##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527218_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	642959
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28926261239052	33.0	31.0	34.0	31.0	34.0
2	32.60582712116947	34.0	31.0	34.0	31.0	34.0
3	32.62927496154498	34.0	31.0	34.0	31.0	34.0
4	36.33542418723433	37.0	37.0	37.0	35.0	37.0
5	36.33576479993281	37.0	37.0	37.0	35.0	37.0
6	36.40193542667573	37.0	37.0	37.0	35.0	37.0
7	36.49057871497249	37.0	37.0	37.0	35.0	37.0
8	36.57771179810843	37.0	37.0	37.0	35.0	37.0
9	38.67162136310402	39.0	39.0	39.0	38.0	39.0
10	38.057053404649444	39.0	38.0	39.0	37.0	39.0
11	37.831992397648996	39.0	38.0	39.0	35.0	39.0
12	37.27817947956246	39.0	37.0	39.0	35.0	39.0
13	37.1664989524993	39.0	37.0	39.0	34.0	39.0
14	38.01974775996603	40.0	38.0	40.0	34.0	40.0
15	37.92967358727384	40.0	38.0	40.0	33.0	40.0
16	32.83962429952765	40.0	34.0	40.0	3.0	40.0
17	34.451591781124456	40.0	34.0	40.0	16.0	40.0
18	36.45560447866816	40.0	34.0	40.0	30.0	40.0
19	37.242483579823904	39.0	36.0	40.0	32.0	40.0
20	37.58326891761372	39.0	37.0	40.0	33.0	40.0
21	37.80699391407539	40.0	37.0	40.0	33.0	40.0
22	37.82468711068668	40.0	37.0	40.0	34.0	40.0
23	37.78501584082344	40.0	37.0	40.0	34.0	40.0
24	37.75557694969664	40.0	37.0	40.0	34.0	40.0
25	37.64803976614372	40.0	36.0	40.0	33.0	40.0
26	37.5176908636476	40.0	36.0	40.0	33.0	40.0
27	37.35413144539543	40.0	36.0	40.0	33.0	40.0
28	37.21218304744159	39.0	35.0	40.0	33.0	40.0
29	36.95231888814061	39.0	35.0	40.0	32.0	40.0
30	36.86069251694121	39.0	35.0	40.0	32.0	40.0
31	36.740020747823735	39.0	35.0	40.0	32.0	40.0
32	36.44312156762717	38.0	35.0	40.0	31.0	40.0
33	36.15246228764198	38.0	35.0	40.0	31.0	40.0
34	36.0402980594408	38.0	35.0	40.0	30.0	40.0
35	35.93567552518901	38.0	35.0	40.0	30.0	40.0
36	35.73671260531387	38.0	35.0	40.0	30.0	40.0
37	35.27782953500923	37.0	34.0	40.0	29.0	40.0
38	35.21517701750812	37.0	34.0	40.0	29.0	40.0
39	34.874931371984836	36.0	34.0	40.0	28.0	40.0
40	34.80052693873171	36.0	34.0	40.0	27.0	40.0
41	34.747629320065506	36.0	34.0	40.0	28.0	40.0
42	34.6290012271389	35.0	33.0	39.0	27.0	40.0
43	34.54680780578544	35.0	33.0	39.0	28.0	40.0
44	34.752175488639246	35.0	34.0	39.0	28.0	40.0
45	34.77367608198968	35.0	34.0	39.0	29.0	40.0
46	34.719675749153524	35.0	34.0	39.0	29.0	40.0
47	34.399336505127074	35.0	34.0	39.0	28.0	40.0
48	34.34036695963506	35.0	34.0	39.0	28.0	40.0
49	34.22774235993275	35.0	34.0	39.0	28.0	40.0
50	33.63055342564611	35.0	33.0	38.0	26.0	40.0
51	33.75165290477309	35.0	33.0	37.0	26.0	40.0
52	33.3843744313401	35.0	33.0	37.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	5.0
15	9.0
16	18.0
17	41.0
18	86.0
19	174.0
20	344.0
21	626.0
22	1061.0
23	1653.0
24	2471.0
25	3621.0
26	5174.0
27	6900.0
28	8174.0
29	10256.0
30	12953.0
31	16177.0
32	22429.0
33	32787.0
34	51224.0
35	70554.0
36	97291.0
37	123718.0
38	123680.0
39	51532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	98.44562406001005	0.5790415874107059	0.20934460828762022	0.7659897442916267
8	99.09496561989178	0.39349320874270366	0.19363598612042135	0.3179051852450934
9	97.12081174693876	1.5175151137164267	0.7569689513639283	0.6047041879808822
10	60.95489759067063	27.37826206647702	5.454469103006568	6.212371239845775
11	37.56740320922485	24.238559534900357	18.98130362900278	19.21273362687201
12	30.56244643904199	14.034487424548066	28.249079645825002	27.153986490584934
13	23.75221437136738	16.329657100997107	28.757821260764683	31.16030726687083
14	23.81224930361034	19.080687882120014	28.942903046695044	28.164159767574603
15	25.813621086258998	19.174006429647925	28.495129549473607	26.51724293461947
16	28.65859253856	25.252465553791144	25.342206890330488	20.746735017318368
17	31.980266237816096	18.045007535472713	22.020377660161845	27.954348566549342
18	30.671629139649653	18.787201050144724	23.070677912588515	27.470491897617112
19	30.75608242516241	17.731768277604015	23.745837603952975	27.7663116932806
20	29.795523509275085	21.215660718646134	24.27790885577463	24.71090691630415
21	30.20565852566027	17.370469967758442	26.9228675545408	25.501003952040485
22	29.139338589241305	15.818271460544139	25.759496328692812	29.282893621521744
23	27.742204401835885	22.187573391149357	23.661228787527666	26.408993419487093
24	28.37957008145154	21.162002553817583	23.404913843650995	27.053513521079882
25	28.185467502593475	20.351530968537652	24.36376191950031	27.09923960936856
26	30.587953508699623	19.21226703413437	22.116651295028145	28.083128162137864
27	27.242172517998814	17.09735768532675	23.637899150645687	32.022570646028754
28	27.707832070163107	20.156961796941953	23.57646444018981	28.558741692705137
29	31.511185005575783	19.677304462648472	21.553318329784638	27.258192201991104
30	26.45067570404956	21.044421183932414	23.260892218632915	29.244010893385113
31	31.79176277180971	19.932375159224772	22.02862079852681	26.247241270438703
32	30.540205518547843	20.641285058611825	22.91343616000398	25.905073262836353
33	34.64357758426276	18.376910502846993	21.35719384906347	25.622318063826775
34	30.422313086837576	22.00809071807067	22.624615255405086	24.944980939686666
35	30.674117634250397	23.13708961224588	24.805469711132435	21.383323042371284
36	32.315746416178946	21.984294488451052	21.830785477767634	23.869173617602364
37	30.517342474403502	22.632391801032416	21.202285060167135	25.647980664396954
38	34.07464550616758	21.520812369062416	21.991293379515646	22.413248745254364
39	30.530407071057407	20.549988412947016	20.88049160210838	28.039112913887198
40	27.74795904560011	22.47779407396117	24.042901646916835	25.73134523352189
41	26.80404193735526	22.546538737306733	22.501279241755697	28.148140083582312
42	27.07668762704931	19.72707435466336	24.027193024749636	29.16904499353769
43	25.708948782115186	20.825589189979453	25.66679990481508	27.798662123090274
44	25.98408296640999	19.846677626411637	25.714392364054316	28.454847043124055
45	26.677284243629845	19.062179703526976	25.195074647061478	29.065461405781708
46	28.877424532512958	18.441144769728705	23.783476084789232	28.8979546129691
47	28.354529604531546	20.991696204579142	23.953937964940224	26.699836225949085
48	30.876463351473422	20.209997838120316	24.515715621058263	24.397823189348
49	29.095012279165545	22.305310291947077	23.715509076006402	24.884168352880977
50	25.944267052798082	22.295978437194282	25.181698988582475	26.57805552142516
51	28.154361320084178	21.801234604383797	23.281111237263964	26.76329283826807
52	28.191844270007888	21.249099864843636	25.207361589152654	25.351694275995822
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	4.5
10	6.0
11	17.0
12	28.0
13	129.0
14	318.0
15	406.0
16	482.0
17	558.0
18	651.5
19	745.0
20	758.5
21	772.0
22	727.0
23	682.0
24	826.0
25	970.0
26	1075.0
27	1180.0
28	1987.5
29	2795.0
30	2357.0
31	1919.0
32	3357.5
33	4796.0
34	5099.5
35	5403.0
36	5102.0
37	4801.0
38	5695.0
39	7320.0
40	8051.0
41	10071.0
42	12091.0
43	11695.5
44	11300.0
45	12290.0
46	13280.0
47	17995.5
48	22711.0
49	20056.5
50	17402.0
51	20837.0
52	24272.0
53	24299.5
54	24327.0
55	27866.5
56	31406.0
57	35304.5
58	39203.0
59	48604.5
60	58006.0
61	61597.5
62	65189.0
63	62594.0
64	58604.0
65	57209.0
66	51920.0
67	46631.0
68	41560.0
69	36489.0
70	30324.0
71	24159.0
72	22472.0
73	20785.0
74	18799.5
75	16814.0
76	15251.0
77	13688.0
78	9177.0
79	4666.0
80	3434.5
81	2203.0
82	1372.5
83	542.0
84	536.0
85	530.0
86	318.0
87	106.0
88	59.0
89	7.0
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	642959.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.516011129792103
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.71885312647323	10.120396479402263
2	11.304804354347919	3.2820133165567325
3	4.796854240774868	2.088935686412353
4	2.4986071229588998	1.4507923522339683
5	1.5493078472549608	1.1244884977113627
6	1.0575151073586764	0.9210540641005103
7	0.7232246174945356	0.7348835617823221
8	0.6021514593065616	0.6992669828091682
9	0.4628637551965028	0.6047041879808822
>10	4.106844383491193	13.572560614284892
>50	1.609308704410063	16.88070312414944
>100	1.4989499849997856	38.312396280322695
>500	0.045000642866326664	4.3430452019491135
>1k	0.02571465306647238	5.864759650304297
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACACCCGGGACCACATTTCTACACTTTTAATACGTCAGTGTCCATGTTAAGT	2722	0.4233551439516361	No Hit
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2673	0.41573412923685643	No Hit
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2518	0.3916268377921454	No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGAAG	2418	0.37607374653749304	No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2381	0.3703191027732717	No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2305	0.35849875341973597	No Hit
ACACCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1991	0.30966204688012766	No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1939	0.30157443942770845	No Hit
ACACCCGGGGTCCTTTACTTTCCCTCCTGACAGATAGTCTAGAAGCCGATGT	1848	0.28742112638597483	No Hit
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1407	0.2188319939529581	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1402	0.2180543393902255	No Hit
ACACCCCGTTTCCCGGCTTCTCAATGCCTCGTGATGGTATATGTTTAACAGT	1351	0.21012226285035282	No Hit
ACACCCGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGC	1275	0.19830191349681706	No Hit
ACACCCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	1123	0.17466121478974553	No Hit
ACACCCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT	1092	0.16983975650080332	No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1056	0.1642406436491285	No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1049	0.16315192726130281	No Hit
ACACCCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC	1043	0.1622187417860237	No Hit
ACACCCGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCC	1039	0.1615966181358376	No Hit
ACACCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1022	0.1589525926225467	No Hit
ACACCCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1022	0.1589525926225467	No Hit
ACACCCGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCT	1018	0.15833046897236058	No Hit
ACACCCGGGATTGAAAAAGGGTCTTTGTTTAGCCCAGGCTGGCCTCATACTT	1009	0.1569306907594419	No Hit
ACACCCGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT	1005	0.15630856710925578	No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	960	0.14930967604466225	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	909	0.1413775995047896	No Hit
ACACCCGGAGGCCCAGTGCAGTTCTCTGGAGAAAGCTACCTTTTCCCAAGGC	905	0.1407554758546035	No Hit
ACACCCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	871	0.13546742482802168	No Hit
ACACCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	867	0.1348453011778356	No Hit
ACACCCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	826	0.12846853376342815	No Hit
ACACCCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA	816	0.12691322463796292	No Hit
ACACCCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA	776	0.12069198813610199	No Hit
ACACCCGGGATTGTCAATTTTTCTGACTTTTTAAATCATCATCATTATTTTT	774	0.12038092631100894	No Hit
ACACCCGGGGAACTAAAGTAACTCATATTTATGTAGTAGAATGCTTATGTTT	771	0.11991433357336938	No Hit
ACACCCGGGATGGGATTCATTGGCTTCTTCGTGAAACTGATCCATATACCCA	759	0.11804796262281109	No Hit
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	740	0.11509287528442716	No Hit
ACACCCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	739	0.11493734437188062	No Hit
ACACCCGGCAGTGTCATTCCTATGAGGCCTGTGCCAAGTTGGTGATGGATAG	706	0.10980482425784538	No Hit
ACACCCGGGCAAATGTAAGAAGTCCCTCACATCCGATTAGCCCAACTCTGTC	694	0.10793845330728709	No Hit
ACACCCGGGATGGGATTCAGGTTGAGCCCCCAAACTGTGAATTCAGTTGCAA	692	0.10762739148219404	No Hit
ACACCCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	684	0.10638314418182186	No Hit
ACACCCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT	683	0.10622761326927532	No Hit
ACACCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA	674	0.10482783505635662	No Hit
ACACCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	674	0.10482783505635662	No Hit
ACACCCGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG	664	0.1032725259308914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.1106182509304636E-4	0.0	0.0	0.0	0.0
8	0.003110618250930464	0.0	0.0	0.0	0.0
9	0.003266149163476987	0.0	0.0	0.0	0.0
10	0.0068433601520470205	0.0	0.0	0.0	0.0
11	0.013064596653907948	0.0	0.0	0.0	0.0
12	0.016486276729931457	0.0	0.0	0.0	0.0
13	0.03748294992371209	0.0	0.0	0.0	0.0
14	0.07465483802233112	0.0	0.0	0.0	0.0
15	0.08367563095002947	0.0	0.0	0.0	0.0
16	0.10498336596890315	0.0	0.0	0.0	0.0
17	0.11027141699548494	0.0	0.0	0.0	0.0
18	0.1154039371095202	0.0	0.0	0.0	0.0
19	0.12178070452392765	0.0	0.0	0.0	0.0
20	0.13002384288889338	0.0	0.0	0.0	0.0
21	0.1496207378697553	0.0	0.0	0.0	0.0
22	0.154286665246151	0.0	0.0	0.0	0.0
23	0.16190767996093064	0.0	0.0	0.0	0.0
24	0.1703063492384429	0.0	0.0	0.0	0.0
25	0.1731059056642803	0.0	0.0	0.0	0.0
26	0.1797937349037808	0.0	0.0	0.0	0.0
27	0.18632603323073477	0.0	0.0	0.0	0.0
28	0.2454277799984136	0.0	0.0	0.0	0.0
29	0.25056030011244884	0.0	0.0	0.0	0.0
30	0.27404546790697387	0.0	0.0	0.0	0.0
31	0.2802667044088348	0.0	0.0	0.0	0.0
32	0.2889764355114401	0.0	0.0	0.0	0.0
33	0.2937978938003823	0.0	0.0	0.0	0.0
34	0.30344081037826676	0.0	0.0	0.0	0.0
35	0.3549215424311659	0.0	0.0	0.0	0.0
36	0.3662752990470621	0.0	0.0	0.0	0.0
37	0.37187441189873693	0.0	0.0	0.0	0.0
38	0.37871777205078394	0.0	0.0	0.0	0.0
39	0.38478347764009835	0.0	0.0	0.0	0.0
40	0.3931821469176106	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTAT	70	0.0	46.000004	38
AACGTGT	35	1.0186341E-7	46.000004	39
ATGGGGC	70	0.0	46.000004	43
CTAGACA	55	1.8189894E-12	46.000004	17
GATACCT	35	1.0186341E-7	46.000004	31
CGAGTAG	35	1.0186341E-7	46.000004	24
CGAAACT	35	1.0186341E-7	46.000004	29
CACGGAA	35	1.0186341E-7	46.000004	15
TTCGTAA	35	1.0186341E-7	46.000004	30
AAACGTG	35	1.0186341E-7	46.000004	38
AAACGTC	35	1.0186341E-7	46.000004	37
TACGCGC	55	1.8189894E-12	46.000004	12
TACTGAT	35	1.0186341E-7	46.000004	22
CAAATTC	35	1.0186341E-7	46.000004	35
TCCGTCT	35	1.0186341E-7	46.000004	15
GTATACC	35	1.0186341E-7	46.000004	23
TGGTACG	35	1.0186341E-7	46.000004	13
TCACGAC	50	1.6370905E-11	46.0	45
AGCGTAC	20	6.3093693E-4	46.0	10
TAATACG	305	0.0	46.0	28
>>END_MODULE