##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527203_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1100428
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.680851450526525	34.0	31.0	34.0	31.0	34.0
2	32.93463815897087	34.0	31.0	34.0	31.0	34.0
3	33.02284474768	34.0	31.0	34.0	31.0	34.0
4	36.33500692457844	37.0	37.0	37.0	35.0	37.0
5	36.146184030213696	37.0	37.0	37.0	35.0	37.0
6	36.30934963486934	37.0	37.0	37.0	35.0	37.0
7	36.60156320995104	37.0	37.0	37.0	35.0	37.0
8	36.67185676845737	37.0	37.0	37.0	35.0	37.0
9	38.73053575517889	39.0	39.0	39.0	39.0	39.0
10	38.18032801782579	39.0	39.0	39.0	37.0	39.0
11	37.98371906203768	39.0	38.0	39.0	35.0	39.0
12	37.65484702315826	39.0	37.0	39.0	35.0	39.0
13	37.55820189962451	39.0	37.0	39.0	35.0	39.0
14	38.449467843420926	40.0	38.0	40.0	35.0	40.0
15	38.36453089161672	40.0	38.0	40.0	35.0	40.0
16	33.21785069082212	40.0	35.0	40.0	3.0	40.0
17	35.001773855263586	40.0	35.0	40.0	17.0	40.0
18	37.10335614869851	40.0	35.0	40.0	32.0	40.0
19	37.96735270276656	40.0	37.0	40.0	34.0	40.0
20	38.17360699655043	40.0	38.0	40.0	34.0	40.0
21	38.37586284609261	40.0	38.0	40.0	35.0	40.0
22	38.391516755298845	40.0	38.0	40.0	35.0	40.0
23	38.351343295517744	40.0	38.0	40.0	35.0	40.0
24	38.32968535878767	40.0	38.0	40.0	35.0	40.0
25	38.268893557779336	40.0	38.0	40.0	35.0	40.0
26	38.18881108077948	40.0	38.0	40.0	35.0	40.0
27	38.052944854184005	40.0	38.0	40.0	34.0	40.0
28	38.00090782859033	40.0	38.0	40.0	34.0	40.0
29	37.8047450628301	40.0	38.0	40.0	34.0	40.0
30	37.80316113366799	40.0	38.0	40.0	34.0	40.0
31	37.77232949361521	40.0	37.0	40.0	34.0	40.0
32	37.702585721192115	40.0	37.0	40.0	33.0	40.0
33	37.3859525566416	40.0	37.0	40.0	33.0	40.0
34	37.42218936631929	40.0	37.0	40.0	33.0	40.0
35	37.31414413301007	40.0	36.0	40.0	33.0	40.0
36	37.26365559582272	40.0	36.0	40.0	33.0	40.0
37	36.91295205138364	39.0	35.0	40.0	32.0	40.0
38	36.9378260095163	39.0	35.0	40.0	32.0	40.0
39	36.5661987881079	39.0	35.0	40.0	31.0	40.0
40	36.52129807674832	39.0	35.0	40.0	31.0	40.0
41	36.501037778028184	39.0	35.0	40.0	31.0	40.0
42	36.417118612030954	39.0	35.0	40.0	31.0	40.0
43	36.29408739145133	38.0	35.0	40.0	31.0	40.0
44	36.55875441191973	39.0	35.0	40.0	31.0	40.0
45	36.63556089085338	39.0	35.0	40.0	32.0	40.0
46	36.55643622299687	39.0	35.0	40.0	32.0	40.0
47	36.275339231644416	39.0	35.0	40.0	31.0	40.0
48	36.243866931775635	38.0	35.0	40.0	31.0	40.0
49	36.17283911350856	38.0	35.0	40.0	31.0	40.0
50	35.672093948899885	38.0	35.0	40.0	30.0	40.0
51	35.740752688953755	37.0	35.0	40.0	30.0	40.0
52	35.41285299901493	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	5.0
16	13.0
17	31.0
18	99.0
19	186.0
20	306.0
21	545.0
22	932.0
23	1577.0
24	2331.0
25	3199.0
26	4460.0
27	6343.0
28	8468.0
29	11331.0
30	14771.0
31	19099.0
32	25562.0
33	37085.0
34	55750.0
35	79245.0
36	122132.0
37	222103.0
38	290215.0
39	194637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.36406561810496	0.595041202150436	0.024899402777828265	0.0159937769667802
8	99.52091368085873	0.4007531614971629	0.048163078365872185	0.030170079278244465
9	97.6678165222986	1.5792037280040128	0.2918864296437386	0.46109332005365183
10	46.83504963523284	41.06602158432901	4.043790234345182	8.055138546092975
11	32.11777599261379	26.530677154707078	22.020977292471656	19.330569560207483
12	29.443907279712985	17.29954163289193	27.40688168603489	25.8496694013602
13	23.81091720675955	18.83830654981516	29.86010897578033	27.49066726764495
14	23.412345014848768	19.815017429581943	29.695354898275944	27.077282657293345
15	24.71910929202092	19.837735862773393	27.882151308400005	27.56100353680568
16	26.542672487432164	29.208907806780633	21.789067526453344	22.45935217933386
17	30.283126201805118	20.38979378932561	22.879734067108433	26.44734594176084
18	27.770467490830843	20.314641212328294	24.238114624491562	27.676776672349302
19	29.35476014786974	19.901529223174983	23.048304841388987	27.69540578756629
20	26.57665926348657	20.948212877171425	25.36921997622743	27.105907883114572
21	29.102494665711887	19.373734583271236	25.77787915247522	25.74589159854166
22	28.984904055512946	18.4410065901631	25.41674693846394	27.157342415860008
23	26.579112854271248	21.861493891467685	24.555900067973553	27.00349318628752
24	27.123810008469434	21.944098114551792	24.067635501822927	26.86445637515585
25	27.808180089928648	23.014681560265643	23.218511342859323	25.95862700694639
26	28.01673530662615	23.325287978859137	22.935257917828338	25.72271879668638
27	27.16234047116213	20.208137197526778	23.682512622361482	28.94700970894961
28	28.381593343680823	21.049900584136356	22.992144874539726	27.576361197643102
29	29.314684831720022	21.166128088343807	22.37002330002508	27.14916377991109
30	27.31818892285547	21.040631463394245	24.67185495098271	26.96932466276758
31	29.703170039293802	22.059326007698825	21.565427270116718	26.672076682890655
32	29.2998724132792	22.826118564776614	22.449356068729625	25.424652953214565
33	29.858382374857783	22.233258332212557	22.177461860294358	25.730897432635302
34	26.99267921208839	24.630053033910443	24.492833697434087	23.88443405656708
35	29.05642168319963	23.503491368812863	23.9521349874776	23.48795196050991
36	29.444361648376816	24.128611776508777	21.353600599039645	25.073425976074766
37	29.58212622724976	24.16632437560658	21.470373345643694	24.781176051499962
38	29.969066581366523	23.297298869167268	21.88194048133999	24.85169406812622
39	28.40776497871737	23.437698786290426	22.45889781067003	25.69563842432217
40	27.69976772673905	24.218485898214148	23.71795337814014	24.363792996906657
41	26.850916188973745	23.8267292362608	23.33210350881657	25.990251065948883
42	27.162613092360427	22.6117474291821	23.589276172543773	26.6363633059137
43	27.23240411912456	21.986808768951718	24.736466174979192	26.04432093694453
44	25.115954883009156	22.875644749133972	26.068311602394704	25.940088765462164
45	27.0540189817053	21.92792259011948	24.862598916058115	26.1554595121171
46	26.489056985100344	23.692145238034655	24.26601286045066	25.55278491641434
47	26.790666904150022	23.076203077348087	24.271101789485545	25.86202822901635
48	27.963937667889223	23.154172740061142	23.712864449105258	25.169025142944378
49	26.682618035891494	23.25985889126776	23.430701508867458	26.62682156397329
50	25.285343520884602	24.54363211405017	23.834271756080362	26.33675260898487
51	25.299065454532236	24.327352630067573	24.199584161798864	26.173997753601324
52	25.592678485098524	25.40211626748865	22.34148894793662	26.663716299476203
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	2.0
6	1.0
7	43.0
8	85.0
9	592.5
10	1100.0
11	1457.5
12	1815.0
13	2415.5
14	2410.0
15	1804.0
16	2430.0
17	3056.0
18	3015.5
19	2975.0
20	3160.5
21	3346.0
22	3022.5
23	2699.0
24	3426.0
25	4153.0
26	5268.5
27	6384.0
28	7963.0
29	9542.0
30	10221.5
31	10901.0
32	11766.5
33	12632.0
34	14150.0
35	15668.0
36	19258.0
37	22848.0
38	24853.0
39	25795.5
40	24733.0
41	27240.5
42	29748.0
43	35303.0
44	40858.0
45	41566.5
46	42275.0
47	46148.5
48	50022.0
49	52174.5
50	54327.0
51	57858.0
52	61389.0
53	65263.5
54	69138.0
55	76697.0
56	84256.0
57	91871.0
58	99486.0
59	94422.0
60	89358.0
61	86765.0
62	84172.0
63	77039.5
64	60853.5
65	51800.0
66	43875.5
67	35951.0
68	30620.5
69	25290.0
70	24094.0
71	22898.0
72	21748.5
73	20599.0
74	14839.5
75	9080.0
76	6456.5
77	3833.0
78	2801.5
79	1770.0
80	1131.0
81	492.0
82	307.0
83	122.0
84	76.0
85	30.0
86	17.5
87	5.0
88	3.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1100428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.588859571092316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.23013350664762	8.02298295296512
2	10.437223155313834	2.4191102691817017
3	4.306449481164416	1.4972051486165334
4	2.37413173450522	1.1005391709781926
5	1.5118440137903195	0.8760273984606283
6	1.0177723610490652	0.707689258052201
7	0.7523110394942378	0.6102898893336844
8	0.6105449703895128	0.5660415938944624
9	0.504660566401912	0.52635963915897
>10	4.811736136613663	12.823812303442722
>50	2.279703484625732	19.298073573795577
>100	2.064787304161714	41.98405433890672
>500	0.0811905570641846	6.188117137664988
>1k	0.017511688778549618	3.3796973255484977
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAGCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3450	0.3135143780419982	No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2944	0.2675322692625051	No Hit
TAGCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2841	0.25817227478762805	No Hit
TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2323	0.21109968121494543	No Hit
TAGCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2138	0.1942880406532731	No Hit
TAGCATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1997	0.18147484433329578	No Hit
TAGCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1848	0.16793465815119207	No Hit
TAGCATGGGATTCTATAAGGTCTGTTCTCTAAAGAACCTTAACTAATGCATC	1784	0.1621187392541811	No Hit
TAGCATGGGACCACTCCCTGGCCAGGTCATCCATCACTGTCAGTCACTGTCT	1543	0.14021816965762413	No Hit
TAGCATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	1507	0.13694671527805546	No Hit
TAGCATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	1377	0.12513313001850188	No Hit
TAGCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1345	0.1222251705699964	No Hit
TAGCATGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG	1311	0.11913546365595931	No Hit
TAGCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1308	0.11886284245766193	No Hit
TAGCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1279	0.11622750420745383	No Hit
TAGCATGGGGTTCTCAATGTTATTCTGTAGTAAATGGCACTATTATGAAGCC	1214	0.11032071157767705	No Hit
TAGCATGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT	1191	0.10823061572406373	No Hit
TAGCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1133	0.10295993922364753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.8174746553159315E-4	0.0	0.0	0.0	0.0
8	3.634949310631863E-4	0.0	0.0	0.0	0.0
9	5.452423965947795E-4	0.0	0.0	0.0	0.0
10	0.007360772354029523	0.0	0.0	0.0	0.0
11	0.012904070052743114	0.0	0.0	0.0	0.0
12	0.014903292173590639	0.0	0.0	0.0	0.0
13	0.01890173641528569	0.0	0.0	0.0	0.0
14	0.025717266372720433	0.0	0.0	0.0	0.0
15	0.03298716499398416	0.0	0.0	0.0	0.0
16	0.04480075025353771	0.0	0.0	0.0	0.0
17	0.05034404795225131	0.0	0.0	0.0	0.0
18	0.055523850719901714	0.0	0.0	0.0	0.0
19	0.06243025441010225	0.0	0.0	0.0	0.0
20	0.06588345625520252	0.0	0.0	0.0	0.0
21	0.0717902488849793	0.0	0.0	0.0	0.0
22	0.07778791524752188	0.0	0.0	0.0	0.0
23	0.08442169773942503	0.0	0.0	0.0	0.0
24	0.09023761663643601	0.0	0.0	0.0	0.0
25	0.09732576779216814	0.0	0.0	0.0	0.0
26	0.10277819175811594	0.0	0.0	0.0	0.0
27	0.10795799452576633	0.0	0.0	0.0	0.0
28	0.11822672632830135	0.0	0.0	0.0	0.0
29	0.12567837241509666	0.0	0.0	0.0	0.0
30	0.1332208922346578	0.0	0.0	0.0	0.0
31	0.13930943232996615	0.0	0.0	0.0	0.0
32	0.1486694268048432	0.0	0.0	0.0	0.0
33	0.1538492295724936	0.0	0.0	0.0	0.0
34	0.16084650699545994	0.0	0.0	0.0	0.0
35	0.17911212728138506	0.0	0.0	0.0	0.0
36	0.1890173641528569	0.0	0.0	0.0	0.0
37	0.19737774756731016	0.0	0.0	0.0	0.0
38	0.2075556056370794	0.0	0.0	0.0	0.0
39	0.21718822131025384	0.0	0.0	0.0	0.0
40	0.22782044804385204	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACGC	35	1.0195254E-7	46.000004	27
CCGTATT	35	1.0195254E-7	46.000004	45
TGTTACG	35	1.0195254E-7	46.000004	26
ATCGCTA	35	1.0195254E-7	46.000004	43
TCACGAA	40	5.6115823E-9	46.0	11
AACCGAT	60	0.0	46.0	40
ACGCATT	25	3.4170604E-5	46.0	22
CGACGAA	20	6.311337E-4	46.0	20
GCGCGTA	65	0.0	46.0	10
GACCGTT	20	6.311337E-4	46.0	46
CGGTTAG	25	3.4170604E-5	46.0	42
CGACCAA	30	1.86133E-6	46.0	31
CGTCTAA	50	1.6370905E-11	46.0	16
CACGATA	90	0.0	46.0	34
GTCTAAT	20	6.311337E-4	46.0	40
CACGAAT	45	3.092282E-10	46.0	44
TACGGAT	20	6.311337E-4	46.0	20
CGTTCAT	20	6.311337E-4	46.0	43
CGTTCAC	20	6.311337E-4	46.0	29
TTATATC	30	1.86133E-6	46.0	15
>>END_MODULE