FastQCFastQC Report
Fri 17 Jun 2016
SRR1527211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4043972
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA158620.39223812627782784No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC138150.3416195760010208No Hit
ACTTATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG88120.21790457500694863No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT86710.21441790398153104No Hit
ACTTATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA78750.19473428599406722No Hit
ACTTATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC77220.19095087700903962No Hit
ACTTATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC69710.17238002637011335No Hit
ACTTATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG68840.1702286761629408No Hit
ACTTATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG66490.16441755778724482No Hit
ACTTATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC61570.1522513014432345No Hit
ACTTATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC55960.13837880183146667No Hit
ACTTATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA54010.13355680998780406No Hit
ACTTATGGGTGCTGTTTGACACCTCTTTTTAATCTGGTTTTAATTTGCTTTG50930.12594053569114722No Hit
ACTTATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT48100.11894246547701122No Hit
ACTTATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG47390.117186765882652No Hit
ACTTATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT47120.11651910547352949No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT47010.11624709567722032No Hit
ACTTATGGGGAACTTTACAGTTTAAAAGATTAAAAATCATTTCTTAAGTCCT46240.1143430271030561No Hit
ACTTATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG45580.11271096832520106No Hit
ACTTATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT45160.11167238546656603No Hit
ACTTATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG44970.1112025503638502No Hit
ACTTATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG43790.1082846270943518No Hit
ACTTATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT42540.10519360668174754No Hit
ACTTATGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT41700.10311644096447749No Hit
ACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC41450.10249823688195665No Hit
ACTTATGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG41330.10220149892234665No Hit
ACTTATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC40710.10066835279769494No Hit
ACTTATGGGATTTTGTTTTGTCTCGTGATGGTGGTGGTGGTAGGGGTACGCT40690.10061889647109327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTATG4218900.044.001971
CTTATGG4197650.043.984732
TATGGGA1353550.043.9847074
TTATGGG4119050.043.9323163
ATGGGAC253450.043.7585335
TATGGGG2190150.043.7453164
TATGGGC345800.043.6853684
TATGGGT240650.043.667984
ATGGGAT442650.043.6670045
ATGGGAG451050.043.6033675
ATGGGGA607100.043.4313935
ATGGGGC464050.043.3334735
ATGGGGG752450.043.297895
ATGGGGT387900.043.189485
ATGGGAA223750.042.2274865
TGGGATC132550.042.0611086
TGGGGGG299100.041.9013676
TGGGATA51700.041.8181846
TGGGATT173000.041.705786
ATGGGCG58500.041.517955
Produced by FastQC (version 0.11.5)