##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527194_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2029392
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42669479331741	33.0	31.0	34.0	31.0	34.0
2	32.880542053974786	34.0	31.0	34.0	31.0	34.0
3	32.95246113121566	34.0	31.0	34.0	31.0	34.0
4	36.438421458249564	37.0	37.0	37.0	35.0	37.0
5	36.295706792970506	37.0	37.0	37.0	35.0	37.0
6	36.33655400238101	37.0	37.0	37.0	35.0	37.0
7	36.44978200367401	37.0	36.0	37.0	35.0	37.0
8	36.601471770855504	37.0	37.0	37.0	35.0	37.0
9	38.72442780892011	39.0	39.0	39.0	39.0	39.0
10	38.210063900912196	39.0	39.0	39.0	37.0	39.0
11	37.91983805987212	39.0	38.0	39.0	35.0	39.0
12	37.6221799435496	39.0	37.0	39.0	35.0	39.0
13	37.57270157761536	39.0	37.0	39.0	35.0	39.0
14	38.42395850579878	40.0	38.0	40.0	35.0	40.0
15	38.33537187492609	40.0	38.0	40.0	35.0	40.0
16	33.25356954201061	40.0	35.0	40.0	3.0	40.0
17	35.01109297760117	40.0	35.0	40.0	17.0	40.0
18	37.1147466827503	40.0	35.0	40.0	32.0	40.0
19	37.95489831437199	40.0	37.0	40.0	34.0	40.0
20	38.185518125625805	40.0	38.0	40.0	34.0	40.0
21	38.360578439256685	40.0	38.0	40.0	35.0	40.0
22	38.37575096383547	40.0	38.0	40.0	35.0	40.0
23	38.34553600290136	40.0	38.0	40.0	35.0	40.0
24	38.296034477321285	40.0	38.0	40.0	35.0	40.0
25	38.26130141441377	40.0	38.0	40.0	35.0	40.0
26	38.16846030732357	40.0	38.0	40.0	35.0	40.0
27	38.05391959759376	40.0	38.0	40.0	34.0	40.0
28	37.91809468057428	40.0	38.0	40.0	34.0	40.0
29	37.72962394648249	40.0	38.0	40.0	33.0	40.0
30	37.758914492616505	40.0	37.0	40.0	34.0	40.0
31	37.692349728391555	40.0	37.0	40.0	33.0	40.0
32	37.61336597365122	40.0	37.0	40.0	33.0	40.0
33	37.28392050426926	40.0	37.0	40.0	33.0	40.0
34	37.230677956747634	40.0	36.0	40.0	33.0	40.0
35	37.174531091085406	39.0	36.0	40.0	32.0	40.0
36	37.1150305116015	39.0	36.0	40.0	32.0	40.0
37	36.75875779543824	39.0	35.0	40.0	31.0	40.0
38	36.73896861720161	39.0	35.0	40.0	31.0	40.0
39	36.36541781972137	39.0	35.0	40.0	31.0	40.0
40	36.32685060353052	39.0	35.0	40.0	30.0	40.0
41	36.294870089169564	38.0	35.0	40.0	31.0	40.0
42	36.21131747833834	38.0	35.0	40.0	30.0	40.0
43	36.08662939441961	38.0	35.0	40.0	30.0	40.0
44	36.38049277813256	39.0	35.0	40.0	31.0	40.0
45	36.423965897175115	39.0	35.0	40.0	31.0	40.0
46	36.33592080780845	38.0	35.0	40.0	31.0	40.0
47	36.022376159953325	38.0	35.0	40.0	31.0	40.0
48	35.942220132926515	38.0	35.0	40.0	31.0	40.0
49	35.82806229649077	37.0	35.0	40.0	30.0	40.0
50	35.33361322011716	37.0	34.0	40.0	29.0	40.0
51	35.390309018661746	37.0	34.0	40.0	29.0	40.0
52	35.033399658616965	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	6.0
16	24.0
17	73.0
18	191.0
19	345.0
20	609.0
21	1153.0
22	1907.0
23	2732.0
24	4348.0
25	6487.0
26	8924.0
27	11990.0
28	16140.0
29	21555.0
30	28203.0
31	37321.0
32	51206.0
33	73601.0
34	107276.0
35	152328.0
36	233317.0
37	408927.0
38	566929.0
39	293799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.78510805206683	0.18276409880397676	0.01177692629122417	0.020350922837973146
8	99.096330329478	0.8200485662700947	0.04464391305376191	0.038977191198151954
9	98.58593115573532	0.7198215031891325	0.2707214771714878	0.4235258639040658
10	58.999394892657506	29.972523790376627	3.7945355062008717	7.233545810764998
11	31.306864321924987	24.15792513225636	21.743261035817625	22.791949510001025
12	25.448163785015414	17.57955091968432	30.44675449592785	26.525530799372422
13	22.1794015153307	17.723682758185703	31.920545660966436	28.17637006551716
14	23.419773015760388	19.029147646191568	31.413497244494902	26.137582093553142
15	23.47540544162981	18.928674203899494	30.637846212067455	26.958074142403245
16	26.91121281644946	27.34360833195361	23.151170399804474	22.594008451792458
17	30.05944637605746	18.50652806357766	22.901440431419854	28.532585128945026
18	28.214854498293086	19.548120816480996	23.99368875012812	28.243335935097804
19	27.849178473158464	19.74512563368733	23.323241640846128	29.082454252308082
20	27.14024693110055	19.742464738207303	23.872618005786954	29.244670324905194
21	29.0649120524768	19.139870463665964	25.762149451658427	26.03306803219881
22	30.008199500145853	18.94069750940183	23.79175634869951	27.259346641752803
23	28.68445327467537	20.302435409226014	24.650190796061086	26.36292052003753
24	27.42092212840102	19.946318897482595	23.995807611343693	28.636951362772695
25	27.391997209016296	21.03107728817301	22.843492040965966	28.733433461844733
26	28.894811845124057	19.651600085148655	22.633182746359502	28.820405323367787
27	28.23707790313552	18.8568300259388	23.446480522245086	29.45961154868059
28	29.23304122614064	18.920297310721633	23.392572750853457	28.45408871228427
29	29.442167900533754	20.69945087001427	22.720844469673676	27.137536759778296
30	28.605020617012382	20.075520155790503	22.332304453747724	28.987154773449387
31	29.397031229057767	21.238282204719443	21.851273681969772	27.513412884253018
32	31.23038821479537	20.773512460874983	22.5615356717677	25.43456365256195
33	31.62075143688356	20.211964962905146	22.202265506122032	25.965018094089267
34	29.14932157020428	22.275144476769395	24.36611556564725	24.209418387379078
35	29.582653326710663	22.0740990405008	24.76658033539109	23.576667297397446
36	31.128633600605504	21.40335627616547	22.10790226826557	25.36010785496346
37	30.38653941673171	23.411494674266972	21.765188785606725	24.43677712339459
38	31.10153188738302	22.03497402177598	22.509648209907205	24.353845880933797
39	29.49765249887651	20.908331165196277	22.390647050939393	27.203369284987822
40	28.77255848056955	22.717493712402533	22.151462112790433	26.358485694237487
41	27.04085755733737	22.634611745783957	23.53502921071927	26.789501486159402
42	27.202728699038925	20.554924824775107	24.2800306692842	27.96231580690177
43	27.265358294503972	19.80233488650788	26.339908701719526	26.592398117268623
44	27.343509780269166	20.827223128897717	25.30073046508511	26.528536625748007
45	27.555740832722314	20.09680731963071	24.473290522481612	27.87416132516537
46	27.63635611059864	20.53856524515717	24.457472977128127	27.367605667116063
47	28.29211901889827	21.130417386093963	24.758991855688798	25.818471739318973
48	29.278079345932177	21.190041155183426	23.324572088586137	26.20730741029826
49	27.55820462483345	21.560841867909204	25.22597901243328	25.654974494824067
50	25.378487744112523	23.15732988008231	24.72154221559955	26.742640160205617
51	25.996505357269566	22.34501762104118	24.10175067212249	27.55672634956677
52	28.13517546141899	23.229716092307452	23.167628531106853	25.467479915166706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	39.5
6	78.0
7	678.5
8	1279.0
9	1198.5
10	1118.0
11	1714.5
12	2311.0
13	2236.0
14	2648.0
15	3135.0
16	3511.0
17	3887.0
18	3530.0
19	3173.0
20	4071.5
21	4970.0
22	6495.5
23	8021.0
24	8425.5
25	8830.0
26	10639.5
27	12449.0
28	14292.0
29	16135.0
30	19745.0
31	23355.0
32	24994.0
33	26633.0
34	29995.5
35	33358.0
36	34494.5
37	35631.0
38	37751.0
39	42148.0
40	44425.0
41	51706.5
42	58988.0
43	66969.5
44	74951.0
45	76402.0
46	77853.0
47	83763.5
48	89674.0
49	99808.0
50	109942.0
51	125128.0
52	140314.0
53	154160.0
54	168006.0
55	188930.0
56	209854.0
57	192350.5
58	174847.0
59	168935.5
60	163024.0
61	150892.0
62	138760.0
63	122482.0
64	89871.5
65	73539.0
66	66276.0
67	59013.0
68	53129.0
69	47245.0
70	41714.0
71	36183.0
72	27348.5
73	18514.0
74	12681.0
75	6848.0
76	4975.5
77	3103.0
78	2213.0
79	1323.0
80	793.0
81	263.0
82	184.5
83	106.0
84	56.5
85	7.0
86	6.0
87	5.0
88	3.5
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2029392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.778667893294589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.12067104514804	6.155660835603127
2	9.93492834927555	1.7443087304393496
3	4.146729991654817	1.0920829631970563
4	2.131258891169049	0.7483845600081743
5	1.2753905346944796	0.5598114969167124
6	0.7625016963893615	0.4016249496405568
7	0.5072513428411646	0.3117093754061209
8	0.3577845779038619	0.2512697589408472
9	0.29596498373141117	0.2338360470200155
>10	4.424676578496996	10.81272977161156
>50	3.3077152497120323	21.053143621655167
>100	2.558896028465749	40.0054440865039
>500	0.11813268748971543	6.938358673574455
>1k	0.05551590778205207	8.226961191448359
>5k	0.0019366014342576305	0.8919743010394157
>10k+	6.455338114192101E-4	0.5726996369951929
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	10106	0.4979816615025584	No Hit
ACAATAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	5352	0.26372430757586507	No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5334	0.26283734241585655	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5054	0.2490401065935019	No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4389	0.21627167151540955	No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4270	0.21040784629090878	No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3997	0.196955541364113	No Hit
ACAATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3812	0.18783951055291437	No Hit
ACAATAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	3024	0.1490101468814305	No Hit
ACAATAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	2949	0.14531445871472834	No Hit
ACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2767	0.13634625543019782	No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2639	0.13003894762569282	No Hit
ACAATAGGGCAGTAGATTTCATTAAGAAAAATTGTGGACAAACTTGTTTCTG	2632	0.12969401673013395	No Hit
ACAATAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2464	0.12141567523672114	No Hit
ACAATAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	2383	0.11742433201668283	No Hit
ACAATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2331	0.11486198822110268	No Hit
ACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA	2328	0.11471416069443459	No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2309	0.11377791969220338	No Hit
ACAATAGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC	2287	0.11269385116330409	No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	2254	0.11106774836995513	No Hit
ACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	2177	0.1072735085188076	No Hit
ACAATAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2156	0.106238715832131	No Hit
ACAATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2145	0.10569668156768135	No Hit
ACAATAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	2055	0.10126185576763878	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.008179789808967415	0.0	0.0	0.0	0.0
7	0.00822906565119011	0.0	0.0	0.0	0.0
8	0.008327617335635501	0.0	0.0	0.0	0.0
9	0.008820375757862453	0.0	0.0	0.0	0.0
10	0.0135508566112412	0.0	0.0	0.0	0.0
11	0.021632094735763222	0.0	0.0	0.0	0.0
12	0.024736472795793024	0.0	0.0	0.0	0.0
13	0.029023471069167514	0.0	0.0	0.0	0.0
14	0.038090226038143445	0.0	0.0	0.0	0.0
15	0.044939568107098087	0.0	0.0	0.0	0.0
16	0.05597735676498183	0.0	0.0	0.0	0.0
17	0.06208756120059604	0.0	0.0	0.0	0.0
18	0.06844414484732374	0.0	0.0	0.0	0.0
19	0.07460362512516064	0.0	0.0	0.0	0.0
20	0.07948193350520748	0.0	0.0	0.0	0.0
21	0.08352255256746849	0.0	0.0	0.0	0.0
22	0.08982986037197348	0.0	0.0	0.0	0.0
23	0.09677775412537351	0.0	0.0	0.0	0.0
24	0.10219809676986999	0.0	0.0	0.0	0.0
25	0.10687930178102605	0.0	0.0	0.0	0.0
26	0.11289095453219486	0.0	0.0	0.0	0.0
27	0.11855767638780482	0.0	0.0	0.0	0.0
28	0.12649108698565875	0.0	0.0	0.0	0.0
29	0.1323549122101595	0.0	0.0	0.0	0.0
30	0.13782453069687867	0.0	0.0	0.0	0.0
31	0.1450188036613922	0.0	0.0	0.0	0.0
32	0.15502179963259932	0.0	0.0	0.0	0.0
33	0.16083634901487737	0.0	0.0	0.0	0.0
34	0.16739003603049582	0.0	0.0	0.0	0.0
35	0.17990609995506043	0.0	0.0	0.0	0.0
36	0.19606857620410448	0.0	0.0	0.0	0.0
37	0.20178457390193713	0.0	0.0	0.0	0.0
38	0.20799333002199674	0.0	0.0	0.0	0.0
39	0.2143991895109471	0.0	0.0	0.0	0.0
40	0.22119925573767907	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	45	3.110472E-10	46.0	36
CAATTCG	20	6.3126034E-4	46.0	27
TACGCTT	20	6.3126034E-4	46.0	23
CCGTAAT	20	6.3126034E-4	46.0	31
CGTAAGC	50	1.6370905E-11	46.0	38
ATAACGT	25	3.4180877E-5	46.0	31
TAAGCGT	30	1.8621122E-6	46.0	33
GCGTAAT	20	6.3126034E-4	46.0	9
GCGTAAG	45	3.110472E-10	46.0	37
GTACGTA	20	6.3126034E-4	46.0	39
TAGGGGA	32095	0.0	44.867737	5
ATAGGGG	119970	0.0	44.765358	4
CAATAGG	206495	0.0	44.70796	2
ACAATAG	208450	0.0	44.683666	1
TAGGGGT	20035	0.0	44.668327	5
AGGGATC	5320	0.0	44.65977	6
AATAGGG	203790	0.0	44.647923	3
TAGGGAT	17750	0.0	44.432114	5
ATAGGGA	61865	0.0	44.419945	4
TAGGGGG	37030	0.0	44.347828	5
>>END_MODULE