FastQCFastQC Report
Fri 17 Jun 2016
SRR1527212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4158412
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA164510.3956077464185848No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC147190.3539572317509665No Hit
ACTTATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG89440.21508210345680032No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT87120.20950305068377062No Hit
ACTTATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC82010.19721470599834745No Hit
ACTTATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA80700.1940644649928867No Hit
ACTTATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG72960.175451590655279No Hit
ACTTATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG70550.16965610911088175No Hit
ACTTATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC67150.16147991108144166No Hit
ACTTATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC61650.1482537083867592No Hit
ACTTATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC56350.13550845851733787No Hit
ACTTATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA54750.1316608359152484No Hit
ACTTATGGGTGCTGTTTGACACCTCTTTTTAATCTGGTTTTAATTTGCTTTG52660.12663487889126906No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT49570.11920415774098383No Hit
ACTTATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT48610.11689558417973016No Hit
ACTTATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT47690.1146832011835287No Hit
ACTTATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG47090.11324034270774516No Hit
ACTTATGGGGAACTTTACAGTTTAAAAGATTAAAAATCATTTCTTAAGTCCT46510.11184557951448774No Hit
ACTTATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT46060.1107634356576501No Hit
ACTTATGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATACTA45790.11011414934354749No Hit
ACTTATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG45620.10970533944207549No Hit
ACTTATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG45490.10939272010565572No Hit
ACTTATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG45210.10871938615029007No Hit
ACTTATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT43160.10378961969136294No Hit
ACTTATGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT42920.10321247630104954No Hit
ACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC42350.10184176074905518No Hit
ACTTATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC41970.10092795038105892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGGA1394000.044.0035864
CGTTAAT1150.044.021
CTTATGG4315450.043.9939042
ACTTATG4339200.043.9932251
TTATGGG4234300.043.9521983
TATGGGT244600.043.88434
TATGGGG2263950.043.769034
ATGGGAG455050.043.6800355
ATGGGAC267550.043.678945
ATGGGAT461250.043.5516555
ATGGGGT397650.043.5475965
TATGGGC343300.043.5211224
ATGGGGA637000.043.4111485
ATGGGGG766250.043.370575
ATGGGGC481850.043.3508345
TGGGATA57500.042.26
ATGGGAA229900.042.1483275
TGGGGGG303600.042.0833326
TGGGATT179200.041.661836
TGGGGAG290200.041.601316
Produced by FastQC (version 0.11.5)