##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595556
Sequences flagged as poor quality	0
Sequence length	50
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35791596424182	33.0	31.0	34.0	31.0	34.0
2	32.68062281296805	34.0	31.0	34.0	31.0	34.0
3	32.66956423913116	34.0	31.0	34.0	31.0	34.0
4	36.36456353390781	37.0	37.0	37.0	35.0	37.0
5	36.432313669915175	37.0	37.0	37.0	35.0	37.0
6	36.41437413106408	37.0	37.0	37.0	35.0	37.0
7	36.47283378893001	37.0	37.0	37.0	35.0	37.0
8	36.613737415121335	37.0	37.0	37.0	35.0	37.0
9	38.7083330534828	39.0	39.0	39.0	39.0	39.0
10	38.18886888890381	39.0	39.0	39.0	37.0	39.0
11	37.94492373513154	39.0	38.0	39.0	35.0	39.0
12	37.365487040681316	39.0	37.0	39.0	35.0	39.0
13	37.20758585254787	39.0	37.0	39.0	34.0	39.0
14	38.04549530186918	40.0	38.0	40.0	34.0	40.0
15	38.046420487745905	40.0	38.0	40.0	34.0	40.0
16	37.90193029706694	40.0	38.0	40.0	34.0	40.0
17	38.0180016656704	40.0	38.0	40.0	34.0	40.0
18	37.972415692227095	40.0	38.0	40.0	34.0	40.0
19	37.76640483850385	40.0	37.0	40.0	34.0	40.0
20	37.56961394058661	40.0	37.0	40.0	33.0	40.0
21	37.731738073329794	40.0	37.0	40.0	33.0	40.0
22	37.528225725204685	40.0	37.0	40.0	33.0	40.0
23	37.590374372854946	40.0	37.0	40.0	33.0	40.0
24	37.63282546057801	40.0	37.0	40.0	33.0	40.0
25	37.50305596786868	40.0	36.0	40.0	33.0	40.0
26	37.38250811006858	40.0	36.0	40.0	33.0	40.0
27	37.186115159615554	39.0	35.0	40.0	33.0	40.0
28	36.97537259300553	39.0	35.0	40.0	32.0	40.0
29	37.16104614847303	39.0	35.0	40.0	33.0	40.0
30	37.12745736756913	39.0	35.0	40.0	33.0	40.0
31	37.01387946725413	39.0	35.0	40.0	33.0	40.0
32	36.14716164390922	39.0	35.0	40.0	30.0	40.0
33	36.35481801879252	39.0	35.0	40.0	31.0	40.0
34	36.3119941701536	38.0	35.0	40.0	31.0	40.0
35	36.306389323590054	38.0	35.0	40.0	31.0	40.0
36	35.98283620683865	38.0	35.0	40.0	30.0	40.0
37	35.77416061629805	38.0	35.0	40.0	30.0	40.0
38	35.533654265929655	37.0	35.0	40.0	29.0	40.0
39	35.50361678834568	37.0	35.0	40.0	30.0	40.0
40	35.36874785914339	37.0	35.0	40.0	29.0	40.0
41	35.20689742022581	37.0	35.0	40.0	29.0	40.0
42	35.06249454291452	36.0	34.0	40.0	28.0	40.0
43	34.940433813109095	36.0	34.0	40.0	28.0	40.0
44	34.735962697042766	35.0	34.0	40.0	28.0	40.0
45	34.596684442772805	35.0	34.0	40.0	27.0	40.0
46	34.329572701811415	35.0	34.0	39.0	26.0	40.0
47	34.208420366850476	35.0	34.0	39.0	26.0	40.0
48	34.09292828885948	35.0	34.0	39.0	26.0	40.0
49	33.76783543445117	35.0	33.0	39.0	26.0	40.0
50	33.36790830753112	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	2.0
15	7.0
16	13.0
17	37.0
18	88.0
19	180.0
20	334.0
21	551.0
22	908.0
23	1356.0
24	1964.0
25	2803.0
26	4156.0
27	5552.0
28	7197.0
29	9004.0
30	11717.0
31	13977.0
32	16902.0
33	22760.0
34	41335.0
35	56245.0
36	75350.0
37	107725.0
38	147731.0
39	67658.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	98.52507572755542	0.5673689795753883	0.1894028437292211	0.7181524491399633
8	99.10772454647422	0.38317135584227174	0.17563419728791246	0.3334699003955967
9	97.13931183633446	1.5501480969044052	0.6986748517351853	0.6118652150259589
10	60.71099946940338	27.564998085822324	5.445163846892651	6.278838597881643
11	37.47103546937652	24.15759391224335	19.143959594060004	19.227411024320133
12	30.629361470625767	13.98944851533693	28.03229251321454	27.348897500822762
13	23.706250965484355	16.565528682441283	28.763206146861087	30.965014205213283
14	23.7415121331999	19.070246962502267	29.077198449851906	28.11104245444593
15	25.813021781327027	19.214481929491097	28.567590621201028	26.404905667980845
16	30.246693845750862	17.104017086554414	28.713336781091954	23.93595228660277
17	31.867700098731273	18.258400553432423	21.955282122923787	27.918617224912516
18	30.68225322219909	18.87899710522604	23.142576013002973	27.2961736595719
19	30.64195474480989	17.9123373788527	23.734291989334334	27.71141588700307
20	29.731712886781423	21.226383413146706	24.355224361772866	24.686679338299
21	30.09708574844347	17.583904788130756	26.823506101861117	25.49550336156465
22	29.416545211533425	15.912189617768943	25.668115173048378	29.00314999764926
23	27.59287119934985	22.271457260106523	23.817743419594464	26.317928120949162
24	28.259475179496135	21.089200679700983	23.50593395079556	27.145390190007323
25	27.992665677115163	20.778230762514358	24.221231924453786	27.007871635916693
26	30.539697358434807	19.532504080220836	21.969722410654917	27.958076150689436
27	27.015763421072077	17.32767363606445	23.883900086641727	31.77266285622175
28	27.682031580573447	20.26274607257756	23.5814935959003	28.473728750948695
29	31.00984626130876	19.95177615539093	21.65656965927637	27.38180792402394
30	26.472909348575115	21.295730376320616	23.133173034945496	29.098187240158772
31	31.612140587954784	19.942205267010994	22.08776336734077	26.35789077769345
32	30.587551800334477	21.03379027329084	22.74781884491131	25.630839081463375
33	34.19376179570015	18.72485542921237	21.296737838255346	25.784644936832134
34	30.2935408257158	22.270449798171793	22.63531892886647	24.800690447245934
35	30.708783053146977	23.28462814579989	24.884645608473427	21.121943192579707
36	32.266487114561855	22.114964839578477	21.74707332307961	23.871474722780057
37	30.250052052199962	22.905990368663904	21.32242811759096	25.521529461545178
38	34.1969520918268	21.650860708312905	21.868976217181928	22.28321098267837
39	30.174660317417672	20.65699950970186	20.879648597277168	28.288691575603302
40	27.77169569276441	22.587296576644345	23.87735158406598	25.763656146525264
41	26.803524773488974	22.71524424235504	22.332072886512773	28.149158097643213
42	27.106434995197766	19.90056350704216	23.919664985324637	29.07333651243544
43	25.682051729812144	20.984424638489077	25.619253269213978	27.714270362484804
44	25.947685859935927	20.05991040305194	25.635036839524744	28.357366897487392
45	26.618823418788494	19.17854912048573	25.103264848309813	29.099362612415963
46	28.874698600971193	18.70974350019142	23.750411380290014	28.665146518547374
47	27.98628508486188	21.051924588115977	24.071959647791306	26.889830679230837
48	30.728260650551753	20.284574414496706	24.511884692623365	24.475280242328175
49	29.027664904727686	22.47647576382406	23.67686665905473	24.818992672393527
50	25.89764858384434	22.214367750471826	25.35513033199229	26.53285333369154
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	3.5
10	4.0
11	20.0
12	36.0
13	131.5
14	227.0
15	316.5
16	406.0
17	502.0
18	598.0
19	674.5
20	751.0
21	715.0
22	679.0
23	663.0
24	647.0
25	807.0
26	967.0
27	1152.5
28	1338.0
29	2026.5
30	2715.0
31	3265.0
32	3815.0
33	4291.0
34	4767.0
35	5066.0
36	5365.0
37	5751.5
38	6138.0
39	6351.5
40	6565.0
41	9050.0
42	11535.0
43	11232.5
44	10930.0
45	11588.0
46	12246.0
47	15839.0
48	19432.0
49	18138.5
50	16845.0
51	19977.5
52	23110.0
53	23810.5
54	24511.0
55	27598.5
56	30686.0
57	34450.5
58	38215.0
59	47414.5
60	56614.0
61	58915.0
62	61216.0
63	60086.5
64	58957.0
65	55823.0
66	52689.0
67	48007.5
68	43326.0
69	36076.5
70	28827.0
71	26347.5
72	23868.0
73	21344.0
74	18820.0
75	15335.0
76	11850.0
77	11203.5
78	10557.0
79	7440.5
80	4324.0
81	2754.0
82	1184.0
83	797.5
84	411.0
85	367.5
86	324.0
87	178.5
88	33.0
89	25.5
90	18.0
91	12.5
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	595556.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.480270537111538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.4602634305165	8.793631497289928
2	11.941824639767514	2.9807440442208626
3	4.891896618994443	1.8315657973389572
4	2.5132186150389497	1.2546259293836348
5	1.380386669716254	0.861379954194064
6	0.9175669675891668	0.6870890394857914
7	0.49914566711962	0.4360631074155915
8	0.32693368493279695	0.3264176668524874
9	0.2825352832752566	0.3173505094399183
>10	2.7890268677600334	8.943407504919772
>50	1.933348581269256	17.484837697882316
>100	1.9642929218184508	43.81384790011351
>500	0.06592489943089322	5.399156418539986
>1k	0.03363515277086389	6.869882932923184
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACACCCGGGACCACATTTCTACACTTTTAATACGTCAGTGTCCATGTTAA	3111	0.5223690131574529	No Hit
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2804	0.470820544163773	No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGA	2758	0.46309666933084376	No Hit
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2676	0.4493280228895352	No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	2473	0.41524222743117356	No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	2359	0.3961004506713055	No Hit
ACACCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	2020	0.33917885135906617	No Hit
ACACCCGGGGTCCTTTACTTTCCCTCCTGACAGATAGTCTAGAAGCCGAT	1997	0.33531691394260155	No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1956	0.3284325907219472	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1521	0.25539160045402953	No Hit
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1517	0.2547199591642096	No Hit
ACACCCGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGAC	1263	0.21207073726064382	No Hit
ACACCCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	1262	0.21190282693818885	No Hit
ACACCCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAAC	1208	0.20283566952561974	No Hit
ACACCCGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAG	1173	0.19695880823969533	No Hit
ACACCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1171	0.19662298759478536	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1144	0.19208940888850085	No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1123	0.18856329211694617	No Hit
ACACCCCGTTTCCCGGCTTCTCAATGCCTCGTGATGGTATATGTTTAACA	1112	0.18671627856994136	No Hit
ACACCCGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC	1092	0.1833580721208417	No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1060	0.17798494180228222	No Hit
ACACCCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1039	0.17445882503072757	No Hit
ACACCCGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTC	1032	0.1732834527735427	No Hit
ACACCCGGAGGCCCAGTGCAGTTCTCTGGAGAAAGCTACCTTTTCCCAAG	1026	0.17227599083881281	No Hit
ACACCCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1017	0.17076479793671798	No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	1000	0.16791032245498325	No Hit
ACACCCGGGATTGAAAAAGGGTCTTTGTTTAGCCCAGGCTGGCCTCATAC	941	0.15800361343013922	No Hit
ACACCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG	889	0.1492722766624801	No Hit
ACACCCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA	872	0.14641780118074538	No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGT	867	0.14557824956847049	No Hit
ACACCCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	858	0.14406705666637562	No Hit
ACACCCGGGCAAATGTAAGAAGTCCCTCACATCCGATTAGCCCAACTCTG	854	0.14339541537655567	No Hit
ACACCCGGGATGGGATTCATTGGCTTCTTCGTGAAACTGATCCATATACC	814	0.13667900247835635	No Hit
ACACCCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA	804	0.1349998992538065	No Hit
ACACCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	777	0.13046632054752197	No Hit
ACACCCGGGGAACTAAAGTAACTCATATTTATGTAGTAGAATGCTTATGT	772	0.12962676893524705	No Hit
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	740	0.1242536386166876	No Hit
ACACCCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC	725	0.12173498377986286	No Hit
ACACCCGGGATTGTCAATTTTTCTGACTTTTTAAATCATCATCATTATTT	723	0.12139916313495289	No Hit
ACACCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT	721	0.12106334249004291	No Hit
ACACCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	712	0.11955214958794806	No Hit
ACACCCGGGATGGGATTCAGGTTGAGCCCCCAAACTGTGAATTCAGTTGC	701	0.11770513604094325	No Hit
ACACCCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	689	0.11569021217148347	No Hit
ACACCCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	681	0.11434692959184359	No Hit
ACACCCGGGGGCCGAGCTCGTCGCTAGGTTTGTTGAAGGACATCGGCCGC	666	0.11182827475501883	No Hit
ACACCCGGCAGTGTCATTCCTATGAGGCCTGTGCCAAGTTGGTGATGGAT	651	0.10930961991819409	No Hit
ACACCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	650	0.1091417095957391	No Hit
ACACCCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGT	641	0.10763051669364426	No Hit
ACACCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCT	640	0.10746260637118928	No Hit
ACACCCGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	639	0.10729469604873429	No Hit
ACACCCGGGATTCCTGATGGCTGTGGTGTCAAATATATCCCCAATCGTGG	633	0.1062872341140044	No Hit
ACACCCGGGGACACCAAACTGGTTCTTGAACCTCTCGGCCCCGATTCTGA	613	0.10292902766490472	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.037309673649497E-4	0.0	0.0	0.0	0.0
8	8.395516122749162E-4	0.0	0.0	0.0	0.0
9	0.0011753722571848826	0.0	0.0	0.0	0.0
10	0.00470148902873953	0.0	0.0	0.0	0.0
11	0.010242529669753978	0.0	0.0	0.0	0.0
12	0.014272377408673575	0.0	0.0	0.0	0.0
13	0.03425370578081658	0.0	0.0	0.0	0.0
14	0.055578316732599456	0.0	0.0	0.0	0.0
15	0.06884323220654313	0.0	0.0	0.0	0.0
16	0.0891603812235961	0.0	0.0	0.0	0.0
17	0.09570888379934045	0.0	0.0	0.0	0.0
18	0.10057828315053496	0.0	0.0	0.0	0.0
19	0.1062872341140044	0.0	0.0	0.0	0.0
20	0.1121640953999288	0.0	0.0	0.0	0.0
21	0.13466407860889656	0.0	0.0	0.0	0.0
22	0.13701482312326632	0.0	0.0	0.0	0.0
23	0.14473869795619557	0.0	0.0	0.0	0.0
24	0.1563245102055894	0.0	0.0	0.0	0.0
25	0.16253719213642379	0.0	0.0	0.0	0.0
26	0.16807823277743822	0.0	0.0	0.0	0.0
27	0.17496255599809254	0.0	0.0	0.0	0.0
28	0.24195877465763085	0.0	0.0	0.0	0.0
29	0.2474998152986453	0.0	0.0	0.0	0.0
30	0.26580204044623845	0.0	0.0	0.0	0.0
31	0.2715109914097079	0.0	0.0	0.0	0.0
32	0.27906695592018216	0.0	0.0	0.0	0.0
33	0.28511172752856156	0.0	0.0	0.0	0.0
34	0.29434679526358565	0.0	0.0	0.0	0.0
35	0.3544586907024696	0.0	0.0	0.0	0.0
36	0.36436539972731363	0.0	0.0	0.0	0.0
37	0.36940270940096315	0.0	0.0	0.0	0.0
38	0.37628703262161745	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.853421E-4	44.000004	41
TATTTCG	20	7.853421E-4	44.000004	42
AGCGTAC	45	4.783942E-10	44.000004	10
TCGTTTA	20	7.853421E-4	44.000004	18
GATACTC	20	7.853421E-4	44.000004	10
AGGTACA	20	7.853421E-4	44.000004	42
ACTATAT	20	7.853421E-4	44.000004	25
TCGTTAA	20	7.853421E-4	44.000004	16
ATCTCCG	20	7.853421E-4	44.000004	25
CGACGGG	20	7.853421E-4	44.000004	36
CATGCGC	45	4.783942E-10	44.000004	14
TAGACGG	20	7.853421E-4	44.000004	24
GCGAAAT	20	7.853421E-4	44.000004	28
CATGATA	20	7.853421E-4	44.000004	40
CATGACG	20	7.853421E-4	44.000004	34
CATTGCG	20	7.853421E-4	44.000004	24
TTAGACG	20	7.853421E-4	44.000004	23
CGGTTAT	20	7.853421E-4	44.000004	14
TTATGCC	20	7.853421E-4	44.000004	23
CCAATAC	20	7.853421E-4	44.000004	38
>>END_MODULE