FastQCFastQC Report
Fri 17 Jun 2016
SRR1527221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22893
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGTTGGCATATCGACGGCGATCCGCTGGATTGCGCGAGCCAGGC8493.7085572008911023No Hit
GGGCGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6332.765037347660857No Hit
GGGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6102.6645699558817104No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAG5292.3107500109203687No Hit
GGGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG4742.0705019001441487No Hit
GGGCGAGGGGTGACGGATTCTAATCGCGCTCCCGGCATGTTGTTGAATGCAC2851.2449220285676843No Hit
GGGCGAGGGGGACGGCCGCGAACGTCTGAACGATCGTGCCGTGCCGACTGCC2601.135718341851221No Hit
GGGCGAGGGACCCTGGTGTCGCTGTACGCCCGGTATTACATGTCGCCGGAAG2551.1138776045079282No Hit
GGGCGAGAGGCCGACGGTGATGCCGTTCCAGTTAATCAGCGTCGGAGTGGCG2090.9129428209496353No Hit
GGGCGAGGGAGCTCTGTGCACATCTGCCCCCTCCCCCCCTCACAGCCAGCAG2020.8823657886690255No Hit
GGGCGAGGGGCACGATGACGAGATCGCCCTCGCGGCGGTGGCTTCGGTGCAG1980.8648931987943913No Hit
GGGCGAGGGAGTGCAGTGCTGGGCGAGGGCACATCATGCGACCTGATGAAGA1950.8517887563884157No Hit
GGGCGAGGGGCTTCACACTGACGCCGGAAGGGGTGAACCGCTTCCCGGTGCC1890.8255798715764644No Hit
GGGCGAGGGCAGCAGGCCGCCCCGGTGAATCTCAGCGACATAGGCGGTTTCG1820.7950028392958546No Hit
GGGCGAGGGCTCGGGAGGCTGAGGCAGGAGAATCTCTGGAACCCGGGAGGCA1540.6726947101734154No Hit
GGGCGAGGGGCTCAGGCTGACGTGCGGCCGACTCGAGGTACTTGCTGCCCAT1490.6508539728301227No Hit
GGGCGAGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.6333813829554885No Hit
GGGCGAGGGGCACATAGTAGACATTCAATAACTGTGAGTTCTCTTTTGGTCC1440.62901323548683No Hit
GGGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.6028043506748788No Hit
GGGCGAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.5984362032062203No Hit
GGGCGAGGGGGCGGGGGGCTACTCAGGAGGCTGAGGCAGGAGAATGGCATGA1360.5940680557375617No Hit
GGGCGAGGGAGTGCAGTGCTGGGCGAGGGGGAAAAAAAAAAAAAAAAAAAAA1310.572227318394269No Hit
GGGCGAGGGAAGCAGTGGGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTA1300.5678591709256104No Hit
GGGCGAGGGCACAATTATGTATTAGGACTTCAAGGATGAAAAGATCATCAGC1270.5547547285196348No Hit
GGGCGAGGGGCTGGGCGAGGGTGAGGCAGATGAAAATATTAAAGATTATGTG1230.5372821386450006No Hit
GGGCGAGGGCAGTGCTGGGCGAGGGCTTTTTTTTTTTTTTTTTTTTTTTTTT1200.524177696239025No Hit
GGGCGAGGGAGAGCGCGCAGGATCTGCACTCCACCGTCCTTGGTCACAAGGT1160.5067051063643908No Hit
GGGCGAAGGGAACTGAACTGAGTGGCTTTCAAGGTAGGATCTTACTTCCTCT1140.49796881142707383No Hit
GGGCGAGGGATTGAGAAGACAGTGTGTGCCCGGCACCGGGCGAAAGGAGGCA1130.4936006639584153No Hit
GGGCGAGGGGAGGCAGGAGAATCACGTGAACCCGGGAGGCGGAGCTTGCAGT1080.47175992661512256No Hit
GGGCGAGGGGAGAATCTGCATTTTAACTGGTTACCCAGATGATTATTTCTAT1030.44991918927182983No Hit
GGGCGAGGGGGGCGAGGGGCTGGGCGAGGGTCAGAAAGAAATTCAACTGATT1010.44118289433451274No Hit
GGGCGAGGGGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC980.4280784519285371No Hit
GGGCGAGGGCGGGCAGGTAGCCCATCTGCGCCGCCAGTACCGGGCCGACCAA950.41497400952256147No Hit
GGGCGAGGGGAATCTTAGAATTGGAAGAGAAGTTGAAGATCAGTTAAGTCAC940.4106058620539029No Hit
GGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA940.4106058620539029No Hit
GGGCGAGGGAAGCAGTGGGAGTGCAGTGCTGGGCGAGGGCTGGGCGAGGGCA890.38876512471061025No Hit
GGGCGAGGGTAGCCACGTGATCAGTGCATGATCAGTCACCTGATGGGTGTAT890.38876512471061025No Hit
GGGCGAGGGCAGGTTCCTCCATCCACGGGATCTTCCAGGCAAGGGTACTGGA850.3712925348359761No Hit
GGGCGAGGGGAAGTGACTGAGACAGGAAGTGAGCAAGCAAACGCAAGAGGCG840.36692438736731753No Hit
GGGCGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.35818809243000044No Hit
GGGCGAGGGCATCACAAAAGCATCGGTCTCCTTTTAAATGAATGAATGAATG810.3538199449613419No Hit
GGGCGAGGGGTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.34945179749268335No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.3450836500240248No Hit
GGGCGAGGGGGCGAGGGGATACCACCAAAAAAAAAAAAAAAAAAAAAAAAAA770.3363473550867077No Hit
GGGCGAGGGTTGTTCTCAGATTACATGATCTTGTACTTAGAAAAACCTAAAG740.3232429126807321No Hit
GGGCGAGGGGGCGTGGTGGCCCATGCCTGTAATCCTAGATACTCTGGAGGCT710.31013847027475644No Hit
GGGCGAGGGAGCGATGAGTTGATTGTGCTGTCTCAAGTCGTTTTGTCAATCC700.30577032280609795No Hit
GGGCGAGGGTTGAAGCAAAGAAGAGGCATCTGTTTCATATCTGCACACACAT690.3014021753374394No Hit
GGGCGAGGGCAGGCGGATTTCCCGGTACTCCCAGGAAATCCGCCTGGCCTCG680.29703402786878086No Hit
GGGCGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.29703402786878086No Hit
GGGCGAGGGGTGTCGAGGACGCGGCAACCTGACCCGCATCCTCGACACCCGC670.2926658804001223No Hit
GGGCGAGGGGTGCAGTGCTGGGCGAGGGCAGTGGTATCAACGCAAAAAAAAA630.27519329052548813No Hit
GGGCGAGGGGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGC630.27519329052548813No Hit
GGGCGAGGGGGGAATTCCTTCGATCCTGTTTTGCTACCCGTTGTAGCGCCGG610.26645699558817104No Hit
GGGCGAGGGGGGCAGAGTCTGGCCTTGGTGGACCTTGAGCGGCGGGCCCTCG560.24461625824487837No Hit
GGGCGAGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAG560.24461625824487837No Hit
GGGCGAGGGTTTTCACTGACCCACTGCACTTCAATGAAAAAAGGCTGGTCAA550.24024811077621983No Hit
GGGCGAGGGTGATTAAACGCACCTTTAATCCGGGCTTCAAAATCGCTCTGCA540.23587996330756128No Hit
GGGCGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.23587996330756128No Hit
GGGCGAGGGATTACGCATAAGAACAAAAGATTTTGATAATCTTGTTTATGAC490.21403922596426855No Hit
GGGCGAGGGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAAAA480.20967107849561No Hit
GGGCGAGGGGGGAATACCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG470.20530293102695146No Hit
GGGCGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.20530293102695146No Hit
GGGCGAGCGCCAGAACGTTTTTTACCTTTAGACATTACATCACTCCTTCTGC460.20093478355829292No Hit
GGGCGAGGGCTGGGCGAGGGATGTAAAAAAATCAAGTTTCTCAGCTTATACG450.19656663608963437No Hit
GGGCGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.1878303411523173No Hit
GGGCGAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.18346219368365876No Hit
GGGCGAGGGCCGTATGGTTGGCTGGCTATGAATTCTCGAAGCAGCATGAGGC420.18346219368365876No Hit
GGGCGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.16598960380902458No Hit
GGGCGAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGAT360.1572533088717075No Hit
GGGCGAGGGGAGTGGCTGGGCGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA360.1572533088717075No Hit
GGGCGAGGGGGACGGACGCGAACGTCTGAACGATCGTGCCGTGCCGACTGCC350.15288516140304897No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC330.14414886646573188Illumina Single End Adapter 2 (100% over 25bp)
GGGCGAGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.13978071899707334No Hit
GGGCGAGGGAGTGCTGGGCGAGGGAGCTTCAAAAAAAAAAAAAAAAAAAAAA310.1354125715284148No Hit
GGGCGAAGGGAACCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATTGCATCAC310.1354125715284148No Hit
GGGCGAGGGGTTGGCGTATCGACGGCGATCCGCTGGATTGCGCGAGCCAGGC310.1354125715284148No Hit
GGGCGAGGGCGAATCGGATTTCATCATCCACCTTGGGCCACGCCCTTGATCA300.13104442405975625No Hit
GGGCGAGGGGTGACGAATTCTAATCGCGCTCCCGGCATGTTGTTGAATGCAC300.13104442405975625No Hit
GGGCGAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT300.13104442405975625No Hit
GGGCGAGGGAACGCAGAGTGCAGTGCTGGGCGAGGGTTGTTCTGGCTGCGGG290.1266762765910977No Hit
GGGCGAGGGAAGCAGAGGGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTA290.1266762765910977No Hit
GGGCGAGGAGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.12230812912243919No Hit
GGGCGAGGGGATTCAAGAATCCAAATGATGCCAAAGGGAGGACAGTAATCCA270.11793998165378064No Hit
GGGCGAGGGAGCGGCGTAGCTGTAAGTCTTGGCCATTTCGTTCACCTGTTTG270.11793998165378064No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT270.11793998165378064Illumina Single End Adapter 2 (95% over 24bp)
GGGCGAGGGGATGGATTCAGTAAGAACAGCGCCTGGAACTAGAAGTGGGGGA270.11793998165378064No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGGAG270.11793998165378064No Hit
GGGCGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA270.11793998165378064No Hit
GGGCGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA260.1135718341851221No Hit
GGGCGAGAGGCCGACAGTGATGCCGTTCCAGTTAATCAGCGTCGGAGTGGCG260.1135718341851221No Hit
GGGCGAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.1135718341851221No Hit
GGGCGAGGGGGTATCAACGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT260.1135718341851221No Hit
GGGCGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA250.10920368671646355Illumina Single End Adapter 2 (100% over 34bp)
GGGCGAGGGAGTGCAATGCTGGGCGAGGGCACATCATGCGACCTGATGAAGA250.10920368671646355No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA250.10920368671646355No Hit
GGGCGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG250.10920368671646355No Hit
GGGCGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.104835539247805No Hit
GGGCGAGGGGCACGAAGACGAGATCGCCCTCGCGGCGGTGGCTTCGGTGCAG240.104835539247805No Hit
GGGCGAGGGGTAAAACTTGTCAACGGAAAACCTAATCCAGACCCTCGCTCTA240.104835539247805No Hit
GGGCGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.10046739177914646No Hit
GGGCGAGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA230.10046739177914646Illumina Single End Adapter 2 (97% over 34bp)
GGGCGAGGGGGTTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT230.10046739177914646No Hit
GGGCGAGGGGGAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.10046739177914646No Hit
GGGCGAGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA230.10046739177914646Illumina Single End Adapter 2 (100% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGATG359.620271E-846.00000416
GGGTTGG700.046.0000048
CCGTGCC550.046.00000439
CAGCAGG359.620271E-846.00000410
TCGACGG700.046.00000419
TGCCGTG550.046.00000437
CGCCCCG359.620271E-846.00000418
GCGATCC700.046.00000425
TGCCGAC550.046.00000442
GGCCGCC359.620271E-846.00000415
GCAGGCC359.620271E-846.00000412
GCGGTTT359.620271E-846.00000444
GACGGTG359.620271E-846.00000413
CCGCCCC359.620271E-846.00000417
ATAGGCG359.620271E-846.00000440
TATCGAC700.046.00000417
GACGGCG700.046.00000421
GACGGAT550.046.00000412
GGATTGC700.046.00000435
CGGATTC550.046.00000414
Produced by FastQC (version 0.11.5)