##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527211_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4043972
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.351151788390226	33.0	31.0	34.0	31.0	34.0
2	32.89883733121792	34.0	31.0	34.0	31.0	34.0
3	32.9872150450102	34.0	31.0	34.0	31.0	34.0
4	36.53420350091445	37.0	37.0	37.0	35.0	37.0
5	36.054198199196236	37.0	35.0	37.0	35.0	37.0
6	36.34954421049404	37.0	37.0	37.0	35.0	37.0
7	36.58099784073678	37.0	37.0	37.0	35.0	37.0
8	36.684138020738025	37.0	37.0	37.0	35.0	37.0
9	38.68409598286041	39.0	39.0	39.0	39.0	39.0
10	38.211933465414695	39.0	39.0	39.0	37.0	39.0
11	37.887700755593755	39.0	38.0	39.0	35.0	39.0
12	37.57179228738478	39.0	37.0	39.0	35.0	39.0
13	37.408889824162976	39.0	37.0	39.0	35.0	39.0
14	38.2573820985902	40.0	38.0	40.0	35.0	40.0
15	38.353029150548025	40.0	38.0	40.0	35.0	40.0
16	38.438306199944016	40.0	38.0	40.0	35.0	40.0
17	38.40772364398171	40.0	38.0	40.0	35.0	40.0
18	38.40631092401233	40.0	38.0	40.0	35.0	40.0
19	38.38047642268542	40.0	38.0	40.0	35.0	40.0
20	38.33218083606909	40.0	38.0	40.0	35.0	40.0
21	34.04245677269773	40.0	35.0	40.0	10.0	40.0
22	34.86899093267708	40.0	35.0	40.0	18.0	40.0
23	36.948819378571365	40.0	35.0	40.0	31.0	40.0
24	37.68345626527582	40.0	37.0	40.0	34.0	40.0
25	37.95088071826412	40.0	38.0	40.0	34.0	40.0
26	38.02796038152589	40.0	38.0	40.0	34.0	40.0
27	37.99821734670764	40.0	38.0	40.0	34.0	40.0
28	37.91016307729134	40.0	38.0	40.0	34.0	40.0
29	37.85917879747931	40.0	38.0	40.0	34.0	40.0
30	37.77916414851537	40.0	37.0	40.0	34.0	40.0
31	37.66771332739198	40.0	37.0	40.0	34.0	40.0
32	37.630186114048264	40.0	37.0	40.0	33.0	40.0
33	37.332895974551754	40.0	36.0	40.0	33.0	40.0
34	37.27005182033901	40.0	36.0	40.0	33.0	40.0
35	37.21331304964525	40.0	36.0	40.0	33.0	40.0
36	37.12892918150769	39.0	36.0	40.0	32.0	40.0
37	37.046777030108025	39.0	35.0	40.0	32.0	40.0
38	36.93128587438291	39.0	35.0	40.0	32.0	40.0
39	36.77359338788696	39.0	35.0	40.0	32.0	40.0
40	31.807892092230116	38.0	31.0	40.0	9.0	40.0
41	32.75400076954044	38.0	31.0	40.0	15.0	40.0
42	34.63017696462784	38.0	32.0	40.0	25.0	40.0
43	35.50875797359625	37.0	34.0	40.0	29.0	40.0
44	36.23550731805265	38.0	35.0	40.0	31.0	40.0
45	36.48416260053235	39.0	35.0	40.0	31.0	40.0
46	36.50343795654371	39.0	35.0	40.0	32.0	40.0
47	36.43638284340248	39.0	35.0	40.0	31.0	40.0
48	36.31328530464603	38.0	35.0	40.0	31.0	40.0
49	36.1621166021921	38.0	35.0	40.0	31.0	40.0
50	36.057267953388404	38.0	35.0	40.0	31.0	40.0
51	35.870631893593725	37.0	35.0	40.0	31.0	40.0
52	35.49295593540212	37.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	8.0
15	19.0
16	58.0
17	153.0
18	335.0
19	665.0
20	1266.0
21	2309.0
22	4075.0
23	6331.0
24	9108.0
25	13193.0
26	18802.0
27	25826.0
28	34456.0
29	47465.0
30	63822.0
31	84985.0
32	121155.0
33	151333.0
34	257164.0
35	369171.0
36	517138.0
37	523145.0
38	1086321.0
39	705669.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.79468206011317	0.17087160840876248	0.022428444113856378	0.01201788736420529
8	99.47870558945512	0.44080423900066573	0.033852855558841655	0.046637315985372796
9	97.89496564268991	1.0552001843731855	0.3781430731963525	0.6716910997405522
10	52.895593738037746	32.7007704306558	6.1005862552955366	8.303049576010913
11	32.77836740709382	23.513713744803376	23.74650467411743	19.96141417398538
12	28.729031753929057	17.10889689641768	27.624795621730318	26.537275727922943
13	24.04309426474763	16.84467647154827	31.449179173347392	27.663050090356712
14	25.375670256866268	18.5475814372602	31.114681308377012	24.96206699749652
15	25.218745332559177	19.042861820012604	29.46091120314384	26.277481644284386
16	29.055666062969777	19.241676252951308	26.62439304723178	25.07826463684714
17	29.160760756998314	18.970507214194363	25.358904562148304	26.50982746665902
18	28.138374845325338	19.239549630907433	25.177152561887173	27.444922961880053
19	27.808847341178428	20.88745916143831	24.379768208088485	26.923925289294782
20	26.679487395066037	21.225740435393718	25.95826578423392	26.13650638530633
21	32.86916922273448	18.817835533975014	25.68709674547697	22.625898497813534
22	28.206303109912728	18.507027249446832	26.662894797491177	26.623774843149263
23	26.792322004207747	20.13666266729839	26.628943029279135	26.44207229921473
24	27.510922429729977	20.401006732984303	25.001285864491646	27.086784972794074
25	26.864478784719577	21.06893915190313	25.450102028401776	26.616480034975513
26	27.01719992126553	20.79050003313574	24.46243445800317	27.729865587595565
27	27.507831409317372	18.81598092172745	25.04540090782033	28.630786761134846
28	28.115748575905076	20.758996353090474	23.42308502630582	27.702170044698626
29	28.492358502976778	20.497743307817164	24.7538805906668	26.256017598539255
30	28.474480040910272	20.140495532610018	24.130978157118793	27.254046269360916
31	29.84098307307766	20.97259822768308	23.53866446157392	25.647754237665342
32	30.90271149256226	20.33095184635304	23.96663478382145	24.799701877263246
33	30.774644334827244	20.68738359217126	24.333254532919614	24.20471754008188
34	28.08350305096079	21.919489056798614	25.934848213588026	24.062159678652574
35	27.826676346918326	23.73626721451088	25.789174603582815	22.647881834987977
36	29.46185087334927	23.09479393032395	23.31754522533786	24.125809970988918
37	29.199237779094418	23.345883700480616	23.342570126598304	24.112308393826666
38	29.632747209921334	22.85814540753497	23.020930906544358	24.48817647599934
39	28.920477194204114	21.979454852803133	22.499735408652683	26.600332544340073
40	27.976059181418666	26.79120923685921	21.89624458329583	23.336486998426302
41	26.314326607602624	21.759720393711927	24.571411473669947	27.354541525015502
42	25.90178665925481	20.563544950360686	25.110559618118028	28.424108772266475
43	25.863977297567835	20.217869955578326	26.656861125645776	27.261291621208063
44	26.059502884787534	20.486566178005187	26.716455999200782	26.737474938006496
45	27.203304078267603	20.16900710489588	25.337366331913273	27.29032248492324
46	26.300157370031247	21.684052214011373	25.2015839872284	26.81420642872898
47	26.255053200170526	22.382993749709442	25.398345982613137	25.963607067506896
48	27.11368921446538	22.170405729812177	25.632472232745428	25.083432822977013
49	26.996403535929524	22.331410801063907	24.390104580348233	26.282081082658337
50	25.45227810677225	22.727036685713948	24.733010020840894	27.087675186672904
51	25.45482510759224	23.522541699101772	24.934519823579393	26.088113369726596
52	27.024470001275976	22.879733094096597	24.38889290034649	25.70690400428094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	19.5
6	39.0
7	577.5
8	1116.0
9	2681.0
10	4246.0
11	5706.5
12	7167.0
13	6766.5
14	6931.0
15	7496.0
16	7923.5
17	8351.0
18	8993.0
19	9635.0
20	11563.0
21	13491.0
22	15481.0
23	17471.0
24	19785.0
25	22099.0
26	26302.0
27	30505.0
28	33348.0
29	36191.0
30	38783.5
31	41376.0
32	51463.5
33	61551.0
34	66748.5
35	71946.0
36	75340.5
37	78735.0
38	85137.0
39	98118.0
40	104697.0
41	114291.0
42	123885.0
43	142083.5
44	160282.0
45	169195.0
46	178108.0
47	196325.0
48	214542.0
49	229935.0
50	245328.0
51	261103.5
52	276879.0
53	305657.5
54	334436.0
55	356572.0
56	378708.0
57	364218.0
58	349728.0
59	347820.5
60	345913.0
61	297914.5
62	249916.0
63	216684.0
64	159866.0
65	136280.0
66	114755.0
67	93230.0
68	81036.0
69	68842.0
70	58495.5
71	48149.0
72	36902.5
73	25656.0
74	17983.0
75	10310.0
76	6716.5
77	3123.0
78	2727.5
79	2332.0
80	1437.0
81	542.0
82	352.0
83	162.0
84	103.0
85	44.0
86	37.5
87	31.0
88	31.5
89	38.0
90	44.0
91	22.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4043972.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.903052752802949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.44039766397046	5.329850204083673
2	10.383588093699103	1.6412408893576123
3	4.543280984345246	1.0771736787026098
4	2.4011429391156436	0.7590543725940501
5	1.4131725071758254	0.5584188436510676
6	0.8730506342636689	0.41398591310723093
7	0.5530059005143486	0.3059304363063338
8	0.40843161216699303	0.258228526149448
9	0.31148093955403505	0.22154852671093492
>10	5.015755805012412	11.043700120526195
>50	3.6534535659766436	20.882417789351937
>100	2.8196672665404687	39.25488316674745
>500	0.11748613610567257	6.263597109000118
>1k	0.061313077280147864	8.928592725205862
>5k	0.004038585928632495	2.2002818009570015
>10k+	7.342883506604535E-4	0.8610958975485083
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	15862	0.39223812627782784	No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	13815	0.3416195760010208	No Hit
ACTTATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	8812	0.21790457500694863	No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8671	0.21441790398153104	No Hit
ACTTATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	7875	0.19473428599406722	No Hit
ACTTATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	7722	0.19095087700903962	No Hit
ACTTATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	6971	0.17238002637011335	No Hit
ACTTATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6884	0.1702286761629408	No Hit
ACTTATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	6649	0.16441755778724482	No Hit
ACTTATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6157	0.1522513014432345	No Hit
ACTTATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	5596	0.13837880183146667	No Hit
ACTTATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	5401	0.13355680998780406	No Hit
ACTTATGGGTGCTGTTTGACACCTCTTTTTAATCTGGTTTTAATTTGCTTTG	5093	0.12594053569114722	No Hit
ACTTATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	4810	0.11894246547701122	No Hit
ACTTATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	4739	0.117186765882652	No Hit
ACTTATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4712	0.11651910547352949	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	4701	0.11624709567722032	No Hit
ACTTATGGGGAACTTTACAGTTTAAAAGATTAAAAATCATTTCTTAAGTCCT	4624	0.1143430271030561	No Hit
ACTTATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	4558	0.11271096832520106	No Hit
ACTTATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	4516	0.11167238546656603	No Hit
ACTTATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	4497	0.1112025503638502	No Hit
ACTTATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	4379	0.1082846270943518	No Hit
ACTTATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	4254	0.10519360668174754	No Hit
ACTTATGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	4170	0.10311644096447749	No Hit
ACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4145	0.10249823688195665	No Hit
ACTTATGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG	4133	0.10220149892234665	No Hit
ACTTATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4071	0.10066835279769494	No Hit
ACTTATGGGATTTTGTTTTGTCTCGTGATGGTGGTGGTGGTAGGGGTACGCT	4069	0.10061889647109327	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.4728163300833933E-5	0.0	0.0	0.0	0.0
8	2.225534697075054E-4	0.0	0.0	0.0	0.0
9	8.654857155291876E-4	0.0	0.0	0.0	0.0
10	0.00660241960132266	0.0	0.0	0.0	0.0
11	0.011053488995472769	0.0	0.0	0.0	0.0
12	0.014391791041085348	0.0	0.0	0.0	0.0
13	0.018818132271934622	0.0	0.0	0.0	0.0
14	0.02653331922179481	0.0	0.0	0.0	0.0
15	0.03283900086350746	0.0	0.0	0.0	0.0
16	0.041271304549091836	0.0	0.0	0.0	0.0
17	0.04646421884226696	0.0	0.0	0.0	0.0
18	0.05126148252262874	0.0	0.0	0.0	0.0
19	0.06088073804665314	0.0	0.0	0.0	0.0
20	0.0671122351984633	0.0	0.0	0.0	0.0
21	0.07322009153376928	0.0	0.0	0.0	0.0
22	0.07967414215528693	0.0	0.0	0.0	0.0
23	0.08617764910340625	0.0	0.0	0.0	0.0
24	0.09379392340006311	0.0	0.0	0.0	0.0
25	0.09886319687673406	0.0	0.0	0.0	0.0
26	0.10462485892582837	0.0	0.0	0.0	0.0
27	0.1101145111786135	0.0	0.0	0.0	0.0
28	0.12025305813195541	0.0	0.0	0.0	0.0
29	0.1251986907921222	0.0	0.0	0.0	0.0
30	0.13113344998432233	0.0	0.0	0.0	0.0
31	0.13726603448292915	0.0	0.0	0.0	0.0
32	0.14310188102192598	0.0	0.0	0.0	0.0
33	0.14794860102888943	0.0	0.0	0.0	0.0
34	0.15452629246691124	0.0	0.0	0.0	0.0
35	0.16424446064413897	0.0	0.0	0.0	0.0
36	0.17233057004351168	0.0	0.0	0.0	0.0
37	0.17908135862463934	0.0	0.0	0.0	0.0
38	0.18511503047004282	0.0	0.0	0.0	0.0
39	0.19082723619253547	0.0	0.0	0.0	0.0
40	0.19896280191850982	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTATG	421890	0.0	44.00197	1
CTTATGG	419765	0.0	43.98473	2
TATGGGA	135355	0.0	43.984707	4
TTATGGG	411905	0.0	43.932316	3
ATGGGAC	25345	0.0	43.758533	5
TATGGGG	219015	0.0	43.745316	4
TATGGGC	34580	0.0	43.685368	4
TATGGGT	24065	0.0	43.66798	4
ATGGGAT	44265	0.0	43.667004	5
ATGGGAG	45105	0.0	43.603367	5
ATGGGGA	60710	0.0	43.431393	5
ATGGGGC	46405	0.0	43.333473	5
ATGGGGG	75245	0.0	43.29789	5
ATGGGGT	38790	0.0	43.18948	5
ATGGGAA	22375	0.0	42.227486	5
TGGGATC	13255	0.0	42.061108	6
TGGGGGG	29910	0.0	41.901367	6
TGGGATA	5170	0.0	41.818184	6
TGGGATT	17300	0.0	41.70578	6
ATGGGCG	5850	0.0	41.51795	5
>>END_MODULE