FastQCFastQC Report
Fri 17 Jun 2016
SRR1527204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028464
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC39300.3821232439832605No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT30080.29247499183248027No Hit
TAGCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC28680.27886245896793665No Hit
TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA24370.23695530422066305No Hit
TAGCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG21550.209535773736368No Hit
TAGCATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC19650.19106162199163024No Hit
TAGCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC18530.18017159569999533No Hit
TAGCATGGGATTCTATAAGGTCTGTTCTCTAAAGAACCTTAACTAATGCA17960.174629350176574No Hit
TAGCATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT16480.1602389582911993No Hit
TAGCATGGGACCACTCCCTGGCCAGGTCATCCATCACTGTCAGTCACTGT15150.14730705206988287No Hit
TAGCATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT14410.1401118561271955No Hit
TAGCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC13960.13573639913502078No Hit
TAGCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG13540.13165263927565768No Hit
TAGCATGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC13400.1302913859892033No Hit
TAGCATGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTG12920.12562423186421695No Hit
TAGCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC12160.11823457116632181No Hit
TAGCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA11040.10734454487468692No Hit
TAGCATGGAAAAGAGGATGGGTAAAGGTGAGCAACAGGGGAATGGCTGGG10960.10656668585385584No Hit
TAGCATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT10900.10598329158823254No Hit
TAGCATGGGAAGCAGTGACTCTCCATTCTTCCTCCCTTTGGACATATAAC10660.10364971452573934No Hit
TAGCATGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCA10650.10355248214813546No Hit
TAGCATGGGTGCTTCCCTGTTCTTGAAGAGTACTTGAATGTCGGGGTCTG10430.10141336984085005No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGCATGGGA10390.10102444033043452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG207.856094E-444.00000410
GTATTTC207.856094E-444.00000437
CGACCAA408.310963E-944.00000431
GTCTAAT207.856094E-444.00000440
TAAGTAG207.856094E-444.00000413
CGATTCG207.856094E-444.00000427
CGATTCC207.856094E-444.00000432
CTAAGCG207.856094E-444.00000435
TTATCGA207.856094E-444.00000426
ATCGTTT207.856094E-444.00000434
TTATATC207.856094E-444.00000415
CATTAGA207.856094E-444.00000439
ACGATAC650.044.00000435
TACGCGC207.856094E-444.00000443
CTACGTT207.856094E-444.00000440
CGTAGCG207.856094E-444.00000412
TTAAGCG207.856094E-444.00000412
TACGACG408.310963E-944.00000416
AACGCGT207.856094E-444.00000416
ATCCAAC207.856094E-444.00000417
Produced by FastQC (version 0.11.5)