FastQCFastQC Report
Fri 17 Jun 2016
SRR1527215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24355
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG6822.8002463559843975No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGAAG6392.6236912338328886No Hit
CGGCAGGGGGAGCGCACAGAGCGCGGTGACGATGAAGCCCCAATGCAGGCCA5882.414288647095052No Hit
CGGCAGGGGGTTCCGGCATGGCGACCTCGGCGTTTGTCGCGTACCTGTCGAG5142.1104495996715253No Hit
CGGCAGGGGGTTCAATCGCTCGATGGCCGCCTTGTTGGTGGCCAGCGTGGGG4461.8312461506877438No Hit
CGGCAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAAC3541.4535003079449806No Hit
CGGCAGGGGGAAGCCAGATATCGCATCGACTCCCTCGCGAAGCAGGAAGCCA3131.2851570519400535No Hit
CGGCAGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2671.0962841305686717No Hit
CGGCAGGGGGCGTGGGCGTCCGGCCGCAATGGCCAGCGGCTGGCTCAGGCTG2621.075754465202217No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2541.04290700061589No Hit
CGGCAGGGGGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCG2120.870457811537672No Hit
CGGCAGCGGCGCCACCATCACCTCCTGGCCTTCCTTGATCAGCGGATGGCCG2120.870457811537672No Hit
CGGCAGGGGTGGGTGGTCAGGTCCAGGTTTGCGCTGCGCAGGCCCTGGAAGT2120.870457811537672No Hit
CGGCAGGGGGCCGAAGCGCAGCACCACGGCTTACTCCTGCTCGTCCACCACA2100.8622459453910901No Hit
CGGCAGGGGAGAGTGCAGTGCTCGGCAGGGGGCTCAACGGCTACCAGGTGCG2060.8458222130979265No Hit
CGGCAGGGGCGGTGCAATGGCTTTACGAAAGTAATCCGCTACTGGCGGAAAG2030.8335044138780537No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1950.8006569492917266No Hit
CGGCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1940.7965510162184356No Hit
CGGCAGGGGTGGTCCACGACCTGCGGGTGCAGTTGTTCAACAACCTGCTGAC1930.7924450831451447No Hit
CGGCAGGGGTTTCATCGCTGTTAAACTGGGCGCACATTTGATTGATTTTTTC1830.7513857524122357No Hit
CGGCAGGGGGCCATGAAATTAAAAGACGCTTACTCCTTGGAAGGAAAGTTAT1780.7308560870457811No Hit
CGGCAGGGGGAGTGCAGTGCTCGGCAGGGGACCCCATCTTTACCAAAAATAC1770.7267501539724903No Hit
CGGCAGGGTCATTGAAGGTATGCACCTCACTGGCTGCAGACTTCTGCAACCC1770.7267501539724903No Hit
CGGCAGGGGTTCCCTGGTGGTCCAGTAGTTAGGACCTGGCACTTTCACTGCC1740.7144323547526176No Hit
CGGCAGGGGGGGCACGGTGGCTCACACCTGTAAGCCCAGCACTTTGGGAGGC1670.6856908232395812No Hit
CGGCAGGGGCTAGGTGTTTTCTGGATAGCTCGGCGCTGCCCCTCATCCGCCT1580.6487374255799631No Hit
CGGCAGGGGATCTGGGTAGCGCGTTCGGCGCTGTAGAGGATCACGCCGGCGG1570.6446314925066721No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTC1510.6199958940669268No Hit
CGGCAGGGAGGCGGGGATCTGCAGGTGCAAGCAGAAATCATCAAGCAAAGCA1470.603572161773763No Hit
CGGCAGGGGGCATCTAGACTGACTGAAAAAATGCCGTGCGAAAAAACATGAT1370.562512831040854No Hit
CGGCAGGGGCAATGCCCTGCAATAAAGAAGCTCCATCGGCACCTCCTATCCA1310.5378772326011085No Hit
CGGCAGGGGGTGCAGTGCTCGGCAGGGGGACTTTTTTTTTTTTTTTTTTTTT1210.4968179018681996No Hit
CGGCAGGGGACGCGTGGCGATGGACAGGCGGCGTCCATCCTTTGAGGGAAAT1160.476288236501745No Hit
CGGCAGGGGCCCTCCAGCCAGGATGCTTAATGGGCAAACCCAGTTCAGTGGC1140.4680763703551632No Hit
CGGCAGGGGGGTGTGCACCATTGCTCACTATTTGACCACCGCCCAAATGACC1070.43933483884212693No Hit
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.435228905768836No Hit
CGGCAGGGGGGACGGCGTCATGGCCGCCGAAATGGCGCGTTCGGGCTTCATC1030.42291110654896324No Hit
CGGCAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.4146992404023815No Hit
CGGCAGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGC1000.41059330732909055No Hit
CGGCAGGGGCAACGTGAGTGCCTGTTTTTGAGTCGTTGAGTTCTACAGTCCA990.4064873742557996No Hit
CGGCAGGGGAGTGGTATCAAGTGCAGTGCTCGGCAGGGGTAAAAAAAAAAAA970.3982755081092178No Hit
CGGCAGGGGTTCCCGGGCGCTTTCGCGCTGACGCGCGAAGAAGCGGCTTCGC970.3982755081092178No Hit
CGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGC950.390063641962636No Hit
CGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAGAAGTCCTTTACCAGCTTT940.3859577088893451No Hit
CGGCAGGGGGGCTGGCTCGGGGCAGCCGGGCGCGAGCGGCGTAGACAAGGGG860.35311024430301785No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGGAG820.3366865120098542No Hit
CGGCAGAAGCCTGAATGAGCTTAATAGAGGCCAAAGCGGTCTGGAAACGTAC820.3366865120098542No Hit
CGGCAGGGGGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.33258057893656334No Hit
CGGCAGGGGGAGTGCAGTGCTTTGCAGGGGTGCACCTATGCGCTGACCATCA810.33258057893656334No Hit
CGGCAGGGGGGGAATACCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG800.3284746458632724No Hit
CGGCAGGGGGCGTTTCTCCTTCCTGCCACCTTGTGAAGGTGGGTGCCTTGCT800.3284746458632724No Hit
CGGCAGGGGAGCCTTCTCGCACCACTCCGCCGCCGCGCTGGCCGCTGCCTAC780.32026277971669065No Hit
CGGCAGGGGGATGCAGAAAAGGTCTTTGACAAAATTCAACAATCCTTCATGC730.2997331143502361No Hit
CGGCAGGGGAGAGTAATTTATTTGCAGTCAGTTGATTATGTACAGAGTTTTA730.2997331143502361No Hit
CGGCAGGGGGACTTATCGATAAGATTTCCGCCACTGTACTTTTTGTAAGCGT720.2956271812769452No Hit
CGGCAGGGGGTGCTCGGCAGGGGCTGGTGATACGGAAGATGTCCATCCAGCC720.2956271812769452No Hit
CGGCAGGGGAGAACGGCGAGCAGGCCGGGAATGAGCGTCAGCCAGAAGACCA710.2915212482036543No Hit
CGGCAGGGGGGTCCGAGGGGCCGTGGGCCGGGGCGTGTGAGGAGACGCGGGG710.2915212482036543No Hit
CGGCAGCAATAAACTCAACAGGAGCAGGAAAGCGAGGGTATCCTACAAAGTC690.28330938205707246No Hit
CGGCAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGCAGCGCCGG660.27099158283719976No Hit
CGGCAGGGGTTGCCGGATGGCCTGTTCAACGACGAAACCGACGGGCATGTGG650.2668856497639089No Hit
CGGCAGGGGAGAGTAACTTTCACTATACACCTATCGGAATTTTTTAAATAAA640.26277971669061795No Hit
CGGCAGGGGTTTAGTCCTTTTTATTCACTTCAGTCTCTTCAGCTCGGTTCTT630.258673783617327No Hit
CGGCAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.25456785054403613No Hit
CGGCAGGGGGTGCTCGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.25456785054403613No Hit
CGGCAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.24635598439745432No Hit
CGGCAGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.24635598439745432No Hit
CGGCAGGGGGCCACAAGCCTGGTGCCCAAGGAAAAAGCCGCCAGCGCGATTG600.24635598439745432No Hit
CGGCAGATTGCGATAAACGGTCACATTAAATTTAACCTGACTATTCCACTGC600.24635598439745432No Hit
CGGCAGGGGGTTCCGACATGGCGACCTCGGCGTTTGTCGCGTACCTGTCGAG560.22993225210429072No Hit
CGGCAGGGGCTGGCCAACTTTGAAAAGGCCACCGGCATCAAGCCGGTCTACG500.20529665366454528No Hit
CGGCAGGGGGTTCAAACGCTCGATGGCCGCCTTGTTGGTGGCCAGCGTGGGG480.19708478751796343No Hit
CGGCAGCAAGAACCATACGACCAATATCACGAAAATAGTCACGCAAAGCATT480.19708478751796343No Hit
CGGCAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.19297885444467255No Hit
CGGCAGGGGGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGA460.18887292137138165No Hit
CGGCAGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.18476698829809074No Hit
CGGCAGGGGCAGGGGACCTTCTATTTCAACGCGAACATGCTGCTGCGCACCC430.17655512215150893No Hit
CGGCAGGGGGGGAATACCTTCGGCAACATTAACTGGTTGATGCTGAAAAAAC420.17244918907821802No Hit
CGGCAGGGGAGGCAGTGGCAAGTCCCGGATGTTGGCCGCCAGTGGCTTGCAG400.1642373229316362No Hit
CGGCAGGGGGTGCTCGGCAGGGGCTGGGGATACGGAAGATGTCCATCCAGCC390.16013138985834532No Hit
CGGCAGGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGAAG390.16013138985834532No Hit
CGGCAGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG380.1560254567850544No Hit
CGGCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.1560254567850544No Hit
CGGCAGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.1519195237117635No Hit
CGGCAGGGGACGCAGAGTCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.1519195237117635No Hit
CGGCAGGGGGCCGGCGGCCTGGTGTGGCGCTCGGCGCAGTCCATCGTGACGC370.1519195237117635No Hit
CGGCAGGGGAAGCACTGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGAAG350.14370765756518167No Hit
CGGCAGGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT340.1396017244918908No Hit
CGGCAGGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.13549579141859988No Hit
CGGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.13138985834530897No Hit
CGGCAGCGGCGCCACAATCACCTCCTGGCCTTCCTTGATCAGCGGATGGCCG320.13138985834530897No Hit
CGGCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.13138985834530897No Hit
CGGCAGGGGGTGCAGTGCTCGGCAGGGGCAGTGGTATCAACGCGAAAAAAAA300.12317799219872716No Hit
CGGCAGGGGGCTACATCTGGGGCGAGAAGATCGTGCTCGGCGCGATCTTTTC300.12317799219872716No Hit
CGGCAGGGGACTGGAAGAAATGCCCCGGCTCGCCGCCTTCAAAGTAGATGTG290.11907205912543625No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGTAG290.11907205912543625No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC290.11907205912543625Illumina Single End Adapter 2 (100% over 25bp)
CGGCAGGGGGCGTGGACGTCCGGCCGCAATGGCCAGCGGCTGGCTCAGGCTG290.11907205912543625No Hit
CGGCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGCAGCGCCGGA290.11907205912543625No Hit
CGGCAGGGGGTGGTAACAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.11496612605214536No Hit
CGGCAGGGGAAGCAGGGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGAAG270.11086019297885444No Hit
CGGCAGGGGGGGAATGCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG270.11086019297885444No Hit
CGGCAGGGGATTCCAAGTACTTTTTTTTTTTTTTAATTTTAAGCTCTTAACT250.10264832683227264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCAA550.046.037
CGTTTGT550.046.031
TGATGCT550.046.038
AGCCCCA550.046.036
TTTGAGG301.7869679E-646.041
CGCTGCG301.7869679E-646.032
GAGGCGG301.7869679E-646.08
GATGGAC301.7869679E-646.019
GAGGTGG301.7869679E-646.031
TCCTTTG405.2314135E-946.038
TCCTTTA206.1899394E-446.038
TGTTGGT850.046.033
TTCACTG253.3189754E-546.044
GTTGATG550.046.036
GGAGAGT550.046.08
GAGGTAT253.3189754E-546.015
TTGTCGC550.046.034
GTTTGTC550.046.032
AGGGGCG206.1899394E-446.05
TGGTTGA550.046.034
Produced by FastQC (version 0.11.5)