##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527205_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	407500
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46549202453988	34.0	31.0	34.0	31.0	34.0
2	32.86201963190184	34.0	31.0	34.0	31.0	34.0
3	32.89358282208589	34.0	31.0	34.0	31.0	34.0
4	36.32232147239264	37.0	37.0	37.0	35.0	37.0
5	36.22568588957055	37.0	37.0	37.0	35.0	37.0
6	36.1083754601227	37.0	37.0	37.0	35.0	37.0
7	36.452306748466256	37.0	37.0	37.0	35.0	37.0
8	36.61654233128834	37.0	37.0	37.0	35.0	37.0
9	38.61733742331288	39.0	39.0	39.0	38.0	39.0
10	37.94047852760736	39.0	38.0	39.0	35.0	39.0
11	37.46079018404908	39.0	37.0	39.0	35.0	39.0
12	37.047671165644175	39.0	37.0	39.0	33.0	39.0
13	36.949693251533745	39.0	37.0	39.0	33.0	39.0
14	37.78373251533743	40.0	38.0	40.0	33.0	40.0
15	37.83679754601227	40.0	38.0	40.0	33.0	40.0
16	37.878554601226995	40.0	38.0	40.0	33.0	40.0
17	37.88508957055215	40.0	38.0	40.0	33.0	40.0
18	37.749555828220856	40.0	37.0	40.0	33.0	40.0
19	37.64665030674847	40.0	37.0	40.0	33.0	40.0
20	37.73536932515337	40.0	37.0	40.0	33.0	40.0
21	33.285811042944786	39.0	33.0	40.0	10.0	40.0
22	33.54655950920245	38.0	32.0	40.0	15.0	40.0
23	35.346954601227	38.0	33.0	40.0	26.0	40.0
24	36.181838036809815	38.0	35.0	40.0	30.0	40.0
25	36.57024785276074	38.0	35.0	40.0	31.0	40.0
26	36.61281717791411	38.0	35.0	40.0	31.0	40.0
27	36.56668220858896	38.0	35.0	40.0	31.0	40.0
28	36.35916319018405	38.0	35.0	40.0	31.0	40.0
29	36.30013742331288	38.0	35.0	40.0	31.0	40.0
30	36.07518036809816	38.0	35.0	40.0	31.0	40.0
31	35.93649325153374	37.0	34.0	40.0	31.0	40.0
32	35.88614478527607	37.0	34.0	40.0	31.0	40.0
33	35.43299141104294	37.0	34.0	40.0	30.0	40.0
34	35.330905521472395	37.0	34.0	40.0	29.0	40.0
35	35.173968098159506	36.0	34.0	40.0	29.0	40.0
36	35.09306257668712	36.0	34.0	40.0	29.0	40.0
37	34.98646871165644	36.0	34.0	39.0	29.0	40.0
38	34.91121717791411	36.0	34.0	39.0	29.0	40.0
39	34.68554846625767	35.0	33.0	39.0	28.0	40.0
40	29.959469938650308	35.0	27.0	38.0	8.0	40.0
41	30.722630674846627	35.0	27.0	38.0	12.0	40.0
42	32.29351411042945	35.0	29.0	38.0	21.0	40.0
43	33.10652024539877	35.0	31.0	38.0	24.0	40.0
44	34.07062576687117	35.0	33.0	39.0	27.0	40.0
45	34.398505521472394	35.0	33.0	39.0	28.0	40.0
46	34.46345030674846	35.0	34.0	39.0	28.0	40.0
47	34.36409079754601	35.0	34.0	39.0	28.0	40.0
48	34.297055214723926	35.0	34.0	39.0	28.0	40.0
49	34.27200981595092	35.0	34.0	39.0	28.0	40.0
50	34.12333006134969	35.0	33.0	38.0	28.0	40.0
51	33.997779141104296	35.0	33.0	38.0	27.0	40.0
52	33.58506503067485	35.0	33.0	37.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	2.0
16	8.0
17	47.0
18	80.0
19	158.0
20	284.0
21	466.0
22	863.0
23	1208.0
24	1739.0
25	2470.0
26	3259.0
27	4503.0
28	5890.0
29	7996.0
30	10789.0
31	14844.0
32	21354.0
33	27358.0
34	40463.0
35	56441.0
36	59158.0
37	50648.0
38	78938.0
39	18531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.06036809815951	0.4645398773006135	0.28883435582822087	0.18625766871165644
8	99.1764417177914	0.38993865030674846	0.3229447852760736	0.11067484662576686
9	96.69496932515338	1.3423312883435583	1.0429447852760736	0.9197546012269939
10	51.346748466257665	33.67680981595092	3.964662576687117	11.011779141104295
11	26.631656441717794	18.029693251533743	28.51239263803681	26.826257668711655
12	27.442699386503065	13.079754601226995	35.900613496932515	23.576932515337422
13	35.0039263803681	15.165398773006137	28.505766871165644	21.32490797546012
14	34.52343558282209	17.808098159509203	25.120981595092022	22.547484662576686
15	23.80957055214724	17.64957055214724	28.488834355828217	30.052024539877305
16	29.182331288343562	18.25398773006135	33.97570552147239	18.5879754601227
17	38.23092024539878	17.998282208588957	21.452269938650307	22.31852760736196
18	26.91116564417178	19.867239263803683	22.476319018404908	30.74527607361963
19	35.890061349693255	20.566625766871166	22.192638036809818	21.35067484662577
20	34.23312883435583	21.722699386503066	24.177914110429448	19.866257668711658
21	39.59263803680982	19.98233128834356	23.976687116564417	16.448343558282208
22	28.25570552147239	22.717055214723928	27.124662576687115	21.902576687116564
23	27.175460122699384	22.648588957055217	28.59558282208589	21.580368098159507
24	26.441963190184048	23.792638036809816	28.2039263803681	21.561472392638038
25	28.841226993865032	24.613251533742332	25.554601226993867	20.990920245398772
26	25.14576687116564	25.074355828220856	26.843435582822085	22.93644171779141
27	24.064785276073618	23.70208588957055	28.335460122699384	23.897668711656443
28	26.251779141104294	24.148957055214723	26.7921472392638	22.80711656441718
29	25.82282208588957	22.67950920245399	26.580368098159507	24.91730061349693
30	24.43361963190184	22.96490797546012	28.786257668711656	23.81521472392638
31	26.56564417177914	22.249815950920247	28.632147239263805	22.55239263803681
32	27.89030674846626	22.639754601226993	28.159754601226993	21.310184049079755
33	27.012760736196316	24.0120245398773	28.700368098159508	20.27484662576687
34	24.269202453987727	24.6920245398773	31.040736196319017	19.99803680981595
35	25.55877300613497	26.065276073619632	30.345030674846623	18.030920245398775
36	24.247361963190183	27.19361963190184	25.801472392638036	22.757546012269938
37	25.47042944785276	25.908220858895703	27.20196319018405	21.419386503067482
38	25.03680981595092	26.09325153374233	26.867239263803683	22.002699386503068
39	26.121472392638033	26.519018404907975	24.66233128834356	22.69717791411043
40	26.117055214723926	28.860613496932512	24.15558282208589	20.86674846625767
41	23.750920245398774	24.97276073619632	26.467484662576684	24.80883435582822
42	22.935214723926382	24.78723926380368	29.19361963190184	23.083926380368098
43	23.531779141104295	24.1079754601227	28.257423312883432	24.10282208588957
44	24.727361963190184	24.21521472392638	27.691288343558284	23.366134969325152
45	24.215950920245398	25.59042944785276	27.09546012269939	23.098159509202453
46	24.165889570552146	23.500122699386502	28.42478527607362	23.909202453987728
47	23.5479754601227	26.142576687116563	27.71926380368098	22.590184049079753
48	22.644907975460125	26.82871165644172	25.924907975460123	24.601472392638037
49	23.256687116564418	25.06993865030675	27.206625766871166	24.466748466257666
50	21.930797546012272	25.268711656441717	28.486871165644175	24.31361963190184
51	23.98233128834356	27.83042944785276	25.233128834355828	22.95411042944785
52	25.45766871165644	24.806625766871164	27.048588957055212	22.687116564417177
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	34.0
12	68.0
13	36.0
14	113.0
15	222.0
16	600.0
17	978.0
18	1252.5
19	1527.0
20	1542.0
21	1557.0
22	1998.5
23	2440.0
24	2330.5
25	2221.0
26	2159.5
27	2098.0
28	2604.0
29	3110.0
30	2715.5
31	2321.0
32	2638.0
33	2955.0
34	4419.5
35	5884.0
36	6359.5
37	6835.0
38	7629.5
39	8651.5
40	8879.0
41	9781.5
42	10684.0
43	11311.5
44	11939.0
45	12613.5
46	13288.0
47	14048.5
48	14809.0
49	15482.0
50	16155.0
51	16938.5
52	17722.0
53	19978.5
54	22235.0
55	22479.5
56	22724.0
57	24388.0
58	26052.0
59	26702.5
60	27353.0
61	28887.5
62	30422.0
63	30254.0
64	27934.0
65	25782.0
66	24911.0
67	24040.0
68	21612.0
69	19184.0
70	17500.0
71	15816.0
72	13465.0
73	11114.0
74	9609.5
75	8105.0
76	6063.0
77	4021.0
78	3902.0
79	3783.0
80	2793.5
81	1804.0
82	1116.5
83	429.0
84	393.0
85	357.0
86	209.5
87	62.0
88	33.5
89	3.0
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	407500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.026503067484661
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.20178661832672	10.248343558282208
2	11.954338346969772	3.5926380368098156
3	5.5133669753237635	2.485398773006135
4	3.009814969052635	1.809079754601227
5	1.874806068623128	1.4085889570552148
6	1.1578723890712523	1.0439263803680983
7	0.8067545277873043	0.8485889570552148
8	0.5732203223751899	0.689079754601227
9	0.4099096892198651	0.5543558282208588
>10	3.2727450884327083	11.373496932515337
>50	1.6265739062270343	17.90110429447853
>100	1.5563503339702447	42.10159509202454
>500	0.03266212663106495	3.3276073619631905
>1k	0.009798637989319485	2.616196319018405
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2185	0.5361963190184049	No Hit
GGCTGCGGGGTCCATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAG	2109	0.5175460122699387	No Hit
GGCTGCGGGACTTCTCAAAATAACAAATCAGGATGTCAAAACATTTGTATAG	1947	0.4777914110429448	No Hit
GGCTGCGGGGGTGAGTTCCCTTCTGTGTCTATGTGTGTGTTAGAGTGCTCAT	1900	0.4662576687116564	No Hit
GGCTGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1273	0.31239263803680983	No Hit
GGCTGCGGGAGGATATGTATTGTTTTTTTTCTCCTATAAAATCCACCTATTT	1247	0.30601226993865027	No Hit
GGCTGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	905	0.22208588957055214	No Hit
GGCTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	884	0.2169325153374233	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	848	0.20809815950920246	No Hit
GGCTGCGGTTGGAGACGGTGGGATGGGAGAAGAGAAGAGTGCGCTGTCCGAG	840	0.2061349693251534	No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	824	0.2022085889570552	No Hit
GGCTGCGGGATTCTCCTGCTTTTTTACCACTGACTAGGAACTGGGTTTGGCC	742	0.18208588957055216	No Hit
GGCTGCGGGATTGAAGAGGGGTCTTCAGGCTCTTTCCCATCACTCTTGCTGC	715	0.17546012269938652	No Hit
GGCTGCGGGACGGGGGGCTGGGGACTCTCTCTTGCAGTCTGGTCTTACCAAG	711	0.17447852760736196	No Hit
GGCTGCGGGATCTTGTTATATACACAGACACTTTTATGGTCGTTTTGTATTG	689	0.16907975460122698	No Hit
GGCTGCGGGGTGGGTTCGAACAAAGGCGCCATCATCGGACTCATGGTGGGCG	667	0.16368098159509203	No Hit
GGCTGCGGGAGAAATAAAAATTTTTAATTAAAATGCTGGCTTTTGCTGTATG	666	0.1634355828220859	No Hit
GGCTGCGGGATCCTTAGGATGTAACTTTGAGAGTCCCTTCATGGTTCTGCAC	663	0.16269938650306748	No Hit
GGCTGCGGGGCTGGCTGCGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCA	631	0.15484662576687117	No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	595	0.1460122699386503	No Hit
GGCTGCGGGTGCAGTGCTGGCTGCGGGTCGTGTATAAAATCAAACTGAAGGG	562	0.13791411042944787	No Hit
GGCTGCGGGATCTGTCCACTTTGCGATCCCAATTCTGTCCCAATCTCACCAG	536	0.13153374233128834	No Hit
GGCTGCGGGGATACCACACCCAATGAACTTGTTCAGTTAAATAAGTTATTCT	533	0.13079754601226992	No Hit
GGCTGCGGGGCTGGCTGCGGGACAGGGGTGCTTGAGTCTGGCCTCAGTCGCT	532	0.1305521472392638	No Hit
GGCTGCGGGCAGCACAGTGACCTGGACAAGTCTTCTATCCACAGCTACTGAG	514	0.1261349693251534	No Hit
GGCTGCGGGCTGGCTGCGGGGCTTTTGTGACTGGCGTGAACGAGTGCGCAGT	503	0.1234355828220859	No Hit
GGCTGCGGGGATGAAGTGGTTTCTGTCATCTTACATTTCACTGTCCTGCTCA	492	0.1207361963190184	No Hit
GGCTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	488	0.11975460122699386	No Hit
GGCTGCGGGAGGCCTTAGTGTCACGCAGGTTTATTACAGTGGCATGATGGTG	486	0.1192638036809816	No Hit
GGCTGCGGGCTCTAACTACAGGAAATGGCAGGTTTTTTTGTGTTTTGTTTTT	471	0.11558282208588956	No Hit
GGCTGCGGGGCTGGCTGCGGGGGAACCTTTTCACGGCCACTCTGCAGGTCTG	449	0.1101840490797546	No Hit
GGCTGCGGGCAGTGCTGGCTGCGGGATATATCAATGCTGTGTGTACGTGCAG	445	0.10920245398773007	No Hit
GGCTGCGGGGTGCAGTGCTGGCTGCGGGATCTCAGAATAACTATGGATTTGA	444	0.10895705521472392	No Hit
GGCTGCGGGATTGTGTGAACATTTTGTTCCTTAGTTTCTTGTTCTTATGAGA	443	0.10871165644171779	No Hit
GGCTGCGGGGTTCTGGTTCACAGTCATCACCGTCTCCTTCGGCTACTACACG	442	0.10846625766871165	No Hit
GGCTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	441	0.10822085889570553	No Hit
GGCTGCGGGGCTGGCTGCGGGGCTGGCTGCGGGGTTCTTTCTCTTTTCTATA	440	0.10797546012269937	No Hit
GGCTGCGGGACCATCGCCTTCCCTTTTCTCCTGCACCCCTGTAGTCTACCTC	440	0.10797546012269937	No Hit
GGCTGCGGGGCTGCGGGAAACTCCAGGACTCTTCTACCCAGCTCCTGCCTGC	433	0.10625766871165644	No Hit
GGCTGCGGGGACAAGGCAACCACAGAAGTCAGAACAATCAGGCTTGAGTGGG	423	0.10380368098159508	No Hit
GGCTGCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGA	421	0.10331288343558283	No Hit
GGCTGCGGGGCTGGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	418	0.10257668711656442	No Hit
GGCTGCGGGATCAGCAGGCTGAAGGAGCAGCTGAAAGCAGCCACAGAGGCAC	410	0.10061349693251534	No Hit
GGCTGCGGGGCTGGCTGCGGGGTCTTTGCTTCTGTCGCTCATCGCAGACACC	409	0.1003680981595092	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.45398773006135E-4	0.0	0.0	0.0	0.0
9	2.45398773006135E-4	0.0	0.0	0.0	0.0
10	0.0026993865030674846	0.0	0.0	0.0	0.0
11	0.0049079754601227	0.0	0.0	0.0	0.0
12	0.0098159509202454	0.0	0.0	0.0	0.0
13	0.016932515337423314	0.0	0.0	0.0	0.0
14	0.022085889570552148	0.0	0.0	0.0	0.0
15	0.030429447852760735	0.0	0.0	0.0	0.0
16	0.04760736196319018	0.0	0.0	0.0	0.0
17	0.0547239263803681	0.0	0.0	0.0	0.0
18	0.06404907975460122	0.0	0.0	0.0	0.0
19	0.07263803680981595	0.0	0.0	0.0	0.0
20	0.07901840490797546	0.0	0.0	0.0	0.0
21	0.08736196319018405	0.0	0.0	0.0	0.0
22	0.09987730061349694	0.0	0.0	0.0	0.0
23	0.10503067484662576	0.0	0.0	0.0	0.0
24	0.1158282208588957	0.0	0.0	0.0	0.0
25	0.12220858895705522	0.0	0.0	0.0	0.0
26	0.12932515337423311	0.0	0.0	0.0	0.0
27	0.13865030674846626	0.0	0.0	0.0	0.0
28	0.1501840490797546	0.0	0.0	0.0	0.0
29	0.15558282208588958	0.0	0.0	0.0	0.0
30	0.16466257668711656	0.0	0.0	0.0	0.0
31	0.1705521472392638	0.0	0.0	0.0	0.0
32	0.17693251533742332	0.0	0.0	0.0	0.0
33	0.18404907975460122	0.0	0.0	0.0	0.0
34	0.190920245398773	0.0	0.0	0.0	0.0
35	0.20122699386503068	0.0	0.0	0.0	0.0
36	0.21374233128834355	0.0	0.0	0.0	0.0
37	0.22478527607361964	0.0	0.0	0.0	0.0
38	0.23190184049079754	0.0	0.0	0.0	0.0
39	0.24245398773006135	0.0	0.0	0.0	0.0
40	0.2517791411042945	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTAA	20	6.306635E-4	46.000004	38
CGTGGCA	20	6.306635E-4	46.000004	14
AGCAACT	40	5.5952114E-9	46.000004	37
CTGTCGT	20	6.306635E-4	46.000004	27
TTGGCGC	20	6.306635E-4	46.000004	19
CCTTACT	20	6.306635E-4	46.000004	43
AATCCAG	20	6.306635E-4	46.000004	37
CAAACTA	20	6.306635E-4	46.000004	42
CCGGGTA	20	6.306635E-4	46.000004	40
CGAACGG	20	6.306635E-4	46.000004	11
CGAACCA	20	6.306635E-4	46.000004	46
GCGACGC	20	6.306635E-4	46.000004	29
GGTAATG	20	6.306635E-4	46.000004	19
CACGGTG	40	5.5952114E-9	46.000004	10
CACGGTC	20	6.306635E-4	46.000004	17
CACGGTA	40	5.5952114E-9	46.000004	39
GATCGAG	20	6.306635E-4	46.000004	13
CGAGTCA	20	6.306635E-4	46.000004	27
CGTGCTA	20	6.306635E-4	46.000004	20
TAGACTT	20	6.306635E-4	46.000004	27
>>END_MODULE