FastQCFastQC Report
Fri 17 Jun 2016
SRR1527206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421525
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA23530.5582112567463376No Hit
GGCTGCGGGGTCCATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAG22090.5240495818753337No Hit
GGCTGCGGGGGTGAGTTCCCTTCTGTGTCTATGTGTGTGTTAGAGTGCTCAT19690.46711345709032676No Hit
GGCTGCGGGACTTCTCAAAATAACAAATCAGGATGTCAAAACATTTGTATAG19230.45620069983986716No Hit
GGCTGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG12770.30294763062689045No Hit
GGCTGCGGGAGGATATGTATTGTTTTTTTTCTCCTATAAAATCCACCTATTT12700.3012869936539944No Hit
GGCTGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT9430.22371152363442262No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT8440.20022537216060735No Hit
GGCTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC8360.1983275013344404No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC8290.1966668643615444No Hit
GGCTGCGGGAGAAATAAAAATTTTTAATTAAAATGCTGGCTTTTGCTGTATG8240.1954806950951901No Hit
GGCTGCGGTTGGAGACGGTGGGATGGGAGAAGAGAAGAGTGCGCTGTCCGAG7820.18551687325781388No Hit
GGCTGCGGGACGGGGGGCTGGGGACTCTCTCTTGCAGTCTGGTCTTACCAAG7710.1829073008718344No Hit
GGCTGCGGGATCCTTAGGATGTAACTTTGAGAGTCCCTTCATGGTTCTGCAC7250.17199454362137476No Hit
GGCTGCGGGATTGAAGAGGGGTCTTCAGGCTCTTTCCCATCACTCTTGCTGC7160.169859438941937No Hit
GGCTGCGGGGTGGGTTCGAACAAAGGCGCCATCATCGGACTCATGGTGGGCG7120.16891050352885356No Hit
GGCTGCGGGATTCTCCTGCTTTTTTACCACTGACTAGGAACTGGGTTTGGCC6790.16108178637091514No Hit
GGCTGCGGGATCTTGTTATATACACAGACACTTTTATGGTCGTTTTGTATTG6650.15776051242512307No Hit
GGCTGCGGGCAGCACAGTGACCTGGACAAGTCTTCTATCCACAGCTACTGAG6290.14922009370737205No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT6100.14471265049522566No Hit
GGCTGCGGGGCTGGCTGCGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCA5990.1421030781092462No Hit
GGCTGCGGGGATACCACACCCAATGAACTTGTTCAGTTAAATAAGTTATTCT5790.13735840104382896No Hit
GGCTGCGGGTGCAGTGCTGGCTGCGGGTCGTGTATAAAATCAAACTGAAGGG5780.13712116719055809No Hit
GGCTGCGGGATCTGTCCACTTTGCGATCCCAATTCTGTCCCAATCTCACCAG5700.1352232963643912No Hit
GGCTGCGGGGCTGGCTGCGGGACAGGGGTGCTTGAGTCTGGCCTCAGTCGCT5230.1240733052606607No Hit
GGCTGCGGGAGGCCTTAGTGTCACGCAGGTTTATTACAGTGGCATGATGGTG5080.12051479746159777No Hit
GGCTGCGGGCTCCCCACCCTGCCTTCTGAGGGGCACGCAGGGCGGGGTCTCA5060.12004032975505605No Hit
GGCTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT4890.11600735424945138No Hit
GGCTGCGGGGATGAAGTGGTTTCTGTCATCTTACATTTCACTGTCCTGCTCA4780.11339778186347192No Hit
GGCTGCGGCCTCAACAGGACCAGGCCTATCTGGGATCACCTCTTTACCCAGG4770.11316054801020106No Hit
GGCTGCGGGGCTGCGGGAAACTCCAGGACTCTTCTACCCAGCTCCTGCCTGC4760.1129233141569302No Hit
GGCTGCGGGCTGGCTGCGGGGCTTTTGTGACTGGCGTGAACGAGTGCGCAGT4710.1117371448905759No Hit
GGCTGCGGGATTGTGTGAACATTTTGTTCCTTAGTTTCTTGTTCTTATGAGA4620.10960204021113813No Hit
GGCTGCGGGCTCTAACTACAGGAAATGGCAGGTTTTTTTGTGTTTTGTTTTT4620.10960204021113813No Hit
GGCTGCGGGGCTGGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG4590.10889033865132554No Hit
GGCTGCGGGATGAACATAGTGGAGCATGTGTCCTTATTACATGTTGGAGCAT4570.10841587094478382No Hit
GGCTGCGGGGACAAGGCAACCACAGAAGTCAGAACAATCAGGCTTGAGTGGG4510.10699246782515864No Hit
GGCTGCGGGACATGTGTGAGCACCATTGTGGCTAGTAAGCCAATGTACCGGG4510.10699246782515864No Hit
GGCTGCGGGGCTGGCTGCGGGGCTGGCTGCGGGGTTCTTTCTCTTTTCTATA4480.10628076626534605No Hit
GGCTGCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGA4480.10628076626534605No Hit
GGCTGCGGGATCAGCAGGCTGAAGGAGCAGCTGAAAGCAGCCACAGAGGCAC4360.10343396002609573No Hit
GGCTGCGGGACCATCGCCTTCCCTTTTCTCCTGCACCCCTGTAGTCTACCTC4340.102959492319554No Hit
GGCTGCGGGGCTGGCTGCGGGGTCTTTGCTTCTGTCGCTCATCGCAGACACC4330.10272225846628313No Hit
GGCTGCGGGGCTGGCTGCGGGGGAACCTTTTCACGGCCACTCTGCAGGTCTG4330.10272225846628313No Hit
GGCTGCGGGGTTCTGGTTCACAGTCATCACCGTCTCCTTCGGCTACTACACG4290.1017733230531997No Hit
GGCTGCGGGCAGTGCTGGCTGCGGGATATATCAATGCTGTGTGTACGTGCAG4260.1010616214933871No Hit
GGCTGCGGGGCTGGCTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCG4250.10082438764011624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAC351.0175063E-746.00000435
CTCACAT351.0175063E-746.00000441
ACACGCT351.0175063E-746.00000412
CTGTACG351.0175063E-746.00000446
CGTGCTA351.0175063E-746.00000420
CCTAGGG351.0175063E-746.00000422
TAGACGA351.0175063E-746.00000429
GGCACGA351.0175063E-746.00000434
AACTTCG351.0175063E-746.00000431
CGTTGAC351.0175063E-746.00000421
ACATTCG351.0175063E-746.00000410
ATAGCCG351.0175063E-746.00000441
GCACGAC351.0175063E-746.00000435
GCGCCAA700.046.0000049
TATATCA700.046.00000427
ATTCGAG351.0175063E-746.00000412
CACGACA351.0175063E-746.00000436
ATGTCGC351.0175063E-746.00000423
CCTCGCA351.0175063E-746.00000425
AAAACGT351.0175063E-746.00000421
Produced by FastQC (version 0.11.5)