FastQCFastQC Report
Fri 17 Jun 2016
SRR1527216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22258
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC6592.960733219516578No Hit
CGGCAGGGGGAGCGCACAGAGCGCGGTGACGATGAAGCCCCAATGCAGGC6342.848414053374068No Hit
CGGCAGGGGGTTCCGGCATGGCGACCTCGGCGTTTGTCGCGTACCTGTCG4732.1250786234162997No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGA4562.0487015904393924No Hit
CGGCAGGGGGTTCAATCGCTCGATGGCCGCCTTGTTGGTGGCCAGCGTGG4171.873483691257076No Hit
CGGCAGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3731.6758019588462576No Hit
CGGCAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA3731.6758019588462576No Hit
CGGCAGGGGGAAGCCAGATATCGCATCGACTCCCTCGCGAAGCAGGAAGC2891.298409560607422No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2391.073771228322401No Hit
CGGCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG2371.0647856950310002No Hit
CGGCAGGGGAGAGTGCAGTGCTCGGCAGGGGGCTCAACGGCTACCAGGTG2281.0243507952196962No Hit
CGGCAGCGGCGCCACCATCACCTCCTGGCCTTCCTTGATCAGCGGATGGC2140.9614520621798903No Hit
CGGCAGGGGGCCGAAGCGCAGCACCACGGCTTACTCCTGCTCGTCCACCA2120.9524665288884895No Hit
CGGCAGGGGTGGTCCACGACCTGCGGGTGCAGTTGTTCAACAACCTGCTG2050.9210171623685867No Hit
CGGCAGGGTCATTGAAGGTATGCACCTCACTGGCTGCAGACTTCTGCAAC2050.9210171623685867No Hit
CGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACG1990.894060562494384No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGC1950.8760894959115824No Hit
CGGCAGGGGTTCCCTGGTGGTCCAGTAGTTAGGACCTGGCACTTTCACTG1920.862611195974481No Hit
CGGCAGGGGTTTCATCGCTGTTAAACTGGGCGCACATTTGATTGATTTTT1880.8446401293916794No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.840147362745979No Hit
CGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAGAAGTCCTTTACCAGCT1810.8131907628717764No Hit
CGGCAGGGGTGGGTGGTCAGGTCCAGGTTTGCGCTGCGCAGGCCCTGGAA1780.7997124629346751No Hit
CGGCAGGGGATCTGGGTAGCGCGTTCGGCGCTGTAGAGGATCACGCCGGC1740.7817413963518735No Hit
CGGCAGGGGCGGTGCAATGGCTTTACGAAAGTAATCCGCTACTGGCGGAA1720.7727558630604726No Hit
CGGCAGAAGCCTGAATGAGCTTAATAGAGGCCAAAGCGGTCTGGAAACGT1660.7457992631862701No Hit
CGGCAGGGGCTAGGTGTTTTCTGGATAGCTCGGCGCTGCCCCTCATCCGC1650.7413064965405697No Hit
CGGCAGGGGGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGT1620.7278281966034684No Hit
CGGCAGGGGGCCATGAAATTAAAAGACGCTTACTCCTTGGAAGGAAAGTT1590.7143498966663672No Hit
CGGCAGGGGGGGCACGGTGGCTCACACCTGTAAGCCCAGCACTTTGGGAG1590.7143498966663672No Hit
CGGCAGGGGCAATGCCCTGCAATAAAGAAGCTCCATCGGCACCTCCTATC1540.691886063437865No Hit
CGGCAGGGGGAGTGCAGTGCTCGGCAGGGGACCCCATCTTTACCAAAAAT1540.691886063437865No Hit
CGGCAGGGAGGCGGGGATCTGCAGGTGCAAGCAGAAATCATCAAGCAAAG1460.6559439302722616No Hit
CGGCAGCAATAAACTCAACAGGAGCAGGAAAGCGAGGGTATCCTACAAAG1380.6200017971066583No Hit
CGGCAGGGGGTGCAGTGCTCGGCAGGGGGACTTTTTTTTTTTTTTTTTTT1350.606523497169557No Hit
CGGCAGATTGCGATAAACGGTCACATTAAATTTAACCTGACTATTCCACT1330.5975379638781562No Hit
CGGCAGGGGCAACGTGAGTGCCTGTTTTTGAGTCGTTGAGTTCTACAGTC1250.5615958307125528No Hit
CGGCAGGGGGCATCTAGACTGACTGAAAAAATGCCGTGCGAAAAAACATG1250.5615958307125528No Hit
CGGCAGGGGGGTGTGCACCATTGCTCACTATTTGACCACCGCCCAAATGA1230.552610297421152No Hit
CGGCAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.5121753976098481No Hit
CGGCAGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTT1020.4582621978614431No Hit
CGGCAGGGGGGACGGCGTCATGGCCGCCGAAATGGCGCGTTCGGGCTTCA990.4447838979243418No Hit
CGGCAGGGGACGCGTGGCGATGGACAGGCGGCGTCCATCCTTTGAGGGAA980.4402911312786414No Hit
CGGCAGGGGTTCCCGGGCGCTTTCGCGCTGACGCGCGAAGAAGCGGCTTC970.43579836463294097No Hit
CGGCAGGGGGCGTTTCTCCTTCCTGCCACCTTGTGAAGGTGGGTGCCTTG940.4223200646958397No Hit
CGGCAGGGGCCCTCCAGCCAGGATGCTTAATGGGCAAACCCAGTTCAGTG910.40884176475873846No Hit
CGGCAGGGGGAGTGCAGTGCTTTGCAGGGGTGCACCTATGCGCTGACCAT910.40884176475873846No Hit
CGGCAGGGGAGAGTAACTTTCACTATACACCTATCGGAATTTTTTAAATA900.404348998113038No Hit
CGGCAGGGGGACTTATCGATAAGATTTCCGCCACTGTACTTTTTGTAAGC900.404348998113038No Hit
CGGCAGGGGGTGCTCGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA870.3908706981759367No Hit
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.3863779315302363No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGG850.3818851648845359No Hit
CGGCAGGGGTTGCCGGATGGCCTGTTCAACGACGAAACCGACGGGCATGT800.3594213316560338No Hit
CGGCAGGGGAGAACGGCGAGCAGGCCGGGAATGAGCGTCAGCCAGAAGAC800.3594213316560338No Hit
CGGCAGGGGAGTGGTATCAAGTGCAGTGCTCGGCAGGGGTAAAAAAAAAA770.3459430317189325No Hit
CGGCAGGGGAGCCTTCTCGCACCACTCCGCCGCCGCGCTGGCCGCTGCCT760.3414502650732321No Hit
CGGCAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGCAGCGCC730.3279719651361308No Hit
CGGCAGCAAGAACCATACGACCAATATCACGAAAATAGTCACGCAAAGCA730.3279719651361308No Hit
CGGCAGGGGAGAGTAATTTATTTGCAGTCAGTTGATTATGTACAGAGTTT660.2965225986162279No Hit
CGGCAGGGGGCGTGGGCGTCCGGCCGCAATGGCCAGCGGCTGGCTCAGGC660.2965225986162279No Hit
CGGCAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.29202983197052745No Hit
CGGCAGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.28753706532482703No Hit
CGGCAGGGGGATGCAGAAAAGGTCTTTGACAAAATTCAACAATCCTTCAT630.2830442986791266No Hit
CGGCAGGGGGGTCCGAGGGGCCGTGGGCCGGGGCGTGTGAGGAGACGCGG610.2740587653877258No Hit
CGGCAGGGGGCCACAAGCCTGGTGCCCAAGGAAAAAGCCGCCAGCGCGAT610.2740587653877258No Hit
CGGCAGGGGTGCTCGGCAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCC590.26507323209632494No Hit
CGGCAGGGGTTTAGTCCTTTTTATTCACTTCAGTCTCTTCAGCTCGGTTC590.26507323209632494No Hit
CGGCAGGGGGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.26507323209632494No Hit
CGGCAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.25608769880492405No Hit
CGGCAGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.2515949321592236No Hit
CGGCAGGGGGGCTGGCTCGGGGCAGCCGGGCGCGAGCGGCGTAGACAAGG550.24710216551352324No Hit
CGGCAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.23362386557642195No Hit
CGGCAGGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA510.22913109893072156No Hit
CGGCAGGGGAGGCAGTGGCAAGTCCCGGATGTTGGCCGCCAGTGGCTTGC500.22463833228502111No Hit
CGGCAGGGGGTGCTCGGCAGGGGCTGGTGATACGGAAGATGTCCATCCAG480.21565279899362025No Hit
CGGCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.21116003234791986No Hit
CGGCAGGGGAAGCACTGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGA420.18869619911941773No Hit
CGGCAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.18420343247371732No Hit
CGGCAGGGGCTGGCCAACTTTGAAAAGGCCACCGGCATCAAGCCGGTCTA400.1797106658280169No Hit
CGGCAGGGGACGCAGAGTCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.1797106658280169No Hit
CGGCAGGGGGCGTGGGCGTCCGGCCGCAATGGCCAGCGGCTGGCTCAAGC370.1662323658909156No Hit
CGGCAGGGGGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGA370.1662323658909156No Hit
CGGCAGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAG360.16173959924521522No Hit
CGGCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.16173959924521522No Hit
CGGCAGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.15724683259951477No Hit
CGGCAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTT340.15275406595381436No Hit
CGGCAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA320.14376853266241352Illumina Single End Adapter 2 (97% over 34bp)
CGGCAGGGGACTGGAAGAAATGCCCCGGCTCGCCGCCTTCAAAGTAGATG310.1392757660167131No Hit
CGGCAGGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.13029023272531226No Hit
CGGCAGGGGCAGGGGACCTTCTATTTCAACGCGAACATGCTGCTGCGCAC290.13029023272531226No Hit
CGGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1257974660796118No Hit
CGGCAGGGGGCCGGCGGCCTGGTGTGGCGCTCGGCGCAGTCCATCGTGAC280.1257974660796118No Hit
CGGCAGGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT270.12130469943391141No Hit
CGGCAGGGGGCGTGGTGCCGTGGTGGCCAGTGCCAGAGCTCCAGCCACCC260.11681193278821098No Hit
CGGCAGGGGGTGCAGTGCTCGGCAGGGGCAGTGGTATCAACGCGAAAAAA260.11681193278821098No Hit
CGGCAGGGGGCTACATCTGGGGCGAGAAGATCGTGCTCGGCGCGATCTTT260.11681193278821098No Hit
CGGCAGGGGTCAACGCAGAGTCGACCTTCTTTTTTTTTTTTTTTTTTTTT250.11231916614251056No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGT250.11231916614251056No Hit
CGGCAGGGGTCAATGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT240.10782639949681012No Hit
CGGCAGGGGGCGTGGGCGTCCGGCCGCAATGGCCAGCGGCTGGGTCAGGG240.10782639949681012No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG230.10333363285110972Illumina Single End Adapter 2 (100% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTA207.6910114E-444.00000441
AGGGGTT800.044.0000045
CAGCCAG207.6910114E-444.00000415
AGCCTGC207.6910114E-444.00000414
TTTGAGG207.6910114E-444.00000441
CTACAAA207.6910114E-444.00000443
ATCCTAC207.6910114E-444.00000440
GATGGAC207.6910114E-444.00000419
TCCTTTG207.6910114E-444.00000438
TCCTTTA207.6910114E-444.00000438
TTTGACA207.6910114E-444.00000425
TGTTGGT407.699782E-944.00000433
TTCACTA207.6910114E-444.00000419
TAGCGCG207.6910114E-444.00000417
GTAGAGG207.6910114E-444.00000433
ATGGGCA207.6910114E-444.00000430
CTTCAAG207.6910114E-444.00000428
AGGGGAT407.699782E-944.0000045
ACTGTAC207.6910114E-444.00000433
AACAATC207.6910114E-444.00000438
Produced by FastQC (version 0.11.5)