FastQCFastQC Report
Fri 17 Jun 2016
SRR1527201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598816
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC45710.7633396569229947No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA36050.6020213220755625No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG34790.5809798001389408No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT32670.5455766044995458No Hit
CCGCTAGGGACTTTCCCTCCTGACAGATAGTCTAGAAGCCGATGTAGGAC24320.40613477261796616No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT21310.355868914658259No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC20090.3354953775450222No Hit
CCGCTAGGGGGCAGTGCAAAGTCCTTTTAGCCCCAGGCCGAGCCAGGCAT19530.3261435900176348No Hit
CCGCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT19210.3207997114305563No Hit
CCGCTAGGGATTCGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACA18380.30693902634532144No Hit
CCGCTAGGGATTGAATCTTCAGGACATTCAGTTCTAATACATTCTCTCTA18310.305770052904398No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC17830.29775423502378023No Hit
CCGCTAGGGATTGTGACCTTTGTATTCTATTCCGTGTGGTCACAGACACC17730.29608427296531825No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC16590.2770467054988511No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT16570.27671271308715867No Hit
CCGCTAGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGC14900.24882434671084272No Hit
CCGCTAGGGATATTTAGTGCTCTGTCTGCTGTCCCACTGTGTGGTCCAGT14240.2378025971249933No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC14210.23730160850745471No Hit
CCGCTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA13700.22878480200929835No Hit
CCGCTAGGGGCAGGAACCGCAAGTTCCAAGCTAGGTCGGAATTGGAATAG12980.2167610751883717No Hit
CCGCTAGGGATCATTAGTGATGTGTAGTCTCTCAGAGACTGATTACTGAC12670.21158419280713942No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG12440.20774328007267676No Hit
CCGCTAGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC12120.20239940148559826No Hit
CCGCTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA11940.19939346978036657No Hit
CCGCTAGGGGCAGGCTCCTACAGCGGCTCCTGGACGGAGGCCAAGCGCCG11930.1992264735745204No Hit
CCGCTAGGGGCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACA11640.1943835836049805No Hit
CCGCTAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC10530.17584700475605194No Hit
CCGCTAGGGGGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCT10520.17568000855020574No Hit
CCGCTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC10460.17467803131512852No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG10180.17000213755143484No Hit
CCGCTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC9780.1633222893175867No Hit
CCGCTAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA9780.1633222893175867No Hit
CCGCTAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC9760.16298829690589428No Hit
CCGCTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC9750.1628213007000481No Hit
CCGCTAGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAA9720.1623203120825095No Hit
CCGCTAGGGTGTGGATGTCCTGGCACCTACCGTTCAAGAGTTGGCTGCCC9600.16031635761235505No Hit
CCGCTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT9180.15330251696681452No Hit
CCGCTAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT9150.1528015283492759No Hit
CCGCTAGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGG9060.15129856249666007No Hit
CCGCTAGGGGTCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATG9000.15029658526158285No Hit
CCGCTAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG8810.147123657350505No Hit
CCGCTAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT8740.14595468390958158No Hit
CCGCTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC8640.14428472185111954No Hit
CCGCTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT8620.14395072943942713No Hit
CCGCTAGGGGGGTCAAGCCGCTGACTATTTAAGGGTCAAGTGGAACTTTT8520.1422807673809651No Hit
CCGCTAGGGACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTG8400.14027681291081065No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT8360.13960882808742586No Hit
CCGCTAAGGGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATAT8340.13927483567573345No Hit
CCGCTAGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCC8170.136435900176348No Hit
CCGCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC8140.13593491155880938No Hit
CCGCTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG8100.13526692673542456No Hit
CCGCTAGGGGCATTCAACCCCAGATGGGGTCAAGATAGTAATAGAAAAGG8070.13476593811788598No Hit
CCGCTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT7800.13025704056003848No Hit
CCGCTAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC7690.12842008229573024No Hit
CCGCTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG7600.12691711644311443No Hit
CCGCTAGGGGGCCATTTCGCCGCTGCTTCCATGCGGGTGAGGTGTGTTCT7530.125748143002191No Hit
CCGCTAGGGTGTCCATTTGTCGGACATGGTGGTTAAATGTTATCAGTGTA7470.12474616576711377No Hit
CCGCTAGGGGTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAAC7450.12441217335542136No Hit
CCGCTAGGGATTACCAAAGGACTCGACCAGACTAGGGTTCTTCTTAATTG7420.12391118473788275No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCC7270.1214062416501897No Hit
CCGCTAGGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCC7190.12007027200342008No Hit
CCGCTAGGGGGACATCAGCTGCCGCGGCGATGGACGATTTACTGGACCTG7000.11689734409234222No Hit
CCGCTAGGGGAGGGGACAGTCACTCTCCTGGTCACAAGAGGAAGGAGACA6970.11639635547480362No Hit
CCGCTAGGGACCACCCCTATCTTCTCCCCCCACCCCATACTCTATCCCTC6910.11539437823972638No Hit
CCGCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG6740.11255544274034093No Hit
CCGCTAGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCT6720.11222145032864853No Hit
CCGCTAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA6720.11222145032864853No Hit
CCGCTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG6630.1107184844760327No Hit
CCGCTAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA6500.10854753380003207No Hit
CCGCTAGGGGGTTTCCGGTGCGGGCAGTCGCAGCCGGCGGTAAAGCCTTG6430.10737856035910864No Hit
CCGCTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC6360.10620958691818522No Hit
CCGCTAGGGGTGCAGTGCTCCGCTAGGGGGGGCTGGAGAGTTGGCTCAGC6250.10437262865387698No Hit
CCGCTAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCT6240.10420563244803079No Hit
CCGCTAGGGGACTTCGCCCTCCAGCCGCGGTGGCTGCAGCGCAACTTTCA6160.10286966280126114No Hit
CCGCTAGGGGGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAG6130.10236867418372256No Hit
CCGCTAGGGAGATGCAGTTGTATAAGCTCGGATAAATGGATAAAGCCGAG6120.10220167797787634No Hit
CCGCTAGGGGAGCGCAACTTTCAGATAGCGGAGTGGCCTCAGCTGCGAGC6050.10103270453695291No Hit
CCGCTAGGGCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG6020.1005317159194143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC408.301868E-944.00000414
TCTGCGA207.853456E-444.00000423
ATGGGTT207.853456E-444.00000416
TAGAGTC207.853456E-444.00000425
AACGTCA207.853456E-444.00000417
AATCCCG207.853456E-444.00000434
ACACGGA408.301868E-944.00000428
ACACGCG207.853456E-444.00000430
ACGCCCC207.853456E-444.00000416
GTACATT207.853456E-444.00000425
TCACCAA408.301868E-944.00000415
ATTAGCT207.853456E-444.00000410
CTATCTC207.853456E-444.00000420
ACGTTGC207.853456E-444.00000418
ACCATAA207.853456E-444.00000436
TCCGAAT207.853456E-444.00000423
GTTTACT408.301868E-944.00000418
ATCAGTA207.853456E-444.00000432
ATAATAT207.853456E-444.00000432
TTATGGG207.853456E-444.00000430
Produced by FastQC (version 0.11.5)