FastQCFastQC Report
Fri 17 Jun 2016
SRR1527223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79855
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGATTGTTGTTCCTCCTATGGGGCTTCAAACCCCTTCAGTTCCTT12011.5039759564210131No Hit
CTGTGTGGGTCCTGTTGAAAAGCCATGTTTAAGATTTAATTTGCCAAAATAA7630.9554818107820424No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGG4860.608603093106255No Hit
CTGTGTGGGCATGGCCTTTCCCTTCTGGTCCCCCCTCATCCACATATTGTGC4540.5685304614613987No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAA3530.4420512178323211No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAA3450.432033059921107No Hit
CTGTGTGGGCTCTTCTTATTTGGCCAGCACTCCCCATCCACCTGTCTTAACA3390.42451944148769644No Hit
CTGTGTGGGATGGCTCGTGTGCAGCGTAGATCTATCACATGTGGAAGTGTGT3380.4232671717487947No Hit
CTGTGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG3110.38945588879844717No Hit
CTGTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT3090.3869513493206437No Hit
CTGTGTGGGGAGTGCAGTGCTCTTGTGGGATTTCCACATCTTTTAGATCATA3020.37818546114833135No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGTGCAGTCCTCTGTGTGGGGCTCTCTT2990.3744286519316261No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGACAGCTGCGCAGTTCTGTCAG2830.3543923361091979No Hit
CTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.34186963872018034No Hit
CTGTGTTTGTGGGAGATGGTTCTTTTTTTGATTCTAATAAATGAGCTGTGTT2690.3368605597645733No Hit
CTGTGTGGGAGGATTTGTTTTTTCCCACATAAGTTCAAGGCTAGCTTCCACC2600.3255901321144575No Hit
CTGTGTGGGCAGGCCGTACAGCGAGTATTCGAGGAAGTTGGCGTCGGTGTTT2440.3055538162920293No Hit
CTGTGTGGGATGAGCCACGGAGGCAATGGTATTTTTGCTACACTAATCAGAA2230.27925615177509233No Hit
CTGTGTGGGGTCACTGAAGTGGTGGGTGATAGACATCACCCTGGAGATGTGT2210.27675161229728884No Hit
CTGTGTGGGGGCTGGGTCGGAAAAGCTGCCTCACTGAGCTGTCCCGCTCCTC2200.2754993425583871No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAAAA2160.27049026360278No Hit
CTGTGTGGGCCTCTCAGCCTGGGGTCCCCACCCCTAGAAGACAGAGGAACTG2120.265481184647173No Hit
CTGTGTGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTC2110.2642289149082712No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGA2090.2617243754304677No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2090.2617243754304677No Hit
CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.2617243754304677No Hit
CTGTGTGGGAGTGCATTGCTCTGTGTGGGGCTTGCACTGGAACTGGGACAGG2050.2567152964748607No Hit
CTGTGTGGGATCAAAACTGCTTTCATCCCGTTTTTATTAATATTTATTAGTG2040.25546302673595894No Hit
CTGTGTGGGGATGATGAAGATGCCACAGTTAACAAGATTGCGCTGGAGCTGT1960.24544486882474484No Hit
CTGTGTGGGGAAGGACTGTGGCCTCTTCTGAGGGGCAGTGGACCTGGCAGGA1960.24544486882474484No Hit
CTGTGTGGGGTGCAGTGCTCTGCATGAGAACAACCCACGTGGGCATGGCCAC1940.24294032934694135No Hit
CTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.23918352013023603No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGAAGAAAAAAAGTACTTAAAAAGT1870.23417444117462902No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTGTGTGGGAAG1860.23292217143572724No Hit
CTGTGTGGGATCAGGCTTTCCAATGACTTTCAGTGGTGGAAAATTTACTGAG1840.23041763195792375No Hit
CTGTGTGGGAGTAGGGGTGAGAGGTGGGCTGCAGACAGTGAGCCTGGTGGCA1810.22666082274121843No Hit
CTGTGTGGTGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGC1750.21914720430780793No Hit
CTGTGTGGGGTGCAGTGCCTGTGTGGGGTGCAGTGCTCTGTGTGGGGAAAAA1700.21288585561329912No Hit
CTGTGTGGGATTAACCTAAATGTAGCTCCTTAGCGCTAATCTAGGAACCGCC1690.21163358587439735No Hit
CTGTGTGGGCCACCGGGTCCGGCTGGTCGGTGGCCTGCAAGGACATTGCCGA1680.21038131613549557No Hit
CTGTGTGGGTGTTTTGTTTGTGTGTTTTTCAAAGATACCATGATCATTCATC1670.20912904639659383No Hit
CTGTGTGGGGTTGTCCTCCTGCTGGCCAAGCGGCCTCTTAGTAGTGGTGGGT1670.20912904639659383No Hit
CTGTGTGGGGTGCTCTGTGAGGACTGCTATCAGTTCAAGGCTAGCCTGCCTT1620.20286769770208501No Hit
CTGTGTGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCG1610.20161542796318324No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGCAGTGCTCTGTGTGGGGAAAAAAA1540.19284953979087097No Hit
CTGTGTGGGAAGTGCTGTGATTAAAGTCATGCGCCACCCCTGCCCGGCCATA1540.19284953979087097No Hit
CTGTGTGGGATGCCGGTCGCCAGAAGCTGGGTTTGTCGCAAAACCTATGTGA1490.18658819109636215No Hit
CTGTGTGGGGAGCTGGGGCTTCGAGTCCTGGGCTGCAAAGCCAGTTCTGTGC1480.18533592135746038No Hit
CTGTGTGGGGTGCTCTGTGTGGGCATACTACTTACAATAAGCTATTGTGCTA1460.1828313818796569No Hit
CTGTGTGGGGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACAT1440.18032684240185337No Hit
CTGTGTGGGAGGCTCTGTGTGGGAGTGCAGTGCTCTGTGTGGGTTATGGCCA1400.1753177634462463No Hit
CTGTGTGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA1390.17406549370734456No Hit
CTGTGTGGGAAACTAACCGACTCACTGGGAGACGATGCCAGGGGGTCTAGAA1390.17406549370734456No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGCAGTGATCTGTGTGGGCAGTGCTCTGT1380.1728132239684428No Hit
CTGTGTGGGGGGTGGCCGAGTCGCCGCAGCTGTAGCAGCCGCCGCGAAGATG1380.1728132239684428No Hit
CTGTGTGGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGG1370.17156095422954104No Hit
CTGTGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1370.17156095422954104No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGTATCGCTCTCTGTGGGGAAAAAAA1360.1703086844906393No Hit
CTGTGTTTGAAGCTTGCCGGTAGTGTTGCTTTGAGTTACAGTATAGCCTGCG1360.1703086844906393No Hit
CTGTGTGGGGGGCATTTGCGTCTCTATGGTTGTCAGAGGTGGGCGGCATTCT1360.1703086844906393No Hit
CTGTGTGGGACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTC1350.16905641475173752No Hit
CTGTGTGGGATTTGAATGCTCCAATTTACTAGACTGCACCTTACAGTAAACA1330.166551875273934No Hit
CTGTGTGGGGCTTTCTGCTGGGTGCCGGCTGCCTGCTGTCGAGATGGGCAAG1320.16529960553503226No Hit
CTGTGTGGGAGCTGGGACTACACAAAGGAACCCTGTCTTGAAAAATCAAAAA1320.16529960553503226No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA1310.16404733579613048No Hit
CTGTGTGGGATGTCTACAGAATAAAGTCATCATATCTCAAAAAAAAAAAAAA1250.15653371736271993No Hit
CTGTGTGGGAGCGGCTGCCCACATCCACTTATTCCCTCTGACCACTTCTCAC1230.1540291778849164No Hit
CTGTGTGGGGTGCTTGGGGTGATCTTCCTCGGGCCTGGCTTTTTCATCCGTC1230.1540291778849164No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAGAGTGCAGTGCTCTGTGTGGG1230.1540291778849164No Hit
CTGTGTGGGGGCTTCTCCATACTCCAGGGCCCAGCCCCAGGGGAGCACAGAG1230.1540291778849164No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA1200.15027236866821114No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGATGCTGAGTGAACTTAGCTGTG1190.14902009892930937No Hit
CTGTGTGGGCAGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGG1190.14902009892930937No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGAGCTCTGTGTGGGA1190.14902009892930937No Hit
CTGTGTGGATTTTTCTTGCAGACAATAGATCCAGACTGCAGACTGGCCCTCT1180.1477678291904076No Hit
CTGTGTGGGATGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.1477678291904076No Hit
CTGTGTGGGATGTTTCAAAATTAAGAATGGGTTTAAACTTGCTGAATGTAAA1180.1477678291904076No Hit
CTGTGTGGGATTTCAATGATGTGTCCCTGGAATCCTGGGAGGACAGAACCCT1170.14651555945150585No Hit
CTGTGTGGGAGTATGTTTCACTTTAAGGGGGAAGGCAGGAGGGTTTGGAGGA1150.14401101997370236No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGAATAAGAGAGGTTCAATAAAAATGC1150.14401101997370236No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGATAGATTTTCTCTCCTGGGAAT1140.14275875023480059No Hit
CTGTGTGGGAGGACGACTGGGGCGCCTGGCAGAAGTTCACGGCTAGTGCGGG1130.1415064804958988No Hit
CTGTGTGGCGTGGGGGCCTCTATATATTGTACATGTCACTGAGTGCCTTCAA1130.1415064804958988No Hit
CTGTGTGGGACGCAGAGTGCAGTCCTCTGTGTGGGGGTTTTGGTTTTGAGGC1120.14025421075699707No Hit
CTGTGTGGGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.13774967127919355No Hit
CTGTGTGGGGTTTTTATTGTTTTCAATTGTGTGTAGGTGTATGTGCCTGCTT1090.13649740154029177No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGTCAGATCACCTTGTGAGGCCTAT1090.13649740154029177No Hit
CTGTGTGGGATCGAAACGACCAAGAAGAAACTGGAGATCATACAATCCCAGA1070.13399286206248825No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGGGTGTTCCTGGGAAGGGCAGGAAG1040.130236052845783No Hit
CTGTGTGGGACACAGCCCTGCAGTCCTGTGCGGCACGTCCAGCATGTTGTTT1030.12898378310688122No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGCTCT1030.12898378310688122No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGTAAAAAAAAAAAAAAAAAAA1020.12773151336797947No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGCAAAAAAAAAAAAAAAAAA1020.12773151336797947No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAA1010.1264792436290777No Hit
CTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCCGAGT1000.12522697389017595No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGGGGGGGCAAAAAAAAAAAAAAAAAAAA1000.12522697389017595No Hit
CTGTGTGGGGCTGGTTGAACTGGCGCTGGAAGCCGGGGTGCCAGCCGGTGTG1000.12522697389017595No Hit
CTGTGTGGGAGGGGTGGGCAACATAGGCAGCTTCTTTAGCAGCCTCCCAGCA990.12397470415127418No Hit
CTGTGTGGGAATCAAATGATGCCAGTGGGGATGAAAGCACATACGTTCCTAG990.12397470415127418No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGACGCAGAGTGCAGTGCTCTGTG980.12272243441237242No Hit
CTGTGTGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTC980.12272243441237242No Hit
CTGTGTGGGAAGAGGAGGAGGAACCAGAAAGAATACAAGGAGACAACGCATA970.12147016467347067No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGAATTCCTTCGATTCTGTTT960.1202178949345689No Hit
CTGTGTGGGGTTACCAGAAATCGACTGAGCTGCTCATCCGGAAGCTGCCATT960.1202178949345689No Hit
CTGTGTGGGGCACCTGGGATTTATTGAAATAAGAGAGAAGCTGGAAGAATTA960.1202178949345689No Hit
CTGTGTGGGGAGAGACAATGGCAGGGCTGTGGAAGACCTTTGTATTGGTGGT960.1202178949345689No Hit
CTGTGTGGGGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA960.1202178949345689No Hit
CTGTGTGGGGGGTGTAGAGAGCCCGAACCAGATCGGGCTCTCTACACCCGCA960.1202178949345689No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGCAAAAAAAAAAAAAAAAAAAA950.11896562519566715No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGTGCTCTGTGTGGGAAAAAAA920.11520881597896188No Hit
CTGTGTGGGGTGGGTTAGCTTTGAAGTTGAAATTCTCTTCTGTCTGCCTCCC910.1139565462400601No Hit
CTGTGTGGGATTTCATTCTCAAAAAAATTTTTTTTCCTCTTCTTCCTGTTAT910.1139565462400601No Hit
CTGTGTGGGGTCTCTGAAAAGGAAAAGTTCGAAGATGTGCCCCCTCACATCA900.11270427650115836No Hit
CTGTGTGGGCAGAGTGCAGTGCTCTGTGTGGGCAGTGCTCTGTGTGGGAGAG890.11145200676225658No Hit
CTGTGTGGGGTGTGGGGAAGCAGAATGCTGGTGGCGAGGTGGGAAGAACGTG880.11019973702335482No Hit
CTGTGTGGGGAGCCTGAGCCTTCTCAATGTGAACGGCAACTCAGGGACGAAG880.11019973702335482No Hit
CTGTGTGGGACTTTTTGAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.11019973702335482No Hit
CTGTGTGGGGGGGGTGGGGGAGATGCAAAACTTTTAAGGACCATAGGGCTGA880.11019973702335482No Hit
CTGTGTGGGGATGGTGCTCTGTGTGGGGGGAATTCGTGGAGAAAGAAATGGC870.10894746728445308No Hit
CTGTGTGGGAGAAGCTCCCTCAGCGAGGACAGCAAGGGACTAGCCAGGAGGG850.10644292780664956No Hit
CTGTGTGGGAAGGAAATCACCATGCCCTCTACAAGAATGACTTCAAGAAAAT850.10644292780664956No Hit
CTGTGTGGGGCTTTCCTCTGGCGGCAGCCATCAGGTGAGCCAAGATGGGTGC840.10519065806774779No Hit
CTGTGTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCA820.10268611858994428No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGGAAAAAAAAAAAAAAAAAA820.10268611858994428No Hit
CTGTGTGGGGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAG810.10143384885104251No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGGGAAAAAAAAAAAAAAAAAAA810.10143384885104251No Hit
CTGTGTGGGAGTGCAGTGCTCTGTGTGGGGACAATCCGCAGCGGCTTCTGAG810.10143384885104251No Hit
CTGTGTGGGACAAAATTTACATTTCCCTGGTGATAAAGGATGTTGAACAGTT800.10018157911214076No Hit
CTGTGTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTTCTGGCAGCATTTGA800.10018157911214076No Hit
CTGTGTGGGGATGCAGCCCCTCCTCTACAATTACAGGGAGAAAGAGGTCACG800.10018157911214076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCCC551.8189894E-1246.00000426
TTGTTCC1400.046.00000414
TCCTTGA453.0195224E-1046.00000432
CAATGGT351.0036638E-746.00000424
AAACTGC551.8189894E-1246.00000414
GGGTCCT1050.046.0000047
CGGAGGC351.0036638E-746.00000418
CCTATGG1500.046.00000422
CACATAT551.8189894E-1246.00000441
AGCCACG351.0036638E-746.00000413
CCTGTTG1050.046.00000411
ACTGAAG453.0195224E-1046.00000413
TTCAAGG453.0195224E-1046.00000434
CCTGCGC453.0195224E-1046.00000443
TCTGGTC551.8189894E-1246.00000424
GGTCCTG1050.046.0000048
GGTCTTA351.0036638E-746.00000416
CCAAAAT1100.046.00000444
GGGTAAG351.0036638E-746.00000429
TCGGTCT453.0195224E-1046.00000414
Produced by FastQC (version 0.11.5)