##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527193_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1973419
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35038022842589	33.0	31.0	34.0	31.0	34.0
2	32.845288810941824	34.0	31.0	34.0	31.0	34.0
3	32.918619918020454	34.0	31.0	34.0	31.0	34.0
4	36.440532395806464	37.0	37.0	37.0	35.0	37.0
5	36.30781096158494	37.0	37.0	37.0	35.0	37.0
6	36.3255360366957	37.0	37.0	37.0	35.0	37.0
7	36.445610384819446	37.0	36.0	37.0	35.0	37.0
8	36.61992562147218	37.0	37.0	37.0	35.0	37.0
9	38.66939509551697	39.0	39.0	39.0	39.0	39.0
10	38.19982223744679	39.0	39.0	39.0	37.0	39.0
11	37.84519607848105	39.0	38.0	39.0	35.0	39.0
12	37.58435436164342	39.0	37.0	39.0	35.0	39.0
13	37.47735731742727	39.0	37.0	39.0	35.0	39.0
14	38.353807782331074	40.0	38.0	40.0	35.0	40.0
15	38.45109122796527	40.0	38.0	40.0	35.0	40.0
16	38.53832156272946	40.0	38.0	40.0	35.0	40.0
17	38.51930431398502	40.0	38.0	40.0	35.0	40.0
18	38.5047605196869	40.0	38.0	40.0	35.0	40.0
19	38.45421170060692	40.0	38.0	40.0	35.0	40.0
20	38.41102168368704	40.0	38.0	40.0	35.0	40.0
21	34.11289999741565	40.0	35.0	40.0	10.0	40.0
22	34.91357942737959	40.0	35.0	40.0	17.0	40.0
23	36.98012738298354	40.0	35.0	40.0	31.0	40.0
24	37.7133816994769	40.0	37.0	40.0	34.0	40.0
25	37.984638335801975	40.0	38.0	40.0	34.0	40.0
26	38.058307941699155	40.0	38.0	40.0	34.0	40.0
27	38.016479014340085	40.0	38.0	40.0	34.0	40.0
28	37.887116218096615	40.0	38.0	40.0	34.0	40.0
29	37.8637101396105	40.0	38.0	40.0	34.0	40.0
30	37.81898015576013	40.0	37.0	40.0	34.0	40.0
31	37.65374509924147	40.0	37.0	40.0	33.0	40.0
32	37.61084645480762	40.0	37.0	40.0	33.0	40.0
33	37.29585303475846	40.0	37.0	40.0	33.0	40.0
34	37.22369197823676	40.0	36.0	40.0	33.0	40.0
35	37.17351560920413	39.0	36.0	40.0	32.0	40.0
36	37.046980899646755	39.0	36.0	40.0	32.0	40.0
37	36.92083080177094	39.0	35.0	40.0	32.0	40.0
38	36.82405763803835	39.0	35.0	40.0	32.0	40.0
39	36.665458273179695	39.0	35.0	40.0	31.0	40.0
40	31.731224843786343	38.0	31.0	40.0	9.0	40.0
41	32.670467853000304	37.0	31.0	40.0	15.0	40.0
42	34.482741880969016	37.0	32.0	40.0	25.0	40.0
43	35.31766289875591	37.0	34.0	40.0	28.0	40.0
44	36.07304834908349	38.0	35.0	40.0	30.0	40.0
45	36.296693200987725	38.0	35.0	40.0	31.0	40.0
46	36.303848295775	38.0	35.0	40.0	31.0	40.0
47	36.2421817160978	38.0	35.0	40.0	31.0	40.0
48	36.06403455120276	38.0	35.0	40.0	31.0	40.0
49	35.88564922097132	37.0	35.0	40.0	31.0	40.0
50	35.784819645498494	37.0	35.0	40.0	31.0	40.0
51	35.58480890272162	37.0	35.0	40.0	30.0	40.0
52	35.17611769218802	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	4.0
15	9.0
16	36.0
17	93.0
18	183.0
19	351.0
20	680.0
21	1162.0
22	1928.0
23	3166.0
24	4610.0
25	6607.0
26	9216.0
27	12825.0
28	17326.0
29	22774.0
30	30200.0
31	41571.0
32	58402.0
33	76039.0
34	125688.0
35	184712.0
36	259146.0
37	275398.0
38	540749.0
39	300542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.77779680848315	0.190025534364471	0.012769715909292451	0.019407941243091305
8	99.12334886813191	0.8155895934923095	0.025488758342754377	0.03557278003302897
9	98.52084124050697	0.7490553197268294	0.3073853043879683	0.42271813537824454
10	59.01341782966516	29.92299151878035	3.804767259259184	7.258823392295301
11	31.292442203100308	24.231144019592392	21.718094332729137	22.758319444578166
12	25.444976459636802	17.593577440979335	30.440012992679204	26.521433106704656
13	22.251888727127895	17.660567776027293	31.957430226424293	28.130113270420527
14	23.305795677451165	18.94767406212264	31.55214376673175	26.194386493694445
15	23.41352748706686	18.806295064555474	30.721605497869437	27.05857195050823
16	28.13710621008514	19.209554585214796	26.300547425559394	26.352791779140674
17	30.02231153140818	18.56959925895109	22.87466574508505	28.53342346455568
18	28.067886242100638	19.61403026929405	24.055560425839623	28.262523062765688
19	27.87563107479962	19.695209177574554	23.3400509471126	29.08910880051322
20	27.115832978196725	19.661815357002236	23.88864199645387	29.33370966834717
21	33.67713597568484	18.605780120694085	24.396592918179056	23.320490985442017
22	30.111648869297397	18.892135932612387	23.782227697209766	27.213987500880453
23	28.70941244611509	20.286771334420113	24.603492719995096	26.400323499469703
24	27.48640810694536	20.036900425099788	23.95522694369518	28.52146452425967
25	27.303071471390517	21.04175545081911	22.87917568443397	28.775997393356402
26	28.912917125050484	19.62249273975775	22.642783919684568	28.821806215507202
27	28.13969055735249	18.906172485417443	23.52252613357832	29.431610823651745
28	29.22395091969825	18.971034534480513	23.392802035452178	28.412212510369063
29	29.35093865012955	20.761429782524644	22.683626741203973	27.204004826141837
30	28.477175906383795	20.1119478428048	22.355009250443015	29.055867000368394
31	29.361225365723143	21.2766270112936	21.82471132587656	27.537436297106698
32	31.299789857095732	20.740096249199993	22.523853271910323	25.436260621793956
33	31.62146508166791	20.251502595242066	22.13001901775548	25.997013305334548
34	29.128634111661032	22.2902485483316	24.364263240599183	24.216854099408184
35	29.575016760252133	22.151960632790097	24.749178963007857	23.523843643949917
36	31.198898966717152	21.388311352024076	22.128549486956395	25.284240194302377
37	30.23721774240544	23.42579046821785	21.716878169309204	24.620113620067507
38	31.07500231831152	22.02684782096453	22.520002087747205	24.378147772976746
39	29.327426157344185	20.832372648687382	22.351056719328234	27.489144474640202
40	28.965414846010905	27.2721606511339	20.35021452615993	23.41220997669527
41	27.080057504260374	22.648510022453415	23.5307859101387	26.740646563147514
42	27.17993492512234	20.582299045463735	24.264791207543862	27.97297482187006
43	27.229696278387916	19.919692675503782	26.237458948150394	26.613152097957908
44	27.32785080107164	20.843419466418432	25.34043707899843	26.488292653511497
45	27.515089294265433	20.09411077931245	24.4914536649338	27.89934626148831
46	27.54240229773809	20.506744893000423	24.50376731956062	27.447085489700868
47	28.12773161705649	21.10692154073717	24.830560565191682	25.934786277014666
48	29.25476039300321	21.208724553680693	23.22527552435646	26.31123952895964
49	27.58435993572576	21.61320023775995	25.114484050270114	25.687955776244177
50	25.369016919366842	23.10112550857167	24.857012119575213	26.67284545248627
51	26.018802899941672	22.383234376480615	24.065796467957387	27.53216625562032
52	28.08075730496159	23.31101504546171	23.071532198686644	25.536695450890058
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	39.0
6	76.0
7	622.5
8	1169.0
9	1123.0
10	1077.0
11	1710.0
12	2343.0
13	2191.0
14	2565.5
15	3092.0
16	3424.0
17	3756.0
18	3455.5
19	3155.0
20	3906.5
21	4658.0
22	6126.5
23	7595.0
24	7890.0
25	8185.0
26	9993.0
27	11801.0
28	13571.5
29	15342.0
30	18753.5
31	22165.0
32	24012.5
33	25860.0
34	28963.0
35	32066.0
36	33554.0
37	35042.0
38	36994.5
39	41086.0
40	43225.0
41	49536.0
42	55847.0
43	64051.0
44	72255.0
45	73808.0
46	75361.0
47	81881.0
48	88401.0
49	97504.5
50	106608.0
51	120754.0
52	134900.0
53	148262.5
54	161625.0
55	183469.5
56	205314.0
57	187483.5
58	169653.0
59	164961.5
60	160270.0
61	147387.5
62	134505.0
63	119566.0
64	88422.0
65	72217.0
66	64900.5
67	57584.0
68	51942.5
69	46301.0
70	40897.0
71	35493.0
72	27297.5
73	19102.0
74	13035.0
75	6968.0
76	5081.5
77	3195.0
78	2227.5
79	1260.0
80	745.5
81	231.0
82	159.0
83	87.0
84	48.0
85	9.0
86	7.5
87	6.0
88	4.5
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1973419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.947319851141483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01267935655262	6.3537315568962835
2	9.207801920030636	1.6477029780893757
3	4.009958356549756	1.0763514001742496
4	2.0807993604178763	0.7447030969483747
5	1.1627837200778743	0.5201898930618453
6	0.7672649977008122	0.4118979207008701
7	0.50521120075051	0.3164200343845844
8	0.3448911305655517	0.2468681007193432
9	0.2742113618330069	0.2208111085023302
>10	4.6687252214175725	11.62138419165885
>50	3.3097364010750683	21.267172424264672
>100	2.48509491142861	39.21791726827736
>500	0.11564693400619654	6.988778801185491
>1k	0.053223873036942725	8.136035808826637
>5k	0.001971254556923805	1.23003541630973
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	9940	0.5036943497554245	No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5620	0.2847849341675539	No Hit
ACAATAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	5362	0.27171117740327827	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4899	0.24824935809374493	No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4304	0.21809863997458218	No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4123	0.20892674084925705	No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4020	0.20370737283871293	No Hit
ACAATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3822	0.19367402462426886	No Hit
ACAATAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2942	0.14908136589340631	No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2741	0.13889599725147067	No Hit
ACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2657	0.13463942528170653	No Hit
ACAATAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	2476	0.12546752615638138	No Hit
ACAATAGGGCAGTAGATTTCATTAAGAAAAATTGTGGACAAACTTGTTTCTG	2476	0.12546752615638138	No Hit
ACAATAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	2381	0.12065354595248146	No Hit
ACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA	2361	0.11964007643587093	No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2257	0.11437003494949628	No Hit
ACAATAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2206	0.11178568768213948	No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	2203	0.11163366725464789	No Hit
ACAATAGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC	2196	0.11127895292383423	No Hit
ACAATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2126	0.10773180961569742	No Hit
ACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	2105	0.10666766662325639	No Hit
ACAATAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2077	0.10524880930000166	No Hit
ACAATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2059	0.10433668673505221	No Hit
ACAATAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	2032	0.10296850288762803	No Hit
ACAATAGGGAGAAAATGAAGTTTTAGGACTTTCATGGTAACAAACCTGACAA	2021	0.10241109465349224	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.009526613456138812	0.0	0.0	0.0	0.0
7	0.009627960407799865	0.0	0.0	0.0	0.0
8	0.00977998083529144	0.0	0.0	0.0	0.0
9	0.01003334821444407	0.0	0.0	0.0	0.0
10	0.01601281836244609	0.0	0.0	0.0	0.0
11	0.0233604723578723	0.0	0.0	0.0	0.0
12	0.027110309569331196	0.0	0.0	0.0	0.0
13	0.03096149373245114	0.0	0.0	0.0	0.0
14	0.04003204590611523	0.0	0.0	0.0	0.0
15	0.04667027123991408	0.0	0.0	0.0	0.0
16	0.056703619454358145	0.0	0.0	0.0	0.0
17	0.06253106917486859	0.0	0.0	0.0	0.0
18	0.06795313108873482	0.0	0.0	0.0	0.0
19	0.0736285603817537	0.0	0.0	0.0	5.0673475830525603E-5
20	0.07768243844819575	0.0	0.0	0.0	5.0673475830525603E-5
21	0.0829524799345704	0.0	0.0	0.0	5.0673475830525603E-5
22	0.08964137874419979	0.0	0.0	0.0	5.0673475830525603E-5
23	0.09627960407799864	0.0	0.0	0.0	5.0673475830525603E-5
24	0.10246176812932277	0.0	0.0	0.0	5.0673475830525603E-5
25	0.10864393218064688	0.0	0.0	0.0	5.0673475830525603E-5
26	0.11457272885281838	0.0	0.0	0.0	5.0673475830525603E-5
27	0.12318721974400773	0.0	0.0	0.0	5.0673475830525603E-5
28	0.13251113929682445	0.0	0.0	0.0	5.0673475830525603E-5
29	0.13707175212157174	0.0	0.0	0.0	5.0673475830525603E-5
30	0.1422911201321159	0.0	0.0	0.0	5.0673475830525603E-5
31	0.14887867199008423	0.0	0.0	0.0	5.0673475830525603E-5
32	0.15820259154290092	0.0	0.0	0.0	5.0673475830525603E-5
33	0.16397936778758085	0.0	0.0	0.0	5.0673475830525603E-5
34	0.16995883793558286	0.0	0.0	0.0	5.0673475830525603E-5
35	0.18303259469985847	0.0	0.0	0.0	5.0673475830525603E-5
36	0.19651173927077828	0.0	0.0	0.0	5.0673475830525603E-5
37	0.20244053594294978	0.0	0.0	0.0	5.0673475830525603E-5
38	0.2080146182843076	0.0	0.0	0.0	5.0673475830525603E-5
39	0.21541294575556433	0.0	0.0	0.0	5.0673475830525603E-5
40	0.22331800798512633	0.0	0.0	0.0	5.0673475830525603E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	85	0.0	46.0	24
CTACGTA	40	5.6152203E-9	46.0	15
CGGATAA	20	6.3125615E-4	46.0	39
CGACTAA	20	6.3125615E-4	46.0	14
TATCGGG	20	6.3125615E-4	46.0	41
CGAATTA	35	1.0200711E-7	45.999996	46
TATACCA	35	1.0200711E-7	45.999996	31
ATAGGGG	116040	0.0	44.800846	4
TAGGGGA	30690	0.0	44.76344	5
TAGGGGT	19765	0.0	44.754868	5
TAGGGAT	17060	0.0	44.705746	5
CAATAGG	200775	0.0	44.70093	2
ACAATAG	202735	0.0	44.68059	1
TAGGGGC	30325	0.0	44.680298	5
AATAGGG	197930	0.0	44.663666	3
ATAGGGA	60500	0.0	44.384296	4
ATAGGGC	14520	0.0	44.368458	4
TAGGGGG	35800	0.0	44.284637	5
TAGGGAG	20815	0.0	44.23204	5
AGGGATC	5555	0.0	43.764175	6
>>END_MODULE