FastQCFastQC Report
Fri 17 Jun 2016
SRR1527207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381721
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGTCCATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAA22920.6004385401903485No Hit
GGCTGCGGGACTTCTCAAAATAACAAATCAGGATGTCAAAACATTTGTAT20050.5252527369466181No Hit
GGCTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC19750.5173935937504094No Hit
GGCTGCGGGGGTGAGTTCCCTTCTGTGTCTATGTGTGTGTTAGAGTGCTC19520.511368250633316No Hit
GGCTGCGGGAGGATATGTATTGTTTTTTTTCTCCTATAAAATCCACCTAT13620.3568051011078772No Hit
GGCTGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA11520.301791098734416No Hit
GGCTGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT9480.24834892500019645No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG8870.23236866716790536No Hit
GGCTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT8860.23210669572803172No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC8430.22084192381346585No Hit
GGCTGCGGTTGGAGACGGTGGGATGGGAGAAGAGAAGAGTGCGCTGTCCG8370.2192700951742241No Hit
GGCTGCGGGGTGGGTTCGAACAAAGGCGCCATCATCGGACTCATGGTGGG8340.21848418085460325No Hit
GGCTGCGGGATTGAAGAGGGGTCTTCAGGCTCTTTCCCATCACTCTTGCT8030.21036306621852086No Hit
GGCTGCGGGACGGGGGGCTGGGGACTCTCTCTTGCAGTCTGGTCTTACCA7960.20852926613940548No Hit
GGCTGCGGGATTCTCCTGCTTTTTTACCACTGACTAGGAACTGGGTTTGG7870.20617152318054285No Hit
GGCTGCGGGAGAAATAAAAATTTTTAATTAAAATGCTGGCTTTTGCTGTA7700.20171800870269124No Hit
GGCTGCGGGATCCTTAGGATGTAACTTTGAGAGTCCCTTCATGGTTCTGC7170.1878335223893891No Hit
GGCTGCGGGATCTTGTTATATACACAGACACTTTTATGGTCGTTTTGTAT6560.17185326455709798No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG6210.16268426416152112No Hit
GGCTGCGGGGGTAATTAGGACTTCATTCCTCCATGTTTTCTTCCCTTATC6200.1624222927216475No Hit
GGCTGCGGGAGGCCTTAGTGTCACGCAGGTTTATTACAGTGGCATGATGG6150.16111243552227936No Hit
GGCTGCGGGGCTGGCTGCGGGGCTGGCTGCGGGGTTCTTTCTCTTTTCTA6100.15980257832291123No Hit
GGCTGCGGGCAGCACAGTGACCTGGACAAGTCTTCTATCCACAGCTACTG6050.15849272112354312No Hit
GGCTGCGGGTGCAGTGCTGGCTGCGGGTCGTGTATAAAATCAAACTGAAG5910.15482512096531237No Hit
GGCTGCGGGATCTGTCCACTTTGCGATCCCAATTCTGTCCCAATCTCACC5310.13910683457289486No Hit
GGCTGCGGGGATACCACACCCAATGAACTTGTTCAGTTAAATAAGTTATT5300.13884486313302122No Hit
GGCTGCGGGGCTGGCTGCGGGACAGGGGTGCTTGAGTCTGGCCTCAGTCG5290.1385828916931476No Hit
GGCTGCGGGATTGTGTGAACATTTTGTTCCTTAGTTTCTTGTTCTTATGA5280.138320920253274No Hit
GGCTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC5110.13386740577542236No Hit
GGCTGCGGGCAGTGCTGGCTGCGGGATATATCAATGCTGTGTGTACGTGC5020.13150966281655974No Hit
GGCTGCGGGGGTCTTCACACAGTGCTTGTTACTATTTGTCCATAAAATAT4980.13046177705706524No Hit
GGCTGCGGGGCTGCGGGAAACTCCAGGACTCTTCTACCCAGCTCCTGCCT4830.12653220545896085No Hit
GGCTGCGGGCTCTAACTACAGGAAATGGCAGGGTTTTTTGTGTTTTGTTT4760.12469840537984549No Hit
GGCTGCGGGACCATCGCCTTCCCTTTTCTCCTGCACCCCTGTAGTCTACC4610.12076883378174111No Hit
GGCTGCGGGCTGGCTGCGGGGCTTTTGTGACTGGCGTGAACGAGTGCGCA4590.12024489090199386No Hit
GGCTGCGGGGATGAAGTGGTTTCTGTCATCTTACATTTCACTGTCCTGCT4560.11945897658237299No Hit
GGCTGCGGGGTGGCTTTCTTCTGTTTTCAAGAAGAGTTCCATATGCTGGA4530.11867306226275212No Hit
GGCTGCGGGGTGCGCCTGCTCTGCACACACAGGCGCGGTGCTGCTCACTC4510.11814911938300486No Hit
GGCTGCGGCCTCAACAGGACCAGGCCTATCTGGGATCACCTCTTTACCCA4510.11814911938300486No Hit
GGCTGCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCT4480.11736320506338399No Hit
GGCTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT4400.115267433544395No Hit
GGCTGCGGGGGGGTTGAAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC4400.115267433544395No Hit
GGCTGCGGGGTGCAGTGCTGGCTGCGGGATCTCAGAATAACTATGGATTT4360.11421954778490048No Hit
GGCTGCGGGATTTGGAGTTCTGTAGGTTTCTTGTATGTTTCTTTAGGTCT4340.11369560490515324No Hit
GGCTGCGGGGTTCTGGTTCACAGTCATCACCGTCTCCTTCGGCTACTACA4300.11264771914565874No Hit
GGCTGCGGGGCTGGCTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGAT4260.11159983338616423No Hit
GGCTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT4260.11159983338616423No Hit
GGCTGCGGGCTCCCCACCCTGCCTTCTGAGGGGCACGCAGGGCGGGGTCT4250.11133786194629063No Hit
GGCTGCGGGAACTTTTGAGTGTCTCCAGTTTCCTAAAGGACACTGGACTC4240.111075890506417No Hit
GGCTGCGGGGTTGTCTTTGAAATGTATATTTTGTCATTTTAAATTCATTT4240.111075890506417No Hit
GGCTGCGGGGAAGTTTTAGGGGAAGGAAACGTTTAGTAGTAATGGCAACA4180.10950406186717523No Hit
GGCTGCGGGGGGCACTCCGAGCCGATGCCACCGGCCCGCGGGTCTCTGAA4140.10845617610768074No Hit
GGCTGCGGGATGAACATAGTGGAGCATGTGTCCTTATTACATGTTGGAGC4130.10819420466780712No Hit
GGCTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT4090.10714631890831262No Hit
GGCTGCGGGGCTGGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG4050.10609843314881812No Hit
GGCTGCGGGGAGGGGCCGAAGCCGGGGCTGGAGTAGGTGGCGGAAGGACA4050.10609843314881812No Hit
GGCTGCGGGCTTTGACGACTCTGTATTTCATTTTTGAGACTGTATTCCCC4010.10505054738932362No Hit
GGCTGCGGGGTCGCCCTGGGTAGAGTTTATTTTTTTGATGGCTAAGCTGT4000.10478857594945No Hit
GGCTGCGGGAGAGTCCTGTGCTTTGGGAGGCTCCCTCACGGCTGTCCACA3930.10295477587033462No Hit
GGCTGCGGGATTGATTCTGTACTTAAGGATTCATAACGTCCTCCCTTCCC3910.10243083299058738No Hit
GGCTGCGGGGACAAGGCAACCACAGAAGTCAGAACAATCAGGCTTGAGTG3890.10190689011084011No Hit
GGCTGCGGGGAGACCATGGAGGAGAAGAAAGCATTTATGGGACCACTCAA3880.10164491867096649No Hit
GGCTGCGGGAATCCCAGCACTTGGAAAGCAGATTTCTGAGTTTGAGGCCA3870.10138294723109285No Hit
GGCTGCGGGATCAGCAGGCTGAAGGAGCAGCTGAAAGCAGCCACAGAGGC3870.10138294723109285No Hit
GGCTGCGGGACCGGCCATTTCCTTTAGCTCCCTCAAGGCTCTTGGGGAGT3860.10112097579121923No Hit
GGCTGCGGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.100597032911472No Hit
GGCTGCGGGCTGACAGCATCATGTGTGTCAACCTGTGTCAATTATGATGG3830.10033506147159837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTT650.044.00000440
AACCGCG650.044.00000411
TCGTTGC551.8189894E-1244.00000442
ACACGCT650.044.00000412
CGTGCGT551.8189894E-1244.00000416
GGATCGA650.044.0000048
TACGGAA650.044.00000437
TGACGCC650.044.00000432
GACTTGT551.8189894E-1244.00000431
ATTCCCG551.8189894E-1244.00000432
TAACAAA2200.044.00000421
CCATATC650.044.00000440
CGAGCCG551.8189894E-1244.00000418
CCGAAGC551.8189894E-1244.00000416
AGTAGGT551.8189894E-1244.00000432
GCTCGTT551.8189894E-1244.00000440
CGAAGCC551.8189894E-1244.00000417
ACGCGAC254.437236E-544.020
TCCGGAC207.849867E-444.014
TCGCCTA254.437236E-544.013
Produced by FastQC (version 0.11.5)