##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527198_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1701597
Sequences flagged as poor quality	0
Sequence length	50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62207855326496	34.0	31.0	34.0	31.0	34.0
2	32.94177763595023	34.0	31.0	34.0	31.0	34.0
3	33.06117782295103	34.0	33.0	34.0	31.0	34.0
4	36.474014117326256	37.0	37.0	37.0	35.0	37.0
5	36.48482631316346	37.0	37.0	37.0	35.0	37.0
6	36.32493357710433	37.0	37.0	37.0	35.0	37.0
7	36.54736579812964	37.0	37.0	37.0	35.0	37.0
8	36.66019451139136	37.0	37.0	37.0	35.0	37.0
9	38.68137461455327	39.0	39.0	39.0	39.0	39.0
10	38.16828602777273	39.0	39.0	39.0	37.0	39.0
11	37.90021491575267	39.0	38.0	39.0	35.0	39.0
12	37.51515840707288	39.0	37.0	39.0	35.0	39.0
13	37.39508826120403	39.0	37.0	39.0	35.0	39.0
14	38.221442562486885	40.0	38.0	40.0	35.0	40.0
15	38.1879075950416	40.0	38.0	40.0	34.0	40.0
16	38.07105619015548	40.0	38.0	40.0	34.0	40.0
17	38.15862862945809	40.0	38.0	40.0	34.0	40.0
18	38.10857858823211	40.0	38.0	40.0	34.0	40.0
19	38.02055539590162	40.0	38.0	40.0	34.0	40.0
20	37.817245211410224	40.0	38.0	40.0	33.0	40.0
21	37.98948340882124	40.0	38.0	40.0	34.0	40.0
22	37.81029938346153	40.0	38.0	40.0	34.0	40.0
23	37.800660203326636	40.0	37.0	40.0	34.0	40.0
24	37.79362152142957	40.0	37.0	40.0	34.0	40.0
25	37.69395456150898	40.0	37.0	40.0	34.0	40.0
26	37.52676573830349	40.0	37.0	40.0	33.0	40.0
27	37.38247951777066	40.0	36.0	40.0	33.0	40.0
28	37.19599059001632	39.0	36.0	40.0	33.0	40.0
29	37.41986968712333	40.0	36.0	40.0	33.0	40.0
30	37.42144467814647	40.0	36.0	40.0	33.0	40.0
31	37.36179483156118	40.0	36.0	40.0	33.0	40.0
32	36.59991819449611	40.0	35.0	40.0	31.0	40.0
33	36.70793143147291	39.0	35.0	40.0	31.0	40.0
34	36.64145740736496	39.0	35.0	40.0	31.0	40.0
35	36.58890442331528	39.0	35.0	40.0	31.0	40.0
36	36.304201288554225	39.0	35.0	40.0	31.0	40.0
37	36.147540810191835	39.0	35.0	40.0	30.0	40.0
38	35.88094360768149	39.0	35.0	40.0	30.0	40.0
39	35.75226507804139	38.0	35.0	40.0	29.0	40.0
40	35.57183046279466	38.0	35.0	40.0	29.0	40.0
41	35.42362909666625	37.0	35.0	40.0	29.0	40.0
42	35.25244050148184	37.0	35.0	40.0	28.0	40.0
43	35.115055444973166	37.0	35.0	40.0	28.0	40.0
44	34.90445857626688	37.0	34.0	40.0	27.0	40.0
45	34.77723456259032	36.0	34.0	40.0	27.0	40.0
46	34.42782750557271	35.0	34.0	40.0	26.0	40.0
47	34.28573510649114	35.0	34.0	39.0	26.0	40.0
48	34.08148874263413	35.0	34.0	39.0	26.0	40.0
49	33.75074532924071	35.0	33.0	39.0	24.0	40.0
50	33.36134877999903	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	4.0
15	13.0
16	60.0
17	174.0
18	370.0
19	761.0
20	1478.0
21	2254.0
22	3307.0
23	4510.0
24	6368.0
25	8800.0
26	11515.0
27	15135.0
28	19972.0
29	24679.0
30	29782.0
31	36244.0
32	44047.0
33	57795.0
34	97328.0
35	132819.0
36	184165.0
37	320439.0
38	482980.0
39	216595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.46085941618374	0.34943644117849293	0.1279386364691522	0.06176550616861689
8	98.96620645193897	0.7880831947870148	0.0989070855202495	0.14680326775376307
9	94.55470361078446	3.257057928522441	1.0617672692182696	1.1264711914748322
10	52.83718765371589	30.791603417260372	6.474447239857615	9.896761689166118
11	33.073988729411255	23.223771551078194	20.639845979982336	23.06239373952822
12	27.214375671795377	17.612337116250202	30.573337870247773	24.599949341706644
13	23.076321831785084	17.61962438814831	32.380404996012565	26.923648784054038
14	24.731414077481332	18.670695822806458	29.9299422836312	26.66794781608101
15	23.686689621573144	19.16258667592855	29.232127231065874	27.918596471432426
16	29.568634641457407	18.596412664103195	26.338139994369996	25.496812700069405
17	30.851899715385017	18.402829812229335	24.062336734256114	26.682933738129535
18	30.711854804633532	19.39724858471189	23.26755395078858	26.623342659865994
19	30.274148344173152	19.52307156159772	22.108348804094035	28.09443129013509
20	28.807643643001253	21.24950854990929	23.0342437134057	26.908604093683756
21	30.69081574544384	19.24221775191188	25.53083955836782	24.536126944276464
22	32.07810074888472	18.881674097920953	22.331080743560314	26.709144409634007
23	29.687111578123375	20.950730402086982	22.610347808558664	26.751810211230982
24	30.02250239040149	19.559625457731766	23.09318834012989	27.324683811736854
25	28.013683616038342	18.837245246671216	23.790004331225315	29.359066806065126
26	28.922712017005203	18.85646248788638	23.10253250328956	29.118292991818862
27	29.04512643122902	17.35969210100864	23.58425643674736	30.010925031014985
28	29.543070421492278	19.745157049524654	22.615754494160488	28.096018034822585
29	30.34566939175375	20.182863509985033	22.03729790308751	27.43416919517371
30	30.31804828052706	20.634145452771723	21.516845645590582	27.53096062111064
31	32.65156203260819	20.956372160975835	20.043347514129373	26.3487182922866
32	33.96526909720692	20.42675204528452	21.71324937690887	23.894729480599697
33	32.46832240536391	19.850940028690694	22.06115784172163	25.619579724223772
34	30.0651094236767	22.73758122516671	23.772079993088845	23.425229358067746
35	30.66695580680972	23.32608719926046	23.6221032359601	22.38485375796972
36	31.900973027103362	23.628802824640616	20.285708073063127	24.184516075192892
37	31.728076624488644	24.35935183242566	20.63567342913745	23.27689811394825
38	32.92072094626401	23.062099897919424	20.304925314278293	23.71225384153827
39	30.986949318786998	22.620749801509994	20.972180839528985	25.420120040174027
40	29.470315239154747	23.25668181126318	22.29011922329435	24.982883726287717
41	27.7783164873939	22.79546802209924	21.934923486583486	27.491292003923373
42	27.50163522855294	21.938332049245503	23.72477149407292	26.835261228128633
43	25.894321628446686	21.223297878404814	25.117404414793864	27.764976078354632
44	26.66677244964583	20.65841676965815	25.119343769411913	27.555467011284108
45	27.302645691077267	21.817739452996214	24.24827970430131	26.63133515162521
46	28.1986862929354	21.977883129789248	22.709078589113638	27.11435198816171
47	28.05182425685988	22.232761341257653	23.22024545177266	26.49516895010981
48	28.394678646001374	23.075616611923973	23.875335934419255	24.6543688076554
49	27.84966123000922	22.677049853755033	23.856471303134644	25.616817613101105
50	25.667769748066082	24.07056429930236	23.42769762758162	26.833968325049938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	17.5
4	35.0
5	120.5
6	206.0
7	292.5
8	379.0
9	1735.5
10	3092.0
11	2861.0
12	2630.0
13	2583.0
14	2536.0
15	2472.0
16	2408.0
17	2546.0
18	2684.0
19	3686.0
20	4688.0
21	4943.5
22	5199.0
23	6542.0
24	7885.0
25	8172.0
26	8459.0
27	10836.5
28	13214.0
29	15109.5
30	17005.0
31	18552.0
32	20099.0
33	21825.0
34	23551.0
35	23019.0
36	22487.0
37	25536.5
38	28586.0
39	29007.0
40	29428.0
41	33040.5
42	36653.0
43	40033.5
44	43414.0
45	43297.0
46	43180.0
47	46596.0
48	50012.0
49	56817.0
50	63622.0
51	77479.0
52	91336.0
53	97717.0
54	104098.0
55	132689.0
56	161280.0
57	168828.5
58	176377.0
59	171392.0
60	166407.0
61	162905.0
62	159403.0
63	144623.0
64	129843.0
65	109971.0
66	90099.0
67	78152.0
68	66205.0
69	56414.5
70	46624.0
71	37946.5
72	29269.0
73	31398.0
74	33527.0
75	22059.5
76	10592.0
77	6756.0
78	2920.0
79	2270.5
80	1621.0
81	1036.0
82	451.0
83	256.5
84	62.0
85	40.5
86	19.0
87	14.5
88	10.0
89	5.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1701597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.748497766097874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.72230699483055	6.7968775398629555
2	10.33044611087698	2.0141266166935736
3	4.674047234466753	1.3669481707150526
4	2.6393302718437655	1.0291802103625374
5	1.5996413574158215	0.7797050099662951
6	1.018322610030367	0.5956269413428792
7	0.6963367256070073	0.4751765909822273
8	0.46771327544224495	0.3647601456658435
9	0.3983565533825042	0.3495040173683811
>10	3.7069891121514225	8.695546330673137
>50	2.166529736779009	15.457898439294809
>100	2.4166106054675374	43.00245395583199
>500	0.10681846150012515	7.077297581109786
>1k	0.051524199076530955	8.988425195188245
>5k	0.005026751129417655	3.006473254942276
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	7897	0.46409343693013094	No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	7647	0.44940135649040286	No Hit
TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	7042	0.41384652182626086	No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5500	0.323225769674018	No Hit
TACTTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	5449	0.32022858526431347	No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	5371	0.3156446561671183	No Hit
TACTTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	5139	0.3020104055190506	No Hit
TACTTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	5037	0.2960160366996416	No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4983	0.2928425473246603	No Hit
TACTTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	3856	0.22661064870236608	No Hit
TACTTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	3657	0.2149157526723425	No Hit
TACTTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	3334	0.19593358474421382	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGA	3280	0.19276009536923255	No Hit
TACTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3260	0.19158472893405432	No Hit
TACTTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT	3182	0.18700079983685916	No Hit
TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	3013	0.17706895345960294	No Hit
TACTTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	2778	0.16325839784625854	No Hit
TACTTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	2708	0.15914461532313467	No Hit
TACTTCGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTG	2586	0.15197488006854737	No Hit
TACTTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	2494	0.14656819446672742	No Hit
TACTTCGGGATTCAAATGACTGGAGATCTGGGAGATTTTTGGAGTGCTTC	2322	0.1364600431241945	No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2308	0.13563728661956975	No Hit
TACTTCGGGATTCTATGAAAGTTGAGTTAAATGATAGCTAAAATATCTGT	2289	0.1345206885061504	No Hit
TACTTCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC	2255	0.13252256556634737	No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2200	0.1292903078696072	No Hit
TACTTCGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGA	2110	0.12400115891130509	No Hit
TACTTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	2092	0.12294332911964466	No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	2089	0.12276702415436794	No Hit
TACTTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	2051	0.12053382792752926	No Hit
TACTTCGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAA	1976	0.11612620379561082	No Hit
TACTTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1968	0.11565605722153953	No Hit
TACTTCGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	1955	0.11489206903867366	No Hit
TACTTCGGAGAATTTTTTTAACTCTAGCACAAAGCTCTTGCTCATGAAAT	1951	0.11465699575163803	No Hit
TACTTCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG	1926	0.1131877877076652	No Hit
TACTTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	1904	0.11189488462896914	No Hit
TACTTCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA	1894	0.11130720141138001	No Hit
TACTTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	1871	0.10995553001092503	No Hit
TACTTCGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA	1867	0.10972045672388937	No Hit
TACTTCGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGC	1847	0.10854509028871114	No Hit
TACTTCGGGACTACACAGCAAATCTACGTCAGTGTGTCCTGAGTGAGGCA	1759	0.10337347797392685	No Hit
TACTTCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1749	0.10278579475633773	No Hit
TACTTCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1738	0.10213934321698968	No Hit
TACTTCGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGC	1736	0.10202180657347187	No Hit
TACTTCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	1722	0.1011990500688471	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.5260993055347416E-4	0.0	0.0	0.0	0.0
8	0.0022919645485975824	0.0	0.0	0.0	0.0
9	0.0036436359490525667	0.0	0.0	0.0	0.0
10	0.02039260765034259	0.0	0.0	0.0	0.0
11	0.036142517881731104	0.0	0.0	0.0	0.0
12	0.04918908531220965	0.0	0.0	0.0	0.0
13	0.0594147732982604	0.0	0.0	0.0	0.0
14	0.0789258561222193	0.0	0.0	0.0	0.0
15	0.10008245195542775	0.0	0.0	0.0	0.0
16	0.11924092484883318	0.0	0.0	0.0	0.0
17	0.13410931025383802	0.0	0.0	0.0	0.0
18	0.14509898642275462	0.0	0.0	0.0	0.0
19	0.1621417997328392	0.0	0.0	0.0	0.0
20	0.1768338801725673	0.0	0.0	0.0	0.0
21	0.18976291095952802	0.0	0.0	0.0	0.0
22	0.20263317342472983	0.0	0.0	0.0	0.0
23	0.2177366321167703	0.0	0.0	0.0	0.0
24	0.23372161563519447	0.0	0.0	0.0	0.0
25	0.24694448803094976	0.0	0.0	0.0	0.0
26	0.26210671504474914	0.0	0.0	0.0	0.0
27	0.2807950413640833	0.0	0.0	0.0	0.0
28	0.30959151902595033	0.0	0.0	0.0	0.0
29	0.321874098273563	0.0	0.0	0.0	0.0
30	0.3363898737480144	0.0	0.0	0.0	0.0
31	0.3503767343266355	0.0	0.0	0.0	0.0
32	0.36501004644460466	0.0	0.0	0.0	0.0
33	0.37570588100472674	0.0	0.0	0.0	0.0
34	0.3910444129838029	0.0	0.0	0.0	0.0
35	0.42883244387478353	0.0	0.0	0.0	0.0
36	0.44469989074968985	0.0	0.0	0.0	0.0
37	0.4582753730759986	0.0	0.0	0.0	0.0
38	0.47402528330738714	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAG	65	0.0	44.000004	23
TACGTCA	220	0.0	44.000004	25
ATTTACG	30	2.528437E-6	44.0	20
GTACCGT	35	1.4466423E-7	44.0	22
GTATTAC	30	2.528437E-6	44.0	30
ATAGGGC	20	7.857551E-4	44.0	37
CGAAACT	20	7.857551E-4	44.0	22
ACGTTAC	20	7.857551E-4	44.0	22
ATTACCG	20	7.857551E-4	44.0	34
CCAATTA	20	7.857551E-4	44.0	34
TAACGGC	50	2.7284841E-11	44.0	25
TAACGCG	20	7.857551E-4	44.0	20
GTTACGT	25	4.4437445E-5	44.0	39
CCGTATA	45	4.802132E-10	44.0	17
CTAGTAG	20	7.857551E-4	44.0	13
TCGTCAA	35	1.4466423E-7	44.0	20
AATCTCG	30	2.528437E-6	44.0	27
AACGATA	20	7.857551E-4	44.0	44
AAACACG	25	4.4437445E-5	44.0	42
AACGAAC	45	4.802132E-10	44.0	12
>>END_MODULE