FastQCFastQC Report
Fri 17 Jun 2016
SRR1527220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22499
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGTTGGCATATCGACGGCGATCCGCTGGATTGCGCGAGCCAGGC8253.6668296368727495No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGAAG5602.4889995110893817No Hit
GGGCGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5402.400106671407618No Hit
GGGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5232.324547757678119No Hit
GGGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG4562.026756744744211No Hit
GGGCGAGGGACCCTGGTGTCGCTGTACGCCCGGTATTACATGTCGCCGGAAG2801.2444997555446908No Hit
GGGCGAGGGGGACGGCCGCGAACGTCTGAACGATCGTGCCGTGCCGACTGCC2651.177830125783368No Hit
GGGCGAGGGGTGACGGATTCTAATCGCGCTCCCGGCATGTTGTTGAATGCAC2561.1378283479265745No Hit
GGGCGAGGGAGCTCTGTGCACATCTGCCCCCTCCCCCCCTCACAGCCAGCAG2020.8978176807858127No Hit
GGGCGAGGGGCACGATGACGAGATCGCCCTCGCGGCGGTGGCTTCGGTGCAG1990.8844837548335482No Hit
GGGCGAGAGGCCGACGGTGATGCCGTTCCAGTTAATCAGCGTCGGAGTGGCG1880.8355926930085782No Hit
GGGCGAGGGAGTGCAGTGCTGGGCGAGGGCACATCATGCGACCTGATGAAGA1870.83114805102449No Hit
GGGCGAGGGGCTTCACACTGACGCCGGAAGGGGTGAACCGCTTCCCGGTGCC1690.7511444953109028No Hit
GGGCGAGGGGCTCAGGCTGACGTGCGGCCGACTCGAGGTACTTGCTGCCCAT1650.73336592737455No Hit
GGGCGAGGGAGTGCAGTGCTGGGCGAGGGGGAAAAAAAAAAAAAAAAAAAAA1630.7244766434063736No Hit
GGGCGAGGGCTCGGGAGGCTGAGGCAGGAGAATCTCTGGAACCCGGGAGGCA1580.7022534334859327No Hit
GGGCGAGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.6044713098359927No Hit
GGGCGAGGGCAGCAGGCCGCCCCGGTGAATCTCAGCGACATAGGCGGTTTCG1360.6044713098359927No Hit
GGGCGAGGGGCTGGGCGAGGGTGAGGCAGATGAAAATATTAAAGATTATGTG1350.6000266678519045No Hit
GGGCGAGGGCACAATTATGTATTAGGACTTCAAGGATGAAAAGATCATCAGC1320.58669274189964No Hit
GGGCGAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.5600248899951109No Hit
GGGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.5555802480110227No Hit
GGGCGAGGGGCACATAGTAGACATTCAATAACTGTGAGTTCTCTTTTGGTCC1240.5511356060269346No Hit
GGGCGAGGGGGCGGGGGGCTACTCAGGAGGCTGAGGCAGGAGAATGGCATGA1230.5466909640428463No Hit
GGGCGAGGGTAGCCACGTGATCAGTGCATGATCAGTCACCTGATGGGTGTAT1230.5466909640428463No Hit
GGGCGAGGGAGAGCGCGCAGGATCTGCACTCCACCGTCCTTGGTCACAAGGT1170.5200231121383172No Hit
GGGCGAGGGAAGCAGTGGGAGTGCAGTGCTGGGCGAGGGCTGGGCGAGGGCA1110.4933552602337882No Hit
GGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1070.4755766922974355No Hit
GGGCGAGGGCAGTGCTGGGCGAGGGCTTTTTTTTTTTTTTTTTTTTTTTTTT1070.4755766922974355No Hit
GGGCGAGGGAAGCAGTGGGGCGAGGGGGAATTCCTTCGATTCTGTTTTGCTA1050.46668740832925903No Hit
GGGCGAAGGGAACTGAACTGAGTGGCTTTCAAGGTAGGATCTTACTTCCTCT1010.44890884039290635No Hit
GGGCGAGGGGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC980.43557491444064184No Hit
GGGCGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.4177963465042891No Hit
GGGCGAGGGGAGGCAGGAGAATCACGTGAACCCGGGAGGCGGAGCTTGCAGT940.4177963465042891No Hit
GGGCGAGGGGGGCGAGGGGCTGGGCGAGGGTCAGAAAGAAATTCAACTGATT900.40001777856793636No Hit
GGGCGAGGGTTGTTCTCAGATTACATGATCTTGTACTTAGAAAAACCTAAAG880.39112849459975996No Hit
GGGCGAGGGCATCACAAAAGCATCGGTCTCCTTTTAAATGAATGAATGAATG860.3822392106315836No Hit
GGGCGAGGGATTGAGAAGACAGTGTGTGCCCGGCACCGGGCGAAAGGAGGCA860.3822392106315836No Hit
GGGCGAGGGGAATCTTAGAATTGGAAGAGAAGTTGAAGATCAGTTAAGTCAC850.37779456864749544No Hit
GGGCGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.37334992666340727No Hit
GGGCGAGGGGAGAATCTGCATTTTAACTGGTTACCCAGATGATTATTTCTAT830.36890528467931905No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.3644606426952309No Hit
GGGCGAGGGCAGGTTCCTCCATCCACGGGATCTTCCAGGCAAGGGTACTGGA820.3644606426952309No Hit
GGGCGAGGGAGCGATGAGTTGATTGTGCTGTCTCAAGTCGTTTTGTCAATCC820.3644606426952309No Hit
GGGCGAGGGGAAGTGACTGAGACAGGAAGTGAGCAAGCAAACGCAAGAGGCG810.3600160007111427No Hit
GGGCGAGGGGTGTCGAGGACGCGGCAACCTGACCCGCATCCTCGACACCCGC730.3244588648384373No Hit
GGGCGAGGGGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGC730.3244588648384373No Hit
GGGCGAGGGGTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.3111249388861727No Hit
GGGCGAGGGGGCGAGGGGATACCACCAAAAAAAAAAAAAAAAAAAAAAAAAA690.30668029690208454No Hit
GGGCGAGGGCCGTATGGTTGGCTGGCTATGAATTCTCGAAGCAGCATGAGGC690.30668029690208454No Hit
GGGCGAGGGCGGGCAGGTAGCCCATCTGCGCCGCCAGTACCGGGCCGACCAA660.29334637094982No Hit
GGGCGAGGGTTGAAGCAAAGAAGAGGCATCTGTTTCATATCTGCACACACAT650.2889017289657318No Hit
GGGCGAGGGGTGCAGTGCTGGGCGAGGGCAGTGGTATCAACGCAAAAAAAAA630.28001244499755545No Hit
GGGCGAGGGCAGGCGGATTTCCCGGTACTCCCAGGAAATCCGCCTGGCCTCG590.2622338770612027No Hit
GGGCGAGGGGGCGTGGTGGCCCATGCCTGTAATCCTAGATACTCTGGAGGCT540.24001066714076183No Hit
GGGCGAGGGGGGCAGAGTCTGGCCTTGGTGGACCTTGAGCGGCGGGCCCTCG520.23112138317258543No Hit
GGGCGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.23112138317258543No Hit
GGGCGAGGGTTTTCACTGACCCACTGCACTTCAATGAAAAAAGGCTGGTCAA500.2222320992044091No Hit
GGGCGAGCGCCAGAACGTTTTTTACCTTTAGACATTACATCACTCCTTCTGC480.21334281523623272No Hit
GGGCGAGGGGGGAATTCCTTCGATCCTGTTTTGCTACCCGTTGTAGCGCCGG480.21334281523623272No Hit
GGGCGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.21334281523623272No Hit
GGGCGAGGGTGATTAAACGCACCTTTAATCCGGGCTTCAAAATCGCTCTGCA470.20889817325214455No Hit
GGGCGAGGGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAAAA470.20889817325214455No Hit
GGGCGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.20889817325214455No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC450.20000888928396818Illumina Single End Adapter 2 (100% over 25bp)
GGGCGAGGGCTGGGCGAGGGATGTAAAAAAATCAAGTTTCTCAGCTTATACG450.20000888928396818No Hit
GGGCGAGGGGTTGGCATATCGACGGCGATCCGCTGGATTACGCGAGCCAGGC430.1911196053157918No Hit
GGGCGAGGGATTACGCATAAGAACAAAAGATTTTGATAATCTTGTTTATGAC420.18667496333170364No Hit
GGGCGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.17778567936352727No Hit
GGGCGAAGGGAACCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATTGCATCAC380.1688963953953509No Hit
GGGCGAGGGAACGCAGAGTGCAGTGCTGGGCGAGGGTTGTTCTGGCTGCGGG360.16000711142717455No Hit
GGGCGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG350.15556246944308635No Hit
GGGCGAGGGAAGCAGTGGTAGCAACGCAGAGTGCAGTGCAGGGCGAGGGAAG320.1422285434908218No Hit
GGGCGAGGGGAAAAAGAAAAAAAAAAAGGGAAAAAAAAAAAAAAAAAAAAAA320.1422285434908218No Hit
GGGCGAGGGAACGCAGAGTGCAGTGCTGGGCGAGGGTTGTTCTGGCTGCGGT320.1422285434908218No Hit
GGGCGAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.13778390150673364No Hit
GGGCGAGGGGGAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.13778390150673364No Hit
GGGCGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA300.13333925952264547Illumina Single End Adapter 2 (100% over 34bp)
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGCGAGGGGAG300.13333925952264547No Hit
GGGCGAGGAGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.13333925952264547No Hit
GGGCGAGGGAGTGCTGGGCGAGGGAGCTTCAAAAAAAAAAAAAAAAAAAAAA290.12889461753855727No Hit
GGGCGAGGGGAGTGGCTGGGCGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA290.12889461753855727No Hit
GGGCGAGGGGATGGATTCAGTAAGAACAGCGCCTGGAACTAGAAGTGGGGGA280.12444997555446909No Hit
GGGCGAGGGGAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.12444997555446909No Hit
GGGCGAGGGGATTCAAGAATCCAAATGATGCCAAAGGGAGGACAGTAATCCA270.12000533357038091No Hit
GGGCGAGGGGGTTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT270.12000533357038091No Hit
GGGCGAGGGCGAATCGGATTTCATCATCCACCTTGGGCCACGCCCTTGATCA260.11556069158629272No Hit
GGGCGAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGAT260.11556069158629272No Hit
GGGCGAGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.11556069158629272No Hit
GGGCGAGGGGTAAAACTTGTCAACGGAAAACCTAATCCAGACCCTCGCTCTA260.11556069158629272No Hit
GGGCGAGGGGGTATCAACGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT260.11556069158629272No Hit
GGGCGAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT260.11556069158629272No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT250.11111604960220454Illumina Single End Adapter 2 (95% over 24bp)
GGGCGAGGGCAGAGTGCAGTGCTGGGCGAGGGCAGTGCTGGGCGAGGGCAGT250.11111604960220454No Hit
GGGCGAGGGAGCGCGTTGCAGCTCATCAATTTCTGGCAAGACCCGAGCGTCG250.11111604960220454No Hit
GGGCGAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11111604960220454No Hit
GGGCGAGGGAGGTGAGCAAACTGAGGCCTGGGAGCAGTTGGCTTTCCCAAGG240.10667140761811636No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA240.10667140761811636No Hit
GGGCGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA230.10222676563402817No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT230.10222676563402817Illumina Single End Adapter 2 (96% over 26bp)
GGGCGAGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.10222676563402817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCACA359.610085E-846.00000411
TTCACAC359.610085E-846.00000412
CCGTGCC359.610085E-846.00000439
TGCCGAC359.610085E-846.00000442
GACGGCC359.610085E-846.00000411
AAGGGGT359.610085E-846.00000428
AGGGAGA359.610085E-846.0000046
GGGGGAC359.610085E-846.0000047
GGGACGG359.610085E-846.0000049
GACTGCC359.610085E-846.00000446
GGGGCTT359.610085E-846.0000047
TCACACT359.610085E-846.00000413
GGGGACG359.610085E-846.0000048
GCTTCAC359.610085E-846.00000410
GGGTGAA359.610085E-846.00000431
CGACTGC359.610085E-846.00000445
GGACGGC359.610085E-846.00000410
GTGCCGA359.610085E-846.00000441
GAAGGGG359.610085E-846.00000427
TGTTGTT301.7806415E-646.038
Produced by FastQC (version 0.11.5)