FastQCFastQC Report
Fri 17 Jun 2016
SRR1527196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782154
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC75460.4234201982544718No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC71170.3993482044761564No Hit
TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG66180.37134837954520206No Hit
TACTTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC54100.30356523622537673No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA53760.3016574325226664No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC50450.2830844023580454No Hit
TACTTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT48830.27399427883336686No Hit
TACTTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG47050.26400636533094224No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT46340.2600224223046942No Hit
TACTTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT34300.1924637264793054No Hit
TACTTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG33800.18965813279884905No Hit
TACTTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT33110.18578641351981928No Hit
TACTTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC31300.17563016439656728No Hit
TACTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC30360.17035564827730937No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAG29730.16682060023993436No Hit
TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT26590.14920147192666852No Hit
TACTTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG25600.14364639643936494No Hit
TACTTCGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT23920.13421960167303162No Hit
TACTTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG23750.13326569982167646No Hit
TACTTCGGGATTCAAATGACTGGAGATCTGGGAGATTTTTGGAGTGCTTCCA23180.13006732302595622No Hit
TACTTCGGGATTCTATGAAAGTTGAGTTAAATGATAGCTAAAATATCTGTTT21860.12266055570955146No Hit
TACTTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG20950.11755437521112093No Hit
TACTTCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT20870.11710548022224791No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT20500.11502934089871021No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG20480.11491711715149194No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19820.11121373349328958No Hit
TACTTCGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCG19530.1095864891586249No Hit
TACTTCGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACG19190.10767868545591458No Hit
TACTTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT19080.1070614548462142No Hit
TACTTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG18920.10616366486846816No Hit
TACTTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT18600.1043680849129761No Hit
TACTTCGGAGAATTTTTTTAACTCTAGCACAAAGCTCTTGCTCATGAAATTC18480.10369474242966659No Hit
TACTTCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA17980.10088914874921023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA501.6370905E-1146.019
CAAACGT253.4179193E-546.044
TAACGGG206.3123956E-446.045
CGTTATA301.8619849E-646.046
CCGTACT206.3123956E-446.019
TATTACG206.3123956E-446.030
ACGTCGT501.6370905E-1146.044
TTACGTA206.3123956E-446.032
ACCGTAT206.3123956E-446.019
AATAGAC551.8189894E-1246.026
TGTACGA253.4179193E-546.035
ACATACG253.4179193E-546.038
CGCCCAA253.4179193E-546.015
CCCGAAA301.8619849E-646.018
AACGTCT351.0199983E-745.99999639
TAGCTAA2850.044.38596735
TACGTCA2100.043.80952525
CGGGACT41950.043.6972586
CCTAATA1500.042.93333413
GTATATC750.042.93333434
Produced by FastQC (version 0.11.5)