FastQCFastQC Report
Fri 17 Jun 2016
SRR1527213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909614
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC188420.4819401608445233No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC160000.40924756254709543No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG99010.2532475072986745No Hit
ACTTATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG93880.24012600732450823No Hit
ACTTATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA84140.2152130619544538No Hit
ACTTATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA83970.2147782364192475No Hit
ACTTATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT75490.19308811560425146No Hit
ACTTATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG74070.189456043486646No Hit
ACTTATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA71080.18180822966154714No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG70130.17937832225892378No Hit
ACTTATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC64980.16620566633943915No Hit
ACTTATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT62380.15955539344804884No Hit
ACTTATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG60570.15492578039673482No Hit
ACTTATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC58630.1499636537008513No Hit
ACTTATGGGTGCTGTTTGACACCTCTTTTTAATCTGGTTTTAATTTGCTT54540.13950226288324116No Hit
ACTTATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC54240.13873492370346535No Hit
ACTTATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT53430.13666310791807068No Hit
ACTTATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC51910.13277525607387328No Hit
ACTTATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC50100.12814564302255926No Hit
ACTTATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG49970.12781312937798975No Hit
ACTTATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA48580.12425779117836186No Hit
ACTTATGGGGAACTTTACAGTTTAAAAGATTAAAAATCATTTCTTAAGTC48520.1241043233424067No Hit
ACTTATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC47470.12141863621319138No Hit
ACTTATGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT44350.11343830874352301No Hit
ACTTATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC44240.1131569510442719No Hit
ACTTATGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCT43540.11136649295812835No Hit
ACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT43430.11108513525887721No Hit
ACTTATGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC42990.1099597044618727No Hit
ACTTATGGGATTTTGTTTTGTCTCGTGATGGTGGTGGTGGTAGGGGTACG42700.10921794325475609No Hit
ACTTATGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATAC42310.10822040232104754No Hit
ACTTATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA41050.10499757776598917No Hit
ACTTATGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC40770.10428139453153176No Hit
ACTTATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC40710.10412792669557659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA454.802132E-1044.038
CCGTACG351.4472789E-744.038
GCGTAAT254.4448083E-544.029
TAATCGA302.529283E-644.029
TATGGGA1307050.042.183854
ACTTATG4075250.042.127281
CTTATGG4055150.042.122882
TTATGGG3974400.042.115743
TATGGGT231150.042.0869564
TATGGGC326000.041.9957054
ATGGGAT431700.041.9768335
TATGGGG2119550.041.9095574
ATGGGAC244250.041.901335
ATGGGAG432100.041.764875
TTAATCG950.041.6842120
ATGGGGT378300.041.6621745
ATGGGGC460900.041.6420065
ATGGGGA591450.041.578495
ATGGGGG704650.041.5585065
TGGGATC128200.041.1684886
Produced by FastQC (version 0.11.5)