FastQCFastQC Report
Fri 17 Jun 2016
SRR1527222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20987
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGTTGGCATATCGACGGCGATCCGCTGGATTGCGCGAGCCAG8133.8738266545957023No Hit
GGGCGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6513.10192023633678No Hit
GGGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5752.739791299375804No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGA5672.701672463906228No Hit
GGGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC4572.177538476199552No Hit
GGGCGAGGGGTGACGGATTCTAATCGCGCTCCCGGCATGTTGTTGAATGC2931.3961023490732358No Hit
GGGCGAGGGACCCTGGTGTCGCTGTACGCCCGGTATTACATGTCGCCGGA2651.2626864249297183No Hit
GGGCGAGGGGGACGGCCGCGAACGTCTGAACGATCGTGCCGTGCCGACTG2571.224567589460142No Hit
GGGCGAGGGAGTGCAGTGCTGGGCGAGGGCACATCATGCGACCTGATGAA2321.1054462286177158No Hit
GGGCGAGGGAGCTCTGTGCACATCTGCCCCCTCCCCCCCTCACAGCCAGC2291.0911516653166247No Hit
GGGCGAGAGGCCGACGGTGATGCCGTTCCAGTTAATCAGCGTCGGAGTGG2251.0720922475818362No Hit
GGGCGAGGGGCACGATGACGAGATCGCCCTCGCGGCGGTGGCTTCGGTGC2201.048267975413351No Hit
GGGCGAGGGGCTTCACACTGACGCCGGAAGGGGTGAACCGCTTCCCGGTG1910.9100871968361367No Hit
GGGCGAGGGCAGCAGGCCGCCCCGGTGAATCTCAGCGACATAGGCGGTTT1700.8100252537284987No Hit
GGGCGAGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.7814361271263164No Hit
GGGCGAGGGCTCGGGAGGCTGAGGCAGGAGAATCTCTGGAACCCGGGAGG1610.7671415638252251No Hit
GGGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.7671415638252251No Hit
GGGCGAGGGAAGCAGTGGGGCGAGGGGGAATTCCTTCGATTCTGTTTTGC1470.7004336017534665No Hit
GGGCGAGGGGGCGGGGGGCTACTCAGGAGGCTGAGGCAGGAGAATGGCAT1450.6909038928860723No Hit
GGGCGAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.6813741840186782No Hit
GGGCGAGGGGCTGGGCGAGGGTGAGGCAGATGAAAATATTAAAGATTATG1410.6718444751512841No Hit
GGGCGAGGGTAGCCACGTGATCAGTGCATGATCAGTCACCTGATGGGTGT1400.6670796207175871No Hit
GGGCGAGGGAGTGCAGTGCTGGGCGAGGGGGAAAAAAAAAAAAAAAAAAA1400.6670796207175871No Hit
GGGCGAGGGAGAGCGCGCAGGATCTGCACTCCACCGTCCTTGGTCACAAG1350.6432553485491018No Hit
GGGCGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.6384904941154047No Hit
GGGCGAGGGGCTCAGGCTGACGTGCGGCCGACTCGAGGTACTTGCTGCCC1310.6241959308143136No Hit
GGGCGAGGGCACAATTATGTATTAGGACTTCAAGGATGAAAAGATCATCA1290.6146662219469196No Hit
GGGCGAGGGCAGTGCTGGGCGAGGGCTTTTTTTTTTTTTTTTTTTTTTTT1260.6003716586458284No Hit
GGGCGAGGGGGGCGAGGGGCTGGGCGAGGGTCAGAAAGAAATTCAACTGA1190.567017677609949No Hit
GGGCGAGGGGCACATAGTAGACATTCAATAACTGTGAGTTCTCTTTTGGT1180.5622528231762519No Hit
GGGCGAGGGGAGAATCTGCATTTTAACTGGTTACCCAGATGATTATTTCT1180.5622528231762519No Hit
GGGCGAGGGGAATCTTAGAATTGGAAGAGAAGTTGAAGATCAGTTAAGTC1140.5431934054414638No Hit
GGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG1120.5336636965740696No Hit
GGGCGAGCGCCAGAACGTTTTTTACCTTTAGACATTACATCACTCCTTCT1080.5146042788392815No Hit
GGGCGAAGGGAACTGAACTGAGTGGCTTTCAAGGTAGGATCTTACTTCCT1040.4955448611044933No Hit
GGGCGAGGGGAGGCAGGAGAATCACGTGAACCCGGGAGGCGGAGCTTGCA1040.4955448611044933No Hit
GGGCGAGGGAAGCAGTGGGAGTGCAGTGCTGGGCGAGGGCTGGGCGAGGG1020.4860151522370991No Hit
GGGCGAGGGCAGGTTCCTCCATCCACGGGATCTTCCAGGCAAGGGTACTG970.4621908800686139No Hit
GGGCGAGGGTTGTTCTCAGATTACATGATCTTGTACTTAGAAAAACCTAA870.4145423357316434No Hit
GGGCGAGGGGGCGAGGGGATACCACCAAAAAAAAAAAAAAAAAAAAAAAA860.40977748129794633No Hit
GGGCGAGGGCGGGCAGGTAGCCCATCTGCGCCGCCAGTACCGGGCCGACC840.40024777243055226No Hit
GGGCGAGGGATTGAGAAGACAGTGTGTGCCCGGCACCGGGCGAAAGGAGG840.40024777243055226No Hit
GGGCGAGGGGTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.3907180635631582No Hit
GGGCGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.3859532091294611No Hit
GGGCGAGGGGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTT800.38118835469576406No Hit
GGGCGAGGGAGCGATGAGTTGATTGTGCTGTCTCAAGTCGTTTTGTCAAT790.376423500262067No Hit
GGGCGAGGGCATCACAAAAGCATCGGTCTCCTTTTAAATGAATGAATGAA770.3668937913946729No Hit
GGGCGAGGGTTGAAGCAAAGAAGAGGCATCTGTTTCATATCTGCACACAC740.35259922809358174No Hit
GGGCGAGGGGTGCAGTGCTGGGCGAGGGCAGTGGTATCAACGCAAAAAAA730.34783437365988473No Hit
GGGCGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.34783437365988473No Hit
GGGCGAGGGGGCGTGGTGGCCCATGCCTGTAATCCTAGATACTCTGGAGG700.33353981035879354No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.33353981035879354No Hit
GGGCGAGGGGAAGTGACTGAGACAGGAAGTGAGCAAGCAAACGCAAGAGG670.31924524705770235No Hit
GGGCGAGGGGGGAATTCCTTCGATCCTGTTTTGCTACCCGTTGTAGCGCC660.31448039262400534No Hit
GGGCGAGGGGTGTCGAGGACGCGGCAACCTGACCCGCATCCTCGACACCC630.3001858293229142No Hit
GGGCGAGGGCCGTATGGTTGGCTGGCTATGAATTCTCGAAGCAGCATGAG620.29542097488921715No Hit
GGGCGAGGGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA610.2906561204555201No Hit
GGGCGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.2906561204555201No Hit
GGGCGAGGGCAGGCGGATTTCCCGGTACTCCCAGGAAATCCGCCTGGCCT570.2715967027207319No Hit
GGGCGAGGGGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAG550.26206699385333776No Hit
GGGCGAGGGTGATTAAACGCACCTTTAATCCGGGCTTCAAAATCGCTCTG550.26206699385333776No Hit
GGGCGAGGGTTTTCACTGACCCACTGCACTTCAATGAAAAAAGGCTGGTC490.23347786725115546No Hit
GGGCGAGGGCTGGGCGAGGGATGTAAAAAAATCAAGTTTCTCAGCTTATA480.22871301281745843No Hit
GGGCGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.21918330395006436No Hit
GGGCGAGGGATTACGCATAAGAACAAAAGATTTTGATAATCTTGTTTATG420.20012388621527613No Hit
GGGCGAAGGGAACCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATTGCATC420.20012388621527613No Hit
GGGCGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.20012388621527613No Hit
GGGCGAGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTT410.1953590317815791No Hit
GGGCGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA390.18582932291418497Illumina Single End Adapter 2 (100% over 34bp)
GGGCGAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA380.1810644684804879No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG380.1810644684804879Illumina Single End Adapter 2 (100% over 23bp)
GGGCGAGGGGGGCAGAGTCTGGCCTTGGTGGACCTTGAGCGGCGGGGCCC370.17629961404679087No Hit
GGGCGAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.1715347596130938No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG360.1715347596130938No Hit
GGGCGAGGGGTAAAACTTGTCAACGGAAAACCTAATCCAGACCCTCGCTC350.16676990517939677No Hit
GGGCGAGGGAGTGCTGGGCGAGGGAGCTTCAAAAAAAAAAAAAAAAAAAA340.16200505074569974No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC310.14771048744460857Illumina Single End Adapter 2 (95% over 22bp)
GGGCGAGGGGAAAAAGAAAAAAAAAAAGGGAAAAAAAAAAAAAAAAAAAA310.14771048744460857No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGG300.1429456330109115No Hit
GGGCGAGGGGATTCAAGAATCCAAATGATGCCAAAGGGAGGACAGTAATC290.13818077857721447No Hit
GGGCGAGGAGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.13818077857721447No Hit
GGGCGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1334159241435174No Hit
GGGCGAGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1334159241435174No Hit
GGGCGAGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.1334159241435174No Hit
GGGCGAGGGGTCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC270.12865106970982038No Hit
GGGCGAGGGGGTTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT270.12865106970982038No Hit
GGGCGAGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT260.12388621527612333No Hit
GGGCGAGGGCAGAGTGCAGTGCTGGGCGAGGGCAGTGCTGGGCGAGGGCA250.11912136084242626No Hit
GGGCGAGGGGCTGGGCGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11912136084242626No Hit
GGGCGAGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTT240.11435650640872921Illumina Single End Adapter 2 (96% over 33bp)
GGGCGAGGGGGTATCAACGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTTT230.10959165197503218No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGC230.10959165197503218No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCG230.10959165197503218No Hit
GGGCGAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTT220.10482679754133511No Hit
GGGCGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT220.10482679754133511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTT407.661583E-944.00000438
CTCGACA207.6806213E-444.00000441
CGACACC207.6806213E-444.00000443
ATCCTCG207.6806213E-444.00000438
CTTCACA207.6806213E-444.00000411
TTGTTGA407.661583E-944.00000440
TGTTGAA407.661583E-944.00000441
TCCTCGA207.6806213E-444.00000439
TCCTCCA207.6806213E-444.00000415
GGTGACG407.661583E-944.0000049
GTTGAAT407.661583E-944.00000442
TTCACAC207.6806213E-444.00000412
GGTGAAT207.6806213E-444.00000424
GGTGAAC207.6806213E-444.00000432
GTGTCGA207.6806213E-444.00000410
ACGCCGG207.6806213E-444.00000421
AATCTCA207.6806213E-444.00000428
GGGTCAC207.6806213E-444.0000048
ACGCCCG207.6806213E-444.00000426
AACCGCT207.6806213E-444.00000436
Produced by FastQC (version 0.11.5)