FastQCFastQC Report
Fri 17 Jun 2016
SRR1527217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624582
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGACCACATTTCTACACTTTTAATACGTCAGTGTCCATGTTAAGT26980.43196890080085565No Hit
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG26250.4202810839889718No Hit
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT25130.402349091072109No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGAAG24200.3874591326679283No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT23940.3832963485979423No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA22820.3653643556810795No Hit
ACACCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG19020.30452366542743786No Hit
ACACCCGGGGTCCTTTACTTTCCCTCCTGACAGATAGTCTAGAAGCCGATGT18770.3005209884370667No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC17430.2790666397686773No Hit
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG14770.23647815659112817No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC14720.2356776211930539No Hit
ACACCCGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGC12370.198052457483565No Hit
ACACCCCGTTTCCCGGCTTCTCAATGCCTCGTGATGGTATATGTTTAACAGT12120.19404978049319385No Hit
ACACCCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC11400.1825220707609249No Hit
ACACCCGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCT11010.1762778946559459No Hit
ACACCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC10810.17307575306364897No Hit
ACACCCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT10660.17067414686942628No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC10610.16987361147135202No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10210.16346932828675817No Hit
ACACCCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC10100.16170815041099487No Hit
ACACCCGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT9700.15530386722640102No Hit
ACACCCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG9560.15306236811179316No Hit
ACACCCGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCC9530.15258204687294863No Hit
ACACCCGGAGGCCCAGTGCAGTTCTCTGGAGAAAGCTACCTTTTCCCAAGGC9460.1514612973156447No Hit
ACACCCGGGATTGAAAAAGGGTCTTTGTTTAGCCCAGGCTGGCCTCATACTT9110.14585754952912508No Hit
ACACCCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8720.13961337342414606No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC8610.13785219554838277No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA8390.13432983979685614No Hit
ACACCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA8340.1335293043987819No Hit
ACACCCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA8300.13288887608032252No Hit
ACACCCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA7940.12712502121418806No Hit
ACACCCGGGATGGGATTCATTGGCTTCTTCGTGAAACTGATCCATATACCCA7860.12584416457726927No Hit
ACACCCGGGCAAATGTAAGAAGTCCCTCACATCCGATTAGCCCAACTCTGTC7600.12168138050728328No Hit
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC7570.12120105926843874No Hit
ACACCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA7390.1183191318353715No Hit
ACACCCGGGGAACTAAAGTAACTCATATTTATGTAGTAGAATGCTTATGTTT7030.11255527696923703No Hit
ACACCCGGGATGGGATTCAGGTTGAGCCCCCAAACTGTGAATTCAGTTGCAA7020.11239516988962217No Hit
ACACCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG6970.11159463449154794No Hit
ACACCCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT6950.11127442033231824No Hit
ACACCCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC6670.10679142210310257No Hit
ACACCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT6500.10406960174965017No Hit
ACACCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC6460.10342917343119079No Hit
ACACCCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC6390.10230842387388686No Hit
ACACCCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT6340.10150788847581263No Hit
ACACCCGGGATTGTCAATTTTTCTGACTTTTTAAATCATCATCATTATTTTT6270.10038713891850871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTTA551.8189894E-1246.00000433
CGAAACT551.8189894E-1246.00000429
AAACGTG351.0185613E-746.00000438
CCTCATA1150.046.00000443
TACTGAT351.0185613E-746.00000422
CTCATAC1150.046.00000444
TACTAAC1150.046.00000439
TCTATTG351.0185613E-746.00000421
TACCGAA351.0185613E-746.00000441
TATCCAT351.0185613E-746.00000439
TCACGAC301.8600276E-646.045
CTAGACA253.4153523E-546.017
TCGTTTA206.3092296E-446.018
TAATACG3300.046.028
TAATACC301.8600276E-646.045
CAAACTA253.4153523E-546.043
ATTAGTG253.4153523E-546.043
ATGATAC301.8600276E-646.028
GTATTAT206.3092296E-446.039
CAACGAA206.3092296E-446.015
Produced by FastQC (version 0.11.5)