SRR1527205_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527205_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	407500
Sequences flagged as poor quality	0
Sequence length	52
%GC	56

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2185	0.5361963190184049	No Hit
GGCTGCGGGGTCCATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAG	2109	0.5175460122699387	No Hit
GGCTGCGGGACTTCTCAAAATAACAAATCAGGATGTCAAAACATTTGTATAG	1947	0.4777914110429448	No Hit
GGCTGCGGGGGTGAGTTCCCTTCTGTGTCTATGTGTGTGTTAGAGTGCTCAT	1900	0.4662576687116564	No Hit
GGCTGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1273	0.31239263803680983	No Hit
GGCTGCGGGAGGATATGTATTGTTTTTTTTCTCCTATAAAATCCACCTATTT	1247	0.30601226993865027	No Hit
GGCTGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	905	0.22208588957055214	No Hit
GGCTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	884	0.2169325153374233	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	848	0.20809815950920246	No Hit
GGCTGCGGTTGGAGACGGTGGGATGGGAGAAGAGAAGAGTGCGCTGTCCGAG	840	0.2061349693251534	No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	824	0.2022085889570552	No Hit
GGCTGCGGGATTCTCCTGCTTTTTTACCACTGACTAGGAACTGGGTTTGGCC	742	0.18208588957055216	No Hit
GGCTGCGGGATTGAAGAGGGGTCTTCAGGCTCTTTCCCATCACTCTTGCTGC	715	0.17546012269938652	No Hit
GGCTGCGGGACGGGGGGCTGGGGACTCTCTCTTGCAGTCTGGTCTTACCAAG	711	0.17447852760736196	No Hit
GGCTGCGGGATCTTGTTATATACACAGACACTTTTATGGTCGTTTTGTATTG	689	0.16907975460122698	No Hit
GGCTGCGGGGTGGGTTCGAACAAAGGCGCCATCATCGGACTCATGGTGGGCG	667	0.16368098159509203	No Hit
GGCTGCGGGAGAAATAAAAATTTTTAATTAAAATGCTGGCTTTTGCTGTATG	666	0.1634355828220859	No Hit
GGCTGCGGGATCCTTAGGATGTAACTTTGAGAGTCCCTTCATGGTTCTGCAC	663	0.16269938650306748	No Hit
GGCTGCGGGGCTGGCTGCGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCA	631	0.15484662576687117	No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	595	0.1460122699386503	No Hit
GGCTGCGGGTGCAGTGCTGGCTGCGGGTCGTGTATAAAATCAAACTGAAGGG	562	0.13791411042944787	No Hit
GGCTGCGGGATCTGTCCACTTTGCGATCCCAATTCTGTCCCAATCTCACCAG	536	0.13153374233128834	No Hit
GGCTGCGGGGATACCACACCCAATGAACTTGTTCAGTTAAATAAGTTATTCT	533	0.13079754601226992	No Hit
GGCTGCGGGGCTGGCTGCGGGACAGGGGTGCTTGAGTCTGGCCTCAGTCGCT	532	0.1305521472392638	No Hit
GGCTGCGGGCAGCACAGTGACCTGGACAAGTCTTCTATCCACAGCTACTGAG	514	0.1261349693251534	No Hit
GGCTGCGGGCTGGCTGCGGGGCTTTTGTGACTGGCGTGAACGAGTGCGCAGT	503	0.1234355828220859	No Hit
GGCTGCGGGGATGAAGTGGTTTCTGTCATCTTACATTTCACTGTCCTGCTCA	492	0.1207361963190184	No Hit
GGCTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	488	0.11975460122699386	No Hit
GGCTGCGGGAGGCCTTAGTGTCACGCAGGTTTATTACAGTGGCATGATGGTG	486	0.1192638036809816	No Hit
GGCTGCGGGCTCTAACTACAGGAAATGGCAGGTTTTTTTGTGTTTTGTTTTT	471	0.11558282208588956	No Hit
GGCTGCGGGGCTGGCTGCGGGGGAACCTTTTCACGGCCACTCTGCAGGTCTG	449	0.1101840490797546	No Hit
GGCTGCGGGCAGTGCTGGCTGCGGGATATATCAATGCTGTGTGTACGTGCAG	445	0.10920245398773007	No Hit
GGCTGCGGGGTGCAGTGCTGGCTGCGGGATCTCAGAATAACTATGGATTTGA	444	0.10895705521472392	No Hit
GGCTGCGGGATTGTGTGAACATTTTGTTCCTTAGTTTCTTGTTCTTATGAGA	443	0.10871165644171779	No Hit
GGCTGCGGGGTTCTGGTTCACAGTCATCACCGTCTCCTTCGGCTACTACACG	442	0.10846625766871165	No Hit
GGCTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	441	0.10822085889570553	No Hit
GGCTGCGGGGCTGGCTGCGGGGCTGGCTGCGGGGTTCTTTCTCTTTTCTATA	440	0.10797546012269937	No Hit
GGCTGCGGGACCATCGCCTTCCCTTTTCTCCTGCACCCCTGTAGTCTACCTC	440	0.10797546012269937	No Hit
GGCTGCGGGGCTGCGGGAAACTCCAGGACTCTTCTACCCAGCTCCTGCCTGC	433	0.10625766871165644	No Hit
GGCTGCGGGGACAAGGCAACCACAGAAGTCAGAACAATCAGGCTTGAGTGGG	423	0.10380368098159508	No Hit
GGCTGCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGA	421	0.10331288343558283	No Hit
GGCTGCGGGGCTGGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	418	0.10257668711656442	No Hit
GGCTGCGGGATCAGCAGGCTGAAGGAGCAGCTGAAAGCAGCCACAGAGGCAC	410	0.10061349693251534	No Hit
GGCTGCGGGGCTGGCTGCGGGGTCTTTGCTTCTGTCGCTCATCGCAGACACC	409	0.1003680981595092	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTAA	20	6.306635E-4	46.000004	38
CGTGGCA	20	6.306635E-4	46.000004	14
AGCAACT	40	5.5952114E-9	46.000004	37
CTGTCGT	20	6.306635E-4	46.000004	27
TTGGCGC	20	6.306635E-4	46.000004	19
CCTTACT	20	6.306635E-4	46.000004	43
AATCCAG	20	6.306635E-4	46.000004	37
CAAACTA	20	6.306635E-4	46.000004	42
CCGGGTA	20	6.306635E-4	46.000004	40
CGAACGG	20	6.306635E-4	46.000004	11
CGAACCA	20	6.306635E-4	46.000004	46
GCGACGC	20	6.306635E-4	46.000004	29
GGTAATG	20	6.306635E-4	46.000004	19
CACGGTG	40	5.5952114E-9	46.000004	10
CACGGTC	20	6.306635E-4	46.000004	17
CACGGTA	40	5.5952114E-9	46.000004	39
GATCGAG	20	6.306635E-4	46.000004	13
CGAGTCA	20	6.306635E-4	46.000004	27
CGTGCTA	20	6.306635E-4	46.000004	20
TAGACTT	20	6.306635E-4	46.000004	27

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content