##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527204_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1028464
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70955522021189	34.0	31.0	34.0	31.0	34.0
2	32.98894176169511	34.0	31.0	34.0	31.0	34.0
3	33.077373636802065	34.0	33.0	34.0	31.0	34.0
4	36.36983501610168	37.0	37.0	37.0	35.0	37.0
5	36.12332954775277	37.0	37.0	37.0	35.0	37.0
6	36.29072675368316	37.0	37.0	37.0	35.0	37.0
7	36.619726115838766	37.0	37.0	37.0	35.0	37.0
8	36.71805138536691	37.0	37.0	37.0	35.0	37.0
9	38.77046936013317	39.0	39.0	39.0	39.0	39.0
10	38.27971129762442	39.0	39.0	39.0	37.0	39.0
11	38.07652188117426	39.0	38.0	39.0	37.0	39.0
12	37.731815600740525	39.0	37.0	39.0	35.0	39.0
13	37.58324161079046	39.0	37.0	39.0	35.0	39.0
14	38.476180984458374	40.0	38.0	40.0	35.0	40.0
15	38.491364792545	40.0	38.0	40.0	35.0	40.0
16	38.33805850277696	40.0	38.0	40.0	35.0	40.0
17	38.44129303505033	40.0	38.0	40.0	35.0	40.0
18	38.408382792202744	40.0	38.0	40.0	35.0	40.0
19	38.39863816331928	40.0	38.0	40.0	35.0	40.0
20	38.13939622582803	40.0	38.0	40.0	34.0	40.0
21	38.35440521009972	40.0	38.0	40.0	35.0	40.0
22	38.16293132282705	40.0	38.0	40.0	34.0	40.0
23	38.21606590021624	40.0	38.0	40.0	34.0	40.0
24	38.26545703106769	40.0	38.0	40.0	35.0	40.0
25	38.22395727998258	40.0	38.0	40.0	35.0	40.0
26	38.10958769582601	40.0	38.0	40.0	34.0	40.0
27	37.98422112976244	40.0	38.0	40.0	34.0	40.0
28	37.89614512515752	40.0	38.0	40.0	34.0	40.0
29	38.079574977831015	40.0	38.0	40.0	34.0	40.0
30	38.10900041226528	40.0	38.0	40.0	35.0	40.0
31	38.07179152600383	40.0	38.0	40.0	34.0	40.0
32	37.35068315468504	40.0	37.0	40.0	33.0	40.0
33	37.5886856516125	40.0	37.0	40.0	33.0	40.0
34	37.651076751349585	40.0	37.0	40.0	33.0	40.0
35	37.62154825059506	40.0	37.0	40.0	33.0	40.0
36	37.43057024844817	40.0	37.0	40.0	33.0	40.0
37	37.363182376826025	40.0	36.0	40.0	33.0	40.0
38	37.18780628198945	40.0	36.0	40.0	33.0	40.0
39	37.15503216447051	40.0	35.0	40.0	33.0	40.0
40	37.030697233933324	40.0	35.0	40.0	32.0	40.0
41	36.90671914622194	40.0	35.0	40.0	32.0	40.0
42	36.809805690816596	40.0	35.0	40.0	32.0	40.0
43	36.67284513604754	39.0	35.0	40.0	31.0	40.0
44	36.5648860825464	39.0	35.0	40.0	31.0	40.0
45	36.49160398419391	39.0	35.0	40.0	31.0	40.0
46	36.17615978780006	39.0	35.0	40.0	31.0	40.0
47	36.14893180509964	39.0	35.0	40.0	30.0	40.0
48	36.061877712783335	38.0	35.0	40.0	30.0	40.0
49	35.75794874686912	38.0	35.0	40.0	30.0	40.0
50	35.30992625896482	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	3.0
15	7.0
16	21.0
17	41.0
18	108.0
19	192.0
20	414.0
21	585.0
22	964.0
23	1453.0
24	2077.0
25	2794.0
26	3632.0
27	5257.0
28	6956.0
29	9455.0
30	12452.0
31	16460.0
32	20829.0
33	27449.0
34	45363.0
35	62042.0
36	83682.0
37	160101.0
38	320411.0
39	245714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.3113030694317	0.6264682089018186	0.032086684609281414	0.030142037057203752
8	99.51354641484778	0.4180019913190934	0.03782339488791051	0.030628198945223166
9	97.65212977799904	1.6398240482894881	0.30589305994181615	0.4021531137696604
10	46.558848924220975	41.2861315515176	4.088427013488075	8.066592510773347
11	31.963782883990106	26.667729740661798	22.133297811104715	19.235189564243377
12	29.47288383453383	17.29861229950684	27.369941971717044	25.85856189424229
13	23.773705253659827	18.78082266370043	30.008148073243206	27.437324009396534
14	23.300669736616936	19.90580127257736	29.74134242909815	27.052186561707558
15	24.695370960967033	19.695098710309743	27.994757230199596	27.614773098523628
16	27.696837225221298	21.49623127304407	24.684092005164985	26.122839496569643
17	30.11967361035486	20.60392974377324	23.05262994134943	26.223766704522472
18	27.554197327276402	20.41666018450816	24.322095863345726	27.707046624869708
19	29.264806546461518	19.93010936697833	23.177087384682398	27.62799670187775
20	26.579928903685495	20.96573142083729	25.3931105026525	27.061229172824717
21	28.820746277944586	19.526886697055026	25.760162728107154	25.89220429689323
22	29.150558502776956	18.599484279469188	25.50852533486831	26.741431882885546
23	26.49815647412063	21.970822508128627	24.552244901134117	26.978776116616626
24	26.94766175578338	22.10335023880272	24.176344529317507	26.77264347609639
25	27.729312839341	23.24826148508844	23.177962476080836	25.844463199489727
26	28.070501252353026	23.39936059988488	22.81752205230324	25.712616095458856
27	27.145237947074474	20.303870626487655	23.826794131831548	28.724097294606327
28	28.307651021328894	21.142694348076354	23.08743913253162	27.46221549806313
29	28.906116305480793	21.33433936433361	22.546243718788407	27.21330061139719
30	27.16001726847026	21.054504581589633	24.802812738219327	26.98266541172078
31	29.479009474322876	22.26961760450536	21.572655921840724	26.678716999331044
32	29.101456152086996	23.0819941193858	22.49704413572084	25.319505592806358
33	29.39509793245072	22.288091756250097	22.26008883150018	26.056721479799
34	26.854415905661256	24.763433625289753	24.547577747009132	23.834572722039855
35	28.847971343673674	23.706128751225126	24.04673376997153	23.399166135129672
36	29.430393285520935	24.206194869241898	21.465019679833226	24.898392165403944
37	29.31138085533378	24.401340251092893	21.488452682835764	24.798826210737566
38	29.984617837863066	23.449143577218067	21.95886292568335	24.60737565923552
39	28.013912008587567	23.64710869801957	22.564134476267522	25.77484481712534
40	27.760621664929445	24.29263445293175	23.797429953795174	24.149313928343627
41	26.83944211951026	24.008618677950807	23.144125608674685	26.00781359386425
42	27.03799063457739	22.664867219465144	23.710601440594907	26.58654070536256
43	27.097691314426175	22.080695094821014	24.801257020177665	26.02035657057515
44	25.09295415298931	22.966190357659578	26.062555422455237	25.878300066895875
45	27.092926927923582	22.055998070909627	24.776754461021486	26.0743205401453
46	26.655089531573296	23.86490922385227	24.15689805379673	25.3231031907777
47	26.351043886805954	23.110774903156553	24.469111218282798	26.069069991754695
48	27.750315032903437	23.23542681124473	23.688237993746014	25.326020162105824
49	26.595486084102117	23.481619191337764	23.4675204965852	26.45537422797492
50	25.165392274304203	24.65570015090465	23.96700322033635	26.211904354454802
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	2.5
6	4.0
7	42.0
8	80.0
9	573.5
10	1067.0
11	1415.0
12	1763.0
13	2355.5
14	2948.0
15	2343.0
16	1738.0
17	2300.5
18	2863.0
19	2952.5
20	3042.0
21	3199.0
22	3356.0
23	3355.5
24	3355.0
25	3863.0
26	4371.0
27	6055.5
28	7740.0
29	9199.5
30	10659.0
31	11474.0
32	12289.0
33	13223.0
34	14157.0
35	17342.0
36	20527.0
37	22857.0
38	25187.0
39	24873.0
40	24559.0
41	26257.0
42	27955.0
43	32795.5
44	37636.0
45	39960.0
46	42284.0
47	44720.0
48	47156.0
49	50695.0
50	54234.0
51	58491.5
52	62749.0
53	63368.5
54	63988.0
55	75605.5
56	87223.0
57	87908.5
58	88594.0
59	89075.0
60	89556.0
61	81511.5
62	73467.0
63	70995.5
64	68524.0
65	57107.0
66	45690.0
67	37508.5
68	29327.0
69	26586.5
70	23846.0
71	20237.0
72	16628.0
73	16954.0
74	17280.0
75	13020.5
76	8761.0
77	5654.0
78	2547.0
79	1741.0
80	935.0
81	601.5
82	268.0
83	177.0
84	86.0
85	51.0
86	16.0
87	11.0
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1028464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.808577594488202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.83315201292616	6.849638901864763
2	11.769174897876725	2.308777304178532
3	4.668389270390197	1.3737077519969525
4	2.2615577429721885	0.8873065842543328
5	1.2027616255998588	0.5898690366184492
6	0.7318548757438479	0.4307073201982833
7	0.4679072135969071	0.32126529481102206
8	0.3139377458190637	0.24634261917640005
9	0.20795876156016863	0.18358016842949262
>10	2.9734103696141934	7.981912739838201
>50	2.6824680637776983	19.435569669173674
>100	2.746455375026961	47.68151642802095
>500	0.11497719990101951	7.6146543366500365
>1k	0.025994845195013105	4.095151844788904
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAGCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3930	0.3821232439832605	No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3008	0.29247499183248027	No Hit
TAGCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2868	0.27886245896793665	No Hit
TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2437	0.23695530422066305	No Hit
TAGCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2155	0.209535773736368	No Hit
TAGCATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1965	0.19106162199163024	No Hit
TAGCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1853	0.18017159569999533	No Hit
TAGCATGGGATTCTATAAGGTCTGTTCTCTAAAGAACCTTAACTAATGCA	1796	0.174629350176574	No Hit
TAGCATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	1648	0.1602389582911993	No Hit
TAGCATGGGACCACTCCCTGGCCAGGTCATCCATCACTGTCAGTCACTGT	1515	0.14730705206988287	No Hit
TAGCATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	1441	0.1401118561271955	No Hit
TAGCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	1396	0.13573639913502078	No Hit
TAGCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1354	0.13165263927565768	No Hit
TAGCATGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC	1340	0.1302913859892033	No Hit
TAGCATGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTG	1292	0.12562423186421695	No Hit
TAGCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	1216	0.11823457116632181	No Hit
TAGCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1104	0.10734454487468692	No Hit
TAGCATGGAAAAGAGGATGGGTAAAGGTGAGCAACAGGGGAATGGCTGGG	1096	0.10656668585385584	No Hit
TAGCATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1090	0.10598329158823254	No Hit
TAGCATGGGAAGCAGTGACTCTCCATTCTTCCTCCCTTTGGACATATAAC	1066	0.10364971452573934	No Hit
TAGCATGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCA	1065	0.10355248214813546	No Hit
TAGCATGGGTGCTTCCCTGTTCTTGAAGAGTACTTGAATGTCGGGGTCTG	1043	0.10141336984085005	No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGCATGGGA	1039	0.10102444033043452	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.9446475520776616E-4	0.0	0.0	0.0	0.0
8	4.8616188801941536E-4	0.0	0.0	0.0	0.0
9	4.8616188801941536E-4	0.0	0.0	0.0	0.0
10	0.007681357830706763	0.0	0.0	0.0	0.0
11	0.01516825090620576	0.0	0.0	0.0	0.0
12	0.018863081255153315	0.0	0.0	0.0	0.0
13	0.02275237635930864	0.0	0.0	0.0	0.0
14	0.030822663700430936	0.0	0.0	0.0	0.0
15	0.0379206272655144	0.0	0.0	0.0	0.0
16	0.04851895642433765	0.0	0.0	0.0	0.0
17	0.054547363835778406	0.0	0.0	0.0	0.0
18	0.05795049705191431	0.0	0.0	0.0	0.0
19	0.06456229872897835	0.0	0.0	0.0	0.0
20	0.07029900900760747	0.0	0.0	0.0	0.0
21	0.07749420495029481	0.0	0.0	0.0	0.0
22	0.08332814760652779	0.0	0.0	0.0	0.0
23	0.0908150406820268	0.0	0.0	9.723237760388308E-5	0.0
24	0.0970379128486753	0.0	0.0	9.723237760388308E-5	0.0
25	0.10258015837209664	0.0	0.0	9.723237760388308E-5	0.0
26	0.10763624200749856	0.0	0.0	9.723237760388308E-5	0.0
27	0.1136646494189393	0.0	0.0	9.723237760388308E-5	0.0
28	0.12562423186421692	0.0	0.0	9.723237760388308E-5	0.0
29	0.1332083573173198	0.0	0.0	9.723237760388308E-5	0.0
30	0.14273713032250035	0.0	0.0	9.723237760388308E-5	0.0
31	0.15080741766362266	0.0	0.0	9.723237760388308E-5	0.0
32	0.15936386689276436	0.0	0.0	9.723237760388308E-5	0.0
33	0.16626736570264006	0.0	0.0	9.723237760388308E-5	0.0
34	0.174629350176574	0.0	0.0	9.723237760388308E-5	0.0
35	0.19436752283016226	0.0	0.0	9.723237760388308E-5	0.0
36	0.20185441590566125	0.0	0.0	9.723237760388308E-5	0.0
37	0.21109149177803016	0.0	0.0	9.723237760388308E-5	0.0
38	0.2205230324056068	0.0	0.0	9.723237760388308E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.856094E-4	44.000004	10
GTATTTC	20	7.856094E-4	44.000004	37
CGACCAA	40	8.310963E-9	44.000004	31
GTCTAAT	20	7.856094E-4	44.000004	40
TAAGTAG	20	7.856094E-4	44.000004	13
CGATTCG	20	7.856094E-4	44.000004	27
CGATTCC	20	7.856094E-4	44.000004	32
CTAAGCG	20	7.856094E-4	44.000004	35
TTATCGA	20	7.856094E-4	44.000004	26
ATCGTTT	20	7.856094E-4	44.000004	34
TTATATC	20	7.856094E-4	44.000004	15
CATTAGA	20	7.856094E-4	44.000004	39
ACGATAC	65	0.0	44.000004	35
TACGCGC	20	7.856094E-4	44.000004	43
CTACGTT	20	7.856094E-4	44.000004	40
CGTAGCG	20	7.856094E-4	44.000004	12
TTAAGCG	20	7.856094E-4	44.000004	12
TACGACG	40	8.310963E-9	44.000004	16
AACGCGT	20	7.856094E-4	44.000004	16
ATCCAAC	20	7.856094E-4	44.000004	17
>>END_MODULE