##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527212_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4158412
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.442144501314445	33.0	31.0	34.0	31.0	34.0
2	32.93671021534182	34.0	31.0	34.0	31.0	34.0
3	33.029721201266256	34.0	31.0	34.0	31.0	34.0
4	36.54352647116255	37.0	37.0	37.0	35.0	37.0
5	36.03076607127913	37.0	35.0	37.0	35.0	37.0
6	36.354218389135085	37.0	37.0	37.0	35.0	37.0
7	36.57667037320977	37.0	37.0	37.0	35.0	37.0
8	36.659524116417515	37.0	37.0	37.0	35.0	37.0
9	38.738816884907024	39.0	39.0	39.0	39.0	39.0
10	38.21898022610554	39.0	39.0	39.0	37.0	39.0
11	37.95918249562573	39.0	38.0	39.0	35.0	39.0
12	37.60665585805351	39.0	37.0	39.0	35.0	39.0
13	37.5026769834254	39.0	37.0	39.0	35.0	39.0
14	38.32470688330064	40.0	38.0	40.0	35.0	40.0
15	38.23809978424456	40.0	38.0	40.0	35.0	40.0
16	33.14941905708237	40.0	35.0	40.0	3.0	40.0
17	34.89871277785847	40.0	35.0	40.0	17.0	40.0
18	37.00560189803223	40.0	35.0	40.0	32.0	40.0
19	37.876530271651774	40.0	37.0	40.0	34.0	40.0
20	38.102002398992695	40.0	38.0	40.0	34.0	40.0
21	38.292267336666015	40.0	38.0	40.0	35.0	40.0
22	38.32822433178819	40.0	38.0	40.0	35.0	40.0
23	38.30277110589331	40.0	38.0	40.0	35.0	40.0
24	38.258708853283416	40.0	38.0	40.0	35.0	40.0
25	38.2174505556448	40.0	38.0	40.0	35.0	40.0
26	38.12990632000869	40.0	38.0	40.0	34.0	40.0
27	38.022589392296865	40.0	38.0	40.0	34.0	40.0
28	37.9305107334242	40.0	38.0	40.0	34.0	40.0
29	37.723613725624105	40.0	37.0	40.0	34.0	40.0
30	37.72038052025629	40.0	37.0	40.0	34.0	40.0
31	37.70632804060781	40.0	37.0	40.0	34.0	40.0
32	37.63582179928299	40.0	37.0	40.0	33.0	40.0
33	37.3206221509557	40.0	37.0	40.0	33.0	40.0
34	37.26662245107026	40.0	36.0	40.0	33.0	40.0
35	37.21539111564703	40.0	36.0	40.0	33.0	40.0
36	37.19137690060533	39.0	36.0	40.0	33.0	40.0
37	36.889099011834325	39.0	35.0	40.0	32.0	40.0
38	36.85195815133277	39.0	35.0	40.0	31.0	40.0
39	36.474856748201	39.0	35.0	40.0	31.0	40.0
40	36.43659983666842	39.0	35.0	40.0	31.0	40.0
41	36.384806026916046	39.0	35.0	40.0	31.0	40.0
42	36.351845127418834	39.0	35.0	40.0	31.0	40.0
43	36.257135416115574	38.0	35.0	40.0	31.0	40.0
44	36.53004464204124	39.0	35.0	40.0	31.0	40.0
45	36.602879175993145	39.0	35.0	40.0	32.0	40.0
46	36.53384729555417	39.0	35.0	40.0	32.0	40.0
47	36.21313761118427	39.0	35.0	40.0	31.0	40.0
48	36.18671069629465	38.0	35.0	40.0	31.0	40.0
49	36.0991676630406	38.0	35.0	40.0	31.0	40.0
50	35.59612611737365	38.0	35.0	40.0	30.0	40.0
51	35.67228066867833	37.0	35.0	40.0	30.0	40.0
52	35.357901525870936	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	11.0
15	11.0
16	71.0
17	130.0
18	309.0
19	666.0
20	1236.0
21	2144.0
22	3668.0
23	5876.0
24	8760.0
25	12672.0
26	17936.0
27	24458.0
28	32733.0
29	44170.0
30	58404.0
31	77718.0
32	105039.0
33	156000.0
34	224366.0
35	303681.0
36	432654.0
37	807966.0
38	1141923.0
39	695807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.7932383804202	0.17222920672602907	0.0217390677018054	0.012793345151947426
8	99.44796234716522	0.44824803314342115	0.05326552539767584	0.05052409429368711
9	97.9653771680151	1.0485493019931647	0.33207387820158274	0.6539996517901545
10	52.93015699262122	32.71616665207777	6.0723901335413615	8.281286221759652
11	32.75798550023422	23.518857679325667	23.741827409116752	19.98132941132336
12	28.763359667103693	17.096766746536897	27.64461530026366	26.49525828609575
13	23.895612074994013	16.820411253141824	31.54259366315795	27.74138300870621
14	25.351865086961077	18.610132906503733	31.09872230072441	24.939279705810776
15	25.235474503247872	19.21661441915808	29.351781401169486	26.196129676424558
16	27.757783500047616	27.34096573403501	23.37187849592585	21.529372269991526
17	29.188666250482154	19.005620414716002	25.34715174927352	26.458561585528322
18	28.13044979670124	19.22060632760775	25.215130198739327	27.433813676951686
19	27.817854507922736	20.89028215578447	24.37793561580719	26.91392772048561
20	26.750499950461858	21.196456724345737	25.926723951354507	26.126319373837898
21	28.098538576745163	19.386631242887912	27.252638747675796	25.262191432691132
22	28.101400246055462	18.512836149953397	26.70033657078712	26.68542703320402
23	26.85051409047492	20.13453693381031	26.65240962174984	26.362539353964927
24	27.419769854454056	20.379822874693513	25.04653218584402	27.153875085008412
25	26.822883350663666	21.05633111870589	25.4715021022448	26.649283428385644
26	27.017044006221607	20.7834384856527	24.468907842705338	27.73060966542036
27	27.440354635375236	18.85582765728841	25.034893127472696	28.66892457986366
28	28.122634313290746	20.772136094259057	23.39809042490258	27.707139167547613
29	28.665197195467883	20.448454842858283	24.72037402739315	26.165973934280686
30	28.470459396519633	20.149759090729827	24.160184224170187	27.219597288580356
31	29.804911105489307	20.97315032757697	23.542280081915884	25.679658485017836
32	30.955879311621842	20.255376331157184	23.955683082869133	24.833061274351845
33	30.843769208053455	20.646727645072204	24.36153512446578	24.147968022408552
34	28.036183042950046	21.88991855544857	25.99655830158243	24.077340100018947
35	27.822712131457873	23.756111708027007	25.800233358310816	22.620942802204304
36	29.36091950484945	23.116011592886903	23.36271634460462	24.160352557659028
37	29.262252032747117	23.373201116195315	23.319647019102486	24.04489983195508
38	29.682003610993814	22.85810064034059	23.007099825606506	24.45279592305909
39	29.183159340632912	22.00955557073229	22.48266886494171	26.32461622369308
40	27.59226358523398	22.178658584094123	23.94904593388053	26.28003189679137
41	26.312351926648926	21.76297105722088	24.601386298423535	27.323290717706662
42	25.908447743994582	20.53271777784404	25.0624276767189	28.496406801442475
43	25.846669353589785	20.217910106069336	26.692376801529043	27.243043738811835
44	26.073414563059167	20.466153906827895	26.721594685663664	26.738836844449278
45	27.18655582948491	20.18029959513391	25.346310081829316	27.286834493551865
46	26.296191911720147	21.66831954120948	25.19675780081435	26.838730746256022
47	26.45836920439822	22.386093537629268	25.363961050516398	25.791576207456114
48	27.184632018183862	22.120848054497728	25.636108206690437	25.058411720627973
49	27.000451133750097	22.308155132295692	24.41133778952158	26.280055944432632
50	25.465442096646505	22.775761516655876	24.66566564351969	27.093130743177923
51	25.39801251054489	23.530063880154252	24.938197561953938	26.133726047346922
52	26.95916133370142	22.92961832545693	24.391546580762082	25.71967376007957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.0
2	4.0
3	3.5
4	3.0
5	20.5
6	38.0
7	602.5
8	1167.0
9	2922.5
10	4678.0
11	6147.0
12	7616.0
13	7176.5
14	7283.5
15	7830.0
16	8256.0
17	8682.0
18	9219.5
19	9757.0
20	11922.5
21	14088.0
22	16225.5
23	18363.0
24	20964.5
25	23566.0
26	27984.5
27	32403.0
28	34836.0
29	37269.0
30	39809.0
31	42349.0
32	52998.5
33	63648.0
34	69420.5
35	75193.0
36	78248.0
37	81303.0
38	88260.5
39	101597.5
40	107977.0
41	118748.0
42	129519.0
43	147810.5
44	166102.0
45	175010.5
46	183919.0
47	202342.5
48	220766.0
49	236272.5
50	251779.0
51	269237.0
52	286695.0
53	316121.0
54	345547.0
55	366926.5
56	388306.0
57	375479.0
58	362652.0
59	357021.0
60	351390.0
61	303360.0
62	255330.0
63	220293.5
64	161665.0
65	138073.0
66	116351.5
67	94630.0
68	82359.0
69	70088.0
70	59390.5
71	48693.0
72	37076.5
73	25460.0
74	17748.5
75	10037.0
76	6544.0
77	3051.0
78	2690.0
79	2329.0
80	1445.0
81	561.0
82	367.5
83	174.0
84	105.5
85	37.0
86	43.5
87	50.0
88	43.5
89	39.0
90	41.0
91	20.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4158412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.832833927786983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.7005511524676	5.302871739969263
2	10.291861330431137	1.612288812181598
3	4.547600265021534	1.0686179293762124
4	2.4519952775770686	0.768242872039165
5	1.3863725571288616	0.5429613001015872
6	0.8859265453869414	0.4163589301340369
7	0.5964121957815425	0.3270118377444519
8	0.42093952538682367	0.263771951679717
9	0.3101867795330141	0.21866773776094564
>10	4.749834770539677	10.315156415363282
>50	3.554710350938009	20.186918740792084
>100	2.9104341211240565	40.1724508569754
>500	0.12415860020376251	6.480981745726069
>1k	0.06439170956126365	9.294583691155198
>5k	0.003913308315877902	2.160540885776984
>10k+	7.115106028868912E-4	0.8685745532239867
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	16451	0.3956077464185848	No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14719	0.3539572317509665	No Hit
ACTTATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	8944	0.21508210345680032	No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8712	0.20950305068377062	No Hit
ACTTATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	8201	0.19721470599834745	No Hit
ACTTATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	8070	0.1940644649928867	No Hit
ACTTATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7296	0.175451590655279	No Hit
ACTTATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	7055	0.16965610911088175	No Hit
ACTTATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	6715	0.16147991108144166	No Hit
ACTTATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6165	0.1482537083867592	No Hit
ACTTATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	5635	0.13550845851733787	No Hit
ACTTATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	5475	0.1316608359152484	No Hit
ACTTATGGGTGCTGTTTGACACCTCTTTTTAATCTGGTTTTAATTTGCTTTG	5266	0.12663487889126906	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	4957	0.11920415774098383	No Hit
ACTTATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	4861	0.11689558417973016	No Hit
ACTTATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	4769	0.1146832011835287	No Hit
ACTTATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	4709	0.11324034270774516	No Hit
ACTTATGGGGAACTTTACAGTTTAAAAGATTAAAAATCATTTCTTAAGTCCT	4651	0.11184557951448774	No Hit
ACTTATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4606	0.1107634356576501	No Hit
ACTTATGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATACTA	4579	0.11011414934354749	No Hit
ACTTATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	4562	0.10970533944207549	No Hit
ACTTATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	4549	0.10939272010565572	No Hit
ACTTATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	4521	0.10871938615029007	No Hit
ACTTATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	4316	0.10378961969136294	No Hit
ACTTATGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	4292	0.10321247630104954	No Hit
ACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4235	0.10184176074905518	No Hit
ACTTATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4197	0.10092795038105892	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.214292378917721E-5	0.0	0.0	0.0	0.0
8	2.645240538936498E-4	0.0	0.0	0.0	0.0
9	7.695245204178903E-4	0.0	0.0	0.0	0.0
10	0.006204291445869241	0.0	0.0	0.0	0.0
11	0.010003818765432574	0.0	0.0	0.0	0.0
12	0.01370715551994367	0.0	0.0	0.0	0.0
13	0.018300255001187954	0.0	0.0	0.0	0.0
14	0.027294072833572047	0.0	0.0	0.0	0.0
15	0.033113602019232344	0.0	0.0	0.0	0.0
16	0.043357897197295506	0.0	0.0	0.0	0.0
17	0.049369807513060276	0.0	0.0	0.0	0.0
18	0.05504505085114222	0.0	0.0	0.0	0.0
19	0.0649286314102595	0.0	0.0	0.0	0.0
20	0.07219101907170333	0.0	0.0	0.0	0.0
21	0.0783231675937834	0.0	0.0	0.0	0.0
22	0.08498436422365076	0.0	0.0	0.0	0.0
23	0.0904191311491021	0.0	0.0	0.0	0.0
24	0.09777770937559818	0.0	0.0	0.0	0.0
25	0.10289985696462976	0.0	0.0	0.0	0.0
26	0.107877718706083	0.0	0.0	0.0	0.0
27	0.11388962902184777	0.0	0.0	0.0	0.0
28	0.12247463695275985	0.0	0.0	0.0	0.0
29	0.12865488075736603	0.0	0.0	0.0	0.0
30	0.13447440994302634	0.0	0.0	0.0	0.0
31	0.1414722735505765	0.0	0.0	0.0	0.0
32	0.14731585037749986	0.0	0.0	0.0	0.0
33	0.1528227602267404	0.0	0.0	0.0	2.404764126305907E-5
34	0.15989276675807976	0.0	0.0	0.0	2.404764126305907E-5
35	0.17107491994540225	0.0	0.0	0.0	2.404764126305907E-5
36	0.18158373917735904	0.0	0.0	0.0	2.404764126305907E-5
37	0.18963969900048383	0.0	0.0	0.0	2.404764126305907E-5
38	0.1960123239351945	0.0	0.0	0.0	2.404764126305907E-5
39	0.20339494980295364	0.0	0.0	0.0	2.404764126305907E-5
40	0.21000805115029486	0.0	0.0	0.0	2.404764126305907E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGGA	139400	0.0	44.003586	4
CGTTAAT	115	0.0	44.0	21
CTTATGG	431545	0.0	43.993904	2
ACTTATG	433920	0.0	43.993225	1
TTATGGG	423430	0.0	43.952198	3
TATGGGT	24460	0.0	43.8843	4
TATGGGG	226395	0.0	43.76903	4
ATGGGAG	45505	0.0	43.680035	5
ATGGGAC	26755	0.0	43.67894	5
ATGGGAT	46125	0.0	43.551655	5
ATGGGGT	39765	0.0	43.547596	5
TATGGGC	34330	0.0	43.521122	4
ATGGGGA	63700	0.0	43.411148	5
ATGGGGG	76625	0.0	43.37057	5
ATGGGGC	48185	0.0	43.350834	5
TGGGATA	5750	0.0	42.2	6
ATGGGAA	22990	0.0	42.148327	5
TGGGGGG	30360	0.0	42.083332	6
TGGGATT	17920	0.0	41.66183	6
TGGGGAG	29020	0.0	41.60131	6
>>END_MODULE