##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527206_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421525
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48371270980369	34.0	31.0	34.0	31.0	34.0
2	32.86192040804223	34.0	31.0	34.0	31.0	34.0
3	32.8936789039796	34.0	31.0	34.0	31.0	34.0
4	36.31788861870589	37.0	37.0	37.0	35.0	37.0
5	36.208734950477435	37.0	37.0	37.0	35.0	37.0
6	36.095427317478205	37.0	37.0	37.0	35.0	37.0
7	36.43964177688156	37.0	37.0	37.0	35.0	37.0
8	36.58391080007117	37.0	37.0	37.0	35.0	37.0
9	38.670503528853565	39.0	39.0	39.0	38.0	39.0
10	37.93595397663247	39.0	38.0	39.0	35.0	39.0
11	37.52348496530455	39.0	37.0	39.0	35.0	39.0
12	37.086020995196016	39.0	37.0	39.0	34.0	39.0
13	37.04722851550916	39.0	37.0	39.0	33.0	39.0
14	37.85080600201649	40.0	38.0	40.0	33.0	40.0
15	37.69758614554297	40.0	38.0	40.0	33.0	40.0
16	32.65413676531641	39.0	33.0	40.0	3.0	40.0
17	34.181526599845796	39.0	33.0	40.0	16.0	40.0
18	36.00816321689105	39.0	33.0	40.0	30.0	40.0
19	36.97254255382243	39.0	35.0	40.0	31.0	40.0
20	37.435381056876814	39.0	37.0	40.0	33.0	40.0
21	37.560073542494514	39.0	37.0	40.0	33.0	40.0
22	37.20615859083091	39.0	36.0	40.0	32.0	40.0
23	36.990989858252775	39.0	35.0	40.0	32.0	40.0
24	36.92185516873258	39.0	35.0	40.0	32.0	40.0
25	36.900039143585786	39.0	35.0	40.0	32.0	40.0
26	36.73213451159481	38.0	35.0	40.0	32.0	40.0
27	36.583282130360004	38.0	35.0	40.0	31.0	40.0
28	36.37127335270743	38.0	35.0	40.0	31.0	40.0
29	36.112811814245894	38.0	35.0	40.0	31.0	40.0
30	35.97454243520551	38.0	35.0	40.0	30.0	40.0
31	35.92135460530218	37.0	34.0	40.0	31.0	40.0
32	35.864736373880554	37.0	34.0	40.0	31.0	40.0
33	35.38753810568768	37.0	34.0	40.0	30.0	40.0
34	35.30866496649072	37.0	34.0	40.0	29.0	40.0
35	35.158635905343694	36.0	34.0	40.0	29.0	40.0
36	35.125933218670305	36.0	34.0	40.0	29.0	40.0
37	34.825939149516635	36.0	33.0	39.0	28.0	40.0
38	34.816715497301466	36.0	33.0	39.0	29.0	40.0
39	34.360773382361664	35.0	33.0	39.0	27.0	40.0
40	34.24297965719708	35.0	33.0	39.0	26.0	40.0
41	34.19847458632347	35.0	33.0	39.0	26.0	40.0
42	34.08812525947453	35.0	33.0	39.0	26.0	40.0
43	33.94588458573038	35.0	33.0	39.0	26.0	40.0
44	34.424852618468655	35.0	33.0	39.0	28.0	40.0
45	34.54703754225728	35.0	34.0	39.0	28.0	40.0
46	34.48980250281715	35.0	34.0	39.0	28.0	40.0
47	34.16014945732756	35.0	33.0	39.0	27.0	40.0
48	34.158351224719766	35.0	33.0	39.0	27.0	40.0
49	34.18674099994069	35.0	33.0	39.0	28.0	40.0
50	33.667319850542675	35.0	33.0	38.0	26.0	40.0
51	33.78367831089496	35.0	33.0	38.0	26.0	40.0
52	33.43497064231066	35.0	33.0	37.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	4.0
16	18.0
17	32.0
18	75.0
19	158.0
20	301.0
21	520.0
22	784.0
23	1193.0
24	1781.0
25	2213.0
26	3146.0
27	4246.0
28	5551.0
29	7476.0
30	10007.0
31	13665.0
32	18756.0
33	27069.0
34	40818.0
35	55506.0
36	65118.0
37	65184.0
38	80336.0
39	17566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.01381887195303	0.4780262143407864	0.301998695213807	0.2061562184923789
8	99.1295889923492	0.4078049937726113	0.3501571674277919	0.11244884645038847
9	96.69817923017615	1.3648063578672676	1.0008896269497656	0.9361247850068205
10	51.34286222643971	33.52399027341201	3.964414922009371	11.1687325781389
11	26.471027815669295	18.081489828598542	28.735899412846212	26.711582942885947
12	27.36136646699484	13.092461894312319	35.821837376193585	23.72433426249926
13	34.829013700255025	15.172053852084694	28.567937844730444	21.430994602929836
14	34.42595338354783	17.89905699543325	25.124251230650614	22.550738390368306
15	23.783879959670244	17.741296483008124	28.582883577486506	29.891939979835126
16	27.84342565684123	26.500919281181424	29.46112330229524	16.194531759682107
17	38.08647173951723	18.038076033449972	21.482474349089614	22.39297787794318
18	26.919637032204495	19.89561710456082	22.502817152007594	30.68192871122709
19	35.916256449795384	20.606606962813593	22.096435561354603	21.380701026036416
20	34.06939090208173	21.73394223355673	24.243164699602634	19.95350216475891
21	35.99122234742898	20.76199513670601	25.2554415515094	17.991340964355615
22	28.027993594685963	22.84419666686436	27.216416582646342	21.91139315580333
23	27.273827175137892	22.71988612775043	28.548959136468778	21.457327560642902
24	26.16618231421624	23.940454302829014	28.135934997924206	21.757428385030543
25	28.848348259296603	24.69319731925746	25.57807959195777	20.88037482948817
26	25.067315105865607	25.113338473400155	26.84941581163632	22.96993060909792
27	23.95302769705237	23.722910859379635	28.450507087361366	23.87355435620663
28	26.323705592788095	23.959907478797223	26.831623272641004	22.88476365577368
29	25.901192100112684	22.732933989680326	26.575647944961744	24.79022596524524
30	24.594982504003323	22.88144238182789	28.7067196488939	23.816855465274894
31	26.479093766680506	22.14174722732934	28.781211078820945	22.59794792716921
32	27.711286400569364	22.573987307988848	28.301286993653996	21.413439297787797
33	26.98131783405492	24.025858490006524	28.802324891762055	20.1904987841765
34	24.32738271751379	24.595694205563134	31.17988256924263	19.897040507680448
35	25.507621137536322	26.17662060376016	30.192989739635845	18.12276851906767
36	24.274005100527845	27.112745388766978	25.79514856770061	22.818100943004566
37	25.61295296838859	25.835715556609927	27.15212620840994	21.399205266591544
38	25.08060020164877	26.083624933277978	26.92343277385683	21.912342091216416
39	26.442796987130063	26.355257695273117	24.61087717217247	22.59106814542435
40	25.39825633117846	24.672795207876163	26.55097562422158	23.3779728367238
41	23.508925923729315	25.26137239784117	26.5768341142281	24.65286756420141
42	22.952612537809145	24.744202597710693	29.279876638396303	23.023308226083863
43	23.48188126445644	23.982681928711227	28.400925212027754	24.13451159480458
44	24.82889508332839	24.202597710693315	27.693493861574048	23.275013344404247
45	24.14305201352233	25.53893600616808	27.098274123717452	23.219737856592136
46	24.321689105035286	23.390071763240613	28.5259474527015	23.762291679022596
47	23.825395883992645	26.11493980190973	27.655773678903977	22.403890635193644
48	22.68074254196074	26.707312733527072	25.929422928651917	24.682521795860268
49	23.210722970167843	25.07395765375719	27.349267540478028	24.366051835596938
50	21.977106933159362	25.333965956942055	28.300812525947457	24.38811458395113
51	23.834410770416937	27.70155981258526	25.388529743194354	23.075499673803453
52	25.553170037364332	24.766028112211615	27.06007947334085	22.62072237708321
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	27.5
12	55.0
13	34.5
14	134.5
15	255.0
16	688.5
17	1122.0
18	1408.0
19	1694.0
20	1695.5
21	1697.0
22	2197.0
23	2697.0
24	2434.5
25	2172.0
26	2151.5
27	2131.0
28	2701.5
29	3272.0
30	2882.5
31	2493.0
32	2772.5
33	3052.0
34	4721.0
35	6390.0
36	7049.5
37	7709.0
38	8130.0
39	8835.5
40	9120.0
41	10021.5
42	10923.0
43	11615.5
44	12308.0
45	12982.0
46	13656.0
47	14546.0
48	15436.0
49	16049.5
50	16663.0
51	17550.0
52	18437.0
53	20793.5
54	23150.0
55	23402.0
56	23654.0
57	25018.0
58	26382.0
59	27358.0
60	28334.0
61	30031.0
62	31728.0
63	31235.0
64	28634.5
65	26527.0
66	25405.0
67	24283.0
68	21986.5
69	19690.0
70	18138.5
71	16587.0
72	14035.0
73	11483.0
74	9873.0
75	8263.0
76	6224.5
77	4186.0
78	4048.0
79	3910.0
80	2878.0
81	1846.0
82	1154.5
83	463.0
84	418.0
85	373.0
86	216.0
87	59.0
88	34.0
89	5.5
90	2.0
91	2.0
92	2.0
93	3.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	421525.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.369076567226143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.72967313766547	9.455904157523278
2	10.473080881570075	2.800308404009252
3	4.260567129218867	1.7087954451100174
4	2.258934592043156	1.207994780855228
5	1.4000780778649253	0.9358875511535495
6	1.0132377470986975	0.8127631813059724
7	0.7665826738119743	0.717395172291086
8	0.5571920360577776	0.5959314394164047
9	0.4400752386698371	0.5295059605005634
>10	4.4255953437200555	13.441432892473756
>50	1.7869184086311531	17.406085048336397
>100	1.8401533165347626	44.35537631219975
>500	0.03726443553252653	3.422810034991993
>1k	0.010646981580721866	2.6098096198327503
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2353	0.5582112567463376	No Hit
GGCTGCGGGGTCCATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAG	2209	0.5240495818753337	No Hit
GGCTGCGGGGGTGAGTTCCCTTCTGTGTCTATGTGTGTGTTAGAGTGCTCAT	1969	0.46711345709032676	No Hit
GGCTGCGGGACTTCTCAAAATAACAAATCAGGATGTCAAAACATTTGTATAG	1923	0.45620069983986716	No Hit
GGCTGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1277	0.30294763062689045	No Hit
GGCTGCGGGAGGATATGTATTGTTTTTTTTCTCCTATAAAATCCACCTATTT	1270	0.3012869936539944	No Hit
GGCTGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	943	0.22371152363442262	No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	844	0.20022537216060735	No Hit
GGCTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	836	0.1983275013344404	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	829	0.1966668643615444	No Hit
GGCTGCGGGAGAAATAAAAATTTTTAATTAAAATGCTGGCTTTTGCTGTATG	824	0.1954806950951901	No Hit
GGCTGCGGTTGGAGACGGTGGGATGGGAGAAGAGAAGAGTGCGCTGTCCGAG	782	0.18551687325781388	No Hit
GGCTGCGGGACGGGGGGCTGGGGACTCTCTCTTGCAGTCTGGTCTTACCAAG	771	0.1829073008718344	No Hit
GGCTGCGGGATCCTTAGGATGTAACTTTGAGAGTCCCTTCATGGTTCTGCAC	725	0.17199454362137476	No Hit
GGCTGCGGGATTGAAGAGGGGTCTTCAGGCTCTTTCCCATCACTCTTGCTGC	716	0.169859438941937	No Hit
GGCTGCGGGGTGGGTTCGAACAAAGGCGCCATCATCGGACTCATGGTGGGCG	712	0.16891050352885356	No Hit
GGCTGCGGGATTCTCCTGCTTTTTTACCACTGACTAGGAACTGGGTTTGGCC	679	0.16108178637091514	No Hit
GGCTGCGGGATCTTGTTATATACACAGACACTTTTATGGTCGTTTTGTATTG	665	0.15776051242512307	No Hit
GGCTGCGGGCAGCACAGTGACCTGGACAAGTCTTCTATCCACAGCTACTGAG	629	0.14922009370737205	No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	610	0.14471265049522566	No Hit
GGCTGCGGGGCTGGCTGCGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCA	599	0.1421030781092462	No Hit
GGCTGCGGGGATACCACACCCAATGAACTTGTTCAGTTAAATAAGTTATTCT	579	0.13735840104382896	No Hit
GGCTGCGGGTGCAGTGCTGGCTGCGGGTCGTGTATAAAATCAAACTGAAGGG	578	0.13712116719055809	No Hit
GGCTGCGGGATCTGTCCACTTTGCGATCCCAATTCTGTCCCAATCTCACCAG	570	0.1352232963643912	No Hit
GGCTGCGGGGCTGGCTGCGGGACAGGGGTGCTTGAGTCTGGCCTCAGTCGCT	523	0.1240733052606607	No Hit
GGCTGCGGGAGGCCTTAGTGTCACGCAGGTTTATTACAGTGGCATGATGGTG	508	0.12051479746159777	No Hit
GGCTGCGGGCTCCCCACCCTGCCTTCTGAGGGGCACGCAGGGCGGGGTCTCA	506	0.12004032975505605	No Hit
GGCTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	489	0.11600735424945138	No Hit
GGCTGCGGGGATGAAGTGGTTTCTGTCATCTTACATTTCACTGTCCTGCTCA	478	0.11339778186347192	No Hit
GGCTGCGGCCTCAACAGGACCAGGCCTATCTGGGATCACCTCTTTACCCAGG	477	0.11316054801020106	No Hit
GGCTGCGGGGCTGCGGGAAACTCCAGGACTCTTCTACCCAGCTCCTGCCTGC	476	0.1129233141569302	No Hit
GGCTGCGGGCTGGCTGCGGGGCTTTTGTGACTGGCGTGAACGAGTGCGCAGT	471	0.1117371448905759	No Hit
GGCTGCGGGATTGTGTGAACATTTTGTTCCTTAGTTTCTTGTTCTTATGAGA	462	0.10960204021113813	No Hit
GGCTGCGGGCTCTAACTACAGGAAATGGCAGGTTTTTTTGTGTTTTGTTTTT	462	0.10960204021113813	No Hit
GGCTGCGGGGCTGGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	459	0.10889033865132554	No Hit
GGCTGCGGGATGAACATAGTGGAGCATGTGTCCTTATTACATGTTGGAGCAT	457	0.10841587094478382	No Hit
GGCTGCGGGGACAAGGCAACCACAGAAGTCAGAACAATCAGGCTTGAGTGGG	451	0.10699246782515864	No Hit
GGCTGCGGGACATGTGTGAGCACCATTGTGGCTAGTAAGCCAATGTACCGGG	451	0.10699246782515864	No Hit
GGCTGCGGGGCTGGCTGCGGGGCTGGCTGCGGGGTTCTTTCTCTTTTCTATA	448	0.10628076626534605	No Hit
GGCTGCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGA	448	0.10628076626534605	No Hit
GGCTGCGGGATCAGCAGGCTGAAGGAGCAGCTGAAAGCAGCCACAGAGGCAC	436	0.10343396002609573	No Hit
GGCTGCGGGACCATCGCCTTCCCTTTTCTCCTGCACCCCTGTAGTCTACCTC	434	0.102959492319554	No Hit
GGCTGCGGGGCTGGCTGCGGGGTCTTTGCTTCTGTCGCTCATCGCAGACACC	433	0.10272225846628313	No Hit
GGCTGCGGGGCTGGCTGCGGGGGAACCTTTTCACGGCCACTCTGCAGGTCTG	433	0.10272225846628313	No Hit
GGCTGCGGGGTTCTGGTTCACAGTCATCACCGTCTCCTTCGGCTACTACACG	429	0.1017733230531997	No Hit
GGCTGCGGGCAGTGCTGGCTGCGGGATATATCAATGCTGTGTGTACGTGCAG	426	0.1010616214933871	No Hit
GGCTGCGGGGCTGGCTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCG	425	0.10082438764011624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0023723385327086175	0.0	0.0	0.0	0.0
11	0.004981910918688097	0.0	0.0	0.0	0.0
12	0.010201055690647056	0.0	0.0	0.0	0.0
13	0.01897870826166894	0.0	0.0	0.0	0.0
14	0.027281893126149103	0.0	0.0	0.0	0.0
15	0.03392444101773323	0.0	0.0	0.0	0.0
16	0.058596761757902856	0.0	0.0	0.0	0.0
17	0.06689994662238301	0.0	0.0	0.0	0.0
18	0.07662653460648834	0.0	0.0	0.0	0.0
19	0.08848822727003143	0.0	0.0	0.0	0.0
20	0.09323290433544867	0.0	0.0	0.0	0.0
21	0.10011268608030366	0.0	0.0	0.0	0.0
22	0.10746693553170038	0.0	0.0	0.0	0.0
23	0.11268608030365933	0.0	0.0	0.0	0.0
24	0.1240733052606607	0.0	0.0	0.0	0.0
25	0.13142755471205742	0.0	0.0	0.0	0.0
26	0.13949350572326671	0.0	0.0	0.0	0.0
27	0.1506434968269972	0.0	0.0	0.0	0.0
28	0.1601328509578317	0.0	0.0	0.0	0.0
29	0.16653816499614496	0.0	0.0	0.0	0.0
30	0.17389241444754167	0.0	0.0	0.0	0.0
31	0.1843307039914596	0.0	0.0	0.0	0.0
32	0.19002431646996026	0.0	0.0	0.0	0.0
33	0.19880196904098216	0.0	0.0	0.0	0.0
34	0.20686792005219146	0.0	0.0	0.0	0.0
35	0.2194413142755471	0.0	0.0	0.0	0.0
36	0.23035407152600676	0.0	0.0	0.0	0.0
37	0.23889449024375778	0.0	0.0	0.0	0.0
38	0.24672320740169623	0.0	0.0	0.0	0.0
39	0.26072000474467705	0.0	0.0	0.0	0.0
40	0.26926042346242807	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAC	35	1.0175063E-7	46.000004	35
CTCACAT	35	1.0175063E-7	46.000004	41
ACACGCT	35	1.0175063E-7	46.000004	12
CTGTACG	35	1.0175063E-7	46.000004	46
CGTGCTA	35	1.0175063E-7	46.000004	20
CCTAGGG	35	1.0175063E-7	46.000004	22
TAGACGA	35	1.0175063E-7	46.000004	29
GGCACGA	35	1.0175063E-7	46.000004	34
AACTTCG	35	1.0175063E-7	46.000004	31
CGTTGAC	35	1.0175063E-7	46.000004	21
ACATTCG	35	1.0175063E-7	46.000004	10
ATAGCCG	35	1.0175063E-7	46.000004	41
GCACGAC	35	1.0175063E-7	46.000004	35
GCGCCAA	70	0.0	46.000004	9
TATATCA	70	0.0	46.000004	27
ATTCGAG	35	1.0175063E-7	46.000004	12
CACGACA	35	1.0175063E-7	46.000004	36
ATGTCGC	35	1.0175063E-7	46.000004	23
CCTCGCA	35	1.0175063E-7	46.000004	25
AAAACGT	35	1.0175063E-7	46.000004	21
>>END_MODULE