FastQCFastQC Report
Fri 17 Jun 2016
SRR1527203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100428
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT34500.3135143780419982No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG29440.2675322692625051No Hit
TAGCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC28410.25817227478762805No Hit
TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC23230.21109968121494543No Hit
TAGCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG21380.1942880406532731No Hit
TAGCATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG19970.18147484433329578No Hit
TAGCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT18480.16793465815119207No Hit
TAGCATGGGATTCTATAAGGTCTGTTCTCTAAAGAACCTTAACTAATGCATC17840.1621187392541811No Hit
TAGCATGGGACCACTCCCTGGCCAGGTCATCCATCACTGTCAGTCACTGTCT15430.14021816965762413No Hit
TAGCATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC15070.13694671527805546No Hit
TAGCATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG13770.12513313001850188No Hit
TAGCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT13450.1222251705699964No Hit
TAGCATGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG13110.11913546365595931No Hit
TAGCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC13080.11886284245766193No Hit
TAGCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA12790.11622750420745383No Hit
TAGCATGGGGTTCTCAATGTTATTCTGTAGTAAATGGCACTATTATGAAGCC12140.11032071157767705No Hit
TAGCATGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT11910.10823061572406373No Hit
TAGCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG11330.10295993922364753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGC351.0195254E-746.00000427
CCGTATT351.0195254E-746.00000445
TGTTACG351.0195254E-746.00000426
ATCGCTA351.0195254E-746.00000443
TCACGAA405.6115823E-946.011
AACCGAT600.046.040
ACGCATT253.4170604E-546.022
CGACGAA206.311337E-446.020
GCGCGTA650.046.010
GACCGTT206.311337E-446.046
CGGTTAG253.4170604E-546.042
CGACCAA301.86133E-646.031
CGTCTAA501.6370905E-1146.016
CACGATA900.046.034
GTCTAAT206.311337E-446.040
CACGAAT453.092282E-1046.044
TACGGAT206.311337E-446.020
CGTTCAT206.311337E-446.043
CGTTCAC206.311337E-446.029
TTATATC301.86133E-646.015
Produced by FastQC (version 0.11.5)