGTGCCTTCAA1340.16554246040570259No Hit
CTGTGTGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA1330.1643070689101376No Hit
CTGTGTGGGATTTCAATGATGTGTCCCTGGAATCCTGGGAGGACAGAACCCT1320.16307167741457268No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA1300.1606008944234428No Hit
CTGTGTGGGATTGTTATTCCTCCTATGGGGCTTCAAACCCCTTCAGTTCCTT1290.15936550292787785No Hit
CTGTGTGGGATGTTTCAAAATTAAGAATGGGTTTAAACTTGCTGAATGTAAA1280.1581301114323129No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGAATAAGAGAGGTTCAATAAAAATGC1280.1581301114323129No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA1280.1581301114323129No Hit
CTGTGTGGGATGCCGGTCGCCAGAAGCTGGGTTTGTCGCAAAACCTATGTGA1270.15689471993674795No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGTCAGATCACCTTGTGAGGCCTAT1250.15442393694561807No Hit
CTGTGTGGGGTTTTTATTGTTTTCAATTGTGTGTAGGTGTATGTGCCTGCTT1240.15318854545005312No Hit
CTGTGTGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTC1240.15318854545005312No Hit
CTGTGTGGGAGCTGGGACTACACAAAGGAACCCTGTCTTGAAAAATCAAAAA1230.1519531539544882No Hit
CTGTGTGGGAGTATGTTTCACTTTAAGGGGGAAGGCAGGAGGGTTTGGAGGA1230.1519531539544882No Hit
CTGTGTGGGCAGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGG1210.14948237096335829No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.14948237096335829No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGTAAAAAAAAAAAAAAAAAAA1200.14824697946779333No Hit
CTGTGTGGGAGGCTCTGTGTGGGAGTGCAGTGCTCTGTGTGGGTTATGGCCA1200.14824697946779333No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGAGCTCTGTGTGGGA1200.14824697946779333No Hit
CTGTGTGGGACACAGCCCTGCAGTCCTGTGCGGCACGTCCAGCATGTTGTTT1190.1470115879722284No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGCAAAAAAAAAAAAAAAAAAAA1180.14577619647666346No Hit
CTGTGTGGGAGGGGTGGGCAACATAGGCAGCTTCTTTAGCAGCCTCCCAGCA1180.14577619647666346No Hit
CTGTGTGGGAGCGGCTGCCCACATCCACTTATTCCCTCTGACCACTTCTCAC1160.14330541348553358No Hit
CTGTGTGGGAATCAAATGATGCCAGTGGGGATGAAAGCACATACGTTCCTAG1150.14207002198996863No Hit
CTGTGTGGGACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTC1130.13959923899883872No Hit
CTGTGTGGGATCGAAACGACCAAGAAGAAACTGGAGATCATACAATCCCAGA1130.13959923899883872No Hit
CTGTGTGGGGTGCTTGGGGTGATCTTCCTCGGGCCTGGCTTTTTCATCCGTC1110.13712845600770884No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGAATTCCTTCGATTCTGTTT1100.13589306451214392No Hit
CTGTGTGGGACGCAGAGTGCAGTCCTCTGTGTGGGGGTTTTGGTTTTGAGGC1070.13218689002544906No Hit
CTGTGTGGGGTTACCAGAAATCGACTGAGCTGCTCATCCGGAAGCTGCCATT1060.1309514985298841No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAA1060.1309514985298841No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGATAGATTTTCTCTCCTGGGAAT1050.12971610703431918No Hit
CTGTGTGGGGAGAGACAATGGCAGGGCTGTGGAAGACCTTTGTATTGGTGGT1030.12724532404318928No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGAGAATGAAAAAAAAAAAAAAAAA1030.12724532404318928No Hit
CTGTGTGGGATTTCATTCTCAAAAAAATTTTTTTTCCTCTTCTTCCTGTTAT1020.12600993254762433No Hit
CTGTGTGGGGGGGGTGGGGGAGATGCAAAACTTTTAAGGACCATAGGGCTGA1020.12600993254762433No Hit
CTGTGTGGGAGCTAGAGATAGCAACTTACATACAGAGTCTCTGATTCCTCAA1010.1247745410520594No Hit
CTGTGTGGGGGGTGTAGAGAGCCCGAACCAGATCGGGCTCTCTACACCCGCA1010.1247745410520594No Hit
CTGTGTGGGGAGTGCAGTTCTCTGTGTGGGAAGCAGTGGTATCGTGCAGTGC980.12106836656536457No Hit
CTGTGTGGGCAGAGTGCAGTGCTCTGTGTGGGCAGTGCTCTGTGTGGGAGAG970.1198329750697996No Hit
CTGTGTGGGGCACCTGGGATTTATTGAAATAAGAGAGAAGCTGGAAGAATTA960.11859758357423468No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGGTGTTCCTGGGAAGGGCAGGAAG960.11859758357423468No Hit
CTGTGTGGGGGCTTCTCCATACTCCAGGGCCCAGCCCCAGGGGAGCACAGAG950.11736219207866973No Hit
CTGTGTGGGGTCTCTGAAAAGGAAAAGTTCGAAGATGTGCCCCCTCACATCA940.11612680058310479No Hit
CTGTGTGGGCAGAGTGCAGTGCTCTGTGTGGGGTGCAGTGCTCTGTGTGGGC940.11612680058310479No Hit
CTGTGTGGGTCCTGTAGAAAAGCCATGTTTAAGATTTAATTTGCCAAAATAA930.11489140908753984No Hit
CTGTGTGGGCAGAGTGCAGTGCTCTGTGTGGGAAGCAGTGGTATTGTGTGGG930.11489140908753984No Hit
CTGTGTGGGGATGCAGCCCCTCCTCTACAATTACAGGGAGAAAGAGGTCACG920.1136560175919749No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA920.1136560175919749No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGCTCT920.1136560175919749No Hit
CTGTGTGGGGTGTGGGGAAGCAGAATGCTGGTGGCGAGGTGGGAAGAACGTG910.11242062609640995No Hit
CTGTGTGGGGGAAGCCTCCTCATTCTCCCAGAGCCAAAGTCCTGCCCACATC900.11118523460084502No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGTGCTCTGTGTGGGAAAAAAA890.10994984310528007No Hit
CTGTGTGGGGTGTCCCCTGAATCTGGAAAATGAAATAAAAGTCAATGTTAAA880.1087144516097151No Hit
CTGTGTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTTCTGGCAGCATTTGA860.10624366861858522No Hit
CTGTGTGGGAGTCGTGTGAGCTGCAGGGCTTTGTGCAGTCCCAGCACCCAGT850.10500827712302029No Hit
CTGTGTGGGAGAAGCTCCCTCAGCGAGGACAGCAAGGGACTAGCCAGGAGGG850.10500827712302029No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGGACAATCCGCAGCGGCTTCTGAG850.10500827712302029No Hit
CTGTGTGGGAATGAATAATGTTGTTATGAATATTCATACACAAAGCTTAAAA840.10377288562745533No Hit
CTGTGTGGGGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAG840.10377288562745533No Hit
CTGTGTGGGGTTGAAACAAAACAAAACTGAGTTCTCTGAAAATGGTTTAAAT840.10377288562745533No Hit
CTGTGTGGGGATGAAAGGCTAGGACTCTTTCAGCTCATGGCTTGTGTGGAAG840.10377288562745533No Hit
CTGTGTGGGGCTGGTTGAACTGGCGCTGGAAGCCGGGGTGCCAGCCGGTGTG840.10377288562745533No Hit
CTGTGTGGGGTGCTCTGTGTGGGGCGTCCCCACCGACCAATGCATTGACAAC830.10253749413189041No Hit
CTGTGTGGGGCTTTGCCCCATCTACTGCGAGGATGAAGACCATTCTCAGCAA830.10253749413189041No Hit
CTGTGTGGGACGTAGACTTCCTGCGAGTGTTTAGTGTAATTTTCAGATACTA830.10253749413189041No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGACGCAGAGTGCAGTGCTCTGTG820.10130210263632544No Hit
CTGTGTGGGACATCAGTTCACTCATAATCTTCAAACCACATTGGTATTACCT820.10130210263632544No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAA810.10006671114076052No Hit
CTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCCGAGT810.10006671114076052No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGGAAAAAAAAAAAAAAAAAA810.10006671114076052No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAA206.2765536E-446.00000442
ATGGGTT206.2765536E-446.00000427
TGAGAAC206.2765536E-446.00000425
CTTTCCC551.8189894E-1246.00000416
AGCCTGG206.2765536E-446.00000416
CGCGAAG206.2765536E-446.00000443
CTTTTAT206.2765536E-446.00000426
TTTGAGT206.2765536E-446.00000430
TGATGAA405.500624E-946.00000412
CAAGATT405.500624E-946.00000433
TAGAACA206.2765536E-446.00000445
AGCCTAT206.2765536E-446.00000428
ACAGTAT206.2765536E-446.00000438
ACAGTAA206.2765536E-446.00000443
TTTGACA206.2765536E-446.00000431
TTTGAAT206.2765536E-446.00000411
TTCACTA206.2765536E-446.00000440
AAACTTG206.2765536E-446.00000435
AGGGGGT206.2765536E-446.00000440
ACTGCGA206.2765536E-446.00000424
Produced by FastQC (version 0.11.5)