FastQCFastQC Report
Fri 17 Jun 2016
SRR1527193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973419
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG99400.5036943497554245No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT56200.2847849341675539No Hit
ACAATAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG53620.27171117740327827No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA48990.24824935809374493No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC43040.21809863997458218No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC41230.20892674084925705No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC40200.20370737283871293No Hit
ACAATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG38220.19367402462426886No Hit
ACAATAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT29420.14908136589340631No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC27410.13889599725147067No Hit
ACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT26570.13463942528170653No Hit
ACAATAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT24760.12546752615638138No Hit
ACAATAGGGCAGTAGATTTCATTAAGAAAAATTGTGGACAAACTTGTTTCTG24760.12546752615638138No Hit
ACAATAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC23810.12065354595248146No Hit
ACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA23610.11964007643587093No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG22570.11437003494949628No Hit
ACAATAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG22060.11178568768213948No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA22030.11163366725464789No Hit
ACAATAGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC21960.11127895292383423No Hit
ACAATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT21260.10773180961569742No Hit
ACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA21050.10666766662325639No Hit
ACAATAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG20770.10524880930000166No Hit
ACAATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA20590.10433668673505221No Hit
ACAATAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA20320.10296850288762803No Hit
ACAATAGGGAGAAAATGAAGTTTTAGGACTTTCATGGTAACAAACCTGACAA20210.10241109465349224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG850.046.024
CTACGTA405.6152203E-946.015
CGGATAA206.3125615E-446.039
CGACTAA206.3125615E-446.014
TATCGGG206.3125615E-446.041
CGAATTA351.0200711E-745.99999646
TATACCA351.0200711E-745.99999631
ATAGGGG1160400.044.8008464
TAGGGGA306900.044.763445
TAGGGGT197650.044.7548685
TAGGGAT170600.044.7057465
CAATAGG2007750.044.700932
ACAATAG2027350.044.680591
TAGGGGC303250.044.6802985
AATAGGG1979300.044.6636663
ATAGGGA605000.044.3842964
ATAGGGC145200.044.3684584
TAGGGGG358000.044.2846375
TAGGGAG208150.044.232045
AGGGATC55550.043.7641756
Produced by FastQC (version 0.11.5)