FastQCFastQC Report
Fri 17 Jun 2016
SRR1527194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029392
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG101060.4979816615025584No Hit
ACAATAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG53520.26372430757586507No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT53340.26283734241585655No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA50540.2490401065935019No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC43890.21627167151540955No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC42700.21040784629090878No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC39970.196955541364113No Hit
ACAATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG38120.18783951055291437No Hit
ACAATAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT30240.1490101468814305No Hit
ACAATAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT29490.14531445871472834No Hit
ACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT27670.13634625543019782No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC26390.13003894762569282No Hit
ACAATAGGGCAGTAGATTTCATTAAGAAAAATTGTGGACAAACTTGTTTCTG26320.12969401673013395No Hit
ACAATAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG24640.12141567523672114No Hit
ACAATAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC23830.11742433201668283No Hit
ACAATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA23310.11486198822110268No Hit
ACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA23280.11471416069443459No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG23090.11377791969220338No Hit
ACAATAGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC22870.11269385116330409No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA22540.11106774836995513No Hit
ACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA21770.1072735085188076No Hit
ACAATAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG21560.106238715832131No Hit
ACAATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT21450.10569668156768135No Hit
ACAATAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA20550.10126185576763878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA453.110472E-1046.036
CAATTCG206.3126034E-446.027
TACGCTT206.3126034E-446.023
CCGTAAT206.3126034E-446.031
CGTAAGC501.6370905E-1146.038
ATAACGT253.4180877E-546.031
TAAGCGT301.8621122E-646.033
GCGTAAT206.3126034E-446.09
GCGTAAG453.110472E-1046.037
GTACGTA206.3126034E-446.039
TAGGGGA320950.044.8677375
ATAGGGG1199700.044.7653584
CAATAGG2064950.044.707962
ACAATAG2084500.044.6836661
TAGGGGT200350.044.6683275
AGGGATC53200.044.659776
AATAGGG2037900.044.6479233
TAGGGAT177500.044.4321145
ATAGGGA618650.044.4199454
TAGGGGG370300.044.3478285
Produced by FastQC (version 0.11.5)