FastQCFastQC Report
Fri 17 Jun 2016
SRR1527192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15930
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGAACAACTGGGACTAGCATTGAATAAAAAGTAATGTCTAAAT7134.475831763967357No Hit
AGCGAGGGGAGCTCGTCACACTAGCACCAAGAGGACACTGTCAGCCTTCC6123.84180790960452No Hit
AGCGAGGGGATGTGGAACAATGTATTCAGTATACTTAGTGTACCACGTTT5923.716258631512869No Hit
AGCGAGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG4432.780916509730069No Hit
AGCGAGGGGCGTCTGATCTATGACACCAAGGGTCGCTTTGCTGTTCATCG3352.102950408035154No Hit
AGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC3322.084118016321406No Hit
AGCGAGGGGAACCCACGCTCCCCTCCCCCACGCCCTCGTCTTCCAACAAG3121.9585687382297552No Hit
AGCGAGGGGGACGGTGTATTAAAGCATCTCATCTGGCCAGGTCCTGGAAC2461.544256120527307No Hit
AGCGAGGGAGCCCACTCTGGTCTCCTCCGTGGCCCCATCGCAGACCAGAG2451.5379786566227243No Hit
AGCGAGGGGGCTTCTTGAATAAACATACAGACTGATGGATAAACGTGTGG2171.362209667294413No Hit
AGCGAGGGGCTGAGGCAAGAGAGTCACTTGAACCCAGGAGGCAGAGGTTG2041.28060263653484No Hit
AGCGAGGGTATCCTACAAAGTCCAGCGTACCATAAACGCAAGCCTCAACG1701.0671688637790333No Hit
AGCGAGGGGATTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCT1510.9478970495919649No Hit
AGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG1460.9165097300690521No Hit
AGCGAGGGGATTTTTAGGGGTTTCTTTTGCGGGACAGAGGAACAGATCTT1460.9165097300690521No Hit
AGCGAGGGGTGACCTCTGAGTTATTCTATCCCCGGGGGTAAAAAGACTGT1440.903954802259887No Hit
AGCGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA1380.8662900188323917No Hit
AGCGAGGGGCAAAGGACTCAAGGAATTCCTAGGCTCAGGGGGCCACTGCT1320.8286252354048964No Hit
AGCGAGGGGGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGC1310.8223477715003138No Hit
AGCGAGGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGG1310.8223477715003138No Hit
AGCGAGGGGAAGGTATGTCCTGACCTCAGTGGAGCCCCTCTGAGTGTGTG1290.8097928436911487No Hit
AGCGAGGGGGTTTTTTATTTTTTGTTTTTTTAATTGTATGTTTGTGTGTT1260.7909604519774011No Hit
AGCGAGGGGATGAGACTCTGTTTTGAAAAGACAAAGGGAAAAAGGAAGGT1260.7909604519774011No Hit
AGCGAGGGGGGCTTTGGACAGGGGCCAGGAAAGGGCTGAGCCGGAGGAAA1200.7532956685499058No Hit
AGCGAGAGGCCACGGTGTATTAAAGCATCTCATCTGGCCAGGTCCTGGAA1190.7470182046453233No Hit
AGCGAGGGGGGGGGATTAGCTCAGCTGGGAGAGCACCTGCTTTGCAAGCA1180.7407407407407408No Hit
AGCGAGGGGGAATGTTTTAATGTTGAATTTTGGACATGTTATTACTGTTC1160.7281858129315757No Hit
AGCGAGGGGTATCACTGAAGGAGCTGATCTGCTGGAGGGACTTCAGGATG1160.7281858129315757No Hit
AGCGAGGGGGACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCC1140.7156308851224105No Hit
AGCGAGGGGGATTTTGGTTCAGCCACACCCAGATCCCACCCTCTCTTTCT1140.7156308851224105No Hit
AGCGAGGGGCCCTTTCTGCGCAGGAAGGCTGAGGTGCTAGCACCAGCCTG1140.7156308851224105No Hit
AGCGAGGGGGTTCATGGATTGGTTAACATAGTGAAAATGGCCATCTTATC1120.7030759573132455No Hit
AGCGAGGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA1110.696798493408663No Hit
AGCGAGGGAGGGAGGAAGGGAGGAAAGAATTGGTCAAGTCAGGTAAGAAG1110.696798493408663No Hit
AGCGAGGGGACTCAAAGAAAGCATGAAAGCCTACAGAGAGTCACTTTCGG1100.6905210295040803No Hit
AGCGAGGGGAGTTAGAACAGCCTCTGTGGTGGGCCGGGAAGGTTTCTGAG1090.6842435655994978No Hit
AGCGAGGGGAGCATGGCAGGTGGAAGCTGTCCCCAGTCCCAGGCCTCAAC1090.6842435655994978No Hit
AGCGAGGGGGTCACCTCTACAATCCTGTAATGAATTGCAGCTAGCAAAGA1080.6779661016949152No Hit
AGCGAGGGGATTGATGAACCCATACAAAGGGAAAACTCAGACGCCAGTAA1050.6591337099811676No Hit
AGCGAGGGGGCCACGGTGTATTAAAGCATCTCATCTGGCCAGGTCCTGGA1030.6465787821720025No Hit
AGCGAGGGGCGCAAGAGGAATGGGGGAAAGCAGCAATGGTGAAGCATTTC1030.6465787821720025No Hit
AGCGAGGGGGGTCGAGACTCCTCTACCCTGTGCAAAGGTGTATGAGTTTC1020.64030131826742No Hit
AGCGAGGGGGTTCGCGTTTCGCCATGTCGGGGTCCCCGGTAAAGCGCCAG1000.6277463904582549No Hit
AGCGAGGGGTCCTTGGCAGACATAGGTGTTTTCCCAGCTCTAGGGGTCTG940.5900816070307595No Hit
AGCGAGGGGACCTTTCTCATCTCTGTCGGTCAATGGTGGAGCCAGCTACG930.583804143126177No Hit
AGCGAGGGGGAGTTCGGCTCAGCACCTATATCCCATCCAAGACAAAGGCA920.5775266792215945No Hit
AGCGAGGGGATGCCAATTTTGTCAACAGGAAACTTCACAAAACACACCCT900.5649717514124294No Hit
AGCGAGGGGCCTCGGGAGGCTGAGGCAGGGGAATTTCTTGAACACAGGAG900.5649717514124294No Hit
AGCGAGGGGGGCTGGTGGGCAGGCTAAGAGTGTGGACAGCCTGCACTCCA880.5524168236032643No Hit
AGCGAGGGGGTCACACCAGGTCCCACCAGCCCCTGGACTGTGGTGTCCAG880.5524168236032643No Hit
AGCGAGGGGGAGGAAGAAGGAGAGGAATACTAAATTAAATGTCACAAGGT870.5461393596986817No Hit
AGCGAGGGGGACGTAGTAATGTAATATTTGTAAAGCTTTGCTCTCACTGC870.5461393596986817No Hit
AGCGAGGGGAGAGTGACAGACTTGTGAGGCACCATGAGCGTTCTGGGCAT860.5398618957940993No Hit
AGCGAGGAGGGAAGGCCGAGGCAGGCAGATCACTTGAGGTCAGAAGTTTG850.5335844318895167No Hit
AGCGAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCCAA850.5335844318895167No Hit
AGCGAGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGC850.5335844318895167No Hit
AGCGAGGGGGAGTGCAGTGCTAGCGAGGGGTATTGTATTAAGATTTTATA840.527306967984934No Hit
AGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA840.527306967984934No Hit
AGCGAGGGGATAGTAGGTGCACATCTAGAGGGTGGGAGGCCATGAGAGAG790.49591964846202136No Hit
AGCGAGGGGACTTCTAGCATATTCTTATTTGATGCTTTCTGGAAGTACAT760.4770872567482737No Hit
AGCGAGGGGAGGCCCAGAGATGGCAACCACTCTCAGCCTCCAGTTCTGCT750.4708097928436911No Hit
AGCGAGGGGGATCTCAGGGATTACACACCCTAAATCAGTTCAAGAGAGGC750.4708097928436911No Hit
AGCGAGGGGCAATTCTGCTACCAAATATATACTCTAGTGAAAGGTTCATG730.45825486503452606No Hit
AGCGAGGGGATAGCAAGATTCTTTCCTATCCAGTTGAAATTAAAGATTTC710.44569993722536094No Hit
AGCGAGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTGTAAGTAG690.4331450094161958No Hit
AGCGAGGGGCCCGAAGCCACTCACTCATCCTTACCTCTCTCCAGGTGAGC690.4331450094161958No Hit
AGCGAGGGGATTGTTTTCTGAAAAGTTAAAGAAGTGGGTATGCTTGTCTT690.4331450094161958No Hit
AGCGAGGGGTACAAGATAAGAGCTTAATGGCTCAACTTCAAGAAACAATG680.42686754551161327No Hit
AGCGAGGGGACATTCATTCTAGTTATAATAAATTTAATGTTTGTAAATGT670.4205900816070308No Hit
AGCGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGC620.389202762084118No Hit
AGCGAGGGGGTCTCCACAACTTCCCTTCATTGCGAGGTTTTATGGAAAGT610.38292529817953547No Hit
AGCGAGGGGACTCTCCCGTTGGGTCACACTAAAATTGATTTTTCCAAGCA590.3703703703703704No Hit
AGCGAGGGGGAGAACAACCCACGTGGGCATGGCCACAGTCATGGCAAAGG590.3703703703703704No Hit
AGCGAGGGAACTTCCTTATTGGAGAGGACGGTCATGTCTATGAAGGCCGA590.3703703703703704No Hit
AGCGAGGGGAAGCTTTCTAGATGCCTAATATCCTTTGCTTAATACTTGAT570.35781544256120523No Hit
AGCGAGGGGACTGTGTGGCCCAGGCTGATCTCATGTCTCCTGCTCTCCTG560.35153797865662273No Hit
AGCGAGGGGGATGCCGAGTGGCGCGTTTTTATGTGGACAGAATTCTACTC550.34526051475204017No Hit
AGCGAGGGGATCTCTAAGTCCCGAGAGTTATCACTTATTTTTTTTTTAAC540.3389830508474576No Hit
AGCGAGGGGAAAGCCATAGATTTAAAAACAAACAAACAAACAAACAAACA530.3327055869428751No Hit
AGCGAGGGGATTTGTGGTATGACTGTGTGTGTGTGTGTGTGTGTGTGTGT530.3327055869428751No Hit
AGCGAGGGGAGAAGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGACT520.32642812303829255No Hit
AGCGAGGGGTGCGGGACTCTGCTGCCAGTGAGTGCTGCTGTCTCCCATGG520.32642812303829255No Hit
AGCGAGGGGCGTGCGCCTTAGTTTAGTACTCTGAATTTACTTTTAAGTCG510.32015065913371No Hit
AGCGAGGGGCTAGCGAGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACAT500.31387319522912743No Hit
AGCGAGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGA460.28876333961079725No Hit
AGCGAGGGGAGTCTAATTATAGCACAATGTGAGCTGTTTTTTAAAAAATC460.28876333961079725No Hit
AGCGAGGGGACACACGTCTGAGGGACACCTTGGAGCCACGCCCTTTTTCG460.28876333961079725No Hit
AGCGAGGGGAGTGAAAAAATGGGTTATAGTGAGTACTTTGAAACAACAAA450.2824858757062147No Hit
AGCGAGGGGGCTAGCGAGGGGAGAGTATTGCTCATCCCTCCAGAAGCAAG450.2824858757062147No Hit
AGCGAGGGGATCATCTTCATCGATGAGATTGATTCTCTGTGTGGTTCCAG440.27620841180163214No Hit
AGCGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.27620841180163214No Hit
AGCGAGGGGATCAACATCGGCATCGGGGGCTCTGACCTGGGACCCCTCAT440.27620841180163214No Hit
AGCGAGGGGCCTTCCCCAGTCAGTCACTAATTAAGAAAATGCCCAACAGA430.26993094789704963No Hit
AGCGAGGGGACTTGGTACCTTGTACACTTATTACTGTCTCAGTCGTTGCT410.25737602008788446No Hit
AGCGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTT410.25737602008788446No Hit
AGCGAGGGGTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.25737602008788446No Hit
AGCGAGGGGATCCAGAAGGAGTCAACCCTGCACCTGGTCCTTCGCCTGAG400.25109855618330196No Hit
AGCGAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT400.25109855618330196No Hit
AGCGAGGGGCCCGGGCCAGCCTCCACCTCTGATGTTTGGACACGGCTGCC380.23854362837413684No Hit
AGCGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT380.23854362837413684No Hit
AGCGAGGGGACCTAAATAATTTAGTAAGAAAAATGTCCAACAGTTTTAAC380.23854362837413684No Hit
AGCGAGGGGACTTGAAAGTTCTCAGTAACTTTTTTGGCAGCTCAATAGGA380.23854362837413684No Hit
AGCGAGGGGAGCTAATGCTCTCCAGACGTGGTTGCCAAATCACTACCTAT370.2322661644695543No Hit
AGCGAGGGGGAGGAAGAAGGAGAGGAGTACTAAGTCCATTCCTTGAGCCC370.2322661644695543No Hit
AGCGAGGGGATGAATATAATACTCAATTTCAAGAAAAAAGTCGGGAATAT360.22598870056497175No Hit
AGCGAGGGGCCTAGAAGACAAGCCATGTGCTACAAAAGGAAAGGAATGGA340.21343377275580663No Hit
AGCGAGGGGATTCCCACTGTACTCGCTTCCAGCCGCCTTTACAAGTCTCT340.21343377275580663No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.21343377275580663No Hit
AGCGAGGGGAGGCATTGTGTGTGTGTGTGGCATTTGTCATGATCCAGACT330.2071563088512241No Hit
AGCGAGGGGTACCTATGCTTGCTAGAATCTTGGGTTTTCTGTAGCCCCAC330.2071563088512241No Hit
AGCGAGGGGGGCTTCTGCCAACCTAGAGCTGAACGGTGCACCATCTCAGT310.194601381042059No Hit
AGCGAGGGGATTCTACACAAAGCAGTTGGGGGGGTATTTTTGTTTAGAAA300.18832391713747645No Hit
AGCGAGGGGGTGCAGTGCTAGCGAGGGGAAGCAGTAGTATCAATGCAAAA300.18832391713747645No Hit
AGCGAGGGGACAGAAAATGTGGTACATTTACACAACGGAGTACTACTCAG300.18832391713747645No Hit
AGCGAGGGGACATCAGCTTCACATCGTGGCTTTGTGTGGTTAAAAGTGCT300.18832391713747645No Hit
AGCGAGGGGATAAATGAAATAAATAAATCTTCCTTTTTTAAAGTTGTTGG300.18832391713747645No Hit
AGCGAGGGGATGCCTTTGTCTCACAGGGCACTCAGTTCCTGCTCTGCACC290.18204645323289392No Hit
AGCGAGGGGAAGCCCCAGTGACTGTATTGAAGATTCTCAGGGTCACGGAG290.18204645323289392No Hit
AGCGAGGGGAATATCTTTACGTGTATGTTCTGTGAGTGGCTTGGTTATTC280.17576898932831136No Hit
AGCGAGGGAAGAAAGGACCCCCTCCTTATGGATTGCCTTGGCTTTGACCC260.16321406151914628No Hit
AGCGAGGGGGGAGGAAGACTCGTTAGGGTGAGAACTGCATTTCAAAAGGT250.15693659761456372No Hit
AGCGAGGGGAATATCTTTACGTGTATGTTCTGTGAGTGGCTTGGTTATTT250.15693659761456372No Hit
AGCGAGGGGACCTGAGAACTTTTTTCGTTGCTTGGTTTTGTTTTTTTTTC250.15693659761456372No Hit
AGCGAGGGGGGCAGTTGCGACAGGGGCAGGGTGTCCTACCAAGGAACACT240.15065913370998116No Hit
AGCGAGGGGATCCATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGAC240.15065913370998116No Hit
AGCGAGGGGGATTTACAGTCGGCAGCCCAATTCCCAACAATGGTTCAGTC240.15065913370998116No Hit
AGCGAGGGGGTGGCTGCATGACTAAAGGCACATCCTTTTCTTAATGCAGA230.14438166980539863No Hit
AGCGAGGGGCATGGTTGATCAGCGCCAGCAGACTGTTGATCGCCGTGGCC220.13810420590081607No Hit
AGCGAGGGGATGTGGAACAATGTATTCAGTATACTTAGTGTACCTCGTTT210.1318267419962335No Hit
AGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.12554927809165098No Hit
AGCGAGGGGATGTGGAACAATGTATTCAGTATACTTAGTGCACCACGTTT200.12554927809165098No Hit
AGCGAGGGGGGGGATTAGCTCAGCTGGGAGAGCACCTGCTTTGCAAGCAG190.11927181418706842No Hit
AGCGAGGGGGACTTACCCTGAAGTGGCAGCCGCTGGAGGGGGCGCCTGGT190.11927181418706842No Hit
AGCGAGAGGGATGTCACACGCAGATGAGATCATAGAGTATTTTTTTTTCT190.11927181418706842No Hit
AGCGAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT180.11299435028248588No Hit
AGCGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.10671688637790332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGA551.8189894E-1244.00000440
TTCATCG551.8189894E-1244.00000444
GGGTCGC551.8189894E-1244.00000430
GTTTGAA551.8189894E-1244.00000441
TCAAGAC551.8189894E-1244.00000431
GTCGCTT551.8189894E-1244.00000432
GGCGTCT551.8189894E-1244.0000048
CGCTTTG551.8189894E-1244.00000434
TGCTGTT551.8189894E-1244.00000439
TGTTCAT551.8189894E-1244.00000442
AGGGTCG551.8189894E-1244.00000429
TGACACC551.8189894E-1244.00000421
GTTCATC551.8189894E-1244.00000443
TATGACA551.8189894E-1244.00000419
GCTGTTC551.8189894E-1244.00000440
TCGCTTT551.8189894E-1244.00000433
TCGTTTG551.8189894E-1244.00000439
GCGTCTG551.8189894E-1244.0000049
ATCGTTT551.8189894E-1244.00000438
ACTAGCA1750.044.00000420
Produced by FastQC (version 0.11.5)