FastQCFastQC Report
Fri 17 Jun 2016
SRR1527214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23136
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG6502.809474412171508No Hit
CGGCAGGGGGAGCGCACAGAGCGCGGTGACGATGAAGCCCCAATGCAGGCCA5832.5198824343015214No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGAAG5432.3469917012448134No Hit
CGGCAGGGGGTTCCGGCATGGCGACCTCGGCGTTTGTCGCGTACCTGTCGAG4321.8672199170124482No Hit
CGGCAGGGGGTTCAATCGCTCGATGGCCGCCTTGTTGGTGGCCAGCGTGGGG3741.6165283540802216No Hit
CGGCAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAAC3411.473893499308437No Hit
CGGCAGGGGGAAGCCAGATATCGCATCGACTCCCTCGCGAAGCAGGAAGCCA2791.2059128630705396No Hit
CGGCAGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2701.1670124481327802No Hit
CGGCAGGGGAGAGTGCAGTGCTCGGCAGGGGGCTCAACGGCTACCAGGTGCG2331.007088520055325No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2280.9854771784232365No Hit
CGGCAGGGGGCGTGGGCGTCCGGCCGCAATGGCCAGCGGCTGGCTCAGGCTG2240.9681881051175657No Hit
CGGCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA2110.9119986168741356No Hit
CGGCAGCGGCGCCACCATCACCTCCTGGCCTTCCTTGATCAGCGGATGGCCG2060.890387275242047No Hit
CGGCAGGGTCATTGAAGGTATGCACCTCACTGGCTGCAGACTTCTGCAACCC2040.8817427385892116No Hit
CGGCAGGGGATCTGGGTAGCGCGTTCGGCGCTGTAGAGGATCACGCCGGCGG1840.7952973720608575No Hit
CGGCAGGGGTTTCATCGCTGTTAAACTGGGCGCACATTTGATTGATTTTTTC1820.7866528354080221No Hit
CGGCAGGGGCGGTGCAATGGCTTTACGAAAGTAATCCGCTACTGGCGGAAAG1810.7823305670816044No Hit
CGGCAGGGGTGGGTGGTCAGGTCCAGGTTTGCGCTGCGCAGGCCCTGGAAGT1810.7823305670816044No Hit
CGGCAGGGGTGGTCCACGACCTGCGGGTGCAGTTGTTCAACAACCTGCTGAC1800.7780082987551867No Hit
CGGCAGGGGTTCCCTGGTGGTCCAGTAGTTAGGACCTGGCACTTTCACTGCC1750.7563969571230982No Hit
CGGCAGGGGGCCGAAGCGCAGCACCACGGCTTACTCCTGCTCGTCCACCACA1730.7477524204702628No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.7261410788381742No Hit
CGGCAGGGGCTAGGTGTTTTCTGGATAGCTCGGCGCTGCCCCTCATCCGCCT1650.7131742738589212No Hit
CGGCAGGGGGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCG1570.6785961272475796No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTC1470.6353734439834025No Hit
CGGCAGGGGGGGCACGGTGGCTCACACCTGTAAGCCCAGCACTTTGGGAGGC1440.6224066390041494No Hit
CGGCAGGGGGCCATGAAATTAAAAGACGCTTACTCCTTGGAAGGAAAGTTAT1350.5835062240663901No Hit
CGGCAGGGAGGCGGGGATCTGCAGGTGCAAGCAGAAATCATCAAGCAAAGCA1310.5662171507607192No Hit
CGGCAGGGGCAATGCCCTGCAATAAAGAAGCTCCATCGGCACCTCCTATCCA1280.5532503457814661No Hit
CGGCAGGGGGTGCAGTGCTCGGCAGGGGGACTTTTTTTTTTTTTTTTTTTTT1170.5057053941908713No Hit
CGGCAGGGGTTCCCGGGCGCTTTCGCGCTGACGCGCGAAGAAGCGGCTTCGC1120.48409405255878285No Hit
CGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGC1090.47112724757952973No Hit
CGGCAGGGGGAGTGCAGTGCTCGGCAGGGGACCCCATCTTTACCAAAAATAC1070.46248271092669435No Hit
CGGCAGGGGAGAGTAACTTTCACTATACACCTATCGGAATTTTTTAAATAAA1050.453838174273859No Hit
CGGCAGGGGACGCGTGGCGATGGACAGGCGGCGTCCATCCTTTGAGGGAAAT1050.453838174273859No Hit
CGGCAGGGGGGTGTGCACCATTGCTCACTATTTGACCACCGCCCAAATGACC1050.453838174273859No Hit
CGGCAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.4365491009681881No Hit
CGGCAGGGGCAACGTGAGTGCCTGTTTTTGAGTCGTTGAGTTCTACAGTCCA1000.4322268326417704No Hit
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.4149377593360996No Hit
CGGCAGGGGGCATCTAGACTGACTGAAAAAATGCCGTGCGAAAAAACATGAT960.4149377593360996No Hit
CGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAGAAGTCCTTTACCAGCTTT920.39764868603042874No Hit
CGGCAGGGGGCGTTTCTCCTTCCTGCCACCTTGTGAAGGTGGGTGCCTTGCT920.39764868603042874No Hit
CGGCAGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGC830.35874827109266944No Hit
CGGCAGGGGGGACGGCGTCATGGCCGCCGAAATGGCGCGTTCGGGCTTCATC790.3414591977869986No Hit
CGGCAGGGGGGCTGGCTCGGGGCAGCCGGGCGCGAGCGGCGTAGACAAGGGG750.3241701244813278No Hit
CGGCAGGGGGACTTATCGATAAGATTTCCGCCACTGTACTTTTTGTAAGCGT750.3241701244813278No Hit
CGGCAGGGGCCCTCCAGCCAGGATGCTTAATGGGCAAACCCAGTTCAGTGGC750.3241701244813278No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGGAG740.31984785615491007No Hit
CGGCAGAAGCCTGAATGAGCTTAATAGAGGCCAAAGCGGTCTGGAAACGTAC740.31984785615491007No Hit
CGGCAGCAATAAACTCAACAGGAGCAGGAAAGCGAGGGTATCCTACAAAGTC720.3112033195020747No Hit
CGGCAGGGGGTGCTCGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.306881051175657No Hit
CGGCAGGGGAGTGGTATCAAGTGCAGTGCTCGGCAGGGGTAAAAAAAAAAAA670.2895919778699862No Hit
CGGCAGGGGAGCCTTCTCGCACCACTCCGCCGCCGCGCTGGCCGCTGCCTAC630.27230290456431533No Hit
CGGCAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGCAGCGCCGG620.26798063623789764No Hit
CGGCAGATTGCGATAAACGGTCACATTAAATTTAACCTGACTATTCCACTGC620.26798063623789764No Hit
CGGCAGGGGGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.26365836791147995No Hit
CGGCAGGGGGAGTGCAGTGCTTTGCAGGGGTGCACCTATGCGCTGACCATCA610.26365836791147995No Hit
CGGCAGGGGGTGCTCGGCAGGGGCTGGTGATACGGAAGATGTCCATCCAGCC610.26365836791147995No Hit
CGGCAGGGGAGAGTAATTTATTTGCAGTCAGTTGATTATGTACAGAGTTTTA600.2593360995850622No Hit
CGGCAGGGGGCCACAAGCCTGGTGCCCAAGGAAAAAGCCGCCAGCGCGATTG600.2593360995850622No Hit
CGGCAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.25069156293222683No Hit
CGGCAGGGGAGGCAGTGGCAAGTCCCGGATGTTGGCCGCCAGTGGCTTGCAG560.24204702627939143No Hit
CGGCAGGGGTTTAGTCCTTTTTATTCACTTCAGTCTCTTCAGCTCGGTTCTT550.2377247579529737No Hit
CGGCAGGGGGATGCAGAAAAGGTCTTTGACAAAATTCAACAATCCTTCATGC540.23340248962655602No Hit
CGGCAGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.23340248962655602No Hit
CGGCAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.22908022130013833No Hit
CGGCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.22908022130013833No Hit
CGGCAGGGGTTGCCGGATGGCCTGTTCAACGACGAAACCGACGGGCATGTGG500.2161134163208852No Hit
CGGCAGCAAGAACCATACGACCAATATCACGAAAATAGTCACGCAAAGCATT490.2117911479944675No Hit
CGGCAGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.20314661134163212No Hit
CGGCAGGGGGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGA470.20314661134163212No Hit
CGGCAGGGGGGTCCGAGGGGCCGTGGGCCGGGGCGTGTGAGGAGACGCGGGG460.19882434301521437No Hit
CGGCAGGGGAGAACGGCGAGCAGGCCGGGAATGAGCGTCAGCCAGAAGACCA450.19450207468879668No Hit
CGGCAGGGGGGGAATTCCTTGGATTCTGTTTTGCTACCCATTGTAGCGCCGG410.17721300138312587No Hit
CGGCAGGGGAAGCACTGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGAAG400.17289073305670816No Hit
CGGCAGGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT390.16856846473029047No Hit
CGGCAGGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.15992392807745504No Hit
CGGCAGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG360.15560165975103735No Hit
CGGCAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA340.14695712309820194No Hit
CGGCAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.14695712309820194No Hit
CGGCAGGGGACGCAGAGTCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.14263485477178423No Hit
CGGCAGGGGGCTACATCTGGGGCGAGAAGATCGTGCTCGGCGCGATCTTTTC320.13831258644536654No Hit
CGGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.13399031811894882No Hit
CGGCAGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.13399031811894882No Hit
CGGCAGGGGGGGAATTCCTTGGATTCTGTTTTGCTACCCGTTGTAGCGCCGG310.13399031811894882No Hit
CGGCAGGGGCTGGCCAACTTTGAAAAGGCCACCGGCATCAAGCCGGTCTACG300.1296680497925311No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGGCAGGGGTAG290.12534578146611342No Hit
CGGCAGGGGGTGCTCGGCAGGGGCTGGGGATACGGAAGATGTCCATCCAGCC280.12102351313969571No Hit
CGGCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGCAGCGCCGGA280.12102351313969571No Hit
CGGCAGGGGGAGCGCACAGAGCGCGGTGACGATGAAGCCACAATGCAGGCCA280.12102351313969571No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC270.11670124481327801Illumina Single End Adapter 2 (100% over 25bp)
CGGCAGGGGTCAACGCAGAGTCGACCTTCTTTTTTTTTTTTTTTTTTTTTTT270.11670124481327801No Hit
CGGCAGGGGGTTCAATCGCTGGATGGCCGCCTTGTTGGTAGCCAGCGTGGGG260.1123789764868603No Hit
CGGCAGGGGGTTCCGGCATGGCGACCTCGGCGTTTGTCGAGTACCTGTCGAG260.1123789764868603No Hit
CGGCAGGGGAAGCAGTGGTAGCAACGCAGAGTGCAGTGCACGGCAGGGGAAG250.1080567081604426No Hit
CGGCAGGGGTGCTCGGCAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTC250.1080567081604426No Hit
CGGCAGGGGACTGGAAGAAATGCCCCGGCTCGCCGCCTTCAAAGTAGATGTG240.1037344398340249No Hit
CGGCAGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG240.1037344398340249No Hit
CGGCAGGGGAGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTT240.1037344398340249No Hit
CGGCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.1037344398340249No Hit
CGGCAGGGGCAGGGGACCTTCTATTTCAACGCGAACATGCTGCTGCGCACCC240.1037344398340249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGT3100.046.0000045
GCCCCAA501.4551915E-1146.037
CGTTTGT550.046.031
AGGGGTT550.046.05
TGATGCT301.7829207E-646.038
AGCCCCA501.4551915E-1146.036
TTTGAGG253.31361E-546.041
GATGGAC253.31361E-546.019
TGTTGGT452.8194336E-1046.033
GTTGATG301.7829207E-646.036
TAGCGCG301.7829207E-646.017
GTAGAGG452.8194336E-1046.033
TTGTCGC452.8194336E-1046.034
GTTTGTC550.046.032
TTGTTGG452.8194336E-1046.032
AGGGGCG253.31361E-546.05
TTGTTCA206.183268E-446.033
TCCTTGA206.183268E-446.032
GTATGCA452.8194336E-1046.018
GTGTTTT253.31361E-546.014
Produced by FastQC (version 0.11.5)