FastQCFastQC Report
Fri 17 Jun 2016
SRR1527195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906362
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT106130.5567148317056257No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC58420.30644756871989687No Hit
ACAATAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA58370.3061852890479353No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC52700.27644277424749336No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC45780.2401432676480123No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA44280.2322748774891652No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC42400.22241316182341025No Hit
ACAATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG40290.21134495966663203No Hit
ACAATAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC31390.16465917805747282No Hit
ACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC28950.15185993006574827No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG28180.1478208231175401No Hit
ACAATAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG26410.13853612273010057No Hit
ACAATAGGGCAGTAGATTTCATTAAGAAAAATTGTGGACAAACTTGTTTC25990.1363329734856234No Hit
ACAATAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT25500.13376263270040004No Hit
ACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG25170.1320315868654537No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT24500.12851703926116867No Hit
ACAATAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA24310.1275203765077147No Hit
ACAATAGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATG23920.12547459506641448No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG23650.124058284837822No Hit
ACAATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG23330.12237969493726795No Hit
ACAATAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC23050.12091092877428317No Hit
ACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA22990.12059619316792927No Hit
ACAATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC22930.1202814575615754No Hit
ACAATAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT22060.11571779126944409No Hit
ACAATAGGGAGAAAATGAAGTTTTAGGACTTTCATGGTAACAAACCTGAC21250.11146886058366669No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC20570.10790185704498936No Hit
ACAATAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA20170.10580361966929681No Hit
ACAATAGGGAGAGGACTTGTAGTTTATTCATAAATCTTCATGTTATTGTA19720.10344310262164269No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC19360.1015546889835194No Hit
ACAATAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC19350.10150223304912709No Hit
ACAATAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG19230.10087276183641933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA207.857788E-444.019
ACCGATA207.857788E-444.031
TATGCGC408.3182385E-944.023
TTATAGG454.802132E-1044.029
AATTCGC302.528599E-644.041
TCGTACG207.857788E-444.036
CGTAAGG254.4439475E-544.020
CGATATT207.857788E-444.044
CGATACC254.4439475E-544.036
GCGTAAG207.857788E-444.037
GTACGTA207.857788E-444.039
CCGTAAT351.4467514E-743.99999628
TAGGGAT169750.042.98915
TAGGGGT186550.042.9504175
ATAGGGG1119450.042.8463974
TAGGGGA301900.042.8267635
CAATAGG1940450.042.7823452
AATAGGG1914000.042.7781563
ACAATAG1956950.042.767881
TAGGGGC289200.042.7524225
Produced by FastQC (version 0.11.5)