##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527202_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1070424
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66305781634193	34.0	31.0	34.0	31.0	34.0
2	32.93309099945442	34.0	31.0	34.0	31.0	34.0
3	32.98859423929209	34.0	31.0	34.0	31.0	34.0
4	36.33128554666188	37.0	37.0	37.0	35.0	37.0
5	36.15206217349387	37.0	37.0	37.0	35.0	37.0
6	36.3020924418735	37.0	37.0	37.0	35.0	37.0
7	36.605858986719284	37.0	37.0	37.0	35.0	37.0
8	36.69600270546998	37.0	37.0	37.0	35.0	37.0
9	38.67800329589023	39.0	39.0	39.0	39.0	39.0
10	38.18200825093608	39.0	39.0	39.0	37.0	39.0
11	37.921856199038885	39.0	38.0	39.0	35.0	39.0
12	37.62844349528785	39.0	37.0	39.0	35.0	39.0
13	37.46618069101589	39.0	37.0	39.0	35.0	39.0
14	38.388925323049556	40.0	38.0	40.0	35.0	40.0
15	38.49051590771507	40.0	38.0	40.0	35.0	40.0
16	38.523711164921565	40.0	38.0	40.0	35.0	40.0
17	38.50651237266728	40.0	38.0	40.0	35.0	40.0
18	38.48795617437576	40.0	38.0	40.0	35.0	40.0
19	38.470916197693626	40.0	38.0	40.0	35.0	40.0
20	38.40171464765364	40.0	38.0	40.0	35.0	40.0
21	34.124488053332136	40.0	35.0	40.0	10.0	40.0
22	34.939322175138074	40.0	35.0	40.0	18.0	40.0
23	37.00879464585996	40.0	35.0	40.0	31.0	40.0
24	37.741910682122224	40.0	37.0	40.0	34.0	40.0
25	37.99150710372712	40.0	38.0	40.0	34.0	40.0
26	38.07177342809952	40.0	38.0	40.0	34.0	40.0
27	38.01989211751605	40.0	38.0	40.0	34.0	40.0
28	37.97745939926609	40.0	38.0	40.0	34.0	40.0
29	37.96687947953335	40.0	38.0	40.0	34.0	40.0
30	37.87316894987407	40.0	38.0	40.0	34.0	40.0
31	37.736488531647275	40.0	37.0	40.0	34.0	40.0
32	37.70657141469175	40.0	37.0	40.0	33.0	40.0
33	37.410665306458	40.0	37.0	40.0	33.0	40.0
34	37.42056325343976	40.0	37.0	40.0	33.0	40.0
35	37.3119950599015	40.0	36.0	40.0	33.0	40.0
36	37.19559352181939	40.0	36.0	40.0	33.0	40.0
37	37.07158845466843	39.0	35.0	40.0	32.0	40.0
38	37.01656259575645	39.0	35.0	40.0	32.0	40.0
39	36.864394856617565	39.0	35.0	40.0	32.0	40.0
40	31.87782504876572	38.0	31.0	40.0	9.0	40.0
41	32.86272168785453	38.0	31.0	40.0	15.0	40.0
42	34.70154910577491	38.0	32.0	40.0	25.0	40.0
43	35.55278095408922	37.0	34.0	40.0	29.0	40.0
44	36.25872177753862	38.0	35.0	40.0	31.0	40.0
45	36.51535092636189	39.0	35.0	40.0	31.0	40.0
46	36.525617885996574	39.0	35.0	40.0	32.0	40.0
47	36.48939392240832	39.0	35.0	40.0	32.0	40.0
48	36.36141940016292	38.0	35.0	40.0	31.0	40.0
49	36.22448207439295	38.0	35.0	40.0	31.0	40.0
50	36.11023202020881	38.0	35.0	40.0	31.0	40.0
51	35.92363399923769	37.0	35.0	40.0	31.0	40.0
52	35.54154708788293	37.0	34.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	14.0
17	35.0
18	104.0
19	207.0
20	343.0
21	632.0
22	1019.0
23	1620.0
24	2459.0
25	3368.0
26	4767.0
27	6701.0
28	9082.0
29	11855.0
30	15722.0
31	21243.0
32	29249.0
33	37998.0
34	65078.0
35	94439.0
36	144459.0
37	146530.0
38	274955.0
39	198539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.37052980874869	0.5924755050335194	0.019898656980785183	0.017096029237012624
8	99.54363878238904	0.396478404819025	0.029427591309611895	0.030455221482328494
9	97.63710454922536	1.5675096970919935	0.33099033653953946	0.4643954171431134
10	46.88347794892491	40.977687346322575	4.095947026598806	8.042887678153704
11	32.097187656480045	26.60833464122628	22.057427710888394	19.237049991405275
12	29.477478083451043	17.21682249276922	27.422404579867415	25.883294843912317
13	23.97059482971234	18.7931137567917	29.84733152470423	27.38895988879173
14	23.318049670037293	19.918088533142004	29.726258006173257	27.037603790647445
15	24.71422539105999	19.645392853672938	27.878765797478383	27.76161595778869
16	27.554875451223065	21.414691748316557	24.772800310904838	26.257632489555537
17	30.345265053847818	20.373702383354633	22.94137650127426	26.339656061523286
18	27.787867237655362	20.352028728802793	24.19611294216124	27.663991091380613
19	29.3104414699222	19.905663550144613	22.973233036628475	27.81066194330471
20	26.50155452418855	20.91965426784153	25.42422441948237	27.154566788487557
21	33.621443465393156	18.914093854397883	24.406216602019388	23.058246078189576
22	29.160127201931196	18.361789347025102	25.288483815758987	27.18959963528471
23	26.52948738070148	21.938969978251606	24.498516475714297	27.033026165332615
24	27.291615285158034	22.026318542932522	23.92509883933843	26.75696733257102
25	27.854756619806732	23.029192170579137	23.232008998303478	25.88404221131066
26	28.086533934216725	23.32262729535212	22.92661599515706	25.664222775274098
27	27.16577729946264	20.29205249508606	23.638763704849666	28.90340650060163
28	28.388657204995404	21.095192185526482	22.950998856527878	27.565151752950236
29	29.2487836595592	21.15806446791178	22.442415341957954	27.150736530571063
30	27.29899553821663	21.019614657369416	24.655276787515977	27.02611301689798
31	29.676277811409314	22.154118368048547	21.579953364274342	26.589650456267798
32	29.315859883560158	22.86598581496678	22.43951929328939	25.378635008183675
33	29.76250532499271	22.23137747285188	22.201389355993513	25.804727846161896
34	26.999674895181723	24.62099130811716	24.496928319992826	23.882405476708296
35	28.963476155243157	23.582991412748594	23.980871131439503	23.47266130056875
36	29.492051747718662	24.15575510265091	21.344532633797446	25.00766051583298
37	29.371632175661233	24.22675500549315	21.56594022555548	24.835672593290138
38	30.093682503381835	23.2908641809227	21.80762015799347	24.807833157701996
39	28.17257460595054	23.41100349020575	22.421115371105284	25.995306532738425
40	28.115681262751956	28.537663580039315	21.693833471596303	21.65282168561243
41	26.884393473987878	23.895204143404854	23.232662944777022	25.987739437830243
42	27.112060267707	22.632153240211355	23.641753174443025	26.61403331763862
43	27.240514039296578	21.993060693706422	24.682835960329736	26.083589306667264
44	25.139103757015913	22.95296069594852	26.004274941518503	25.903660605517064
45	27.027327488920278	22.037902737606778	24.771585838882537	26.163183934590407
46	26.459608528956753	23.75367144234434	24.25646285957714	25.530257169121768
47	26.596283341928057	23.125322302190536	24.27589441193396	26.002499943947445
48	27.841397427561414	23.255083966727202	23.7295688437479	25.17394976196348
49	26.72230816947303	23.32757860436612	23.410349543732202	26.539763682428646
50	25.181703698721254	24.62071104534278	23.906321233455156	26.29126402248081
51	25.400402083660307	24.405656076470635	24.11128674244972	26.082655097419337
52	25.62535967056045	25.379942901130764	22.39794698175676	26.59675044655202
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	39.0
8	77.0
9	509.0
10	941.0
11	1331.0
12	1721.0
13	2323.0
14	2339.0
15	1753.0
16	2294.5
17	2836.0
18	2885.0
19	2934.0
20	3134.0
21	3334.0
22	3058.5
23	2783.0
24	3362.0
25	3941.0
26	4952.0
27	5963.0
28	7494.0
29	9025.0
30	9711.0
31	10397.0
32	11419.0
33	12441.0
34	13804.0
35	15167.0
36	18555.0
37	21943.0
38	23872.5
39	25150.0
40	24498.0
41	26356.0
42	28214.0
43	33856.0
44	39498.0
45	40297.5
46	41097.0
47	44806.5
48	48516.0
49	50788.5
50	53061.0
51	56670.0
52	60279.0
53	63740.0
54	67201.0
55	73771.5
56	80342.0
57	88798.5
58	97255.0
59	92040.5
60	86826.0
61	84030.0
62	81234.0
63	75089.0
64	59887.0
65	50830.0
66	43177.5
67	35525.0
68	30210.5
69	24896.0
70	23352.5
71	21809.0
72	21217.5
73	20626.0
74	14961.0
75	9296.0
76	6643.0
77	3990.0
78	2921.5
79	1853.0
80	1159.0
81	465.0
82	306.0
83	147.0
84	91.0
85	35.0
86	18.0
87	1.0
88	0.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1070424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.062954592825212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.85655666255512	7.944246526602232
2	11.408825723118893	2.7524829331087894
3	5.554121722889349	2.0099735443871505
4	3.2684020518259542	1.5770634216909298
5	2.1178083766381244	1.2773513141845285
6	1.3741444320152207	0.9945745132429918
7	0.9417731692591429	0.7952396884259894
8	0.6931912075554387	0.6689547248709549
9	0.5178683466863153	0.5622320116024638
>10	3.9796632045947264	11.343751143953783
>50	2.237102152318334	19.67926052430718
>100	1.9596757531271676	41.13094850753044
>500	0.07558864209846276	6.083892173585684
>1k	0.015278555317774388	3.1800289725068875
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAGCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3808	0.35574688160953044	No Hit
TAGCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2789	0.26055095924605576	No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2780	0.25971017092292403	No Hit
TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2207	0.20617998101686807	No Hit
TAGCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2065	0.19291420969634462	No Hit
TAGCATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1883	0.17591160138412443	No Hit
TAGCATGGGATTCTATAAGGTCTGTTCTCTAAAGAACCTTAACTAATGCATC	1762	0.16460766948424174	No Hit
TAGCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1695	0.1583484675231497	No Hit
TAGCATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	1503	0.1404116499630053	No Hit
TAGCATGGGACCACTCCCTGGCCAGGTCATCCATCACTGTCAGTCACTGTCT	1432	0.13377876430274357	No Hit
TAGCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1360	0.12705245771768944	No Hit
TAGCATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	1351	0.12621166939455766	No Hit
TAGCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1297	0.12116693945576706	No Hit
TAGCATGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG	1277	0.11929852095991869	No Hit
TAGCATGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT	1252	0.1169629978401082	No Hit
TAGCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1126	0.10519196131626347	No Hit
TAGCATGGGAAGATCTTTGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTG	1091	0.10192222894852881	No Hit
TAGCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1088	0.10164196617415155	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.802627743772561E-4	0.0	0.0	0.0	0.0
8	3.736836991696748E-4	0.0	0.0	0.0	0.0
9	7.473673983393497E-4	0.0	0.0	0.0	0.0
10	0.009902618027996383	0.0	0.0	0.0	0.0
11	0.01718945016180504	0.0	0.0	0.0	0.0
12	0.021393391777463883	0.0	0.0	0.0	0.0
13	0.024849965994783375	0.0	0.0	0.0	0.0
14	0.03260390275255413	9.34209247924187E-5	0.0	0.0	0.0
15	0.03876968378885376	9.34209247924187E-5	0.0	0.0	0.0
16	0.04904598551601982	9.34209247924187E-5	0.0	0.0	0.0
17	0.054931503777942196	9.34209247924187E-5	0.0	0.0	0.0
18	0.05997623371673281	9.34209247924187E-5	0.0	0.0	0.0
19	0.06670254030178696	9.34209247924187E-5	0.0	0.0	0.0
20	0.07174727024057756	9.34209247924187E-5	0.0	0.0	0.0
21	0.0779130512768772	9.34209247924187E-5	0.0	0.0	0.0
22	0.08379856953879958	9.34209247924187E-5	0.0	0.0	0.0
23	0.09258013646928694	9.34209247924187E-5	0.0	0.0	0.0
24	0.09949328490392592	9.34209247924187E-5	0.0	0.0	0.0
25	0.10621959148898007	9.34209247924187E-5	0.0	0.0	0.0
26	0.11163800512694036	9.34209247924187E-5	0.0	0.0	0.0
27	0.11855115356157933	9.34209247924187E-5	0.0	0.0	0.0
28	0.12892087621353782	9.34209247924187E-5	0.0	0.0	0.0
29	0.1358340246481768	9.34209247924187E-5	0.0	0.0	0.0
30	0.14209322660926885	9.34209247924187E-5	0.0	0.0	0.0
31	0.14779190302160639	9.34209247924187E-5	0.0	0.0	0.0
32	0.1573208373504331	9.34209247924187E-5	0.0	0.0	0.0
33	0.16395372301069483	9.34209247924187E-5	0.0	0.0	0.0
34	0.1692787157238627	9.34209247924187E-5	0.0	0.0	0.0
35	0.1853471147881587	9.34209247924187E-5	0.0	0.0	0.0
36	0.19207342137321287	9.34209247924187E-5	0.0	0.0	0.0
37	0.20057472552932296	9.34209247924187E-5	0.0	0.0	0.0
38	0.2087957669110558	9.34209247924187E-5	0.0	0.0	0.0
39	0.21711022921758108	9.34209247924187E-5	0.0	0.0	0.0
40	0.22738653094474712	9.34209247924187E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	20	6.3112576E-4	46.000004	34
TAGACGC	40	5.6115823E-9	46.000004	27
ACGCAAT	20	6.3112576E-4	46.000004	31
CGGTTAT	20	6.3112576E-4	46.000004	37
CGTCTAT	35	1.019489E-7	46.000004	33
CAATTCG	20	6.3112576E-4	46.000004	34
CGCGAAT	20	6.3112576E-4	46.000004	16
AAAACGA	20	6.3112576E-4	46.000004	35
CGTTATG	20	6.3112576E-4	46.000004	16
TACGCGT	20	6.3112576E-4	46.000004	31
TACGCAA	20	6.3112576E-4	46.000004	30
TCTACGA	20	6.3112576E-4	46.000004	13
TCTAACG	20	6.3112576E-4	46.000004	37
TATTATC	20	6.3112576E-4	46.000004	36
AATTCGA	20	6.3112576E-4	46.000004	35
CATACGT	40	5.6115823E-9	46.000004	11
CGTACGG	20	6.3112576E-4	46.000004	33
ACTCGCT	35	1.019489E-7	46.000004	21
TTACGCA	20	6.3112576E-4	46.000004	29
TATCGTG	20	6.3112576E-4	46.000004	21
>>END_MODULE