##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527195_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1906362
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.515708454113124	34.0	31.0	34.0	31.0	34.0
2	32.94309737604925	34.0	31.0	34.0	31.0	34.0
3	33.02451160902284	34.0	31.0	34.0	31.0	34.0
4	36.50710725455082	37.0	37.0	37.0	35.0	37.0
5	36.31148438754025	37.0	37.0	37.0	35.0	37.0
6	36.327408435543724	37.0	37.0	37.0	35.0	37.0
7	36.449295569257046	37.0	37.0	37.0	35.0	37.0
8	36.63578848088663	37.0	37.0	37.0	35.0	37.0
9	38.75340622609976	39.0	39.0	39.0	39.0	39.0
10	38.310348716560654	39.0	39.0	39.0	37.0	39.0
11	38.0126507976974	39.0	38.0	39.0	35.0	39.0
12	37.69146206229457	39.0	37.0	39.0	35.0	39.0
13	37.596744479799746	39.0	37.0	39.0	35.0	39.0
14	38.41667217453978	40.0	38.0	40.0	35.0	40.0
15	38.43240003734863	40.0	38.0	40.0	35.0	40.0
16	38.33491120783985	40.0	38.0	40.0	35.0	40.0
17	38.4157636377561	40.0	38.0	40.0	35.0	40.0
18	38.40181193288578	40.0	38.0	40.0	35.0	40.0
19	38.363186005596	40.0	38.0	40.0	35.0	40.0
20	38.1286308686388	40.0	38.0	40.0	34.0	40.0
21	38.248953766388546	40.0	38.0	40.0	35.0	40.0
22	38.05630567541736	40.0	38.0	40.0	34.0	40.0
23	38.11591659926079	40.0	38.0	40.0	34.0	40.0
24	38.12617855370596	40.0	38.0	40.0	34.0	40.0
25	38.07680178266247	40.0	38.0	40.0	34.0	40.0
26	37.94491025314185	40.0	38.0	40.0	34.0	40.0
27	37.81057689987526	40.0	38.0	40.0	34.0	40.0
28	37.601520592626166	40.0	37.0	40.0	33.0	40.0
29	37.858815377142434	40.0	38.0	40.0	34.0	40.0
30	37.96206806472223	40.0	38.0	40.0	34.0	40.0
31	37.91253759779097	40.0	38.0	40.0	34.0	40.0
32	37.2066559236913	40.0	37.0	40.0	33.0	40.0
33	37.42017623095718	40.0	37.0	40.0	33.0	40.0
34	37.411473266882155	40.0	37.0	40.0	33.0	40.0
35	37.44178020753666	40.0	37.0	40.0	33.0	40.0
36	37.25357670788654	40.0	36.0	40.0	33.0	40.0
37	37.16337558134289	40.0	36.0	40.0	32.0	40.0
38	36.95032842660523	40.0	35.0	40.0	32.0	40.0
39	36.90095742571453	40.0	35.0	40.0	32.0	40.0
40	36.77935407860627	39.0	35.0	40.0	31.0	40.0
41	36.66969966879323	39.0	35.0	40.0	31.0	40.0
42	36.55269775624986	39.0	35.0	40.0	31.0	40.0
43	36.422910234257714	39.0	35.0	40.0	31.0	40.0
44	36.29835519172119	39.0	35.0	40.0	31.0	40.0
45	36.17438555741249	39.0	35.0	40.0	30.0	40.0
46	35.86455930195839	38.0	35.0	40.0	30.0	40.0
47	35.75849550085451	38.0	35.0	40.0	29.0	40.0
48	35.61294287234009	38.0	35.0	40.0	29.0	40.0
49	35.2659930275572	37.0	34.0	40.0	28.0	40.0
50	34.95260134224245	37.0	34.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	1.0
15	17.0
16	37.0
17	95.0
18	190.0
19	392.0
20	743.0
21	1187.0
22	1884.0
23	2685.0
24	4005.0
25	5888.0
26	8192.0
27	11099.0
28	15028.0
29	19884.0
30	25941.0
31	33431.0
32	41123.0
33	54534.0
34	89685.0
35	121138.0
36	162204.0
37	299827.0
38	637603.0
39	369546.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.75235553373388	0.2001193897066769	0.0206151822161793	0.026909894343256947
8	99.10719999664282	0.8156897798004785	0.040653349154043145	0.036456874402658046
9	98.58153907809745	0.7136629874074284	0.26909894343256946	0.4356989910625579
10	58.556979209614965	30.349797152901704	3.873188827725269	7.220034809758062
11	31.20435678008689	24.180349797152903	22.01454917796305	22.60074424479716
12	25.483407663392367	17.673296047655167	30.360445707583345	26.48285058136912
13	22.091029930307045	17.795465918854866	31.967643081429447	28.145861069408646
14	23.23850349513891	19.107546205809808	31.56305045946153	26.090899839589753
15	23.419004365382857	18.892896522276462	30.72060815312097	26.96749095921971
16	28.349652374522783	19.361380472334215	26.19827713729082	26.090690015852186
17	30.120145072132154	18.652753254628447	22.941130803068884	28.285970870170512
18	27.92030055152169	19.69001690130206	24.104446060087223	28.285236487089023
19	27.80757274851261	19.872196361446566	23.288546456549174	29.031684433491645
20	27.116937916303407	19.74152862887531	24.004307681332296	29.13722577348898
21	28.939886548305093	19.29890545447297	25.836541013721426	25.92466698350051
22	30.10168058322606	19.094012574736592	23.79490359123818	27.009403250799167
23	28.56063014264867	20.504919842086654	24.6949425135415	26.239507501723175
24	27.236380078914706	20.14418038127071	24.10302974985863	28.516409789955947
25	27.193943227991323	21.220628610935382	22.886838910972838	28.698589250100454
26	28.858684761865796	19.784280215405047	22.728212165370483	28.628822857358678
27	28.147277379637238	18.92777971864735	23.568713602138523	29.356229299576892
28	29.069190426582146	19.06290620564195	23.506238584277277	28.361664783498625
29	29.06394483314292	20.883756600267944	22.940920979331313	27.111377587257824
30	28.398279025704458	20.244004024419286	22.491583445326754	28.866133504549502
31	29.17693491582396	21.295588141182	21.93854052902859	27.58893641396545
32	31.07174817794312	21.136331924366935	22.61527453862383	25.176645359066118
33	31.33701783816505	20.343932579436643	22.256948050789934	26.062101531608374
34	28.98924758256826	22.4564379692839	24.372233605159984	24.182080842987848
35	29.389171626375266	22.184349037590973	24.900674688228154	23.52580464780561
36	31.224237579221576	21.498120503870723	22.079909272215875	25.19773264469183
37	30.160536141614237	23.45414984142571	21.828802714279867	24.556511302680185
38	30.973235933154353	22.139027110276015	22.541783774540196	24.345953182029437
39	29.242872025355098	20.84530640035838	22.542937805096827	27.368883769189694
40	28.629767064177734	22.70843627810458	22.1698187437643	26.491977913953384
41	26.986584919338508	22.67103519688286	23.598718396610927	26.743661487167703
42	27.11452494332136	20.59635053573246	24.31631557909778	27.9728089418484
43	27.169918410039646	19.887565950223514	26.358739840596908	26.58377579913993
44	27.363061160472146	20.86665596565605	25.37015530103936	26.400127572832442
45	27.542985015437782	20.17303114518649	24.551737812650483	27.73224602672525
46	27.856514135300642	20.687256670034337	24.330740961055668	27.125488233609357
47	28.03329063420274	21.203265696651528	24.784222513877218	25.97922115526852
48	29.12064969822101	21.24753850527864	23.357945657750207	26.27386613875014
49	27.547024122385988	21.743719188695536	25.14700775613446	25.562248932784016
50	25.27604935473955	23.20886589220725	24.839143877185972	26.675940875867227
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	1.0
4	0.0
5	39.5
6	79.0
7	675.0
8	1271.0
9	1165.0
10	1059.0
11	1723.0
12	2387.0
13	2197.0
14	2007.0
15	2528.0
16	3049.0
17	3556.0
18	4063.0
19	3816.5
20	3570.0
21	5050.0
22	6530.0
23	7127.0
24	7724.0
25	9687.0
26	11650.0
27	11810.0
28	11970.0
29	17365.5
30	22761.0
31	23750.0
32	24739.0
33	27891.0
34	31043.0
35	32418.5
36	33794.0
37	37118.0
38	40442.0
39	42188.5
40	43935.0
41	50087.5
42	56240.0
43	62020.0
44	67800.0
45	72492.5
46	77185.0
47	84301.5
48	91418.0
49	99218.0
50	107018.0
51	120328.0
52	133638.0
53	156197.5
54	178757.0
55	181043.0
56	183329.0
57	180732.5
58	178136.0
59	166692.5
60	155249.0
61	140690.0
62	126131.0
63	107608.5
64	89086.0
65	77598.0
66	66110.0
67	59812.0
68	53514.0
69	45277.0
70	37040.0
71	34104.5
72	31169.0
73	22151.0
74	13133.0
75	9567.0
76	6001.0
77	4240.0
78	2479.0
79	1532.0
80	585.0
81	403.5
82	222.0
83	125.5
84	29.0
85	22.0
86	15.0
87	8.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1906362.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.276397183342335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.61259730736343	4.774233182390741
2	10.757505505313922	1.5655176551731178
3	4.758704314625566	1.0387866801390147
4	2.6528578647192846	0.7721298997860386
5	1.5242764178366788	0.5545620316690977
6	0.9756300171347179	0.42594429051979776
7	0.6312884247622471	0.3215453721071645
8	0.4678020306709632	0.27231307026688173
9	0.34590824890141225	0.2265269227201004
>10	5.020511237029264	10.090309256947684
>50	3.7903755776601766	20.01728553029084
>100	3.2309549409255665	41.94872224547334
>500	0.15865289563306828	7.784247645710572
>1k	0.06992757959182631	8.699906315759929
>5k	0.002255728373929881	0.9273123087157341
>10k+	7.519094579766269E-4	0.580657592329936
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	10613	0.5567148317056257	No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	5842	0.30644756871989687	No Hit
ACAATAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	5837	0.3061852890479353	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	5270	0.27644277424749336	No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	4578	0.2401432676480123	No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	4428	0.2322748774891652	No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	4240	0.22241316182341025	No Hit
ACAATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	4029	0.21134495966663203	No Hit
ACAATAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	3139	0.16465917805747282	No Hit
ACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	2895	0.15185993006574827	No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	2818	0.1478208231175401	No Hit
ACAATAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	2641	0.13853612273010057	No Hit
ACAATAGGGCAGTAGATTTCATTAAGAAAAATTGTGGACAAACTTGTTTC	2599	0.1363329734856234	No Hit
ACAATAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	2550	0.13376263270040004	No Hit
ACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG	2517	0.1320315868654537	No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	2450	0.12851703926116867	No Hit
ACAATAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	2431	0.1275203765077147	No Hit
ACAATAGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATG	2392	0.12547459506641448	No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2365	0.124058284837822	No Hit
ACAATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	2333	0.12237969493726795	No Hit
ACAATAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2305	0.12091092877428317	No Hit
ACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	2299	0.12059619316792927	No Hit
ACAATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	2293	0.1202814575615754	No Hit
ACAATAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT	2206	0.11571779126944409	No Hit
ACAATAGGGAGAAAATGAAGTTTTAGGACTTTCATGGTAACAAACCTGAC	2125	0.11146886058366669	No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	2057	0.10790185704498936	No Hit
ACAATAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA	2017	0.10580361966929681	No Hit
ACAATAGGGAGAGGACTTGTAGTTTATTCATAAATCTTCATGTTATTGTA	1972	0.10344310262164269	No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1936	0.1015546889835194	No Hit
ACAATAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1935	0.10150223304912709	No Hit
ACAATAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG	1923	0.10087276183641933	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.007815934224454747	0.0	0.0	0.0	0.0
7	0.00786839015884706	0.0	0.0	0.0	0.0
8	0.007973302027631688	0.0	0.0	0.0	0.0
9	0.00855031730594714	0.0	0.0	0.0	0.0
10	0.014058190417140081	0.0	0.0	0.0	0.0
11	0.02150693310084863	0.0	0.0	0.0	0.0
12	0.025650951917841416	0.0	0.0	0.0	0.0
13	0.031263736897818986	0.0	0.0	0.0	0.0
14	0.04060089321965083	0.0	0.0	0.0	0.0
15	0.048626651181674835	0.0	0.0	0.0	0.0
16	0.05712451255322966	0.0	0.0	0.0	0.0
17	0.0653076383184306	0.0	0.0	0.0	0.0
18	0.07055323175766198	0.0	0.0	0.0	0.0
19	0.07742495916305507	0.0	0.0	0.0	5.245593439231374E-5
20	0.08293283227424801	0.0	0.0	0.0	5.245593439231374E-5
21	0.08796860197591014	0.0	0.0	0.0	5.245593439231374E-5
22	0.09384366662784928	0.0	0.0	0.0	5.245593439231374E-5
23	0.10076784996763469	0.0	0.0	0.0	5.245593439231374E-5
24	0.10779694517620472	0.0	0.0	0.0	5.245593439231374E-5
25	0.11309499454982842	0.0	0.0	0.0	5.245593439231374E-5
26	0.11855041172662904	0.0	0.0	0.0	5.245593439231374E-5
27	0.12547459506641445	0.0	0.0	0.0	5.245593439231374E-5
28	0.13297579368451531	0.0	0.0	0.0	5.245593439231374E-5
29	0.13774928371421588	0.0	0.0	0.0	5.245593439231374E-5
30	0.14346698056297807	0.0	0.0	0.0	5.245593439231374E-5
31	0.1504960757715481	0.0	0.0	0.0	5.245593439231374E-5
32	0.16098726265001087	0.0	0.0	0.0	5.245593439231374E-5
33	0.16780653412101165	0.0	0.0	0.0	5.245593439231374E-5
34	0.1745733496576201	0.0	0.0	0.0	5.245593439231374E-5
35	0.18936592315625259	0.0	0.0	0.0	5.245593439231374E-5
36	0.20478796786759282	0.0	0.0	0.0	5.245593439231374E-5
37	0.21144987153541667	0.0	0.0	0.0	5.245593439231374E-5
38	0.21779703959688665	5.245593439231374E-5	0.0	0.0	5.245593439231374E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	7.857788E-4	44.0	19
ACCGATA	20	7.857788E-4	44.0	31
TATGCGC	40	8.3182385E-9	44.0	23
TTATAGG	45	4.802132E-10	44.0	29
AATTCGC	30	2.528599E-6	44.0	41
TCGTACG	20	7.857788E-4	44.0	36
CGTAAGG	25	4.4439475E-5	44.0	20
CGATATT	20	7.857788E-4	44.0	44
CGATACC	25	4.4439475E-5	44.0	36
GCGTAAG	20	7.857788E-4	44.0	37
GTACGTA	20	7.857788E-4	44.0	39
CCGTAAT	35	1.4467514E-7	43.999996	28
TAGGGAT	16975	0.0	42.9891	5
TAGGGGT	18655	0.0	42.950417	5
ATAGGGG	111945	0.0	42.846397	4
TAGGGGA	30190	0.0	42.826763	5
CAATAGG	194045	0.0	42.782345	2
AATAGGG	191400	0.0	42.778156	3
ACAATAG	195695	0.0	42.76788	1
TAGGGGC	28920	0.0	42.752422	5
>>END_MODULE