FastQCFastQC Report
Fri 17 Jun 2016
SRR1527190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16525
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGAACAACTGGGACTAGCATTGAATAAAAAGTAATGTCTAAATCA6253.7821482602118004No Hit
AGCGAGGGGAGCTCGTCACACTAGCACCAAGAGGACACTGTCAGCCTTCCTG5743.4735249621785176No Hit
AGCGAGGGGATGTGGAACAATGTATTCAGTATACTTAGTGTACCACGTTTAA5463.304084720121029No Hit
AGCGAGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA3462.0937972768532527No Hit
AGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG3161.9122541603630865No Hit
AGCGAGGGGAACCCACGCTCCCCTCCCCCACGCCCTCGTCTTCCAACAAGCA3001.8154311649016641No Hit
AGCGAGGGGCGTCTGATCTATGACACCAAGGGTCGCTTTGCTGTTCATCGTA2521.524962178517398No Hit
AGCGAGGGAGCCCACTCTGGTCTCCTCCGTGGCCCCATCGCAGACCAGAGCG2291.3857791225416036No Hit
AGCGAGGGGGACGGTGTATTAAAGCATCTCATCTGGCCAGGTCCTGGAACCT2291.3857791225416036No Hit
AGCGAGGGGCTGAGGCAAGAGAGTCACTTGAACCCAGGAGGCAGAGGTTGCA2021.2223903177004538No Hit
AGCGAGGGGAAGGTATGTCCTGACCTCAGTGGAGCCCCTCTGAGTGTGTGAA1630.9863842662632375No Hit
AGCGAGGGGGCTTCTTGAATAAACATACAGACTGATGGATAAACGTGTGGAT1480.8956127080181543No Hit
AGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1450.8774583963691377No Hit
AGCGAGGGGTGACCTCTGAGTTATTCTATCCCCGGGGGTAAAAAGACTGTCT1440.8714069591527988No Hit
AGCGAGGGGGGGGGATTAGCTCAGCTGGGAGAGCACCTGCTTTGCAAGCAGG1400.8472012102874432No Hit
AGCGAGGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC1320.7987897125567323No Hit
AGCGAGGGGATTTTTAGGGGTTTCTTTTGCGGGACAGAGGAACAGATCTTGA1270.7685325264750379No Hit
AGCGAGAGGCCACGGTGTATTAAAGCATCTCATCTGGCCAGGTCCTGGAACC1260.762481089258699No Hit
AGCGAGGGGGGCTTTGGACAGGGGCCAGGAAAGGGCTGAGCCGGAGGAAATG1220.7382753403933434No Hit
AGCGAGGGGGACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTC1180.7140695915279879No Hit
AGCGAGGGGGTTTTTTATTTTTTGTTTTTTTAATTGTATGTTTGTGTGTTTA1170.7080181543116489No Hit
AGCGAGGGGAGCATGGCAGGTGGAAGCTGTCCCCAGTCCCAGGCCTCAACAG1160.7019667170953102No Hit
AGCGAGGGGTATCACTGAAGGAGCTGATCTGCTGGAGGGACTTCAGGATGTG1140.6898638426626325No Hit
AGCGAGGGGCCCTTTCTGCGCAGGAAGGCTGAGGTGCTAGCACCAGCCTGGT1140.6898638426626325No Hit
AGCGAGGGGCAAAGGACTCAAGGAATTCCTAGGCTCAGGGGGCCACTGCTGT1130.6838124054462935No Hit
AGCGAGGGGATTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCA1100.6656580937972768No Hit
AGCGAGGGGGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAG1100.6656580937972768No Hit
AGCGAGGGGCGCAAGAGGAATGGGGGAAAGCAGCAATGGTGAAGCATTTCTT1030.6232980332829047No Hit
AGCGAGGGGAGTTAGAACAGCCTCTGTGGTGGGCCGGGAAGGTTTCTGAGGG1000.6051437216338881No Hit
AGCGAGGGAGGGAGGAAGGGAGGAAAGAATTGGTCAAGTCAGGTAAGAAGAA1000.6051437216338881No Hit
AGCGAGGGGGTTCATGGATTGGTTAACATAGTGAAAATGGCCATCTTATCAA1000.6051437216338881No Hit
AGCGAGGGGGTTCGCGTTTCGCCATGTCGGGGTCCCCGGTAAAGCGCCAGAG1000.6051437216338881No Hit
AGCGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA980.5930408472012103No Hit
AGCGAGGGGGAATGTTTTAATGTTGAATTTTGGACATGTTATTACTGTTCAA960.5809379727685325No Hit
AGCGAGGGGCCCGAAGCCACTCACTCATCCTTACCTCTCTCCAGGTGAGCCT950.5748865355521936No Hit
AGCGAGGGGATGCCAATTTTGTCAACAGGAAACTTCACAAAACACACCCTCC920.556732223903177No Hit
AGCGAGGGGGTCACCTCTACAATCCTGTAATGAATTGCAGCTAGCAAAGATA920.556732223903177No Hit
AGCGAGGGGGGCTGGTGGGCAGGCTAAGAGTGTGGACAGCCTGCACTCCAAG910.5506807866868382No Hit
AGCGAGGGGATGAGACTCTGTTTTGAAAAGACAAAGGGAAAAAGGAAGGTAG900.5446293494704992No Hit
AGCGAGGGGTCCTTGGCAGACATAGGTGTTTTCCCAGCTCTAGGGGTCTGAC900.5446293494704992No Hit
AGCGAGGAGGGAAGGCCGAGGCAGGCAGATCACTTGAGGTCAGAAGTTTGAG890.5385779122541604No Hit
AGCGAGGGGGTCACACCAGGTCCCACCAGCCCCTGGACTGTGGTGTCCAGTG890.5385779122541604No Hit
AGCGAGGGGGGTCGAGACTCCTCTACCCTGTGCAAAGGTGTATGAGTTTCGA870.5264750378214826No Hit
AGCGAGGGGATTGATGAACCCATACAAAGGGAAAACTCAGACGCCAGTAAGC870.5264750378214826No Hit
AGCGAGGGTATCCTACAAAGTCCAGCGTACCATAAACGCAAGCCTCAACGCA860.5204236006051437No Hit
AGCGAGGGGGCCACGGTGTATTAAAGCATCTCATCTGGCCAGGTCCTGGAAC840.5083207261724659No Hit
AGCGAGGGGGAGTTCGGCTCAGCACCTATATCCCATCCAAGACAAAGGCACC830.5022692889561271No Hit
AGCGAGGGGGACGTAGTAATGTAATATTTGTAAAGCTTTGCTCTCACTGCTT820.49621785173978816No Hit
AGCGAGGGGACTCAAAGAAAGCATGAAAGCCTACAGAGAGTCACTTTCGGAT810.4901664145234493No Hit
AGCGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG800.4841149773071104No Hit
AGCGAGGGGATAGTAGGTGCACATCTAGAGGGTGGGAGGCCATGAGAGAGAG760.4599092284417549No Hit
AGCGAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCCAAGG740.44780635400907715No Hit
AGCGAGGGGGAGGAAGAAGGAGAGGAATACTAAATTAAATGTCACAAGGTGC740.44780635400907715No Hit
AGCGAGGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTATC740.44780635400907715No Hit
AGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG730.44175491679273826No Hit
AGCGAGGGGACTTCTAGCATATTCTTATTTGATGCTTTCTGGAAGTACATGC700.4236006051437216No Hit
AGCGAGGGGAGAGTGACAGACTTGTGAGGCACCATGAGCGTTCTGGGCATTG690.4175491679273827No Hit
AGCGAGGGGGTCTCCACAACTTCCCTTCATTGCGAGGTTTTATGGAAAGTGT690.4175491679273827No Hit
AGCGAGGGGTACAAGATAAGAGCTTAATGGCTCAACTTCAAGAAACAATGCG680.41149773071104384No Hit
AGCGAGGGGACTCTCCCGTTGGGTCACACTAAAATTGATTTTTCCAAGCAGG670.405446293494705No Hit
AGCGAGGGGGAGTGCAGTGCTAGCGAGGGGTATTGTATTAAGATTTTATAGT670.405446293494705No Hit
AGCGAGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCA670.405446293494705No Hit
AGCGAGGGGCCCGGGCCAGCCTCCACCTCTGATGTTTGGACACGGCTGCCCG650.39334341906202724No Hit
AGCGAGGGGCCTCGGGAGGCTGAGGCAGGGGAATTTCTTGAACACAGGAGGC630.3812405446293495No Hit
AGCGAGGGGAGGCCCAGAGATGGCAACCACTCTCAGCCTCCAGTTCTGCTGC620.3751891074130106No Hit
AGCGAGGGGACATTCATTCTAGTTATAATAAATTTAATGTTTGTAAATGTAA620.3751891074130106No Hit
AGCGAGGGGAAGCTTTCTAGATGCCTAATATCCTTTGCTTAATACTTGATGG620.3751891074130106No Hit
AGCGAGGGGCAATTCTGCTACCAAATATATACTCTAGTGAAAGGTTCATGCA610.3691376701966717No Hit
AGCGAGGGGCGTGCGCCTTAGTTTAGTACTCTGAATTTACTTTTAAGTCGGT600.3630862329803328No Hit
AGCGAGGGGAATATCTTTACGTGTATGTTCTGTGAGTGGCTTGGTTATTCTT590.35703479576399394No Hit
AGCGAGGGGATAGCAAGATTCTTTCCTATCCAGTTGAAATTAAAGATTTCCT590.35703479576399394No Hit
AGCGAGGGGCTAGCGAGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCG590.35703479576399394No Hit
AGCGAGGGGACCTTTCTCATCTCTGTCGGTCAATGGTGGAGCCAGCTACGGG590.35703479576399394No Hit
AGCGAGGGAACTTCCTTATTGGAGAGGACGGTCATGTCTATGAAGGCCGAGG570.3449319213313162No Hit
AGCGAGGGGATTGTTTTCTGAAAAGTTAAAGAAGTGGGTATGCTTGTCTTTA570.3449319213313162No Hit
AGCGAGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAAA560.3388804841149773No Hit
AGCGAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA560.3388804841149773No Hit
AGCGAGGGGGATTTTGGTTCAGCCACACCCAGATCCCACCCTCTCTTTCTAG560.3388804841149773No Hit
AGCGAGGGGAAAGCCATAGATTTAAAAACAAACAAACAAACAAACAAACAAA540.3267776096822995No Hit
AGCGAGGGGGATCTCAGGGATTACACACCCTAAATCAGTTCAAGAGAGGCAG530.32072617246596064No Hit
AGCGAGGGGACCTAAATAATTTAGTAAGAAAAATGTCCAACAGTTTTAACTA520.3146747352496218No Hit
AGCGAGGGGAGTGAAAAAATGGGTTATAGTGAGTACTTTGAAACAACAAACT510.3086232980332829No Hit
AGCGAGGGGAGAAGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGACTCT490.29652042360060515No Hit
AGCGAGGGGACTGTGTGGCCCAGGCTGATCTCATGTCTCCTGCTCTCCTGCC490.29652042360060515No Hit
AGCGAGGGGATCAACATCGGCATCGGGGGCTCTGACCTGGGACCCCTCATGG480.29046898638426627No Hit
AGCGAGGGGACACACGTCTGAGGGACACCTTGGAGCCACGCCCTTTTTCGGC470.2844175491679274No Hit
AGCGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.2844175491679274No Hit
AGCGAGGGGATTTGTGGTATGACTGTGTGTGTGTGTGTGTGTGTGTGTGTGT460.2783661119515885No Hit
AGCGAGGGGTGCGGGACTCTGCTGCCAGTGAGTGCTGCTGTCTCCCATGGGT460.2783661119515885No Hit
AGCGAGGGGATCTCTAAGTCCCGAGAGTTATCACTTATTTTTTTTTTAACCT450.2723146747352496No Hit
AGCGAGGGGGATGCCGAGTGGCGCGTTTTTATGTGGACAGAATTCTACTCCA440.26626323751891073No Hit
AGCGAGGGGGAGAACAACCCACGTGGGCATGGCCACAGTCATGGCAAAGGCT420.25416036308623297No Hit
AGCGAGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTGTAAGTAGGG420.25416036308623297No Hit
AGCGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA410.24810892586989408No Hit
AGCGAGGGGAGTCTAATTATAGCACAATGTGAGCTGTTTTTTAAAAAATCTT390.23600605143721637No Hit
AGCGAGGGGACTTGAAAGTTCTCAGTAACTTTTTTGGCAGCTCAATAGGAAA380.22995461422087746No Hit
AGCGAGGGGATCCAGAAGGAGTCAACCCTGCACCTGGTCCTTCGCCTGAGAG370.22390317700453857No Hit
AGCGAGGGGAGCTAATGCTCTCCAGACGTGGTTGCCAAATCACTACCTATTG360.2178517397881997No Hit
AGCGAGGGGCCTTCCCCAGTCAGTCACTAATTAAGAAAATGCCCAACAGATT360.2178517397881997No Hit
AGCGAGGGGTACCTATGCTTGCTAGAATCTTGGGTTTTCTGTAGCCCCACCC350.2118003025718608No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.2118003025718608No Hit
AGCGAGGGGAACAACTGGGAGTAGCATTGAATAAAAAGTAATGTCTAAATCA340.20574886535552192No Hit
AGCGAGGGGATGAATATAATACTCAATTTCAAGAAAAAAGTCGGGAATATGA340.20574886535552192No Hit
AGCGAGGGGCCTAGAAGACAAGCCATGTGCTACAAAAGGAAAGGAATGGAGA330.19969742813918306No Hit
AGCGAGGGGGCTAGCGAGGGGAGAGTATTGCTCATCCCTCCAGAAGCAAGTC320.19364599092284418No Hit
AGCGAGGGGATCATCTTCATCGATGAGATTGATTCTCTGTGTGGTTCCAGAA320.19364599092284418No Hit
AGCGAGGGGACTTGGTACCTTGTACACTTATTACTGTCTCAGTCGTTGCTTG310.1875945537065053No Hit
AGCGAGGGGTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.1815431164901664No Hit
AGCGAGGGGAAGCCCCAGTGACTGTATTGAAGATTCTCAGGGTCACGGAGTC300.1815431164901664No Hit
AGCGAGGGGGATTTACAGTCGGCAGCCCAATTCCCAACAATGGTTCAGTCGC290.17549167927382756No Hit
AGCGAGGGGAGGCATTGTGTGTGTGTGTGGCATTTGTCATGATCCAGACTGC290.17549167927382756No Hit
AGCGAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG280.16944024205748864No Hit
AGCGAGGGGATAAATGAAATAAATAAATCTTCCTTTTTTAAAGTTGTTGGTT270.16338880484114976No Hit
AGCGAGGGGACATCAGCTTCACATCGTGGCTTTGTGTGGTTAAAAGTGCTAC270.16338880484114976No Hit
AGCGAGGGGGTGGCTGCATGACTAAAGGCACATCCTTTTCTTAATGCAGAAT260.1573373676248109No Hit
AGCGAGGGGATGTGGAACAAGGTATTCAGTATACTTAGTGTACCACGTTTAA260.1573373676248109No Hit
AGCGAGGGGGGCTTCTGCCAACCTAGAGCTGAACGGTGCACCATCTCAGTGA260.1573373676248109No Hit
AGCGAGGGGATGCCTTTGTCTCACAGGGCACTCAGTTCCTGCTCTGCACCAC260.1573373676248109No Hit
AGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.15128593040847202No Hit
AGCGAGGGGATGTGGAACAAGGTATTCAGTATACTTAGTATACCACGTTTAA250.15128593040847202No Hit
AGCGAGGGGGAGGAAGAAGGAGAGGAGTACTAAGTCCATTCCTTGAGCCCCC250.15128593040847202No Hit
AGCGAGGGGAGCTCGTCACAGTAGCACCAAGAGGACACTATCAGCCTTCCTG250.15128593040847202No Hit
AGCGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA240.14523449319213314No Hit
AGCGAGGGGGGCAGTTGCGACAGGGGCAGGGTGTCCTACCAAGGAACACTGA230.13918305597579425No Hit
AGCGAGAGGGATGTCACACGCAGATGAGATCATAGAGTATTTTTTTTTCTGC230.13918305597579425No Hit
AGCGAGGGGAACAACTGGGAGTAGCATTGAATAAAAAGTGATGTCTAAATCA230.13918305597579425No Hit
AGCGAGGGGACCTGAGAACTTTTTTCGTTGCTTGGTTTTGTTTTTTTTTCTA220.13313161875945537No Hit
AGCGAGGGGGACTTACCCTGAAGTGGCAGCCGCTGGAGGGGGCGCCTGGTGT220.13313161875945537No Hit
AGCGAGTCTCGCTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA220.13313161875945537Illumina Single End Adapter 2 (100% over 34bp)
AGCGAGGGGATCCATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCG220.13313161875945537No Hit
AGCGAGGGGATGTGGAACAATGTATTCAGTATACTTAGTGTACCTCGTTTAA220.13313161875945537No Hit
AGCGAGGGGGTGCAGTGCTAGCGAGGGGAAGCAGTAGTATCAATGCAAAATG210.12708018154311648No Hit
AGCGAGGGGGGGGATTAGCTCAGCTGGGAGAGCACCTGCTTTGCAAGCAGGG210.12708018154311648No Hit
AGCGAGGGGCGTCTGATCTAGGACACCAAGGGTCGCTTTACTGTTCATCGTA200.1210287443267776No Hit
AGCGAGGGGGGAGGAAGACTCGTTAGGGTGAGAACTGCATTTCAAAAGGTAA190.11497730711043873No Hit
AGCGAGGGGGGGAATTCCTTGGATTCTGTTTTGCTACCCGTTGTAGCGCCGG180.10892586989409984No Hit
AGCGAGGGGATTCCCACTGTACTCGCTTCCAGCCGCCTTTACAAGTCTCTCC180.10892586989409984No Hit
AGCGAGGGGGGGAATTCCTTGGATTCTGTTTTGCTACCCATTGTAGCGCCGG180.10892586989409984No Hit
AGCGAGGGGATTCTACACAAAGCAGTTGGGGGGGTATTTTTGTTTAGAAACT180.10892586989409984No Hit
AGCGAGGGGAGCTCGTCACAGTAGCACCAAGAGGACACTGTCAGCCTTCCTG170.10287443267776096No Hit
AGCGAGGGGGACGGTGTATTGAAGCATCTCATCTGGCCAAGTCCTGGAACCT170.10287443267776096No Hit
AGCGAGGGGCATGGTTGATCAGCGCCAGCAGACTGTTGATCGCCGTGGCCTG170.10287443267776096No Hit
AGCGAGGGGAACCCACGCTCGCCTCCCCCACGCCCTCGTATTCCAACAAGCA170.10287443267776096No Hit
AGCGAGGGGACAGAAAATGTGGTACATTTACACAACGGAGTACTACTCAGCT170.10287443267776096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2250.046.0000045
GCCCTCG452.728484E-1046.032
GCCCCAT206.131453E-446.032
GCCCCAG206.131453E-446.012
TTAGAAC206.131453E-446.012
AGGGGTG301.7516322E-646.05
CTCTGGT206.131453E-446.015
ATCCTGT253.2720134E-546.022
CTCTGGC253.2720134E-546.037
CAGCTAG206.131453E-446.038
TGGTCTC206.131453E-446.018
GTATGTC206.131453E-446.013
CGTTTAA800.046.046
TGTGAGT206.131453E-446.031
CTACAAT253.2720134E-546.017
TGATGAA206.131453E-446.012
AGCCTCT206.131453E-446.019
TAGAACA206.131453E-446.013
AGCCCAC206.131453E-446.09
TCCTCTG253.2720134E-546.035
Produced by FastQC (version 0.11.5)