FastQCFastQC Report
Fri 17 Jun 2016
SRR1527200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649630
Sequences flagged as poor quality0
Sequence length52
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT41270.6352847005218355No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC33390.513984883703031No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG32350.49797577082339184No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG31210.48042732016686424No Hit
CCGCTAGGGACTTTCCCTCCTGACAGATAGTCTAGAAGCCGATGTAGGACCG22270.3428105229130428No Hit
CCGCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC22100.34019364869233254No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA20550.3163339131505626No Hit
CCGCTAGGGGGCAGTGCAAAGTCCTTTTAGCCCCAGGCCGAGCCAGGCATCT18930.2913966411649708No Hit
CCGCTAGGGATTGTGACCTTTGTATTCTATTCCGTGTGGTCACAGACACCTG18900.2909348398319043No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA18680.28754829672274984No Hit
CCGCTAGGGATTCGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATG18050.27785046872835306No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT17630.27138525006542186No Hit
CCGCTAGGGATTGAATCTTCAGGACATTCAGTTCTAATACATTCTCTCTAGT16830.2590705478503148No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC16770.2581469451841818No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC15660.2410602958607207No Hit
CCGCTAGGGGCAGGAACCGCAAGTTCCAAGCTAGGTCGGAATTGGAATAGTC14970.23043886520019088No Hit
CCGCTAGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTC14400.22166463987192708No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA14190.21843203054046148No Hit
CCGCTAGGGATATTTAGTGCTCTGTCTGCTGTCCCACTGTGTGGTCCAGTCC13920.21427581854286284No Hit
CCGCTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA13140.2022689838831335No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT12680.19518803010944694No Hit
CCGCTAGGGGCAGGCTCCTACAGCGGCTCCTGGACGGAGGCCAAGCGCCGCG12340.18995428166802641No Hit
CCGCTAGGGATCATTAGTGATGTGTAGTCTCTCAGAGACTGATTACTGACTC11920.1834890630050952No Hit
CCGCTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA11540.17763957945291936No Hit
CCGCTAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC10740.1653248772378123No Hit
CCGCTAGGGGCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACG10680.16440127457167927No Hit
CCGCTAGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT10560.1625540692394132No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10290.15839785724181457No Hit
CCGCTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG9900.15239443991194987No Hit
CCGCTAGGGTGTGGATGTCCTGGCACCTACCGTTCAAGAGTTGGCTGCCCTT9780.1505472345796838No Hit
CCGCTAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA9540.1468528239151517No Hit
CCGCTAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG9400.14469775102750798No Hit
CCGCTAGGGGGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCA9350.14392808213906377No Hit
CCGCTAGGGGGGTCAAGCCGCTGACTATTTAAGGGTCAAGTGGAACTTTTCA9130.14054153902990932No Hit
CCGCTAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG9110.14023367147453167No Hit
CCGCTAGGGGTCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAA9070.1396179363637763No Hit
CCGCTAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG9000.13854039991995443No Hit
CCGCTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT8950.13777073103151025No Hit
CCGCTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8700.1339223865892893No Hit
CCGCTAGGGGCATTCAACCCCAGATGGGGTCAAGATAGTAATAGAAAAGGGG8580.13207518125702322No Hit
CCGCTAGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACA8510.13099764481320136No Hit
CCGCTAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA8470.130381909702446No Hit
CCGCTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA8310.1279189692594246No Hit
CCGCTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT8230.12668749903791388No Hit
CCGCTAGGGACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTG8060.12407062481720364No Hit
CCGCTAGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCC8040.12376275726182596No Hit
CCGCTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT8030.12360882348413711No Hit
CCGCTAAGGGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATATCT7870.1211458830411157No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC7750.11929867770884965No Hit
CCGCTAGGGGAGGGGACAGTCACTCTCCTGGTCACAAGAGGAAGGAGACACC7720.11883687637578315No Hit
CCGCTAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC7670.11806720748733894No Hit
CCGCTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT7620.11729753859889476No Hit
CCGCTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC7600.1169896710435171No Hit
CCGCTAGGGGTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT7520.11575820082200639No Hit
CCGCTAGGGGGACATCAGCTGCCGCGGCGATGGACGATTTACTGGACCTGGG7430.11437279682280682No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT7210.1109862537136524No Hit
CCGCTAGGGTGTCCATTTGTCGGACATGGTGGTTAAATGTTATCAGTGTAGC6960.10713790927143142No Hit
CCGCTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT6850.10544463771685422No Hit
CCGCTAGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCTT6820.10498283638378769No Hit
CCGCTAGGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCAA6580.10128842571925559No Hit
CCGCTAGGGGGCCATTTCGCCGCTGCTTCCATGCGGGTGAGGTGTGTTCTTT6520.10036482305312254No Hit
CCGCTAGGGGTGTCCATGGAAAAGGTCCCACAGAGCAGCTTGTCTCCCCAGA6500.10005695549774488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA3150.046.00000440
TAATCAT206.3094177E-446.033
ACGCGAA206.3094177E-446.022
TTGATGA206.3094177E-446.018
ATTTACA206.3094177E-446.023
CTTAACA301.860144E-646.029
GTCATAT301.860144E-646.012
CTAGACT453.092282E-1046.036
GTACCTC206.3094177E-446.032
TGATACG301.860144E-646.013
CTCACGT253.415504E-546.031
GATACAT405.6043064E-946.022
GGTACGT206.3094177E-446.019
ACAAATC206.3094177E-446.044
GATACAC206.3094177E-446.09
CAAACTA206.3094177E-446.023
AAATCGC301.860144E-646.035
ACAAACG206.3094177E-446.034
GATAATC253.415504E-546.032
ATGATCT301.860144E-646.013
Produced by FastQC (version 0.11.5)