FastQCFastQC Report
Fri 17 Jun 2016
SRR1527191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17104
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGAACAACTGGGACTAGCATTGAATAAAAAGTAATGTCTAAATCA6163.601496725912067No Hit
AGCGAGGGGATGTGGAACAATGTATTCAGTATACTTAGTGTACCACGTTTAA5963.4845650140318054No Hit
AGCGAGGGGAGCTCGTCACACTAGCACCAAGAGGACACTGTCAGCCTTCCTG5333.11623012160898No Hit
AGCGAGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA4002.3386342376052385No Hit
AGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG3622.1164639850327407No Hit
AGCGAGGGGCGTCTGATCTATGACACCAAGGGTCGCTTTGCTGTTCATCGTA3502.0463049579045838No Hit
AGCGAGGGGAACCCACGCTCCCCTCCCCCACGCCCTCGTCTTCCAACAAGCA2761.6136576239476148No Hit
AGCGAGGGGGACGGTGTATTAAAGCATCTCATCTGGCCAGGTCCTGGAACCT2391.39733395696913No Hit
AGCGAGGGAGCCCACTCTGGTCTCCTCCGTGGCCCCATCGCAGACCAGAGCG2041.1927034611786715No Hit
AGCGAGGGGCTGAGGCAAGAGAGTCACTTGAACCCAGGAGGCAGAGGTTGCA1821.0640785781103836No Hit
AGCGAGGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC1490.8711412535079514No Hit
AGCGAGGGGAAGGTATGTCCTGACCTCAGTGGAGCCCCTCTGAGTGTGTGAA1410.8243685687558465No Hit
AGCGAGGGGGCTTCTTGAATAAACATACAGACTGATGGATAAACGTGTGGAT1410.8243685687558465No Hit
AGCGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA1380.8068288119738074No Hit
AGCGAGGGGTGACCTCTGAGTTATTCTATCCCCGGGGGTAAAAAGACTGTCT1370.8009822263797941No Hit
AGCGAGGGGATTTTTAGGGGTTTCTTTTGCGGGACAGAGGAACAGATCTTGA1360.7951356407857811No Hit
AGCGAGGGGATTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCA1360.7951356407857811No Hit
AGCGAGGGGGTTTTTTATTTTTTGTTTTTTTAATTGTATGTTTGTGTGTTTA1320.7717492984097287No Hit
AGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1300.7600561272217026No Hit
AGCGAGAGGCCACGGTGTATTAAAGCATCTCATCTGGCCAGGTCCTGGAACC1240.7249766136576239No Hit
AGCGAGGGGCAAAGGACTCAAGGAATTCCTAGGCTCAGGGGGCCACTGCTGT1240.7249766136576239No Hit
AGCGAGGGGGACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTC1230.7191300280636108No Hit
AGCGAGGGGATGAGACTCTGTTTTGAAAAGACAAAGGGAAAAAGGAAGGTAG1210.7074368568755847No Hit
AGCGAGGGGGGGGGATTAGCTCAGCTGGGAGAGCACCTGCTTTGCAAGCAGG1170.6840505144995322No Hit
AGCGAGGGGAGCATGGCAGGTGGAAGCTGTCCCCAGTCCCAGGCCTCAACAG1160.6782039289055192No Hit
AGCGAGGGGTCCTTGGCAGACATAGGTGTTTTCCCAGCTCTAGGGGTCTGAC1160.6782039289055192No Hit
AGCGAGGGGAGTTAGAACAGCCTCTGTGGTGGGCCGGGAAGGTTTCTGAGGG1140.666510757717493No Hit
AGCGAGGGAGGGAGGAAGGGAGGAAAGAATTGGTCAAGTCAGGTAAGAAGAA1140.666510757717493No Hit
AGCGAGGGGGAATGTTTTAATGTTGAATTTTGGACATGTTATTACTGTTCAA1140.666510757717493No Hit
AGCGAGGGGACTCAAAGAAAGCATGAAAGCCTACAGAGAGTCACTTTCGGAT1130.6606641721234799No Hit
AGCGAGGGGGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAG1070.6255846585594014No Hit
AGCGAGGGGGGCTTTGGACAGGGGCCAGGAAAGGGCTGAGCCGGAGGAAATG1050.6138914873713751No Hit
AGCGAGGGGCGCAAGAGGAATGGGGGAAAGCAGCAATGGTGAAGCATTTCTT1050.6138914873713751No Hit
AGCGAGGGGGTTCGCGTTTCGCCATGTCGGGGTCCCCGGTAAAGCGCCAGAG1040.608044901777362No Hit
AGCGAGGGGCCCTTTCTGCGCAGGAAGGCTGAGGTGCTAGCACCAGCCTGGT1030.602198316183349No Hit
AGCGAGGGGTATCACTGAAGGAGCTGATCTGCTGGAGGGACTTCAGGATGTG980.5729653882132835No Hit
AGCGAGGGGGCCACGGTGTATTAAAGCATCTCATCTGGCCAGGTCCTGGAAC970.5671188026192703No Hit
AGCGAGGGGGGCTGGTGGGCAGGCTAAGAGTGTGGACAGCCTGCACTCCAAG960.5612722170252572No Hit
AGCGAGGGGCCTCGGGAGGCTGAGGCAGGGGAATTTCTTGAACACAGGAGGC960.5612722170252572No Hit
AGCGAGGGGGGTCGAGACTCCTCTACCCTGTGCAAAGGTGTATGAGTTTCGA960.5612722170252572No Hit
AGCGAGGGGATTGATGAACCCATACAAAGGGAAAACTCAGACGCCAGTAAGC960.5612722170252572No Hit
AGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG940.549579045837231No Hit
AGCGAGGGGGTCACCTCTACAATCCTGTAATGAATTGCAGCTAGCAAAGATA940.549579045837231No Hit
AGCGAGGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTATC940.549579045837231No Hit
AGCGAGGGGAGAGTGACAGACTTGTGAGGCACCATGAGCGTTCTGGGCATTG920.5378858746492049No Hit
AGCGAGGGGATGCCAATTTTGTCAACAGGAAACTTCACAAAACACACCCTCC890.5203461178671656No Hit
AGCGAGGGGGTTCATGGATTGGTTAACATAGTGAAAATGGCCATCTTATCAA860.5028063610851263No Hit
AGCGAGGAGGGAAGGCCGAGGCAGGCAGATCACTTGAGGTCAGAAGTTTGAG860.5028063610851263No Hit
AGCGAGGGGACATTCATTCTAGTTATAATAAATTTAATGTTTGTAAATGTAA840.49111318989710007No Hit
AGCGAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCCAAGG820.4794200187090739No Hit
AGCGAGGGGAGGCCCAGAGATGGCAACCACTCTCAGCCTCCAGTTCTGCTGC810.4735734331150608No Hit
AGCGAGGGGGAGTGCAGTGCTAGCGAGGGGTATTGTATTAAGATTTTATAGT800.4677268475210477No Hit
AGCGAGGGGACCTTTCTCATCTCTGTCGGTCAATGGTGGAGCCAGCTACGGG790.4618802619270346No Hit
AGCGAGGGGAACAACAGGGACTAGCATTGAATAAAAAGTAATGTCTAAATCA780.4560336763330215No Hit
AGCGAGGGGGTCACACCAGGTCCCACCAGCCCCTGGACTGTGGTGTCCAGTG770.45018709073900837No Hit
AGCGAGGGGGAGTTCGGCTCAGCACCTATATCCCATCCAAGACAAAGGCACC760.4443405051449953No Hit
AGCGAGGGTATCCTACAAAGTCCAGCGTACCATAAACGCAAGCCTCAACGCA740.4326473339569691No Hit
AGCGAGGGGGAGGAAGAAGGAGAGGAATACTAAATTAAATGTCACAAGGTGC740.4326473339569691No Hit
AGCGAGGGGGTCTCCACAACTTCCCTTCATTGCGAGGTTTTATGGAAAGTGT700.4092609915809168No Hit
AGCGAGGGGACTTCTAGCATATTCTTATTTGATGCTTTCTGGAAGTACATGC700.4092609915809168No Hit
AGCGAGGGGGATCTCAGGGATTACACACCCTAAATCAGTTCAAGAGAGGCAG700.4092609915809168No Hit
AGCGAGGGGATTGTTTTCTGAAAAGTTAAAGAAGTGGGTATGCTTGTCTTTA690.4034144059869037No Hit
AGCGAGGGGAAGCTTTCTAGATGCCTAATATCCTTTGCTTAATACTTGATGG680.39756782039289057No Hit
AGCGAGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCA680.39756782039289057No Hit
AGCGAGGGGATAGTAGGTGCACATCTAGAGGGTGGGAGGCCATGAGAGAGAG670.3917212347988775No Hit
AGCGAGGGGGACGTAGTAATGTAATATTTGTAAAGCTTTGCTCTCACTGCTT670.3917212347988775No Hit
AGCGAGGGGCCCGAAGCCACTCACTCATCCTTACCTCTCTCCAGGTGAGCCT670.3917212347988775No Hit
AGCGAGGGGCTAGCGAGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCG660.38587464920486436No Hit
AGCGAGGGGACACACGTCTGAGGGACACCTTGGAGCCACGCCCTTTTTCGGC660.38587464920486436No Hit
AGCGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG650.3800280636108513No Hit
AGCGAGGGGATAGCAAGATTCTTTCCTATCCAGTTGAAATTAAAGATTTCCT640.37418147801683815No Hit
AGCGAGGGGACTCTCCCGTTGGGTCACACTAAAATTGATTTTTCCAAGCAGG630.3683348924228251No Hit
AGCGAGGGGCAATTCTGCTACCAAATATATACTCTAGTGAAAGGTTCATGCA620.36248830682881195No Hit
AGCGAGGGAACTTCCTTATTGGAGAGGACGGTCATGTCTATGAAGGCCGAGG610.35664172123479887No Hit
AGCGAGGGGAGTGAAAAAATGGGTTATAGTGAGTACTTTGAAACAACAAACT610.35664172123479887No Hit
AGCGAGGGGGATTTTGGTTCAGCCACACCCAGATCCCACCCTCTCTTTCTAG600.3507951356407858No Hit
AGCGAGGGGTGCGGGACTCTGCTGCCAGTGAGTGCTGCTGTCTCCCATGGGT600.3507951356407858No Hit
AGCGAGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTGTAAGTAGGG590.34494855004677266No Hit
AGCGAGGGGGAGAACAACCCACGTGGGCATGGCCACAGTCATGGCAAAGGCT580.3391019644527596No Hit
AGCGAGGGGCGTGCGCCTTAGTTTAGTACTCTGAATTTACTTTTAAGTCGGT580.3391019644527596No Hit
AGCGAGGGGAATATCTTTACGTGTATGTTCTGTGAGTGGCTTGGTTATTCTT570.3332553788587465No Hit
AGCGAGGGGAGCTCGACACACTAGCACCAAGAGGACACTGTCAGCCTTCCTG570.3332553788587465No Hit
AGCGAGGGGAGTCTAATTATAGCACAATGTGAGCTGTTTTTTAAAAAATCTT550.3215622076707203No Hit
AGCGAGGGGTACAAGATAAGAGCTTAATGGCTCAACTTCAAGAAACAATGCG550.3215622076707203No Hit
AGCGAGGGGATCAACATCGGCATCGGGGGCTCTGACCTGGGACCCCTCATGG550.3215622076707203No Hit
AGCGAGGGGCCCGGGCCAGCCTCCACCTCTGATGTTTGGACACGGCTGCCCG540.3157156220767072No Hit
AGCGAGGGGAAAGCCATAGATTTAAAAACAAACAAACAAACAAACAAACAAA540.3157156220767072No Hit
AGCGAGGGGACCTAAATAATTTAGTAAGAAAAATGTCCAACAGTTTTAACTA540.3157156220767072No Hit
AGCGAGGGGGATGCCGAGTGGCGCGTTTTTATGTGGACAGAATTCTACTCCA520.304022450888681No Hit
AGCGAGGGGGATCCTATGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA510.2981758652946679No Hit
AGCGAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA500.2923292797006548No Hit
AGCGAGGGGTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.2630963517305894No Hit
AGCGAGGGGTACCTATGCTTGCTAGAATCTTGGGTTTTCTGTAGCCCCACCC440.25724976613657624No Hit
AGCGAGGGGCCTTCCCCAGTCAGTCACTAATTAAGAAAATGCCCAACAGATT440.25724976613657624No Hit
AGCGAGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAAA440.25724976613657624No Hit
AGCGAGGGGGCTAGCGAGGGGAGAGTATTGCTCATCCCTCCAGAAGCAAGTC430.25140318054256316No Hit
AGCGAGGGGAGCTAATGCTCTCCAGACGTGGTTGCCAAATCACTACCTATTG410.23971000935453696No Hit
AGCGAGGGGATCTCTAAGTCCCGAGAGTTATCACTTATTTTTTTTTTAACCT410.23971000935453696No Hit
AGCGAGGGGAGAAGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGACTCT400.23386342376052385No Hit
AGCGAGGGGCCTAGAAGACAAGCCATGTGCTACAAAAGGAAAGGAATGGAGA400.23386342376052385No Hit
AGCGAGGGGATTCCCACTGTACTCGCTTCCAGCCGCCTTTACAAGTCTCTCC380.22217025257249765No Hit
AGCGAGGGGATCCAGAAGGAGTCAACCCTGCACCTGGTCCTTCGCCTGAGAG380.22217025257249765No Hit
AGCGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.21632366697848454No Hit
AGCGAGGGGACTGTGTGGCCCAGGCTGATCTCATGTCTCCTGCTCTCCTGCC360.2104770813844715No Hit
AGCGAGGGGAACCCAAGCTCCCCTCCCCCACGCCCTCGTCTTCCAACAAGCA360.2104770813844715No Hit
AGCGAGGGGATCATCTTCATCGATGAGATTGATTCTCTGTGTGGTTCCAGAA340.19878391019644528No Hit
AGCGAGGGGATAAATGAAATAAATAAATCTTCCTTTTTTAAAGTTGTTGGTT340.19878391019644528No Hit
AGCGAGGGGGTGGCTGCATGACTAAAGGCACATCCTTTTCTTAATGCAGAAT330.19293732460243218No Hit
AGCGAGGGGATTTGTGGTATGACTGTGTGTGTGTGTGTGTGTGTGTGTGTGT330.19293732460243218No Hit
AGCGAGGGGGACGGTATATTAAAGCATCTCATCTGGCCAGGTCCTGGAACCT320.18709073900841908No Hit
AGCGAGGGGAAGCCCCAGTGACTGTATTGAAGATTCTCAGGGTCACGGAGTC320.18709073900841908No Hit
AGCGAGGGGACATCAGCTTCACATCGTGGCTTTGTGTGGTTAAAAGTGCTAC320.18709073900841908No Hit
AGCGAGGGGACTTGGTACCTTGTACACTTATTACTGTCTCAGTCGTTGCTTG310.18124415341440597No Hit
AGCGAGGGGCTGAGGAAAGAGAGTCACTTGAACCCAGGAGGCAGAGGTTGCA310.18124415341440597No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.1753975678203929No Hit
AGCGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA300.1753975678203929No Hit
AGCGAGGGGGTGCAGTGCTAGCGAGGGGAAGCAGTAGTATCAATGCAAAATG290.1695509822263798No Hit
AGCGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA290.1695509822263798No Hit
AGCGAGGGGACAGAAAATGTGGTACATTTACACAACGGAGTACTACTCAGCT290.1695509822263798No Hit
AGCGAGGGGACCTGAGAACTTTTTTCGTTGCTTGGTTTTGTTTTTTTTTCTA280.16370439663236672No Hit
AGCGAGGGGAGCTCGGCACACTAGCACCAAGAGGACACTGTCAGCCTTCCTG280.16370439663236672No Hit
AGCGAGGGGATGTGGAACAATGTATTCAGTATACTTAGTGTACCTCGTTTAA280.16370439663236672No Hit
AGCGAGGGGGCTTCTAGAATAAACATACAGACTGATGGATAAACGTGTGGAT270.1578578110383536No Hit
AGCGAGGGAGCCCACACTGGTCTCCTCCGTGGCCCCATCGCAGACCAGAGCG260.1520112254443405No Hit
AGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.1520112254443405No Hit
AGCGAGGGGATGAATATAATACTCAATTTCAAGAAAAAAGTCGGGAATATGA260.1520112254443405No Hit
AGCGAGGGGGGGAATACCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG260.1520112254443405No Hit
AGCGAGGGGATGTGGGACAATGTATTCAGTATACTTAGTGTACCACGTTTAA250.1461646398503274No Hit
AGCGAGGGGGGCTTCTGCCAACCTAGAGCTGAACGGTGCACCATCTCAGTGA250.1461646398503274No Hit
AGCGAGGGGATGCCTTTGTCTCACAGGGCACTCAGTTCCTGCTCTGCACCAC250.1461646398503274No Hit
AGCGAGGGGACTTGAAAGTTCTCAGTAACTTTTTTGGCAGCTCAATAGGAAA240.1403180542563143No Hit
AGCGAGGGGGATTTACAGTCGGCAGCCCAATTCCCAACAATGGTTCAGTCGC240.1403180542563143No Hit
AGCGAGGGGGAACATATGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC240.1403180542563143No Hit
AGCGAGTCTCGCTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA230.13447146866230122Illumina Single End Adapter 2 (100% over 34bp)
AGCGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.12862488306828812No Hit
AGCGAGGGGAACAACGGGGACTAGCATTGAATAAAAAGTAATGTCTAAATCA220.12862488306828812No Hit
AGCGAGGGGGAGGAAGAAGGAGAGGAGTACTAAGTCCATTCCTTGAGCCCCC220.12862488306828812No Hit
AGCGAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG220.12862488306828812No Hit
AGCGAGGGGAGGCATTGTGTGTGTGTGTGGCATTTGTCATGATCCAGACTGC210.12277829747427502No Hit
AGCGAGGGGATCCATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCG210.12277829747427502No Hit
AGCGAGGGGGGAGGAAGACTCGTTAGGGTGAGAACTGCATTTCAAAAGGTAA190.11108512628624882No Hit
AGCGAGGGGGACTTACCCTGAAGTGGCAGCCGCTGGAGGGGGCGCCTGGTGT190.11108512628624882No Hit
AGCGAGGGGATTCTACACAAAGCAGTTGGGGGGGTATTTTTGTTTAGAAACT190.11108512628624882No Hit
AGCGAGGGAAGAAAGGACCCCCTCCTTATGGATTGCCTTGGCTTTGACCCTG190.11108512628624882No Hit
AGCGAGGGGGTTCATAGATTGGTTAACATAGTGAAAATGGCCATCTTATCAA180.10523854069223575No Hit
AGCGAGGGGGGCAGTTGCGACAGGGGCAGGGTGTCCTACCAAGGAACACTGA180.10523854069223575No Hit
AGCGAGAGGGATGTCACACGCAGATGAGATCATAGAGTATTTTTTTTTCTGC180.10523854069223575No Hit
AGCGAGGGGAAGGTAAGTCCTGACCTCAGTGGAGCCCCTCTGAGTGTGTGAA180.10523854069223575No Hit
AGCGAGGGGTGACCTATGAGTTATTCTATCCCCGGGGGTAAAAAGACTGTCT180.10523854069223575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGT700.046.0000045
TTTCTTT359.4290954E-846.00000421
TTCTTTT359.4290954E-846.00000422
GAGGGGC1450.046.0000044
GGGGACG700.046.0000047
GCCCTCG253.277101E-546.032
CTTTTGC301.7554521E-646.024
AGGGGTT301.7554521E-646.016
AGGGGTG301.7554521E-646.05
CTACAGA206.137799E-446.031
AGCCTGG301.7554521E-646.045
TGATGCT253.277101E-546.038
ATCCCCG206.137799E-446.028
CGTTTAA1000.046.046
ATCCCAT206.137799E-446.030
TGTGAGG253.277101E-546.023
AGCCCAC206.137799E-446.09
TCCTCTG206.137799E-446.035
GAGGTGC301.7554521E-646.031
AGCCTAC206.137799E-446.028
Produced by FastQC (version 0.11.5)