FastQCFastQC Report
Fri 17 Jun 2016
SRR1527199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629552
Sequences flagged as poor quality0
Sequence length52
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT44140.7011335044603146No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG33420.5308536864309858No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC30970.4919371235418202No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG29800.47335247922332074No Hit
CCGCTAGGGACTTTCCCTCCTGACAGATAGTCTAGAAGCCGATGTAGGACCG23350.37089867080082345No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA19090.30323150430782525No Hit
CCGCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC18800.29862505400665873No Hit
CCGCTAGGGGGCAGTGCAAAGTCCTTTTAGCCCCAGGCCGAGCCAGGCATCT18790.2984662108928254No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA17480.27765776298065925No Hit
CCGCTAGGGATTGAATCTTCAGGACATTCAGTTCTAATACATTCTCTCTAGT17140.2722570971103261No Hit
CCGCTAGGGATTCGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATG17080.2713040384273261No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC16230.2578023737514931No Hit
CCGCTAGGGATTGTGACCTTTGTATTCTATTCCGTGTGGTCACAGACACCTG16200.2573258444099931No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT15990.2539901390194932No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC15140.24048847434366025No Hit
CCGCTAGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTC14530.2307990443998272No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA13040.20713142043866112No Hit
CCGCTAGGGGCAGGAACCGCAAGTTCCAAGCTAGGTCGGAATTGGAATAGTC12900.20490761684499453No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT12480.19823620606399472No Hit
CCGCTAGGGATATTTAGTGCTCTGTCTGCTGTCCCACTGTGTGGTCCAGTCC12470.1980773629501614No Hit
CCGCTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA11890.1888644623478283No Hit
CCGCTAGGGGCAGGCTCCTACAGCGGCTCCTGGACGGAGGCCAAGCGCCGCG11120.17663354258266195No Hit
CCGCTAGGGGCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACG11020.1750451114443287No Hit
CCGCTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA10890.17298015096449537No Hit
CCGCTAGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT10710.17012097491549547No Hit
CCGCTAGGGATCATTAGTGATGTGTAGTCTCTCAGAGACTGATTACTGACTC10480.16646758329732889No Hit
CCGCTAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC9850.15646046712582917No Hit
CCGCTAGGGTGTGGATGTCCTGGCACCTACCGTTCAAGAGTTGGCTGCCCTT9380.1489948407756627No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG9380.1489948407756627No Hit
CCGCTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG9360.14867715454799602No Hit
CCGCTAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG9160.14550029227132946No Hit
CCGCTAGGGGGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCA8750.13898772460416298No Hit
CCGCTAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG8710.13835235214882963No Hit
CCGCTAGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACA8710.13835235214882963No Hit
CCGCTAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA8670.13771697969349633No Hit
CCGCTAGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCC8510.13517548987216307No Hit
CCGCTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT8380.13311052939232978No Hit
CCGCTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT8340.13247515693699646No Hit
CCGCTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT8280.1315220982539965No Hit
CCGCTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8250.1310455689124965No Hit
CCGCTAGGGGTCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAA8180.1299336671156632No Hit
CCGCTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT8060.12802754974966324No Hit
CCGCTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA8030.12755102040816327No Hit
CCGCTAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG7860.12485068747299667No Hit
CCGCTAGGGGGGTCAAGCCGCTGACTATTTAAGGGTCAAGTGGAACTTTTCA7820.12421531501766335No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC7740.12294457010699673No Hit
CCGCTAGGGACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTG7710.12246804076549674No Hit
CCGCTAGGGGCATTCAACCCCAGATGGGGTCAAGATAGTAATAGAAAAGGGG7480.11881464914733017No Hit
CCGCTAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA7360.11690853178133022No Hit
CCGCTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC7270.11547894375683027No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT7160.11373166950466364No Hit
CCGCTAAGGGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATATCT7150.11357282639083031No Hit
CCGCTAGGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCAA6950.11039596411416372No Hit
CCGCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG6840.10864868986199709No Hit
CCGCTAGGGGTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT6830.10848984674816378No Hit
CCGCTAGGGGGACATCAGCTGCCGCGGCGATGGACGATTTACTGGACCTGGG6820.10833100363433046No Hit
CCGCTAGGGGGCCATTTCGCCGCTGCTTCCATGCGGGTGAGGTGTGTTCTTT6800.10801331740666378No Hit
CCGCTAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC6600.1048364551299972No Hit
CCGCTAGGGGAGGGGACAGTCACTCTCCTGGTCACAAGAGGAAGGAGACACC6550.10404223956083054No Hit
CCGCTAGGGTGTCCATTTGTCGGACATGGTGGTTAAATGTTATCAGTGTAGC6440.10229496530866394No Hit
CCGCTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT6430.1021361221948306No Hit
CCGCTAGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTCT6410.10181843596716396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTATT1150.046.00000423
CTTGTAA551.8189894E-1246.017
ATTTACA206.309269E-446.023
GTCATAT206.309269E-446.012
AACGTAT206.309269E-446.039
AGCGTAA206.309269E-446.011
AGGTACC253.4153833E-546.011
ACTATAC206.309269E-446.041
GGTACGT253.4153833E-546.019
AAATCGC206.309269E-446.035
CGAACGA206.309269E-446.032
GATGTTA501.6370905E-1146.09
CATATTC206.309269E-446.038
CATATGA600.046.031
ACGCCCT206.309269E-446.025
GATCGTA206.309269E-446.042
CGTATCG253.4153833E-546.015
CGTATCC206.309269E-446.044
GATAAGC253.4153833E-546.042
TTAATAA253.4153833E-546.025
Produced by FastQC (version 0.11.5)