FastQCFastQC Report
Fri 17 Jun 2016
SRR1527218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642959
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGACCACATTTCTACACTTTTAATACGTCAGTGTCCATGTTAAGT27220.4233551439516361No Hit
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG26730.41573412923685643No Hit
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT25180.3916268377921454No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGAAG24180.37607374653749304No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA23810.3703191027732717No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT23050.35849875341973597No Hit
ACACCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG19910.30966204688012766No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC19390.30157443942770845No Hit
ACACCCGGGGTCCTTTACTTTCCCTCCTGACAGATAGTCTAGAAGCCGATGT18480.28742112638597483No Hit
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG14070.2188319939529581No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC14020.2180543393902255No Hit
ACACCCCGTTTCCCGGCTTCTCAATGCCTCGTGATGGTATATGTTTAACAGT13510.21012226285035282No Hit
ACACCCGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGC12750.19830191349681706No Hit
ACACCCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC11230.17466121478974553No Hit
ACACCCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT10920.16983975650080332No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10560.1642406436491285No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC10490.16315192726130281No Hit
ACACCCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC10430.1622187417860237No Hit
ACACCCGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCC10390.1615966181358376No Hit
ACACCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC10220.1589525926225467No Hit
ACACCCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG10220.1589525926225467No Hit
ACACCCGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCT10180.15833046897236058No Hit
ACACCCGGGATTGAAAAAGGGTCTTTGTTTAGCCCAGGCTGGCCTCATACTT10090.1569306907594419No Hit
ACACCCGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT10050.15630856710925578No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA9600.14930967604466225No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC9090.1413775995047896No Hit
ACACCCGGAGGCCCAGTGCAGTTCTCTGGAGAAAGCTACCTTTTCCCAAGGC9050.1407554758546035No Hit
ACACCCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8710.13546742482802168No Hit
ACACCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA8670.1348453011778356No Hit
ACACCCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT8260.12846853376342815No Hit
ACACCCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA8160.12691322463796292No Hit
ACACCCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA7760.12069198813610199No Hit
ACACCCGGGATTGTCAATTTTTCTGACTTTTTAAATCATCATCATTATTTTT7740.12038092631100894No Hit
ACACCCGGGGAACTAAAGTAACTCATATTTATGTAGTAGAATGCTTATGTTT7710.11991433357336938No Hit
ACACCCGGGATGGGATTCATTGGCTTCTTCGTGAAACTGATCCATATACCCA7590.11804796262281109No Hit
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC7400.11509287528442716No Hit
ACACCCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC7390.11493734437188062No Hit
ACACCCGGCAGTGTCATTCCTATGAGGCCTGTGCCAAGTTGGTGATGGATAG7060.10980482425784538No Hit
ACACCCGGGCAAATGTAAGAAGTCCCTCACATCCGATTAGCCCAACTCTGTC6940.10793845330728709No Hit
ACACCCGGGATGGGATTCAGGTTGAGCCCCCAAACTGTGAATTCAGTTGCAA6920.10762739148219404No Hit
ACACCCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC6840.10638314418182186No Hit
ACACCCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT6830.10622761326927532No Hit
ACACCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA6740.10482783505635662No Hit
ACACCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG6740.10482783505635662No Hit
ACACCCGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG6640.1032725259308914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAT700.046.00000438
AACGTGT351.0186341E-746.00000439
ATGGGGC700.046.00000443
CTAGACA551.8189894E-1246.00000417
GATACCT351.0186341E-746.00000431
CGAGTAG351.0186341E-746.00000424
CGAAACT351.0186341E-746.00000429
CACGGAA351.0186341E-746.00000415
TTCGTAA351.0186341E-746.00000430
AAACGTG351.0186341E-746.00000438
AAACGTC351.0186341E-746.00000437
TACGCGC551.8189894E-1246.00000412
TACTGAT351.0186341E-746.00000422
CAAATTC351.0186341E-746.00000435
TCCGTCT351.0186341E-746.00000415
GTATACC351.0186341E-746.00000423
TGGTACG351.0186341E-746.00000413
TCACGAC501.6370905E-1146.045
AGCGTAC206.3093693E-446.010
TAATACG3050.046.028
Produced by FastQC (version 0.11.5)