FastQCFastQC Report
Fri 17 Jun 2016
SRR1527198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701597
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC78970.46409343693013094No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC76470.44940135649040286No Hit
TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT70420.41384652182626086No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC55000.323225769674018No Hit
TACTTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG54490.32022858526431347No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC53710.3156446561671183No Hit
TACTTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC51390.3020104055190506No Hit
TACTTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA50370.2960160366996416No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG49830.2928425473246603No Hit
TACTTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG38560.22661064870236608No Hit
TACTTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT36570.2149157526723425No Hit
TACTTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG33340.19593358474421382No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGA32800.19276009536923255No Hit
TACTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA32600.19158472893405432No Hit
TACTTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT31820.18700079983685916No Hit
TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC30130.17706895345960294No Hit
TACTTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA27780.16325839784625854No Hit
TACTTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT27080.15914461532313467No Hit
TACTTCGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTG25860.15197488006854737No Hit
TACTTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT24940.14656819446672742No Hit
TACTTCGGGATTCAAATGACTGGAGATCTGGGAGATTTTTGGAGTGCTTC23220.1364600431241945No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23080.13563728661956975No Hit
TACTTCGGGATTCTATGAAAGTTGAGTTAAATGATAGCTAAAATATCTGT22890.1345206885061504No Hit
TACTTCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC22550.13252256556634737No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT22000.1292903078696072No Hit
TACTTCGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGA21100.12400115891130509No Hit
TACTTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC20920.12294332911964466No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA20890.12276702415436794No Hit
TACTTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG20510.12053382792752926No Hit
TACTTCGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAA19760.11612620379561082No Hit
TACTTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC19680.11565605722153953No Hit
TACTTCGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT19550.11489206903867366No Hit
TACTTCGGAGAATTTTTTTAACTCTAGCACAAAGCTCTTGCTCATGAAAT19510.11465699575163803No Hit
TACTTCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG19260.1131877877076652No Hit
TACTTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG19040.11189488462896914No Hit
TACTTCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA18940.11130720141138001No Hit
TACTTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC18710.10995553001092503No Hit
TACTTCGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA18670.10972045672388937No Hit
TACTTCGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGC18470.10854509028871114No Hit
TACTTCGGGACTACACAGCAAATCTACGTCAGTGTGTCCTGAGTGAGGCA17590.10337347797392685No Hit
TACTTCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC17490.10278579475633773No Hit
TACTTCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC17380.10213934321698968No Hit
TACTTCGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGC17360.10202180657347187No Hit
TACTTCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC17220.1011990500688471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAG650.044.00000423
TACGTCA2200.044.00000425
ATTTACG302.528437E-644.020
GTACCGT351.4466423E-744.022
GTATTAC302.528437E-644.030
ATAGGGC207.857551E-444.037
CGAAACT207.857551E-444.022
ACGTTAC207.857551E-444.022
ATTACCG207.857551E-444.034
CCAATTA207.857551E-444.034
TAACGGC502.7284841E-1144.025
TAACGCG207.857551E-444.020
GTTACGT254.4437445E-544.039
CCGTATA454.802132E-1044.017
CTAGTAG207.857551E-444.013
TCGTCAA351.4466423E-744.020
AATCTCG302.528437E-644.027
AACGATA207.857551E-444.044
AAACACG254.4437445E-544.042
AACGAAC454.802132E-1044.012
Produced by FastQC (version 0.11.5)