FastQCFastQC Report
Fri 17 Jun 2016
SRR1527202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070424
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT38080.35574688160953044No Hit
TAGCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC27890.26055095924605576No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG27800.25971017092292403No Hit
TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC22070.20617998101686807No Hit
TAGCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG20650.19291420969634462No Hit
TAGCATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG18830.17591160138412443No Hit
TAGCATGGGATTCTATAAGGTCTGTTCTCTAAAGAACCTTAACTAATGCATC17620.16460766948424174No Hit
TAGCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT16950.1583484675231497No Hit
TAGCATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC15030.1404116499630053No Hit
TAGCATGGGACCACTCCCTGGCCAGGTCATCCATCACTGTCAGTCACTGTCT14320.13377876430274357No Hit
TAGCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC13600.12705245771768944No Hit
TAGCATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG13510.12621166939455766No Hit
TAGCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT12970.12116693945576706No Hit
TAGCATGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG12770.11929852095991869No Hit
TAGCATGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT12520.1169629978401082No Hit
TAGCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG11260.10519196131626347No Hit
TAGCATGGGAAGATCTTTGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTG10910.10192222894852881No Hit
TAGCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA10880.10164196617415155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA206.3112576E-446.00000434
TAGACGC405.6115823E-946.00000427
ACGCAAT206.3112576E-446.00000431
CGGTTAT206.3112576E-446.00000437
CGTCTAT351.019489E-746.00000433
CAATTCG206.3112576E-446.00000434
CGCGAAT206.3112576E-446.00000416
AAAACGA206.3112576E-446.00000435
CGTTATG206.3112576E-446.00000416
TACGCGT206.3112576E-446.00000431
TACGCAA206.3112576E-446.00000430
TCTACGA206.3112576E-446.00000413
TCTAACG206.3112576E-446.00000437
TATTATC206.3112576E-446.00000436
AATTCGA206.3112576E-446.00000435
CATACGT405.6115823E-946.00000411
CGTACGG206.3112576E-446.00000433
ACTCGCT351.019489E-746.00000421
TTACGCA206.3112576E-446.00000429
TATCGTG206.3112576E-446.00000421