##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527196_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1782154
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.491965901936645	34.0	31.0	34.0	31.0	34.0
2	32.842882826063295	34.0	31.0	34.0	31.0	34.0
3	32.97017822253296	34.0	31.0	34.0	31.0	34.0
4	36.40701757536105	37.0	37.0	37.0	35.0	37.0
5	36.44681548283706	37.0	37.0	37.0	35.0	37.0
6	36.28049091155983	37.0	37.0	37.0	35.0	37.0
7	36.51375021462792	37.0	37.0	37.0	35.0	37.0
8	36.63371627816676	37.0	37.0	37.0	35.0	37.0
9	38.58005424895941	39.0	39.0	39.0	38.0	39.0
10	38.045773260896645	39.0	38.0	39.0	37.0	39.0
11	37.71572602592144	39.0	38.0	39.0	35.0	39.0
12	37.39685347057549	39.0	37.0	39.0	35.0	39.0
13	37.26220910201924	39.0	37.0	39.0	34.0	39.0
14	38.12367393614693	40.0	38.0	40.0	34.0	40.0
15	38.19110357466302	40.0	38.0	40.0	34.0	40.0
16	38.257313341046846	40.0	38.0	40.0	35.0	40.0
17	38.237229779244664	40.0	38.0	40.0	35.0	40.0
18	38.211326293911746	40.0	38.0	40.0	34.0	40.0
19	38.12767359049779	40.0	38.0	40.0	34.0	40.0
20	38.1002904350578	40.0	38.0	40.0	34.0	40.0
21	33.777958021585114	40.0	34.0	40.0	10.0	40.0
22	34.50331677284903	40.0	34.0	40.0	16.0	40.0
23	36.52145325263698	40.0	34.0	40.0	30.0	40.0
24	37.28958215732198	39.0	36.0	40.0	32.0	40.0
25	37.526565605441505	39.0	37.0	40.0	33.0	40.0
26	37.58919823988275	40.0	37.0	40.0	33.0	40.0
27	37.547593530076526	40.0	37.0	40.0	33.0	40.0
28	37.42826321406567	40.0	36.0	40.0	33.0	40.0
29	37.37322083276754	40.0	36.0	40.0	33.0	40.0
30	37.239024798081424	40.0	36.0	40.0	33.0	40.0
31	37.067946989990766	40.0	35.0	40.0	32.0	40.0
32	36.963044720041026	39.0	35.0	40.0	32.0	40.0
33	36.581666904206934	39.0	35.0	40.0	31.0	40.0
34	36.42460752550004	39.0	35.0	40.0	31.0	40.0
35	36.30053912288164	39.0	35.0	40.0	31.0	40.0
36	36.09888988269252	38.0	35.0	40.0	30.0	40.0
37	35.8926153407618	38.0	35.0	40.0	30.0	40.0
38	35.759156616094906	38.0	35.0	40.0	30.0	40.0
39	35.51008835375619	38.0	34.0	40.0	29.0	40.0
40	30.671039652016603	35.0	29.0	40.0	8.0	40.0
41	31.480746893927236	35.0	28.0	40.0	12.0	40.0
42	33.17665476720867	35.0	30.0	39.0	23.0	40.0
43	33.99127965372241	35.0	33.0	39.0	26.0	40.0
44	34.6829067521662	36.0	33.0	39.0	28.0	40.0
45	34.92290060230485	36.0	34.0	40.0	29.0	40.0
46	34.88977102988855	36.0	34.0	40.0	29.0	40.0
47	34.77462553741147	35.0	34.0	39.0	29.0	40.0
48	34.55457496939097	35.0	34.0	39.0	28.0	40.0
49	34.37868837373201	35.0	34.0	39.0	28.0	40.0
50	34.26134049021577	35.0	34.0	39.0	28.0	40.0
51	34.03116453460251	35.0	34.0	39.0	27.0	40.0
52	33.581587225346404	35.0	33.0	37.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	3.0
14	7.0
15	14.0
16	66.0
17	188.0
18	420.0
19	801.0
20	1444.0
21	2358.0
22	3541.0
23	5270.0
24	7545.0
25	10621.0
26	14115.0
27	18764.0
28	24129.0
29	28636.0
30	35158.0
31	45895.0
32	64033.0
33	84197.0
34	140366.0
35	192118.0
36	235758.0
37	299064.0
38	401743.0
39	165896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.50161433860374	0.3422824290156743	0.10930592979057926	0.046797302590011865
8	98.96535316252131	0.8115460280088029	0.08725396346219237	0.1358468460076963
9	94.47578604318146	3.2290699905844273	1.0893559142475904	1.2057880519865287
10	53.01264649407402	30.597860790930525	6.467791223429625	9.921701491565823
11	33.207792368111846	23.261962770894097	20.489867878982402	23.040376982011658
12	27.35145223140088	17.551345169946032	30.4741341096224	24.62306848903069
13	23.295686006933185	17.377847256746612	32.25400274050391	27.0724639958163
14	24.932413248237808	18.687778946151678	29.786931993531425	26.592875812079093
15	23.871281606415607	19.008289968207013	29.26784105077339	27.852587374603992
16	29.382477608556833	18.58301807812344	26.490303307121604	25.54420100619812
17	30.98396659323493	18.225473219486084	24.02912430687808	26.761435880400906
18	30.919830721699697	19.301250060320264	23.175494373662435	26.60342484431761
19	30.371561604664915	19.48512867013737	22.021722028511565	28.121587696686145
20	28.8191143975212	21.28222364621688	22.92557208860738	26.973089867654537
21	35.254472958004754	18.561078335542273	24.211992902970223	21.972455803482752
22	32.0885288252306	18.75578653696594	22.285952841336947	26.869731796466525
23	29.77335292011801	20.865536872795502	22.601413794767456	26.759696412319023
24	30.31213913051285	19.42228337169515	23.083975907805947	27.181601589986048
25	28.219502916134072	18.610288448697474	23.73027246803587	29.43993616713258
26	29.03604290089409	18.62611199705525	23.052721594205664	29.285123507845
27	29.15516840856626	17.310849679657313	23.538874867155137	29.99510704462128
28	29.598003315089493	19.71142785640298	22.57683679412666	28.113732034380867
29	30.38732904114908	19.974087536767303	22.186803160669616	27.451780261414
30	30.46975738348089	20.532007896062854	21.463857781089626	27.534376939366634
31	32.90899664114325	20.73788236033474	20.023578209290555	26.329542789231457
32	33.980284532088696	19.91146668581952	21.92997911516064	24.17826966693114
33	32.62181607201173	19.709856723941925	22.19443437548046	25.47389282856588
34	30.06238518107863	22.62621524290269	23.75917008294457	23.552229493074112
35	30.639888584263765	23.37687988804559	23.51699123644758	22.46624029124307
36	31.99532700316583	23.412398704040164	20.388473723370705	24.203800569423294
37	31.934052837184666	24.17759632444783	20.6576984929473	23.230652345420204
38	32.849405831370355	23.006485410351743	20.226254296766722	23.917854461511183
39	30.9752131409519	22.588788623205403	20.92838217123773	25.507616064604967
40	29.653722405583355	27.631169921342373	20.538629097148732	22.176478575925536
41	27.729870707020833	22.744330736849903	22.039341156824833	27.486457399304438
42	27.51793616039916	21.89664866223682	23.651996404351138	26.933418773012885
43	25.863140895792398	21.186160118598053	25.125606429074033	27.82509255653552
44	26.600226467521885	20.59973492750907	25.123866960992146	27.676171643976893
45	27.193665642812014	21.803166280804014	24.266701979739125	26.736466096644847
46	27.863360854336943	21.994227210443093	22.701854048527792	27.440557886692176
47	28.107615840157475	22.258794694510126	23.240640258922628	26.39294920640977
48	28.443389291834485	23.136216062136043	23.822576500122885	24.597818145906583
49	27.772459619090156	22.834390293992552	23.996130525195912	25.39701956172138
50	25.669442708093687	24.134334069895193	23.464470522749437	26.73175269926168
51	26.186906406517057	24.894649957298864	22.76290376701452	26.15553986916955
52	28.42846353345446	23.612998652192797	22.822606800534633	25.13593101381811
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	9.0
4	18.0
5	116.0
6	214.0
7	269.0
8	324.0
9	1547.0
10	2770.0
11	2626.0
12	2482.0
13	2588.0
14	2520.5
15	2347.0
16	2392.0
17	2437.0
18	3163.5
19	3890.0
20	4041.0
21	4192.0
22	5353.5
23	6515.0
24	6785.5
25	7056.0
26	9205.5
27	11355.0
28	13699.0
29	16043.0
30	17535.5
31	19028.0
32	18907.0
33	18786.0
34	21099.5
35	23413.0
36	24340.0
37	25267.0
38	26534.5
39	30712.5
40	33623.0
41	36297.5
42	38972.0
43	41498.5
44	44025.0
45	43224.0
46	42423.0
47	44873.0
48	47323.0
49	56450.5
50	65578.0
51	78649.5
52	91721.0
53	100525.0
54	109329.0
55	129741.0
56	150153.0
57	169769.5
58	189386.0
59	176141.5
60	162897.0
61	165245.0
62	167593.0
63	149108.5
64	120982.5
65	111341.0
66	89742.5
67	68144.0
68	61731.0
69	55318.0
70	46718.5
71	38119.0
72	37049.0
73	35979.0
74	26183.0
75	16387.0
76	10345.0
77	4303.0
78	2759.5
79	1216.0
80	1088.0
81	960.0
82	521.0
83	82.0
84	45.5
85	9.0
86	8.0
87	7.0
88	6.5
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1782154.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.151789093800222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.55188334459469	8.09083301416917
2	9.543499000522067	2.128541761414306
3	4.135168694992072	1.383435874615101
4	2.314507167182498	1.0324358313803292
5	1.387140362849651	0.7734548384998412
6	0.8943010976528365	0.5983834336427082
7	0.594329933874676	0.46394894522918334
8	0.46089473727351715	0.4111840723613382
9	0.3390193951816671	0.3402605514396297
>10	3.5355072848394284	9.745273172691931
>50	2.0851943202356726	16.954022834222172
>100	2.0259191650586943	40.382350621347534
>500	0.08509799505605756	6.442235470306397
>1k	0.044016204339340115	8.824736235553456
>5k	0.0035212963471472087	2.4289033431269087
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	7546	0.4234201982544718	No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	7117	0.3993482044761564	No Hit
TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6618	0.37134837954520206	No Hit
TACTTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	5410	0.30356523622537673	No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5376	0.3016574325226664	No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5045	0.2830844023580454	No Hit
TACTTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4883	0.27399427883336686	No Hit
TACTTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	4705	0.26400636533094224	No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4634	0.2600224223046942	No Hit
TACTTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3430	0.1924637264793054	No Hit
TACTTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3380	0.18965813279884905	No Hit
TACTTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3311	0.18578641351981928	No Hit
TACTTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	3130	0.17563016439656728	No Hit
TACTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3036	0.17035564827730937	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAG	2973	0.16682060023993436	No Hit
TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2659	0.14920147192666852	No Hit
TACTTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	2560	0.14364639643936494	No Hit
TACTTCGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT	2392	0.13421960167303162	No Hit
TACTTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2375	0.13326569982167646	No Hit
TACTTCGGGATTCAAATGACTGGAGATCTGGGAGATTTTTGGAGTGCTTCCA	2318	0.13006732302595622	No Hit
TACTTCGGGATTCTATGAAAGTTGAGTTAAATGATAGCTAAAATATCTGTTT	2186	0.12266055570955146	No Hit
TACTTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	2095	0.11755437521112093	No Hit
TACTTCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	2087	0.11710548022224791	No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2050	0.11502934089871021	No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2048	0.11491711715149194	No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1982	0.11121373349328958	No Hit
TACTTCGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCG	1953	0.1095864891586249	No Hit
TACTTCGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACG	1919	0.10767868545591458	No Hit
TACTTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1908	0.1070614548462142	No Hit
TACTTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1892	0.10616366486846816	No Hit
TACTTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	1860	0.1043680849129761	No Hit
TACTTCGGAGAATTTTTTTAACTCTAGCACAAAGCTCTTGCTCATGAAATTC	1848	0.10369474242966659	No Hit
TACTTCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	1798	0.10088914874921023	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.683356208273808E-4	0.0	0.0	0.0	0.0
8	0.0014589087138373003	0.0	0.0	0.0	0.0
9	0.0023005868179742043	0.0	0.0	0.0	0.0
10	0.019470820142367045	0.0	0.0	0.0	0.0
11	0.035518815994577346	0.0	0.0	0.0	0.0
12	0.049154001281595194	0.0	0.0	0.0	0.0
13	0.06071304724507534	0.0	0.0	0.0	0.0
14	0.0797910842721785	0.0	0.0	0.0	0.0
15	0.09898134504649991	0.0	0.0	0.0	0.0
16	0.11789104645277569	0.0	0.0	0.0	0.0
17	0.1306284417620475	0.0	0.0	0.0	0.0
18	0.14201915210470026	0.0	0.0	0.0	0.0
19	0.15531766615006334	0.0	0.0	0.0	0.0
20	0.16923341080512683	0.0	0.0	0.0	0.0
21	0.18124135175748	0.0	0.0	0.0	0.0
22	0.19554987952780736	0.0	0.0	0.0	0.0
23	0.2087361698259522	0.0	0.0	0.0	0.0
24	0.22411082319485298	0.0	0.0	0.0	0.0
25	0.23712877787217043	0.0	0.0	0.0	0.0
26	0.2531206618507716	0.0	0.0	0.0	0.0
27	0.2713009089001287	0.0	0.0	0.0	0.0
28	0.29694403513949974	0.0	0.0	0.0	0.0
29	0.30827863360854335	0.0	0.0	0.0	0.0
30	0.3204549101817239	0.0	0.0	0.0	0.0
31	0.33201395614520407	0.0	0.0	0.0	0.0
32	0.34463912770725763	0.0	0.0	0.0	0.0
33	0.35372925123193616	0.0	0.0	0.0	0.0
34	0.369103904600837	0.0	0.0	0.0	0.0
35	0.40451049684819607	0.0	0.0	0.0	0.0
36	0.4179773465143865	0.0	0.0	0.0	0.0
37	0.4304341824556127	0.0	0.0	0.0	5.611187360912693E-5
38	0.4444621508578944	0.0	0.0	0.0	5.611187360912693E-5
39	0.4574801055352119	0.0	0.0	0.0	5.611187360912693E-5
40	0.4729108707777218	0.0	0.0	0.0	5.611187360912693E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTTA	50	1.6370905E-11	46.0	19
CAAACGT	25	3.4179193E-5	46.0	44
TAACGGG	20	6.3123956E-4	46.0	45
CGTTATA	30	1.8619849E-6	46.0	46
CCGTACT	20	6.3123956E-4	46.0	19
TATTACG	20	6.3123956E-4	46.0	30
ACGTCGT	50	1.6370905E-11	46.0	44
TTACGTA	20	6.3123956E-4	46.0	32
ACCGTAT	20	6.3123956E-4	46.0	19
AATAGAC	55	1.8189894E-12	46.0	26
TGTACGA	25	3.4179193E-5	46.0	35
ACATACG	25	3.4179193E-5	46.0	38
CGCCCAA	25	3.4179193E-5	46.0	15
CCCGAAA	30	1.8619849E-6	46.0	18
AACGTCT	35	1.0199983E-7	45.999996	39
TAGCTAA	285	0.0	44.385967	35
TACGTCA	210	0.0	43.809525	25
CGGGACT	4195	0.0	43.697258	6
CCTAATA	150	0.0	42.933334	13
GTATATC	75	0.0	42.933334	34
>>END_MODULE