FastQCFastQC Report
Fri 17 Jun 2016
SRR1527197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835351
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC75990.41403524448457No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC71220.3880456653795378No Hit
TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG69430.3782927625288024No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA55160.3005419671768506No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC53250.29013523843668054No Hit
TACTTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC50370.274443417090246No Hit
TACTTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG49160.267850672705112No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT48030.2616938122462679No Hit
TACTTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT45890.25003391721801443No Hit
TACTTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT33430.1821449956983705No Hit
TACTTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT33420.18209051020758427No Hit
TACTTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG31960.17413562855279455No Hit
TACTTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC30850.16808773907552288No Hit
TACTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC30360.16541795002699755No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAG29760.1621488205798237No Hit
TACTTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG28960.1577899813169252No Hit
TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT28420.15484776481446874No Hit
TACTTCGGGATTCAAATGACTGGAGATCTGGGAGATTTTTGGAGTGCTTCCA25920.14122639211791096No Hit
TACTTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG25390.1383386611062407No Hit
TACTTCGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT23430.12765950491213943No Hit
TACTTCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT23050.12558905626226263No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22320.12161161543486776No Hit
TACTTCGGGATTCTATGAAAGTTGAGTTAAATGATAGCTAAAATATCTGTTT21530.11730726166275553No Hit
TACTTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT21170.11534578399445121No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG21090.11490990006816135No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT20790.11327533534457443No Hit
TACTTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG20390.11109591571312517No Hit
TACTTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG19890.10837164117381362No Hit
TACTTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT19860.10820818470145493No Hit
TACTTCGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCG19850.10815369921066868No Hit
TACTTCGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACG19010.10357691798462529No Hit
TACTTCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA18440.10047124500981013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA405.6152203E-946.00000419
ACACGCG206.3124433E-446.00000412
CGTCTAT206.3124433E-446.00000423
GATCAAT206.3124433E-446.00000413
TAACGCC206.3124433E-446.00000420
CGTTAAC206.3124433E-446.00000443
CCGTATA206.3124433E-446.00000417
CCGTACT206.3124433E-446.00000419
CGTAGGG206.3124433E-446.00000414
GTACTAG206.3124433E-446.00000433
CATACGT206.3124433E-446.00000431
CTACCGA206.3124433E-446.00000413
TCATACG206.3124433E-446.00000430
CAATCGC206.3124433E-446.00000432
ACGACAC206.3124433E-446.00000426
CAAACCG453.110472E-1046.013
TTTACGC600.046.013
GCGAATA253.4179597E-546.029
TTTCGAA301.8620158E-646.027
GACCGTA253.4179597E-546.032
Produced by FastQC (version 0.11.5)