##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527197_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1835351
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5165796624188	34.0	31.0	34.0	31.0	34.0
2	32.847686355362	34.0	31.0	34.0	31.0	34.0
3	32.980450061050995	34.0	31.0	34.0	31.0	34.0
4	36.405612332463924	37.0	37.0	37.0	35.0	37.0
5	36.4380219369483	37.0	37.0	37.0	35.0	37.0
6	36.282439707718034	37.0	37.0	37.0	35.0	37.0
7	36.5107099404964	37.0	37.0	37.0	35.0	37.0
8	36.60835011940495	37.0	37.0	37.0	35.0	37.0
9	38.639136056263894	39.0	39.0	39.0	38.0	39.0
10	38.05126757770039	39.0	38.0	39.0	37.0	39.0
11	37.789468063602	39.0	38.0	39.0	35.0	39.0
12	37.43964887370318	39.0	37.0	39.0	35.0	39.0
13	37.36650210232266	39.0	37.0	39.0	35.0	39.0
14	38.20456305088236	40.0	38.0	40.0	35.0	40.0
15	38.0760497583296	40.0	38.0	40.0	34.0	40.0
16	33.012951201159886	40.0	35.0	40.0	3.0	40.0
17	34.715375968956344	40.0	35.0	40.0	17.0	40.0
18	36.755909360116945	40.0	35.0	40.0	30.0	40.0
19	37.597067808827845	40.0	37.0	40.0	33.0	40.0
20	37.87286410065432	40.0	38.0	40.0	34.0	40.0
21	38.072552879530946	40.0	38.0	40.0	34.0	40.0
22	38.09830217762161	40.0	38.0	40.0	34.0	40.0
23	38.007374611177916	40.0	38.0	40.0	34.0	40.0
24	37.940849461492654	40.0	37.0	40.0	34.0	40.0
25	37.83505117004867	40.0	37.0	40.0	34.0	40.0
26	37.71899816438381	40.0	37.0	40.0	33.0	40.0
27	37.59889252791428	40.0	37.0	40.0	33.0	40.0
28	37.47126952828096	40.0	36.0	40.0	33.0	40.0
29	37.2316063793792	40.0	36.0	40.0	33.0	40.0
30	37.1887301121148	40.0	36.0	40.0	32.0	40.0
31	37.116542830227026	39.0	35.0	40.0	32.0	40.0
32	36.97824939207813	39.0	35.0	40.0	32.0	40.0
33	36.57212435114591	39.0	35.0	40.0	31.0	40.0
34	36.43214186278265	39.0	35.0	40.0	31.0	40.0
35	36.302743181004615	39.0	35.0	40.0	30.0	40.0
36	36.16150861606309	38.0	35.0	40.0	30.0	40.0
37	35.745893837200626	38.0	35.0	40.0	29.0	40.0
38	35.68277675496404	38.0	35.0	40.0	29.0	40.0
39	35.222491501625576	37.0	34.0	40.0	28.0	40.0
40	35.13232564234307	37.0	34.0	40.0	28.0	40.0
41	35.06317102287247	37.0	34.0	40.0	28.0	40.0
42	34.93950639414477	37.0	34.0	40.0	28.0	40.0
43	34.78464337339288	36.0	34.0	40.0	28.0	40.0
44	35.00198272700971	36.0	34.0	40.0	28.0	40.0
45	35.04439150876318	36.0	34.0	40.0	29.0	40.0
46	34.91679956585961	36.0	34.0	40.0	29.0	40.0
47	34.559693486423036	35.0	34.0	39.0	28.0	40.0
48	34.4336353100851	35.0	34.0	39.0	27.0	40.0
49	34.315968444183156	35.0	34.0	39.0	27.0	40.0
50	33.8223021100596	35.0	33.0	39.0	26.0	40.0
51	33.8386913456881	35.0	33.0	38.0	26.0	40.0
52	33.44892611821935	35.0	33.0	37.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	2.0
14	5.0
15	15.0
16	52.0
17	142.0
18	369.0
19	765.0
20	1333.0
21	2152.0
22	3351.0
23	5039.0
24	7218.0
25	10103.0
26	13716.0
27	18530.0
28	23311.0
29	27393.0
30	32690.0
31	41059.0
32	55680.0
33	80714.0
34	120881.0
35	172968.0
36	253244.0
37	387047.0
38	414613.0
39	162954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.48244232302159	0.3478353731792992	0.11774314558904538	0.05197915821006445
8	98.94521538386935	0.8126510950766365	0.10047124500981013	0.1416622760442008
9	94.59089841670612	3.1985707366056957	1.045685539169347	1.1648453075188343
10	53.02577000257717	30.61545175827403	6.427162978634604	9.931615260514201
11	33.05961638945357	23.23773490738284	20.61169770795886	23.090950995204732
12	27.284699221020936	17.518501910533736	30.510730644982893	24.68606822346243
13	23.123315376731753	17.39743515000673	32.32297255402373	27.156276919237794
14	24.963562828036707	18.648694445912525	29.742921108823328	26.64482161722744
15	23.838437443301036	19.070521115579528	29.32855895139404	27.7624824897254
16	28.042973796292912	26.709060010864405	23.28066947412239	21.967296718720288
17	31.01646497046069	18.17892054435364	23.97644919146256	26.82816529372311
18	30.86597604490912	19.26171070274841	23.26100021194856	26.61131304039391
19	30.37849435884471	19.45878472292221	22.093703057344342	28.069017860888735
20	28.837099824502232	21.213435468201993	23.03679241736322	26.912672289932555
21	30.938986602562668	18.938720713367633	25.54203528371412	24.580257400355574
22	31.989194437467273	18.795914242016924	22.27448591577306	26.940405404742744
23	29.83282216862061	20.82811407736177	22.65800928541734	26.681054468600284
24	30.243533798167217	19.43551941835649	23.04916062377169	27.271786159704604
25	28.11162551468357	18.693971888755883	23.710668967407326	29.483733629153225
26	29.025401680659446	18.65844734876326	23.02878305021764	29.287367920359646
27	29.123584535056235	17.30170414269532	23.527488747384016	30.047222574864424
28	29.63574814844681	19.676290802140844	22.56505703813603	28.12290401127632
29	30.505173124922695	19.952804667880965	22.12644883730687	27.41557336988947
30	30.427912698987825	20.604832536119794	21.44854036094458	27.518714403947804
31	32.86118023201012	20.734344547718667	20.005110739035747	26.39936448123547
32	33.95432263365427	19.942016540705293	21.835060432582107	24.268600393058332
33	32.669772702878085	19.703424576552386	22.116260050529842	25.510542670039683
34	30.15079949284905	22.600854005582583	23.69868215943435	23.54966434213401
35	30.690859677522177	23.317719607857025	23.542690199313373	22.448730515307428
36	31.960861982258436	23.39863056167458	20.365150862151165	24.275356593915824
37	32.05032715812943	24.16496898958292	20.62401142887655	23.16069242341111
38	32.87910595847879	22.948961806215813	20.252910751131527	23.91902148417387
39	31.182318804413978	22.564948067154457	20.928530836880793	25.324202291550773
40	29.482262520901998	23.284211031023496	22.275412169116425	24.958114278958085
41	27.720692118292362	22.738756782762533	21.987129437366477	27.553421661578632
42	27.557834986332313	21.880882730333326	23.636950098373553	26.924332184960807
43	26.00979322211392	21.08446831151099	25.116285658710513	27.78945280766458
44	26.698380854670305	20.57175984321255	25.071662041756593	27.658197260360552
45	27.269061885165293	21.702660689971566	24.21596740895883	26.812310015904316
46	27.84023328507735	21.8754341812547	22.83535955792652	27.448972975741427
47	28.32962196331928	22.22474066268523	23.25391709814635	26.191720275849146
48	28.565271710969725	23.021373023470716	23.79403176831026	24.6193234972493
49	27.90354542537095	22.63501640830555	24.004073335291178	25.45736483103232
50	25.725760358645296	24.081170304753698	23.378743357537605	26.8143259790634
51	26.18954085621769	24.839008996099384	22.789591745666087	26.181858402016832
52	28.45003489795685	23.52972265250625	22.813292934158095	25.206949515378803
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	11.0
4	22.0
5	120.5
6	219.0
7	282.0
8	345.0
9	1715.5
10	3086.0
11	2833.0
12	2580.0
13	2624.0
14	2549.0
15	2430.0
16	2497.0
17	2564.0
18	3343.5
19	4123.0
20	4347.0
21	4571.0
22	5702.0
23	6833.0
24	7112.0
25	7391.0
26	9607.5
27	11824.0
28	14297.0
29	16770.0
30	18362.5
31	19955.0
32	19477.5
33	19000.0
34	21560.0
35	24120.0
36	25072.0
37	26024.0
38	27356.0
39	31534.5
40	34381.0
41	37453.5
42	40526.0
43	42855.5
44	45185.0
45	44036.5
46	42888.0
47	45659.5
48	48431.0
49	58431.0
50	68431.0
51	81485.5
52	94540.0
53	104576.0
54	114612.0
55	135504.0
56	156396.0
57	175112.0
58	193828.0
59	181023.5
60	168219.0
61	170063.5
62	171908.0
63	153018.0
64	124089.5
65	114051.0
66	92025.0
67	69999.0
68	62933.5
69	55868.0
70	47821.0
71	39774.0
72	37996.0
73	36218.0
74	26160.0
75	16102.0
76	10157.5
77	4213.0
78	2756.5
79	1300.0
80	1161.5
81	1023.0
82	542.0
83	61.0
84	48.0
85	35.0
86	23.5
87	12.0
88	8.5
89	3.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1835351.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.855021788713454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.97747436411359	7.81317052519017
2	9.835554167017666	2.135303095740963
3	4.275414705075548	1.3922915933814293
4	2.3552472027003697	1.0226503881247568
5	1.405959355081803	0.7630859716729244
6	0.903875628524573	0.5886953785152785
7	0.6126402916983058	0.46551566005202133
8	0.4478394502510348	0.388904559225635
9	0.34041941500715883	0.3325734150483417
>10	3.53545799120214	9.380054401903555
>50	2.068786651278646	16.449282441165746
>100	2.101320224003049	41.158805150365225
>500	0.09121019495949094	6.76583703075594
>1k	0.045314619151849	8.976320310203192
>5k	0.0034857399347576153	2.3675100786548184
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	7599	0.41403524448457	No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	7122	0.3880456653795378	No Hit
TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6943	0.3782927625288024	No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5516	0.3005419671768506	No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5325	0.29013523843668054	No Hit
TACTTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	5037	0.274443417090246	No Hit
TACTTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	4916	0.267850672705112	No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4803	0.2616938122462679	No Hit
TACTTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4589	0.25003391721801443	No Hit
TACTTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3343	0.1821449956983705	No Hit
TACTTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3342	0.18209051020758427	No Hit
TACTTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3196	0.17413562855279455	No Hit
TACTTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	3085	0.16808773907552288	No Hit
TACTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3036	0.16541795002699755	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAG	2976	0.1621488205798237	No Hit
TACTTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	2896	0.1577899813169252	No Hit
TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2842	0.15484776481446874	No Hit
TACTTCGGGATTCAAATGACTGGAGATCTGGGAGATTTTTGGAGTGCTTCCA	2592	0.14122639211791096	No Hit
TACTTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2539	0.1383386611062407	No Hit
TACTTCGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT	2343	0.12765950491213943	No Hit
TACTTCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	2305	0.12558905626226263	No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2232	0.12161161543486776	No Hit
TACTTCGGGATTCTATGAAAGTTGAGTTAAATGATAGCTAAAATATCTGTTT	2153	0.11730726166275553	No Hit
TACTTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	2117	0.11534578399445121	No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2109	0.11490990006816135	No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2079	0.11327533534457443	No Hit
TACTTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	2039	0.11109591571312517	No Hit
TACTTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1989	0.10837164117381362	No Hit
TACTTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1986	0.10820818470145493	No Hit
TACTTCGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCG	1985	0.10815369921066868	No Hit
TACTTCGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACG	1901	0.10357691798462529	No Hit
TACTTCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	1844	0.10047124500981013	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.6345647235869323E-4	0.0	0.0	0.0	0.0
8	0.002070448649876781	0.0	0.0	0.0	0.0
9	0.0036505278826774825	0.0	0.0	0.0	0.0
10	0.019560291192256957	0.0	0.0	0.0	0.0
11	0.03465277214004297	0.0	0.0	0.0	0.0
12	0.04625818167751019	0.0	0.0	0.0	0.0
13	0.05737322179790133	0.0	0.0	0.0	0.0
14	0.07813219378745537	0.0	0.0	0.0	0.0
15	0.09780145596128478	0.0	0.0	0.0	0.0
16	0.12030396365599823	0.0	0.0	0.0	0.0
17	0.13403430733412847	0.0	0.0	0.0	0.0
18	0.14645699923338915	0.0	0.0	0.0	0.0
19	0.15909763309579475	0.0	0.0	0.0	0.0
20	0.1754432803316641	0.0	0.0	0.0	0.0
21	0.18901016753743563	0.0	0.0	0.0	0.0
22	0.20268602572477962	0.0	0.0	0.0	0.0
23	0.21478180467932292	0.0	0.0	0.0	0.0
24	0.2285666338482394	0.0	0.0	0.0	0.0
25	0.24186109360007976	0.0	0.0	0.0	0.0
26	0.2568446035662933	0.0	0.0	0.0	0.0
27	0.27422547512710105	0.0	0.0	0.0	0.0
28	0.30201307542807887	0.0	0.0	0.0	0.0
29	0.3141088543826222	0.0	0.0	0.0	0.0
30	0.3267494882450278	0.0	0.0	0.0	0.0
31	0.33966254956136455	0.0	0.0	0.0	0.0
32	0.35181281400669406	0.0	0.0	0.0	0.0
33	0.36309131059944394	0.0	0.0	0.0	0.0
34	0.37894658841823714	0.0	0.0	0.0	0.0
35	0.41768577236724747	0.0	0.0	0.0	0.0
36	0.43217891291638494	0.0	0.0	0.0	0.0
37	0.444928517760363	0.0	0.0	0.0	5.4485490786231084E-5
38	0.4589312888924244	0.0	0.0	0.0	5.4485490786231084E-5
39	0.47326097296920316	0.0	0.0	0.0	5.4485490786231084E-5
40	0.4873182295920508	0.0	0.0	0.0	5.4485490786231084E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTTA	40	5.6152203E-9	46.000004	19
ACACGCG	20	6.3124433E-4	46.000004	12
CGTCTAT	20	6.3124433E-4	46.000004	23
GATCAAT	20	6.3124433E-4	46.000004	13
TAACGCC	20	6.3124433E-4	46.000004	20
CGTTAAC	20	6.3124433E-4	46.000004	43
CCGTATA	20	6.3124433E-4	46.000004	17
CCGTACT	20	6.3124433E-4	46.000004	19
CGTAGGG	20	6.3124433E-4	46.000004	14
GTACTAG	20	6.3124433E-4	46.000004	33
CATACGT	20	6.3124433E-4	46.000004	31
CTACCGA	20	6.3124433E-4	46.000004	13
TCATACG	20	6.3124433E-4	46.000004	30
CAATCGC	20	6.3124433E-4	46.000004	32
ACGACAC	20	6.3124433E-4	46.000004	26
CAAACCG	45	3.110472E-10	46.0	13
TTTACGC	60	0.0	46.0	13
GCGAATA	25	3.4179597E-5	46.0	29
TTTCGAA	30	1.8620158E-6	46.0	27
GACCGTA	25	3.4179597E-5	46.0	32
>>END_MODULE