SRR1527219_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595556
Sequences flagged as poor quality	0
Sequence length	50
%GC	59

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACACCCGGGACCACATTTCTACACTTTTAATACGTCAGTGTCCATGTTAA	3111	0.5223690131574529	No Hit
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2804	0.470820544163773	No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGA	2758	0.46309666933084376	No Hit
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2676	0.4493280228895352	No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	2473	0.41524222743117356	No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	2359	0.3961004506713055	No Hit
ACACCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	2020	0.33917885135906617	No Hit
ACACCCGGGGTCCTTTACTTTCCCTCCTGACAGATAGTCTAGAAGCCGAT	1997	0.33531691394260155	No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1956	0.3284325907219472	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1521	0.25539160045402953	No Hit
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1517	0.2547199591642096	No Hit
ACACCCGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGAC	1263	0.21207073726064382	No Hit
ACACCCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	1262	0.21190282693818885	No Hit
ACACCCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAAC	1208	0.20283566952561974	No Hit
ACACCCGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAG	1173	0.19695880823969533	No Hit
ACACCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1171	0.19662298759478536	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1144	0.19208940888850085	No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1123	0.18856329211694617	No Hit
ACACCCCGTTTCCCGGCTTCTCAATGCCTCGTGATGGTATATGTTTAACA	1112	0.18671627856994136	No Hit
ACACCCGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC	1092	0.1833580721208417	No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1060	0.17798494180228222	No Hit
ACACCCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1039	0.17445882503072757	No Hit
ACACCCGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTC	1032	0.1732834527735427	No Hit
ACACCCGGAGGCCCAGTGCAGTTCTCTGGAGAAAGCTACCTTTTCCCAAG	1026	0.17227599083881281	No Hit
ACACCCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1017	0.17076479793671798	No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	1000	0.16791032245498325	No Hit
ACACCCGGGATTGAAAAAGGGTCTTTGTTTAGCCCAGGCTGGCCTCATAC	941	0.15800361343013922	No Hit
ACACCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG	889	0.1492722766624801	No Hit
ACACCCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA	872	0.14641780118074538	No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGT	867	0.14557824956847049	No Hit
ACACCCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	858	0.14406705666637562	No Hit
ACACCCGGGCAAATGTAAGAAGTCCCTCACATCCGATTAGCCCAACTCTG	854	0.14339541537655567	No Hit
ACACCCGGGATGGGATTCATTGGCTTCTTCGTGAAACTGATCCATATACC	814	0.13667900247835635	No Hit
ACACCCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA	804	0.1349998992538065	No Hit
ACACCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	777	0.13046632054752197	No Hit
ACACCCGGGGAACTAAAGTAACTCATATTTATGTAGTAGAATGCTTATGT	772	0.12962676893524705	No Hit
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	740	0.1242536386166876	No Hit
ACACCCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC	725	0.12173498377986286	No Hit
ACACCCGGGATTGTCAATTTTTCTGACTTTTTAAATCATCATCATTATTT	723	0.12139916313495289	No Hit
ACACCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT	721	0.12106334249004291	No Hit
ACACCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	712	0.11955214958794806	No Hit
ACACCCGGGATGGGATTCAGGTTGAGCCCCCAAACTGTGAATTCAGTTGC	701	0.11770513604094325	No Hit
ACACCCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	689	0.11569021217148347	No Hit
ACACCCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	681	0.11434692959184359	No Hit
ACACCCGGGGGCCGAGCTCGTCGCTAGGTTTGTTGAAGGACATCGGCCGC	666	0.11182827475501883	No Hit
ACACCCGGCAGTGTCATTCCTATGAGGCCTGTGCCAAGTTGGTGATGGAT	651	0.10930961991819409	No Hit
ACACCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	650	0.1091417095957391	No Hit
ACACCCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGT	641	0.10763051669364426	No Hit
ACACCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCT	640	0.10746260637118928	No Hit
ACACCCGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	639	0.10729469604873429	No Hit
ACACCCGGGATTCCTGATGGCTGTGGTGTCAAATATATCCCCAATCGTGG	633	0.1062872341140044	No Hit
ACACCCGGGGACACCAAACTGGTTCTTGAACCTCTCGGCCCCGATTCTGA	613	0.10292902766490472	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.853421E-4	44.000004	41
TATTTCG	20	7.853421E-4	44.000004	42
AGCGTAC	45	4.783942E-10	44.000004	10
TCGTTTA	20	7.853421E-4	44.000004	18
GATACTC	20	7.853421E-4	44.000004	10
AGGTACA	20	7.853421E-4	44.000004	42
ACTATAT	20	7.853421E-4	44.000004	25
TCGTTAA	20	7.853421E-4	44.000004	16
ATCTCCG	20	7.853421E-4	44.000004	25
CGACGGG	20	7.853421E-4	44.000004	36
CATGCGC	45	4.783942E-10	44.000004	14
TAGACGG	20	7.853421E-4	44.000004	24
GCGAAAT	20	7.853421E-4	44.000004	28
CATGATA	20	7.853421E-4	44.000004	40
CATGACG	20	7.853421E-4	44.000004	34
CATTGCG	20	7.853421E-4	44.000004	24
TTAGACG	20	7.853421E-4	44.000004	23
CGGTTAT	20	7.853421E-4	44.000004	14
TTATGCC	20	7.853421E-4	44.000004	23
CCAATAC	20	7.853421E-4	44.000004	38

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content